• Asian Pacific Journal of Cancer Prevention
• /
• 제15권23호
• /
• pp.10299-10306
• /
• 2015

The esophageal squamous cell carcinoma (ESCC) is thought to develop through a multi-stage process. Epigenetic gene silencing constitutes an alternative or complementary mechanism to mutational events in tumorigenesis. Posttranscriptional regulation of human leukocyte antigen class I (HLA-I) and antigen processing machinery (APM) proteins expression may be associated with novel epigenetic modifications in cancer development. In the present study, we determined the expression levels of HLA-I antigen and APM components by immunohistochemistry. Then by a bisulfite-sequencing PCR (BSP) approach, we identified target CpG islands methylated at the gene promoter region of APM family genes in a ESCC cell line (ECa109), and further quantitative analysis of CpG site specific methylation of these genes in cases of Kazakh primary ESCCs with corresponding non-cancerous esophageal tissues using the Sequenom MassARRAY platform. Here we showed that the development of ESCCs was accompanied by partial or total loss of protein expression of HLA-B, TAP2, LMP7, tapasin and ERp57. The results demonstrated that although no statistical significance was found of global target CpG fragment methylation level sof HLA-B, TAP2, tapasin and ERp57 genes between ESCC and corresponding non-cancerous esophageal tissues, there was significant differences in the methylation level of several single sites between the two groups. Of thesse only the global methylation level of LMP7 gene target fragments was statistically higher ($0.0517{\pm}0.0357$) in Kazakh esophageal cancer than in neighboring normal tissues ($0.0380{\pm}0.0214$, p<0.05). Our results suggest that multiple CpG sites, but not methylation of every site leads to down regulation or deletion of gene expression. Only some of them result in genetic transcription, and silencing of HLA-B, ERp57, and LMP7 expression through hypermethylation of the promoters or other mechanisms may contribute to mechanisms of tumor escape from immune surveillance in Kazakh esophageal carcinogenesis.

• 한국철도학회논문집
• /
• 제10권6호
• /
• pp.742-750
• /
• 2007

본 논문은 장래 도입되는 KTX2 고속차량을 이용한 중련편성 운영계획 최적화를 위한 방법론 제시를 목적으로 한다. 중련편성 운영계획 최적화를 위한 수리적 모형을 제시하고, 제안된 수리적 모형의 적용방안을 제시한다. 특히 확률적 수요를 고려한 모형을 수립하여, 계획수립 단계에서 수요의 확률적 요소를 보다 현실적으로 반영하고, 모형내에서 좌석 서비스 수준을 직접 조정할 수 있다.

• 응용통계연구
• /
• 제7권1호
• /
• pp.83-102
• /
• 1994

컴퓨터 시뮬레이션 실험을 이용한 제반 연구의 효율성을 높이기 위한 통계적 실험 계획법으로서 최적 실험법과 라틴 하이퍼큐브 계획법에 대하여 연구하여 최적 라틴 하이퍼큐브 계획법을 제시하였다. 또한 전산 실험 자료의 분석을 위하여, 공간적 예측모형을 택하여 자료로부터의 모수추정과 이 모형에 적합한 예측방법 및 최적 실험 계획법 등이 고려되었다. 최적 라틴 하이퍼큐브 실험계획법을 구성하기 위한 2단계 (2점 교환법 및 뉴톤방법) 알고리즘과 그것에 의한 결과를 제시하였고, 나아가 축차적(최적) 라틴 하이퍼큐브 계획법의 구축을 위한 한 방법을 제시하였다. 이와같은 접근법은 주요인 그림과 축차적인 계획 및 분석을 이용하여 집적회로 계획의 최적화 문제로 응용되어 결국 품질향상에 도움이 되도록 하는 실예를 통하여 그 실제적 적용성이 예증되었다.

• 한국정보시스템학회지:정보시스템연구
• /
• 제28권4호
• /
• pp.333-358
• /
• 2019

Purpose The study aims to develop a data-based decision model for private bankers when recommending hedge funds to their customers in financial institutions. Design/methodology/approach The independent variables are set in two groups. The independent variables of the first group are aggressive investors, active investors, and risk-neutral type investors. In the second group, variables considered by private bankers include customer propensity to invest, reliability, product subscription experience, professionalism, intimacy, and product understanding. A decision-making variable for a private banker is in recommending a first-rate general private fund composed of foreign and domestic FinTech products. These contain dependent variables that include target return rate(%), fund period (months), safeguard existence, underlying asset, and hedge fund name. Findings Based on the research results, there is a 94.4% accuracy in decision-making when the independent variables (customer rating, reliability, intimacy, product subscription experience, professionalism and product understanding) are used according to the following order of relevant dependent variables: step 1 on safeguard existence, step 2 on target return rate, step 3 on fund period, and step 4 on hedge fund name. Next, a 93.7% accuracy is expected when decision-making uses the following order of dependent variables: step 1 on safeguard existence, step 2 on target return rate, step 3 on underlying asset, and step 4 on fund period. In conclusion, a private banker conducts a decision making stage when recommending hedge funds to their customers. When examining a private banker's recommendations of hedge funds to a customer, independent variables influencing dependent variables are intimacy, product comprehension, and product subscription experience according to a categorical regression model and artificial neural network analysis model.

• Journal of Advanced Marine Engineering and Technology
• /
• 제40권4호
• /
• pp.301-306
• /
• 2016

추진축계 횡진동은 축계배치계산과 함께 설계단계에서 충분한 검토가 이루어져야 한다. 축계 강제 횡진동 문제는 아직 명쾌한 해석방법이 제시되지 못하고 있으며 선급, 국제규격 등에서 대략적으로 프로펠러 날개차수 고유 진동수가 엔진정격회전수의 ${\pm}20%$이내에 들지 못하게 하고 있다. 축계배치관점에서는 축계 지지베어링 중심간 사이의 거리가 멀수록 좋으나 횡진동관점에서는 파장이 길어지고 고유진동수는 낮아져 공진이 발생할 수 있는 요인이 되어 검토시 주의가 요구되므로 계산결과를 실측으로 검증하는 것이 바람직하다. 본 연구에서는 최근 친환경 고효율 선박으로 등장한 5만 DWT급 석유화학제품운반선을 대상으로 횡진동 이론적 분석 및 스트레인 게이지 계측자료 분석을 수행하고 이를 통해 횡진동이 추진축계에 미치는 영향을 검토하였다.

• 한국자원식물학회지
• /
• 제22권3호
• /
• pp.227-235
• /
• 2009

Single seeds of common buckwheat cultivar Suwon No. 1 when subjected to SDS-PAGE revealed very high polymorphism. High variation existed for protein or protein subunits with molecular weight 54-47kDa, 45-25kDa and 16-11kDa. The electrophoregram showed variation for globulin as well as other protein fractions. About 300 proteins were separated by two-dimensional electrophoresis in common buckwheat (Fagopyrum esculentum Moench.) seed. Seed maturation is a dynamic and temporally regulated phase of seed development that determines the composition of storage proteins reserves in mature seeds. Buckwheat seeds from 5, 10, 15, 20, and 25 days after pollination and matured stage were used for the analysis. This led to the establishment of high-resolution proteome reference maps, expression profiles of 48 spots. It was identified 48 proteins from MALDI-TOF/MS analysis of wild buckwheat seed storage proteins. The 48 proteins were found identical or similar to those of proteins reported in buckwheat and other plants; it is belonging to 9 major functional categories including seed storage proteins, stress/defense response, protein synthesis, photosynthesis, allergy proteins, amino acid, enzyme, metabolism, and miscellaneous. It appears that the major allergenic storage protein separated played the important role in buckwheat breeding and biochemical characterization.

• Wind and Structures
• /
• 제17권6호
• /
• pp.647-670
• /
• 2013

The paper presents a numerical approach to study of fluid flow characteristics and to predict performance of wind turbines. The numerical model is based on Finite-volume method (FVM) discretization of unsteady Reynolds-averaged Navier-Stokes (URANS) equations. The movement of turbine blades is modeled using moving mesh technique. The turbulence is modeled using commonly used turbulence models: Renormalization Group (RNG) k-${\varepsilon}$ turbulence model and the standard k-${\varepsilon}$ and k-${\omega}$ turbulence models. The model is validated with the experimental data over a large range of tip-speed to wind ratio (TSR) and blade pitch angles. In order to demonstrate the use of numerical method as a tool for designing wind turbines, two dimensional (2-D) and three-dimensional (3-D) simulations are carried out to study the flow through a small scale Darrieus type H-rotor Vertical Axis Wind Turbine (VAWT). The flows predictions are used to determine the performance of the turbine. The turbine consists of 3-symmetrical NACA0022 blades. A number of simulations are performed for a range of approaching angles and wind speeds. This numerical study highlights the concerns with the self-starting capabilities of the present VAWT turbine. However results also indicate that self-starting capabilities of the turbine can be increased when the mounted angle of attack of the blades is increased. The 2-D simulations using the presented model can successfully be used at preliminary stage of turbine design to compare performance of the turbine for different design and operating parameters, whereas 3-D studies are preferred for the final design.

• 자원ㆍ환경경제연구
• /
• 제14권2호
• /
• pp.447-474
• /
• 2005

응답자들에 따라 지불의사액(willingness to pay : WTP) 조사에서 응답한 WTP에 대한 확신도, 즉 선호의 강도가 다를 수 있다. 본 연구는 선호강도의 정도에 대한 정보를 얻기 위해 응답자가 응답한 WTP에 대해 선호강도가 어떤지에 대한 응답을 이끌어 내었다. 선호강도를 반영한 WTP 자료의 분석을 위해 본 논문에서는 Type 3 토빗모형의 적용을 고려한다. 이 모형을 추정하기 위해서는 통상 동분산 및 이변량 정규성을 만족하는 오차항 구조를 가정한 모수적 2단계 추정법을 적용한다. 하지만 이 가정들이 만족되지 않는다면 추정치는 비일치적이게 된다. 동분산과 정규성 가설에 대해 검정한 결과 유의수준 1%에서 두 가정은 모두 기각되었다. 따라서 모수적 Type 3 토빗모형을 추정하는데 요구되는 가정은 너무 제약적이라 할 수 있다. 본 연구에서는 이 모수적 모형에 대한 대안으로 준모수적 Type 3 토빗모형을 적용한다. 분석결과 준모수적 추정은 모수적 추정보다 유의하게 우수하였으며, 더욱더 중요하게는 모수적 모형으로부터 계산된 평균 WTP 추정치는 준모수적 모형으로부터 계산된 것과 유의하게 다름을 알 수 있었다.

• 한국정밀공학회지
• /
• 제20권4호
• /
• pp.29-38
• /
• 2003

This paper deals with an automated computer-aided process planning and die design system with which designer can determine operation sequences even after only a little experience in process planning and die design of multi former-bolt products by multi-stage former working. The approach is based on knowledge-based rules, and a process knowledge base consisting of design rules is built. Knowledge fur the system is formulated from plasticity theories, empirical results and the empirical knowledge of field experts. Programs for the system have been written in AutoLISP for AutoCAD with a personal computer. An attempt is made to link programs incorporating a number of expert design rules with the process variables obtained by commercial FEM softwares, DEFORM and ANSYS, to form a useful package. The system is composed of four main modules. The process planning and die design module considers several factors, such as the complexities of preform geometry, punch and die profiles, specifications of available multi former, and the availability of standard parts. It can provide a flexible process based on either the reduction in the number of forming sequences by combining the possible two processes in sequence, or the reduction of deviation of the distribution and the level of the required forming loads by controlling the forming ratios. The system uses 2D geometry recognition and is integrated with the technology of process planning, die design, and CAE analysis. The standardization of die parts for multi former-bolt products requiring a cold forging process is described. The system developed makes it possible to design and manufacture multi former-bolt products more efficiently.

• 대한기계학회논문집
• /
• 제13권2호
• /
• pp.287-298
• /
• 1989

본 수치적 해석에서는 H$_{w}$/H$_{g}$의 비가 5.0~16.7의 범위에서 수행된 실험결과를 바탕으로 기존의 ｋ-.epsilon. 난류모델을 사용하여, 고려하고자 하는 변수, 즉 유입유속, 입구 게이트의 높이, 수위, 배플의 유.무등에 따라서 액체의 유동형태가 어떻게 변하는가를 살펴 보고자 한다.다.