• Title/Summary/Keyword: Two layer

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Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial

  • Jeong, Cheol;Chung, Ho Yun;Lim, Hyun Ju;Lee, Jeong Woo;Choi, Kang Young;Yang, Jung Dug;Cho, Byung Chae;Lim, Jeong Ok;Yoo, James J.;Lee, Sang Jin;Atala, Anthony J.
    • Archives of Plastic Surgery
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    • v.41 no.6
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    • pp.661-667
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    • 2014
  • Background Tissue expansion is an effective and valuable technique for the reconstruction of large skin lesions and scars. This study aimed to evaluate the applicability and safety of a newly designed skin expanding bioreactor system for maximizing the graft area and minimizing the donor site area. Methods A computer-controlled biaxial skin bioreactor system was used to expand skin in two directions while the culture media was changed daily. The aim was to achieve an expansion speed that enabled the skin to reach twice its original area in two weeks or less. Skin expansion and subsequent grafting were performed for 10 patients, and each patient was followed for 6 months postoperatively for clinical evaluation. Scar evaluation was performed through visual assessment and by using photos. Results The average skin expansion rate was $10.54%{\pm}6.25%$; take rate, $88.89%{\pm}11.39%$; and contraction rate, $4.2%{\pm}2.28%$ after 6 months. Evaluation of the donor and recipient sites by medical specialists resulted in an average score of 3.5 (out of a potential maximum of 5) at 3 months, and 3.9 at 6 months. The average score for patient satisfaction of the donor site was 6.2 (out of a potential maximum of 10), and an average score of 5.2 was noted for the recipient site. Histological examination performed before and after the skin expansion revealed an increase in porosity of the dermal layer. Conclusions This study confirmed the safety and applicability of the in vitro skin bioreactor, and further studies are needed to develop methods for increasing the skin expansion rate.

Study on the Increase of Egg Weight and Early Selection based on Part Record in Layer (산란종계의 란중 증대성과 조기 선발에 관한 연구)

  • Park, Y.H.;Ohh, B. K.
    • Korean Journal of Poultry Science
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    • v.10 no.2
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    • pp.75-81
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    • 1983
  • The records on 787 white Leghorn hens from two different strains, A and K were wed in this study to find out the pattern of increase in egg weight with age and the optimum age of early selection for maximizing the genetic gain. The pattern of increase in egg weight with age in two lines which was expressed on graph showed that the amount of increase was larger in the early part of the laying year and it decreased gradually. Regression equations suggested that egg weight increased linearly with age at the rate of 0.46 gram per week for line A and 0.83 gram for line K. And in the regression analyses of each period mean egg weight on annual mean egg weight, the coefficients of EW 30 that is mean egg weight from 29 weeks of age to 32 weeks of age were 0.86 for line A, 0.88 for line K, and those were the highest figures among the all regression coefficients of each mean egg weight on annual mean egg weight. In estimating the relative selection efficiency of each period mean egg weight on annual mean egg weight in EW 46, selection on EW 34 for line A and on EW 30 for line K was the most efficient for improving egg weight. These results suggested that one periods from 29 weeks of age to 36 weeks of age could be used for the best predictable time for improving annual mean egg weight.

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Effect of Selective Withdrawal on the Control of Turbidity Flow and Its Water Quality Impact in Deacheong Reservoir (선택취수에 따른 대청호 탁수 조절효과 및 수질영향 분석)

  • Jung, Yong-Rak;Liu, Huan;Kim, Yu-Kyung;Ye, Lyeong;Chung, Se-Woong
    • Journal of Korea Water Resources Association
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    • v.40 no.8
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    • pp.601-615
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    • 2007
  • A selective withdrawal method has been widely used to control the quality of water released from a stratified reservoir and to improve downstream ecosystem habitats. Recently, several existing reservoir withdrawal facilities have been modified to accommodate multi-level water intake capabilities in order to adapt the impact of long-term discharge of high turbidity flow. The purpose of this study was to assess the effect of selective withdrawal method on the control of downstream turbidity and its impact on water quality in Daecheong Reservoir. A laterally integrated two-dimensional hydrodynamic and eutrophication model, which was calibrated and validated in the previous studies, was applied to simulate the temporal variations of outflow turbidity with various hypothetical selective withdrawal scenarios. In addition, their impacts on the algal growth as well as water quality constituents were analyzed in three different spatial domains of the reservoir The results showed that the costly selective withdrawal method would provide very limited benefits for downstream turbidity control during two years of consecutive simulations for 2004-2005. In particular, an excessive withdrawal from the epilimnion zone for supplying upper layer clean water resulted in movement of turbidity plume that contained high phosphorus concentrations upward photic zone, and in turn increased algal growth in the lacustrine zone.

Characteristics of Core Sedimentary Facies at the Ulleung Basin in the East Sea of Korea (한국 동해 울릉분지 코어 퇴적상 특성)

  • Lee, Byoung-Kwan;Lee, Su-Woong;Kim, Hong-Tae;Kim, Seok-Yun
    • Journal of Environmental Science International
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    • v.20 no.7
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    • pp.829-837
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    • 2011
  • A study on the grain size change, sedimentary facies and age indicator of volcanic tephra was analysis through four cores (P1 ~ P4) at the Ulleung Basin in the East Sea of Korea. The two cores (P1 and P2) were collected in the northeastern side of the Ulleung Basin (about 2,000 m in water depth), while the other two cores (P3 and P4) with the water depth of about 1,500 m and 1,700 m, respectively, were collected from the continental slope of the southwestern and western side of the Ulleung Basin. Four sedimentary facies and eight sedimentary subfacies were identified. The four facies were massive sand, bioturbated mud, homogeneous mud, and laminated mud. The eight subfacies were further divided as pumiceous ash massive sand, scorieaous massive sand, plain bioturbated mud, pyrite filamented bioturbated mud, distinctly laminated mud, indistinctly laminated mud, thinly laminated mud and homogeneous mud. The homogeneous mud was not found in the core of P3 which is located in the western side of Ulleung Basin (close to the Korean coast). In the case of laminated mud facies, the thinly laminated mud facies was dominated in the lower part of core sequences of the Ulleung Basin (P1 and P2), while the indistinctly laminated mud were overally distributed in the core sequences from the continental slope of Ulleung Basin. The Tephra layers from the core sequences of central Ulleung Basin were more dominated and distinctive than those from the core sequences of continental slope. This is related to the distance from the volcanic source and the amount of sediment supply. The core locations of Ulleung-Oki Tephra layers in the central Ulleung Basin were in the upper part of core sequences, while those in the continental slope were in the lower part of core sequences. This is indicated that the amounts of sediment supply in the continental slope after the Ulleung-Oki eruption were very high and different sedimentary environment between upper and lower of Tephra layer.

Surface Treatments of Bronze Mirrors Excavated from Korean Peninsula (한반도 출토 청동거울의 표면처리 기법에 관한 연구)

  • Jeon, Ik-Hwan;Lee, Jae-Sung;Baek, Ji-Hye;Park, Jang-Sik
    • Journal of Conservation Science
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    • v.22
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    • pp.87-98
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    • 2008
  • Microstructures and chemical compositions of 24 bronze mirrors recovered from the Korean peninsula were examined using the scanning electron microscope equipped with the energy dispersive spectrometer in an effort to characterize the treatments applied on their surface. Their provenance and chronology are mostly unspecified except for two objects from a Koryo burial site. In antiquity the surface of bronze mirrors was frequently finished by mere polishing when their tin content was high enough to guarantee the required reflectivity. In many cases, however, their surface was given a special treatment. The most typical treatment was to coat the surface with tin in two different processes referred to as wipe-tinning and amalgam-tinning. In wipe-tinning only tin was used, but in amalgam-tinning tin and mercury were used together. The surface was often coated with mercury in a process known as mercury-polishing. The present mirrors showed that all these techniques were in fact practiced, not only on the reflective surface but, in some cases, on the decorative surface. The detection of mercury played a crucial role in the assessment of a specific technique applied in each mirror. Mercury often remained in the substrate in the form of sulfide and thereby allowed the method of surface treatment to be estimated even when the coated layer was completely lost. The future study is expected to uncover the regional and temporal variation of the surface treatments to the better understanding of bronze mirrors with respect to provenance and chronology.

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Isolation of Glucoamylase Producing Yeasts and its Enzymatic Characteristics (Glucoamylase 생성효모의 분리와 효소학적 특성)

  • Park, So-Young;Choi, Soon-Young;Min, Kyung-Hee
    • The Korean Journal of Mycology
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    • v.27 no.6 s.93
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    • pp.386-393
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    • 1999
  • Ethanol is considered as one of the most suitable substitutes for the petroleum, since it offers attractive functional features at an economical cost. Glucoamylase producing yeasts were isolated and characterized. Based on the morphological character, carbon fermentations, assimilation of carbon and nitrate, growth on vitamine-free medicine, and urease activity, five isolates of Saccharomyces diastaticus, two isolates of Saccharomycopsis fibuligera, and two of Schwanniomyces occidentalis, and each isolate of Ambrosiozyma monospora and Lipomyces kononenkoae were identified. Among 12 isolates, one of the S. diastaticus, E3 showed the highest activity of glucoamylase and identified as Saccharomyces diastaticus. The hydrolysis of starch by the E3 strain showed the release of considerable amount of reducing sugar, along with the reduction in iodine staining capacity. The product of action of glucoamylase, glucose was determined by thin-layer chromatography. The enzyme activity was found to be stable in broad pH range of $5.0{\sim}7.0$ with optimal activity at pH $5.0{\sim}6.0$. The enzyme showed optimal antivity at $50^{\circ}C{\sim}60^{\circ}C$. Soluble starch and glucose were better carbon sources for the enzyme production than xylose and glycerol. $Na^+\;and\;Mg^{2+}$ increased the glucoamylase activity, however $Hg^{2+}\;and\;Ag^{2+}$ inhibited the activity. Soluble starch was the best substrate for the enzyme activity.

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Flank Reconstruction of Large Soft Tissue Defect with Reverse Pedicled Latissimus Dorsi Myocutaneous Flap: A Case Report (옆구리 부위의 거대 연부조직 결손에 대한 역넓은등근 근육피부피판을 이용한 치험례)

  • Song, Seung-Yong;Kim, Da-Han;Kim, Chung-Hun
    • Archives of Plastic Surgery
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    • v.38 no.6
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    • pp.894-898
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    • 2011
  • Purpose: Coverage of full-thickness large flank defect is a challenging procedure for plastic surgeons. Some authors have reported external oblique turnover muscle flap with skin grafting, inferiorly based rectus abdominis musculocutaneous flap, and two independent pedicled perforator flaps for flank reconstruction. But these flaps can cover only certain portions of the flank and may not be helpful for larger or more lateral defects. We report a case of large flank defect after resection of extraskeletal Ewing's sarcoma which is successfully reconstructed with reverse latissimus dorsi myocutaneous flap. Methods: A 24-year-old male patient had $13.0{\times}7.0{\times}14.0$ cm sized Ewing's sarcoma on his right flank area. Department of chest surgery and general surgery operation team resected the mass with 5.0 cm safety margin. Tenth, eleventh and twelfth ribs, latissimus dorsi muscle, internal and external oblique muscles and peritoneum were partially resected. The peritoneal defect was repaired with double layer of Prolene mesh by general surgeons. $24{\times}25$ cm sized soft tissue defect was noted and the authors designed reverse latissimus dorsi myocutaneous flap with $21{\times}10$ cm sized skin island on right back area. To achieve sufficient arc of rotation, the cephalic border of the origin of latissimus dorsi muscle was divided, and during this procedure, ninth intercostal vessels were also divided. The thoracodorsal vessels were ligated for 15 minutes before divided to validate sufficient vascular supply of the flap by intercostal arteries. Results: Mild congestion was found on distal portion of the skin island on the next day of operation but improved in two days with conservative management. Stitches were removed in postoperative 3 weeks. The flap was totally viable. Conclusion: The authors reconstructed large soft tissue defect on right flank area successfully with reverse latissimus dorsi myocutaneous flap even though ninth intercostal vessel that partially nourishes the flap was divided. The reverse latissimus dorsi myocutaneous flap can be used for coverage of large soft tissue defects on flank area as well as lower back area.

Comparison of Observation Performance of Urban Displacement Using ALOS-1 L-band PALSAR and COSMO-SkyMed X-band SAR Time Series Images (ALOS-1 L-band PALSAR와 COSMO-SkyMed X-band SAR 시계열 영상을 이용한 도심지 변위관측 성능 비교 분석)

  • Choi, Jung-Hyun;Kim, Sang-Wan
    • Korean Journal of Remote Sensing
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    • v.34 no.2_1
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    • pp.283-293
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    • 2018
  • We applied PSInSAR to two SAR satellite (ALOS-1 and COSMO-SkyMed) images and analyzed the difference in displacement observation performance according to sensor characteristics. The building layer was extracted from the digital topographic map, and the PS extracted from the SAR image was classified into two groups(building structure and ground surface) for density analysis. The density of PS extracted from the research area was $0.023point/m^2$ for ALOS-1 PALSAR and $0.1point/m^2$ for COSMO-SkyMed, more than 4 times PS was extracted compared to ALOS-1. In addition, not only the PS density in the building, but also the density in the ground were greatly increased. The average displacement velocity of ALOS-1 PALSAR is within ${\pm}1cm/yr$, while for COSMO-SkyMed it is within ${\pm}0.3cm/yr$. Although it is difficult to make quantitative comparisons because it does not use the data for the same period, it can be said that the accuracy of X-band SAR system is very high compared to the L-band. In consideration of PS observation density and observation accuracy of displacement, X-band SAR data is very effective in research where it is important to acquire useful signals from the ground surface, such as ground subsidence and sinkhole.

Nonlinear Analysis of Nuclear Reinforced Concrete Containment Structures under Accidental Thermal Load and Pressure (온도 및 내압을 받는 원자로 철근콘크리트 격납구조물의 비선형해석)

  • Oh, Byung Hwan;Lee, Myung Gue
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.14 no.3
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    • pp.403-414
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    • 1994
  • Nonlinear analysis of RC containment structure under thermal load and pressure is presented to trace the behaviour after an assumed LOCA. The temperature distribution varying with time through the wall thickness is determined by transient finite element analysis with the two time level scheme in time domain. The layered shell finite elements are used to represent the containment structures in nuclear power plants. Both geometric and material nonlinearities are taken into account in the finite element formulation. The constitutive relation of concrete is modeled according to Drucker-Prager yield criteria in compression. Tension stiffening model is used to represent the tensile behaviour of concrete including bond effect. The reinforcing bars are modeled by smeared layer at the location of reinforcements accounting elasto-plastic axial behaviors. The steel liner model under Von Mises yield criteria is adopted to represent elastic-perfect plastic behaviour. Geometric nonlinearity is formulated to consider the large displacement effect. Thermal stress components are determined by the initial strain concept during each time step. The temperature differential between any two consecutive time steps is considered as a load incremental. The numerical results from this study reveal that nonlinear temperature gradient based on transient thermal analysis will produces excessive large displacement. Nonlinear behavior of containment structures up to ultimate stage can be traced reallistically. The present study allows more realistic analysis of concrete containment structures in nuclear power plants.

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Contents of Monosaccharides in the Hydrolysates of Some Forest Soil Horizons (삼림토양(森林土壤)의 층위별(層位別) 가수분해물중(加水分解物中) 단당류(單糖類)의 함량(含量))

  • Kim, Jeong-Je;Jang, Yong-Seon;Shin, Young-Oh
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.3
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    • pp.191-196
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    • 1989
  • Monosaccharide content of four forest soils were analyzed. Two soils under coniferous forest trees and another two under duciduous forest trees of Mts. Zeombong and Odae in Kangweon-Do were sampled from the surface horizon down into the subhorizons. 1. The largest amount of monosaccharide is found in the surface organic horizon of each soil and with increasing depth the amount decreases as might be expected considering total organic matter content. 2. Hexoses (galactose, glucose, mannose) predominate over pentoses (arabinose, ribose, xylose) and deoxyhexoses (fucose, rhamnose), the latter being in the smallest amount. Glucose is the most abundant monosaccharide in all samples regardless of vegetation of soil or depth. In general the content of each monosaccharide follows the order of glucose > manrtose > galactose > arabinose > xylose > rhamnose > fucose > ribose. 3. Very little amount of ribose is present even in organic horizons of coniferous forest soils. In samples taken from deciduous forest soils ribose is virtually absent. 4. The relative proportion of monosacchaiide to the total soil organic matter decreases with increasing depth, which may be resulted from the effect of prolonged humification. The total monosaccharide in the organic surface layer amounts to 27-50% of the total organic carbon or 15.7-29% of the total organic matter. Hexoses alone take the largest share of 20-38% of the carbon, or 12-22% of the organic matter.

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