• The Bulletin of The Korean Astronomical Society
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• v.46 no.1
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• pp.47.4-48
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• 2021

Gravitationally lensed Type Ia supernovae may be the next frontier in cosmic probes, able to deliver independent constraints on dark energy, spatial curvature, and the Hubble constant. Measurements of time delays between the multiple images become more incisive due to the standardized candle nature of the source, monitoring for months rather than years, and partial immunity to microlensing. While currently extremely rare, hundreds of such systems should be detected by upcoming time-domain surveys. Others will have the images spatially unresolved, with the observed lightcurve a superposition of time delayed image fluxes. We investigate whether unresolved images can be recognized as lensed sources given only lightcurve information and whether time delays can be extracted robustly. We develop a method that we show can identify these systems for the case of lensed Type Ia supernovae with two images and time delays exceeding ten days. When tested on such an ensemble the method achieves a false positive rate of ≲5%, and measures the time delays with the completeness of ≳93% and with a bias of ≲0.5% for time delay ≳10 days. Since the method does not assume a template of any particular type of SN, the method has the potential to work on other types of lensed SNe systems and possibly on other transients.

• Journal of the Korea institute for structural maintenance and inspection
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• v.6 no.3
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• pp.97-102
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• 2002

Two dimensional Analysis has been applied to most of tunnel lining design in these days. Two dimensional analysis uses beam or curved beam element for finite element method. But because the behaviors of tunnel concrete lining structure is near to shell, it is required to model the tunnel lining as shell structure for safety design of tunnel lining structure. In this paper, two dimensional analysis by beam element and the three dimensional analysis by shell element of tunnel concrete lining are studied, in which 3 type of tunnel lining and lateral pressure factors are considered. As results of the study, three dimensional analyses of the behavior of tunnel concrete lining structure considering lateral pressure factor shows that the moment of three dimensional analysis is greater than those of two dimensional analysis. The results shows that three dimensional analysis is necessary for safety design of tunnel lining.

• Proceedings of the Korean Environmental Health Society Conference
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• 2002.04a
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• pp.49-51
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• 2002

To identify and evaluate the dichlorobenzidine(DCB)-DNA adducts in liver cell and bladder epithelial cells by $^{32}$ P-postlabeling and GC/MS-SIM, we orally exposed the dichlorobenzidine (20mg/kh body wt.,/day)to male sprague-dawley rats for 14 days. Two kinds of DCB-DNA adduct were found at the same site of thin layer chromatogram of $^{32}$ P-postlabeling method in liver cells and bladder epithelial cells. In liver cells, relative adduct labeling(RAL) $\times$ 10$^{12}$ of DCB-DNA adduct A1 were 34.1$\pm$3.71 and 69.9$\pm$5.02, that of adduct A2 were 74.1$\pm$10.1 and 105.1$\pm$10.1 on 10 and 14 days after treatment, respectively. And in bladder epithelia cells, RAL $\times$ 10$^{12}$ of DCB-DNA adduct A1 were 5.92$\pm$1.60 and 15.9$\pm$1.31, that of adduct A2 were 9,81$\pm$2.81 and 22.8$\pm$1.79 on 10 and 14 days after treatment, respectively. DCB metabolites formed DNA adducts were monoacetyl-dichlorobenzidine(acDCB) and diacety1-dichlorobenzidine(di-acDCB), which was identify by gas chromatography/mass spectrometry-scan ionization mode(GC/MS-SIM), along with hydrolysis, extraction and TFA(trifluoroacetyl anhyride) derivatization with DCB-DNA adducts isolated from live cells and bladder epithelial cells. The base peak of acDCB were 252 and 294 m/z, and that of di-acDCB were 252, 294 and 336 m/z. In conclusion, the exposed DCB formed two kinds of DCB-DNA adduct, the proximate materials of that were acDCB and di-acDCB in liver and bladder epithlial cells. And the above GC/MS-SIM method was found the DCB-DNA adducts could be monitoring by gas chromatography.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.272-278
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• 2011

Bovine whey protein expression patterns of colostrum are much different from that of milk. Moreover, bovine colostrum is an important source of protective, nutritional and developmental factors for the newborn. However, to our knowledge, no research has been performed to date using a comparative proteomic method on the changes in the bovine whey proteome during the transition from colostrum to milk. This study therefore separated whey protein of days 1, 3, 7 and 21 after calving using two dimension electrophoresis. Differentially expressed proteins at different collection times were identified using high-performance liquid chromatography in tandem with mass spectrometry (LC/MS) and validated by enzyme-linked immunosorbent assay (ELISA) in order to understand the developmental changes in the bovine whey proteome during the transition from colostrum to milk. The expression patterns of whey protein of days 1 and 3 post-partum were similar except that immunoglobulin G was down-regulated on day 3, and four proteins were found to be down-regulated on days 7 and 21 compared with day 1 after delivering, including immunoglobulin G, immunoglobulin M, albumin, and lactotransferrin, which are involved in immunity and molecule transport. The results of this study confirm the comparative proteomic method has the advantage over other methods such as ELISA and immunoassays in that it can simultaneously detect more differentially expressed proteins. In addition, the difference in composition of milk indicates a need for adjustment of the colostrum feeding regimen to ensure a protective immunological status for newborn calves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.1
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• pp.13-19
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• 1996

This study was conducted to establish the rapid determination method for major components of maturing covered barley grains, and to improve the efficiency of selection in barley breeding. Near Infrared Reflectance Spectroscopy (NIRS) is an established, economical and nondestructive technique applied widely to the food and feed industry. 34 barley lines were sampled at 5 day-interval from 25 to 35 days after heading. A standard regression analysis for the data obtained by analytical laboratory methods and NIRS method was carried out to get a useful calibration equation. The simple significant correlation between these two methods at 25 days after heading was recognized in starch and $\beta$-glucan contents. At 30 days after heading the data obtained by two methods showed significant correlation in starch, $\beta$-glucan and protein contents. Analyzed data and that from NIRS method at 35 days after heading was significantly correlated in starch and protein contents. It was concluded that the applicability of NIRS method for the components analysis in maturing barley grains was different depending on maturing stages and components.

• Journal of Korean Academy of Nursing Administration
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• v.14 no.3
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• pp.249-259
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• 2008

Purpose: This study was performed to identify the patient characteristics significantly affecting nursing outcomes and their predictability in gastrointestinal surgery patients. Method: The subjects were 149 abdominal surgery patients from 3 general surgical nursing units of 3 general hospitals. Two instruments were used to measure nursing outcomes and acuity of the subjects. The nursing outcomes were measured at post-operation 4 and 7days using review of patients' records, observation of patients, and interviews with patients by a trained nurse. For data analysis, T-test or ANOVA, Pearson Correlation and Stepwise Multiple Regression were done. Result: Age, severity score, diagnosis, cancer or not, operation site were the subjects' characteristics that were significantly related to the nursing outcomes in both post-operation 4 and 7days. Cancer or not, age, diagnosis and severity score were the significant predictors for the scores of nursing outcome in post-operation 4days and the predictability was 34.9%. The predictability of cancer or not was highest, 22.6%. Age, diagnosis and cancer or not were the significant predictors for the scores of nursing outcome in post-operation 7days and the predictability was 27.8%. The predictability of age was highest, 17.3%. Conclusions: The patient characteristics affecting nursing outcomes should be considered when nursing care is planned and provided. Especially, careful attention should be given to the patients with cancer and older age. And, these patient characteristics should be adjusted for correct estimation of the effectiveness of nursing interventions on nursing outcomes.

• Journal of Astronomy and Space Sciences
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• v.19 no.3
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• pp.197-206
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• 2002

NORAD Two Line Elements (TLE) are widely used for the increasing number of small satellite mission operations and analysis. However, due to the irregular periodicity of generation of the NORAD TLE, a new TLE that is independent of NORAD is required. A TLE type Orbit Determination (TLEOD) has been developed for the generation of a new TLE. Thus, the TLEOD system can provide an Antenna Control Unit (ACU) with the orbit determination result in the type of a TLE, which provides a simple interface for the commercialized ACU system. For the TLEOD system, NORAD SGP4 was used to make a new orbit determination system. In addition, a least squares method was implemented for the TLEOD system with the GPS navigation solutions of the KOMPSAT-1. Considering both the Orbit Propagation (OP) difference and the tendency of $B^{*}$ value, the preferable span of the day in the observation data was selected to be 3 days. Through the OD with 3 days observation data, the OP difference was derived and compared with that of Mission Analysis and Planning (MAPS) for the KOMPSAT-1. It has the extent from 2 km after sit days to 4 km after seven days. This is qualified enough for the efficiency of an ACU in image reception and processing center of the KOMPSAT-2.

• Korean Journal Plant Pathology
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• v.13 no.2
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• pp.105-112
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• 1997

Didymella bryoniae, gummy stem blight fungus of cucurbits, has been known not to produce its pycnidium in vitro without irradiation. Various methods for producing pycnidiospores of the fungus as an inoculum have been used. However, those methods have not been verified in terms of efficiency of the productivity, activity and synchronous maturation of the inoculum. Therefore, a pycnidiospore production method in vitro that is highly reliable and reproducible has to be developed to obtain a large amount of inoculum for screening disease resistant varieties or effective fungicides. Here we standardized a mass-production technique for pycnidiospores of D. bryoniae in vitro by comprehensively finding the optimal conditions such as kinds and thickness of cultural medium, growing temperature, and quality and duration of irradiation as well as examining the activity and pathogenicity of the pycnidiospores reproduced. In brief, mycelial colony on the PDA plate was cultured at 26$^{\circ}C$ for 2 days under the darkness, and then the plate was irradiated under the UV light (12 hr/a day) for 2~3 days at the same temperature(26$^{\circ}C$). Two days after UV irradiation, a great number of pycnidia was simultaneously formed. This plate was subjected to darkness again for 4~5 days to mature pycnidiospores. We could obtain a large amount of inoculum that is synchronously matured in a short period of time through the above procedures.

• Journal of Korean Institute of Industrial Engineers
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• v.26 no.1
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• pp.9-16
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• 2000

In these days, the interior interface design for vehicle drivers was recognized as important affairs. Thereby, many studies are being performed for this. These studies emphasize the physical factors and usability of human, but those for the cognitive factors are not enough. Cognitive factors are very important elements to determine the drivers' performance. In this study, it was studied about the method to extract a driver's cognitive map on IP(Instrument Panel) in dynamic situation, and BPM(Blind-Pointing Method) was proposed for this. The BPM is the method to extract a cognitive map by subject's pointing action under the blinded condition. The experiment was conducted to validate compatibility of BPM as the method to extract a cognitive map. In the experiment, subjects were divided in two groups, the first group of subjects has their own vehicle and driver license, and the second group of subjects doesn't have own vehicle but has driver license. The result shows that the IP form of cognitive map is not different between two groups, and BPM is the compatible method to extract a cognitive map.

• Journal of Microbiology and Biotechnology
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• v.33 no.3
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• pp.348-355
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• 2023

Epifluorescence microscopy with image analysis was evaluated as a biofilm quantification method (i.e., quantification of surface area colonized by biofilms), in comparison with crystal violet (CV) staining. We performed different experiments to generate multispecies biofilms with natural and artificial bacterial assemblages. First, four species were inoculated daily in 16 different sequences to form biofilms (surface colonization, 0.1%-56.6%). Second, a 9-species assemblage was allowed to form biofilms under 10 acylase treatment episodes (33.8%-55.6%). The two methods comparably measured the quantitative variation in biofilms, exhibiting a strong positive relationship (R² ≥ 0.7). Moreover, the two methods exhibited similar levels of variation coefficients. Finally, six synthetic and two natural consortia were allowed to form biofilms for 14 days, and their temporal dynamics were monitored. The two methods were comparable in quantifying four biofilms colonizing ≥18.7% (R² ≥ 0.64), but not for the other biofilms colonizing ≤ 3.7% (R² ≤ 0.25). In addition, the two methods exhibited comparable coefficients of variation in the four biofilms. Microscopy and CV staining comparably measured the quantitative variation of biofilms, exhibiting a strongly positive relationship, although microscopy cannot appropriately quantify the biofilms below the threshold colonization. Microscopy with image analysis is a promising approach for easily and rapidly estimating absolute quantity of multispecies biofilms.