• BMB Reports
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• 제32권5호
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• pp.492-496
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• 1999

We have investigated the biochemical properties of the herpes simplex virus type 1 (HSV-1) DNA polymerase without the UL42 protein (Pol), purified from insect cells infected with a recombinant baculovirus containing the UL30 gene. BSA and DTT have inhibitory effects on dAMP incorporation. Pol showed a greater turnover rate of steady-state single nucleotide incorporation at 12 mM $MgCl_2$ than at 2 mM $MgCl_2$. However, it showed a greater processivity of DNA synthesis at lower $MgCl_2$ concentration (1 mM, 2 mM) than at a higher $MgCl_2$ concentration (12.5 mM). These results are consistent with a slow DNA dissociation at lower $MgCl_2$ concentrations. Pol does not incorporate a correct nucleotide into the primer with an incorrect nucleotide at the end; instead, it preferentially excises the incorrect nucleotide at the 3' end of the primer. Pol has DNA polymerase activity at pHs 6.5 and 7.5 but little at pHs 5.5, 8.5, and 9.5. It has exonuclease activity at pHs 6.5, 7.5, and 8.5 but little at pHs 4.5, 5.5, and 9.5. The finding that Pol has exonuclease activity but not DNA polymerase at pH 8.5 suggests that DNA binds to Pol, but deoxynucleotide binding or incorporation does not occur at pH 8.5.

• 한국안전학회지
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• 제26권2호
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• pp.77-82
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• 2011

Recently, personal characteristics of organizational members arc regarded as an important factor that affects performance of organization. In addition, job stress is attributed to one of main factors that result in poor work ability and high turnover rate. In this study, job stress, work ability and DISC personality types were surveyed targeting 292 of foreman in shipbuilding company. The relationships between job stress and personal characteristics including personality types and correlation between job stress and work ability were analyzed based on the result of survey. As a result of statistical analysis, there were six statistically significant relationships between job stress and age, job tenure, work type, hobbies, exercise, personality types. On the other hand, there was a statistically significant relationship between work ability and exercise. Furthermore, negative correlation between job stress and work ability were found. In accordance with these analysis, this study presented how to improve work ability by managing job stress items and personal characteristics that affect the stress and the stress level.

• Reproductive and Developmental Biology
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• 제29권4호
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• pp.259-267
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• 2005

The present study was conducted to investigate gelatinolytic activities in HAM and to determine whether there are any changes in gelatinolytic activity profiles when the cells are cultured in hepatogenic medium. Placenta was obtained during caesarean section of the volunteers, with informed consent. HAM were isolated from amniotic membrane using collagenase type A HAM were cultured in hepatogenic medium for 3 weeks and the conditioned media were obtained at day 7, 14 and 21. The zymographic pattern of gelatinolytic activity of the HAM did not undergo a change during passages. When the HAM were cultured in a fibronectin-coated dishes in a hepatogenic medium, there was no significant difference of the gelatinase pattern between before and after culture. However, when bFGF was added to the culture, a dramatic increase of 62kDa and 59kDa gelatinases was observed. Interestingly, when ITS instead of FN was present, HAM-conditioned medium also showed a similar increase of both gelatinases. Immunoblotting analysis demonstrated that both 62kDa and 59kDa gelatinases were the active form of MMP-2 resulting from the turnover of MMP-2 proform. Futher study will be necessary to determine the relationship between bFGF and active MMP-2 during hepatogenesis of HAM.

• 대한토목학회논문집
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• 제34권6호
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• pp.1893-1899
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• 2014

본 연구는 노무중심의 전문건설업체의 경영성과지표와 건설경기지표와의 상관성 분석을 통해 노무중심의 전문건설업체의 대응전략을 제시하는 것을 목적으로 수행되었다. 노무중심의 전문건설업체는 건설수주액이 유동자산회전율에 장기적으로 영향을 미치고 있다. 이는 인력확보를 위해 유동자산을 운용하고 있기 때문인 것으로 판단된다. 즉, 노무중심의 전문건설업체는 특정 자재 혹은 장비에 대한 확보가 아닌 근로자의 수급에 초점을 맞추고 있기 때문에, 원활한 인력수급을 위해 유동부채 해결을 위한 유동자산을 운영하여 매출액 증가를 도모하기 때문인 것으로 판단된다.

• 대한간호학회지
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• 제47권5호
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• pp.676-688
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• 2017

Purpose: The purpose of this study was to analyze the migration patterns of new nurses and experienced nurses and to identify the factors influencing inter-regional migration for solving regional imbalances of clinical nurses in South Korea. Methods: This study involved a secondary analysis of data from the Health Insurance Review and Assessment Service (HIRA). Data were analyzed using descriptive statistics and multiple logistic regression analysis. Results: New nurses tended to migrate from Kyunggi to Seoul. However, experienced nurses tended to migrate from Seoul and Chungchung to Kyunggi. Significant predictors of inter-regional migration among new nurses were location and nurse staffing grade of hospitals. Significant predictors of inter-regional migration among experienced nurses were location, hospital type, nurse staffing grade, ownership of hospitals and age of nurses. Conclusion: Inter-regional migration occupied a small portion of total hospital movement among clinical nurses. The regional imbalances of nurses were not caused by the migration from non-metropolitan areas to Seoul. Nurse shortage problems in the small and medium hospitals of the non-metropolitan area can be solved only through improvement of work environment.

• 보건의료산업학회지
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• 제13권1호
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• pp.31-42
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• 2019

Objectives: The purpose of this study is to analyze the relationship between the change gap in the perception of subjective hierarchy and medical expenditure and the factors influencing medical expenditure. Methods: An analysis based on the the data extracted from the Panel Study of Korea Health Panel for 2012-2013 (n=9,359) is conducted. Further in this study, data analysis included a chi-square test and logistic regression using SPSS version. 22.0 to analyze the factors influencing the turnover intention of industrial workers. Results: Model I showed decreases in medical expenditure by 1.247, 1.391, and 1.441 times in social classes one, two, and Model II showed an increase in medical expenditure by age, spouse, number of family members living together, insurance type, income class, economic activities, subjective health status, chronic illness and change on subjective recognition of social class. Conclusions: The study concludes that the state and community require psychological, social, and cultural support, in addition to individual efforts, to reduce medical expenditure.

• BMB Reports
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• 제55권10호
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• pp.494-499
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• 2022

PTEN-induced putative kinase 1 (PINK1) is a serine/threonine kinase that phosphorylates several substrates and exerts neuroprotective effects against stress-induced apoptotic cell death. Mutations in PINK1 have been linked to autosomal recessive forms of Parkinson's disease (PD). Mitophagy is a type of autophagy that selectively promotes mitochondrial turnover and prevents the accumulation of dysfunctional mitochondria to maintain cellular homeostasis. Toll-interacting protein (Tollip) was initially identified as a negative regulator of IL-1β receptor signaling, suppressing inflammatory TLR signaling cascades. Recently, Tollip has been reported to play a role in autophagy and is implicated in neurodegeneration. In this study, we determined whether Tollip was functionally linked to PINK1-mediated mitophagy. Our results demonstrated that Tollip promoted the mitochondrial processing of PINK1 and altered the localization of PINK1, predominantly to the cytosol. This action was attributed to increased binding of PINK1 to mitochondrial processing peptidase β (MPPβ) and the subsequent increase in MPPβ-mediated mitochondrial PINK1 cleavage. Furthermore, Tollip suppressed mitophagy following carbonyl cyanide m-chlorophenylhydrazone-induced mitochondrial dysfunction. These findings suggest that Tollip inhibits mitophagy via the PINK1/parkin pathway upon mitochondrial damage, leading to the blockade of PINK1-mediated neuroprotection.

• 국제학술발표논문집
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.1241-1241
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• 2022

In the current construction industry, difficulty arises in creating an adequate baseline schedule to establish a fundamental plan for construction. This presentation will present the research findings which investigated industry-recognized schedule metrics that aid in the successful implementation of said schedule. Industry organizations (Association for the Advancement of Cost Engineering, the Government Accountability Office, the Project Management Institute, and local city, state, and county government offices) provide standardized guidelines with specific metrics requirements to ensure successful implementation. However, most of those metrics are substantiated or validated in their effectiveness. The study examined the impact between these industry-recognized critical metrics and three distinct scheduling characteristics: Project Type, Project Duration, and Project Density (number of activities within a schedule). The research results showed that, among the 12 various schedules evaluated in parallel with 20 industry-recognized critical metrics, seven metrics substantially demonstrate a significant impact on a project schedule's success. Furthermore, six of the seven metrics directly correlate to at least one of the three scheduling characteristics outlined. As a result, this research has established more predictable outcomes based on impacts between three distinct project characteristics and 20 of the most discussed/researched critical scheduling metrics in the field. This allows management teams to have more confidence in establishing critical milestones and accurate turnover dates from the start of the project through its final completion.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1632-1637
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• 2006

We studied the changes in biochemical markers of bone metabolism in growing Thoroughbred horses. Serum osteocalcin (OC), as a marker for bone formation, and carboxy-terminal propeptide of type-I collagen (PICP), as a marker for bone formation, carboxy-terminal telopeptide of type-I collagen (ICTP), as a marker for bone resorption, were determined in nine clinically healthy horses from 3 d to 17 mo of age. The BW and withers height (WH) increased during the study. On the other hand, a rapid reduction in body weight gain (BWG) was observed between 1 mo and 9 mo of age and a rapid reduction in withers height gain was observed between 1 mo and 5 mo of age. The serum markers decreased significantly with increasing age. In particular, dramatic changes in serum markers occurred between 3 d to 1 wk and 5 to 7 mo of age in these horses, which suggests that bone turnover rapidly decreased after birth. On the other hand, the ratio of PICP to ICTP decreased through the experiment. This result suggests that the reduction in bone formation exceeded that of bone resorption. There was a significant correlation between markers and growth parameters, except for the correlation between PICP and BWG on single linear regression analysis. Serum OC and ICTP were affected by the WH in multiple linear regression analysis. These results indicated that the age-related variation in serum biochemical markers of bone metabolism reflected bone growth, but neither BW nor BWG. Therefore, we consider that changes in bone modeling are the major factor affecting the levels of serum biochemical markers by 17 mo of age in horses.

• 한국ITS학회 논문지
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• 제16권2호
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• pp.13-22
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• 2017

본 연구는 국도변에 설치되는 화물차휴게소의 이용수요를 예측하는 방법론을 제시하였다. 연구의 진행은 기존의 수요예측 방법론을 검토하여 이를 보완할 사항들을 제시하였다. 연구결과, 수요예측 과정에서 우선 할 사항은 휴게소를 이용할 화물차를 단기주차차량과 장기주차차량으로 구분하는 것이었다. 또한 단기와 장기 등 주차시간에 따라 소요주차면수 산정에 적용되는 이용률과 혼잡률 및 회전율에 차이가 발생하므로 이들 영향요소를 달리 적용할 것이 요구되었다. 그리고 화물차휴게소에 입주하는 업체와 편의시설의 활성화에 따라 서도 이용수요에 영향을 미치므로 이의 반영 필요성도 나타났다. 이로부터 국도변에 설치되는 거점형 화물차휴게소는 고속도로변에 설치되는 화물차전용 휴게소보다 수요예측 과정에 주의를 기울여야 되며, 또한 다양한 영향요소들을 감안할 필요성이 제기되었다.