• 대한의생명과학회지
• /
• 제26권4호
• /
• pp.360-367
• /
• 2020

Tuna skin byproduct extract (TSB) was used as a biocompatibility film base material, and its composite film with gellan gum (GG) was prepared. In addition, Pinus thunbergii cone extract (PTCE) was incorporated into the film to provide anti-oxidant and anti-bacteria activities. The tensile strength (TS) of the TSB/GG composite films increased with increasing GG content, whereas elongation at break (E) decreased. TSB/GG film at a ratio of 0.5:0.5 (w/w) showed the most desirable TS and E values. Based on scavenging free radical potentials and disc diffusion method results against growth of bacteria, antioxidant and anti-bacteria activities of films increased with increasing PTCE concentration. Accordingly, this study showed that TSB/GG could be used as a film material while the TSB/GG composite film containing PTCE can be utilized as functional packaging.

• 한국수산과학회지
• /
• 제51권4호
• /
• pp.376-382
• /
• 2018

We investigated the effect of replacing tuna byproduct meal (TBM) and rice bran (RB) with fish meal (FM) and macroalgae (MA) in extruded pellets (EP) supplied as a diet to juvenile Abalone Haliotis duscus in aquaculture. In total, 80,000 juvenile abalone were distributed among eight indoor raceways and supplied with one of four experimental diets. The control diet consisted of FM, fermented soybean meal, corn gluten meal and shrimp meal as protein sources, with wheat flour and dextrin as carbohydrate sources; the control diet also contained MA. In the FM50 diet, TBM was replaced with 50% FM. In the MA 50 diet, RB was replaced with 50% MA. The final diet, FM50+MA50, included TMB and RB in place of 50% FM and 50% MA. Abalone were fed to satiation with little food leftover for 16 weeks. Weight gain and specific growth rate of abalone fed the control diet were greater than those of abalone fed the FM50 and MA50 diets, but not different from those of abalone fed FM50+MA50 diet. The proximate composition of abalone soft body did not vary according to experimental diets. Based on these results, it appears that the traditional commercial diet for juvenile abalone, comprising FM and MA, could be replaced with one containing 50% TBM and 50% RB without any retardation of growth.

• 한국식품영양과학회지
• /
• 제46권4호
• /
• pp.465-472
• /
• 2017

본 연구에서는 고품질 인조육의 개발을 위하여 압출성형 인조육에 가공 부산물의 활용 측면에서도 장점을 지닌 참치 톱밥을 첨가하여 압출성형 공정을 거친 후 이화학적 특성에 대하여 살펴보았다. 압출성형 원료는 탈지대두분 65%에 분리대두단백 25%, 옥수수 전분 10%를 혼합한 것을 기본 배합시료로 설정하였고, 기본 배합 100%, 기본 배합에 참치 톱밥 15%를 첨가한 것과 참치 톱밥 30%를 첨가한 것, 세가지 시료를 제작하였다. 원료 사입량은 100 g/min, 스크루회전 속도 250 rpm, 사출구 온도 $140^{\circ}C$, 수분함량은 50%로 고정하여 압출성형한 후 비길이, 밀도, 파괴력, 조직잔사지수, 보수력, 조직감 분석, 수용성 질소 지수, 단백질 소화율, DPPH 라디칼 소거 활성을 실시하였다. 참치 톱밥의 첨가 함량이 높을수록 파괴력과 밀도는 감소하였고 단면 팽화율과 비길이는 증가하였다. 또한, 참치 톱밥 첨가 함량이 높을수록 조직잔사지수와 보수력은 감소하였으며 수용성 질소 지수와 단백질 소화율도 다소 감소하였다. DPPH 라디칼 소거 활성에서는 참치 톱밥의 첨가 함량이 높을수록 값이 증가하였으나 저장 기간이 30일과 60일로 길어짐에 따라 값이 감소하는 경향을 보였다. 산패도의 측정값은 참치 톱밥의 첨가 함량이 높을수록 감소하는 경향을 보였으나 60일 이후부터는 참치 톱밥의 첨가 함량이 높을수록 다소 증가하여 150일 이후에는 유의적 차이를 보였다. 기본 배합 시료에 참치 톱밥을 첨가하면 유연한 조직감과 영양적 가치를 보존하면서 항산화 기능이 증진되었다.

• Fisheries and Aquatic Sciences
• /
• 제25권6호
• /
• pp.335-349
• /
• 2022

To optimize the hydrolysis conditions in the production of antioxidant hydrolysates from tuna cooking juice concentrate (TC) to maximize the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, TC containing 48.91% protein was hydrolyzed with Alcalase 2.4 L, and response surface methodology (RSM) was applied. The optimum hydrolysis conditions included a 2.2% (w/v) Alcalase concentration and 281 min hydrolysis time, resulting in the highest DPPH radical scavenging activity of 66.49% (0.98 µmol Trolox/mg protein). The analysis of variance for RSM showed that hydrolysis time was an important factor that significantly affected the process (p < 0.05). The effects of different drying methods (freeze drying, hot air drying, and vacuum drying) on the DPPH radical scavenging activity and amino acid (AA) profiles of TC hydrolysate (TCH) were evaluated. Vacuum-dried TCH (VD) exhibited an increase in DPPH radical scavenging activity of 81.28% (1.20 µmol Trolox/mg protein). The VD samples were further fractionated by ultrafiltration. The AA profiles and antioxidant activities in terms of the DPPH radical scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power, and ferrous ion chelating activity were investigated. Glutamic acid, glycine, arginine, and cysteine were the major AAs found in the TCH fractions. The highest DPPH radical scavenging activity was found in the VD-1 fraction (< 5 kDa). The VD-3 fraction (> 10 kDa) exhibited the highest ABTS radical scavenging activity and ferric reducing antioxidant power. The ferrous ion chelating activity was the highest in VD-1 and VD-2 (5 to 10 kDa). In conclusion, this study provided the optimal conditions to obtain high antioxidant activities through TCH production, and these conditions could provide a basis for the future application of TCH as a functional food ingredient.

• Fisheries and Aquatic Sciences
• /
• 제19권3호
• /
• pp.14.1-14.10
• /
• 2016

Isoelectric solubilization/precipitation (ISP) processing allows selective, pH-induced water solubility of proteins with concurrent separation of lipids and removal of materials not intended for human consumption such as bone, scales, skin, etc. Recovered proteins retain functional properties and nutritional value. Four roe protein isolates (RPIs) from yellowfin tuna roe were prepared under different solubilization and precipitation condition (pH 11/4.5, pH 11/5.5, pH 12/4.5 and pH 12/5.5). RPIs contained 2.3-5.0 % moisture, 79.1-87.8 % protein, 5.6-7. 4 % lipid and 3.0-3.8 % ash. Protein content of RPI-1 and RPI-2 precipitated at pH 4.5 and 5.5 after alkaline solubilization at pH 11, was higher than those of RPI-3 and RPI-4 after alkaline solubilization at pH 12 (P < 0.05). Lipid content (5.6-7.4 %) of RPIs was lower than that of freeze-dried concentrate (10.6 %). And leucine and lysine of RPIs were the most abundant amino acids (8.8-9.4 and 8.5-8.9 g/100 g protein, respectively). S, Na, P, K as minerals were the major elements in RPIs. SDS-PAGE of RPIs showed bands at 100, 45, 25 and 15 K. Moisture and protein contents of process water as a 2'nd byproduct were 98.9-99.0 and 1.3-1.8 %, respectively. Therefore, yellowfin tuna roe isolate could be a promising source of valuable nutrients for human food and animal feeds.

• 한국수산과학회지
• /
• 제57권1호
• /
• pp.23-31
• /
• 2024

This study evaluated the utilization of a low fish meal (LFM) diet and black soldier fly (BSF) Hermetia illucens meal and oil as a fish meal (FM) substitute or functional additive for juvenile olive flounder Paralichthys olivaceus at the Jeju fish farm. Two experimental diets replaced FM using animal (tankage, poultry byproduct and tuna byproduct meal) and plant (wheat gluten and soy protein concentrate) protein sources, containing 45% (FM45) and 35% (FM35) of FM, respectively. One experimental diet replaced FM with animal, plant, and BSF meal, fish oil using insect meal and oil (FM35+). After the feeding trial ended, no differences in growth performance, feed utilization, survival and biological indices were observed among all experimental groups. Aspartate aminotransferase and cholesterol levels in the FM35 and FM35+ groups were significantly higher than that in the FM70 group. The linoleic acid level in the muscle was significantly higher in the fish fed with the FM70 diet than in those fed with the FM45, FM35, and FM35+ diets. Thus, the LFM diet is suitable for juvenile olive flounder farming during six months.