• KSBB Journal
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• 제11권5호
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• pp.518-523
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• 1996

효소 purine nucleoside phosphoryrase(NP)하 고 xanthine oxidase(XOD) 두 효소를 하나로 고정화시켜 산소 전극으로부터 ATPase 활성 계측용 바 이오 센서를 개발했다. 개발된 ATPase센서를 이용 해서 Thunnus albacares(Yeliowfin tuna), Tetra~ t turus audax(Striped marlin), Prognichthys agoo (Japanese flyingfish) 그리고 Cyprinus carpio ( ( Carp) 등의 각 4종의 어근육 단백질중의 $K^+-, Ca^{2}+- 그리고 Mg^{2+}-$ATPase 활성을 측정하였고, 시료 1 개를 측정는데 3분의 시간을 필요로 하였다. $K^+-, Ca^{2}+-$ATPase 활성의 경우 본 연구에셔 개발한 센 셔를 이용한 측정 결과와 종래(비색측정)법으로 측 정한 결과와의 사이에서 좋은 상관치를 얻었다.

• 한국수산과학회지
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• 제30권6호
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• pp.944-947
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• 1997

Docosahexaenoic acid (DHA) rich oil was obtained from blue fin tuna (Thunnus thynnus orientalis) orbital tissue with centrifugation of 12,000 rpm under vaccum $(10^{-1}\;Torr)\;at\;4^{\circ}C$. The effect of DHA rich oil (DHA content; $27.8\%$) on $CCl_4-induced$ acute injury was investigated biochemically and histopathologically. Dosage of DHA rich oil on 24h before $ CCl_4-administration$ prevented significantly the increase of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GTP) values. No necrosis of hepatocytes was observed in rat livers treated with DHA oil on 24h prior to $CCl_4-administration$. These results suggested that DHA oil controls the accumulation of fat in the liver and prevented the liver injury.

• Fisheries and Aquatic Sciences
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• 제21권1호
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• pp.1.1-1.12
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• 2018

Pacific bluefin tuna (Thunnus orientalis) is one of the commercially important species in Korea as well as other countries of the North Pacific. Korean offshore large purse seine fisheries targeting small pelagic fishes such as chub mackerel have caught T. orientalis temporarily in the east of Jeju Island. The catch of T. orientalis in March through June occupied approximately 60% of the total. The monthly catch around Jeju Island from 2004 to 2013 showed a negative correlation (r = - 0.755, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.856, p < 0.01) with the Pacific Decadal Oscillation Index (PDOI). The highest catch and catch per unit effort (CPUE) of T. orientalis around Jeju Island occurred either when the seawater temperature ranged between 15 and $16^{\circ}C$ at 50 m or when the catch was taken near the frontal area where two water masses from offshore and coastal areas collide. The length of T. orientalis caught around Jeju Island from 2004 to 2013 ranged from 19 to 193 cm in fork length (FL). The time series of the monthly mean FL of T. orientalis had a negative correlation (r = - 0.592, p < 0.01) with the seawater temperature at 50 m and had a significant positive correlation (r = 0.668, p < 0.05) with PDOI.

• 수산해양기술연구
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• 제54권2호
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• pp.116-123
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• 2018

Pacific bluefin tuna (Thunnus orientalis) has been mostly caught by the Korean offshore large purse seine fishery in Korean waters. The annual catch of Pacific bluefin tuna caught by the offshore large purse seine fishery in Korean waters showed less than 1,000 mt until the 1990s except for 1997. The catch sharply increased to 2,401 mt in 2000 and recorded the highest of 2,601 mt in 2003, but the catch has generally decreased with a fluctuation thereafter. The main fishing ground of Pacific bluefin tuna of this fishery is formed around Jeju Island. However, it expanded to the Yellow Sea, the coastal of Busan, and the East Sea, which depends on the migration patterns of Pacific bluefin tuna by season. The CPUE standardization of Pacific bluefin tuna was conducted using Generalized Linear Model (GLM) to assess the proxy of the abundance index. The data used for the GLM were catch (weight), effort (number of hauls), catch ratio of Pacific bluefin tuna, moon phase by year, quarter and area. The standardized CPUE from 2004 to 2011, except for 2003 and 2010, showed a steady trend, and then increased until 2014. The CPUE in 2015 decreased, and in 2016 was higher than that in 2015. The result of GLM suggests that the effect of the catch ratio of Pacific bluefin tuna is the largest factor affecting the nominal CPUE.

• 한국수산과학회지
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• 제43권6호
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• pp.740-746
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• 2010

This study standardized the catch per unit effort (CPUE) of bigeye tuna (Thunnus obesus) caught by the Korean longline fishery in the Pacific Ocean. The study used a general linear model (GLM) to standardize the CPUE using catch and effort data aggregated by year, month, and $5{\times}5$ degrees of latitude and longitude in the tropical Pacific Ocean from 1982 to 2008 (except for 1988 and 1989). Explanatory variables in the GLM analysis included year, month, subtropical area, and number of hooks between floats (HBF). The study area and HBF were subdivided into three subtropical areas and six classes, respectively. During the study period, the standardized CPUE was significantly related to the nominal CPUE. The standardized CPUE declined sharply, as compared to the nominal CPUE, after the mid 1990s. In particular, the decline in the standardized CPUE in all subtropical areas in the 2000s was clearer than that of the nominal CPUE.

• 수산해양기술연구
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• 제59권2호
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• pp.125-134
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• 2023

The swimming behavior of pacific bluefin tuna (Thunnus orientalis) in the offshore sea cage of the brass fishing net was observed and analyzed by imaging sonar techniques. The cultured fish spent most of the time swimming a circular path along the circular cage wall and continued to swim only clockwise direction without completely changing the swimming direction during the 23-hour observation time. In addition, changed swimming behaviors were divided into four categories: (a) the behavior of a large group temporarily swimming in the opposite (counter clockwise) direction, (b) the behavior of a small group temporarily swimming in a small circular path, (c) the behavior swimming small circular path in the center of the cage, and (d) the behavior of a large group swimming across the center of the cage. The maximum swimming speed of the cultured fish was from 3.5 to 3.8 TL/s, the mode was from 1.2 to 1.4 TL/s and the swimming speed during the day time was faster than at night time. It was confirmed the cultured fish swam not only on the surface but also near the bottom net of the cage during the day, but swam mainly at the upper part of the cage at night.

• 한국식품영양과학회지
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• 제22권4호
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• pp.448-457
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• 1993

혈합육어 중 연근해 온대산 멸치의 내장과 고등어의 유문수, 그리고 원양 열대산 황다랭이 및 날개다랭이의 유문수를 각각 시료로 하여 trypsin을 분리 정제하였으며, 그 분자량과 아미노산 조성 및 효소의 반응 최적조건에 관하여 비교 분석하였다. 실험결과를 요약하면 다음과 같다. 1. 멸치의 내장과 고등어, 황다랭이 및 날개다랭이의 유문수에서 각각 황산암모늄염석, benzamidine-Sepharose 6B 친화성 크로마토그래피, DEAE-Sephadex A-50 크로마토그래피, Sephadex G-75 겔 여과 크로마토그래피 등의 방법을 혼용하여 고등어로부터는 2종의 trypsin (고등어 trypsin A와 고등어 trypsin B로 명명함)을, 그리고 다른 어류로부터는 각각 1종의 trypsin을 분리 정제하였다. 2. 멸치 trypsin과 고등어 trypsin B는 고등어 trypsin A와 황다랭이 trypsin 및 날개다랭이 trypsin에 비하여 그 고유활성이 월등히 높았다. 3. 이들 혈합육어 trypsin의 분자량은 22kDa-26kDa 범위였다. 4. 이들 trypsin은 공통적으로 glycine, serine, aspartic acid를 많이 함유하고 있었으며, tryptophan, methionine, lysine, tyrosine의 함유량은 적었다. 5. BA-$\rho$-NA기질에 대한 반응 최적조건은 멸치 trypsin은 pH 8.0에서 45$^{\circ}C$, 고등어 trypsin A와 B는 pH 8.0에서 5$0^{\circ}C$, 그리고 황다랭이 trypsin은 PH 9.0에서 55$^{\circ}C$, 날개다랭이 trypsin은 pH 9.0에서 5$0^{\circ}C$였다. 6. 위에 든 실험 결과들은 혈합육 어류의 서식온도가 이들 어류의 trypsin의 반응 최적 온도와는 어느 정도 관계가 있을 것으로 추측되었다.

• 수산해양기술연구
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• 제27권4호
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• pp.225-231
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• 1991

본 연구는 눈다랭이류 위주의 조업을 위한 어장선정의 자료를 제시하고자 태평양, 대서양 및 인도양의 다랭이 연승어업 어획통계자료를 이용하여 데이터베이스를 작성하였다. 사용된 자료는 국립수산진흥원에서 1975년부터 1987년까지 발행한 13년간의 한국 원양 다랭이 연승어업 어획통계자료 및 어장도이며, 데이터베이스는 16비트 개인용컴퓨터를 사용하여 시스템을 구성하였다. 결과를 요약하면 다음과 같다. 1) 본 데이터베이스 프로그램을 기동한 후 메뉴화면의 요청에 따라 대양과 데이터형을 선택하면, 먼저 대양의 지도가 칼라모니터에 그려지고, 경위도 5$^{\circ}$구획의 각 해구에 투입낚시수, 조획율 및 눈다랭이 조획비율 등이 표시되므로 어장선정을 위한 판단자료를 동시에 검색할 수 있다. 2) 조획율 예측은 어느 지정된 해역의 월별변동의 자료로부터 최소자승법에 의해 지정된 월의 예측조획율을 구하고, 연도별변동의 자료로부터 지정된 년의 예측조획율을 구해서 이들 둘을 산술평균해서 구하였다. 실제와 예측치를 비교해 본 결과 예측결과가 실제에 비교적 근접되어 나타나서 본 데이터베이스에 의한 예측이 가능한 것으로 판단되었다. 3) 눈다랭이의 어획비율, 어가 및 어체중량에서 볼 때 앞으로 눈다랭이 위주로의 심층조업이 연승어업의 경영개선에 도움이 될 것으로 판단된다.

• 한국수산과학회지
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• 제56권4호
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• pp.363-372
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• 2023

This study was developed to prepare hamburger steak (HS), wantang soup (WS), and tomato soup (TS) for senior-friendly seafood's (SFS) using bigeye tuna Thunnus obesus, and to investigate their quality characteristics. All data were presented in the order of HS, WS, and TS. The hardness values were 298.9×1.0³, 47.1×1.0³ and 14.9 ×1.0³ N/m², respectively. The viscosity of TS was 2,856 mPa·s. The protein contents were 13.8, 11.7, and 9.7 g/100 g, respectively. The fat-soluble vitamins content were as follows: vitamin A 21.88, 5.03, and 23.72 ㎍RAE/100 g; vitamin D 1.15, ND, and 1.81 ㎍/100 g, respectively. The water soluble vitamins contents were as follows: vitamin C 47.22, 32.83 and 37.01 mg/100 g; vitamin B₂ 0.17, 0.11 and 0.10 mg/100 g; vitamin B₃ 34.87, 34.76 and 54.62 mgNE/100 g. The Ca contents were 15.9, 16.8, 28.9 mg/100 g, and the K contents were 383.8, 167.4, and 300.0 mg/100 g, respectively. The dietary fiber was 0.04, 0.07 and 0.08 g/100 g, respectively. Escherichia coli was not detected in any of the products. These results suggest that the products should be classified as follows, based on their appropriateness as SFS: HS, WS and TS.

• Fisheries and Aquatic Sciences
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• 제5권3호
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• pp.206-211
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• 2002

The aim of this study was to obtain the basic data on the fish school behavior change to approaching vessel and fish species identification by means of their swimming speed. The surveys were carried out for the juvenile southern blue fin tuna and other fish schools off Esperance, western Australia from January to March 1999. We observed changes of fish school behavior in response to the approaching vessel using 360-degree scanning sonar. The results showed that, a horizontal direction index used to quantify a change of fish school behavior did not identify dependence of a radial distance and a swimming speed. A Mann­Whitney test conducted using the horizontal swimming speed of both species identified by sonar specialists, did not reveal a significant difference.