• 제목/요약/키워드: Tubular Cell

검색결과 291건 처리시간 0.033초

Ni-Fe/YSZ 코어-쉘 구조 연료극을 사용한 다전지식 고체산화물 연료전지의 전기화학적 특성 (Electrochemical Properties of Segmented-in-series SOFC Using Ni-Fe/YSZ Core-shell Anode)

  • 안용태;지미정;황해진;이민진;홍선기;강영진;최병현
    • 한국세라믹학회지
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    • 제51권4호
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    • pp.357-361
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    • 2014
  • An Ni-Fe/YSZ core-shell structured anode for uniform microstructure and catalytic activity was synthesized. Flat tubular segmented-in-series solid oxide fuel cell-stacks were prepared by decalcomania method using synthesized anode powder. The Ni-Fe/YSZ core-shell anode exhibited better electrical conductivity than a commercially available Ni-YSZ cermet anode. Also power output increased by 1.3 times with a higher open circuit voltage. These results can be attributed to the uniformly distributed Ni particles in the YSZ framework. The impedance spectra of a Ni-Fe/YSZ core-shell anode showed comparable reduced ohmic resistance similar to those of the commercially available Ni-YSZ cermet anodes.

韓國産 冬眠 박쥐의 精巢와 副精巢의 週期的인 變化에 관한 硏究 (Periodic Changes of the Testis and Ductus Epididymis in Korean Hibernating Bats)

  • Oh, Yung-Keun
    • 한국동물학회지
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    • 제20권2호
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    • pp.67-76
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    • 1977
  • The periodic changes of testis and \ulcorner션 epididymidis in Korean hibernating bats, the oriental discoloured bats (Vesportilio superans Thomas) and the Korean greater horseshoe bats (Rhinolophus ferrumequinum korak Kuroda) were studied in order to clarify the possibility of correlation between their histological findings and one of physiological facets, hibernation, and the results obtained were as follows: 1. The spermatogenic function of the oriental discoloured bats obtained in July and August was depressed although the spermatocytes showed a considerable cell proliferation. Few mature sperms were observed in the seminiferous tubules of the bat obtained in August. 2. The spermatogenic function of the Korean greater horeshoe bats obtained in September was not remalkable but a considerable number of sperms were stored in the excretory ducts which were characterized by existence of para-tubular spaces in the ductus epididymidis. 3. The spermatogenic epithelia of the Korean greater horseshoe bats obtained in December showed histologically atrophied figures. However, a vast number of sperm remained in extremely expaned luminae of the ductuc epididymidis which epithelial cells were maintained rectangular in shape. 4. These results suggest that there are periodic changes of the spermatogenic epithelia and the excretory ducts, and that those histological changes are closely related to their wintering.

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Paecilomyces japonica의 반복투여가 랫드에 미치는 영향 (Effect of repeated Paecilomyces japonica treatment on rats)

  • 김용범;홍다해;조은상;임완중;김일환;손화영
    • 대한수의학회지
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    • 제54권1호
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    • pp.31-38
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    • 2014
  • Cordyceps is a fungus used as a traditional medicine in China, Japan, and Korea. Paecilomyces (P.) japonica is a new cordyceps that was recently cultivated on silkworm pupae in Korea. The present study evaluated the toxicological effects of P. japonica in rats. Forty rats were treated with oral doses of P. japonica (0, 20, 100, or 500 mg/kg/day) for 4 weeks. Twenty additional rats were treated with 0 or 500 mg/kg/day of P. japonica for 4 weeks and then maintained for 2 weeks without treatment. Clinical signs, body weight, food and water consumption, and organ weight as well as hematology, serum biochemistry, and histopathology data were examined. Body weight gain of the group treated with 500 mg/kg/day was significantly reduced. Microscopically, karyomegaly, single cell necrosis, and mitosis were observed in the renal tubular epithelium of all treated groups. In conclusion, P. japonica caused a reduction of body weight and renal injury in rats. The no observed adverse effect level (NOAEL) of P. japonica was less than 20 mg/kg/day.

Spermatogenic Effect of Sulfur and Methyl Sulfonyl Methane in Rats

  • Lee, Young-Lae;Park, Jeong-Sook;Hwang, Seock-Yeon;Han, Kun
    • Natural Product Sciences
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    • 제18권3호
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    • pp.204-210
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    • 2012
  • Sulfur has been used as a general physical strengthening agent from ancient times in Asia. On the basis of this point, we measured spermatogenic effect of sulfur and methyl sulfonyl methane (MSM) which is used a functional food for arthritis in rats. MSM, a kind of organic sulfur, is used as an alternative of sulfur by some people. Sulfur was administered as a dietary supplement and MSM was administered orally as a solution to 7 week old rat for 6 weeks. All sulfur administered groups showed a significant dose dependent increase in the number of sperm in the testes compared with the control group. Moreover, a histological examination showed an apparent increase in the number of seminiferous tubular cell layers in the testes of the sulfur treated rats. However, there were no observed any increase of sperm in MSM dosing group. In sulfur treated rats, the weights of body, liver, spleen, kidney, testes and epididymides didn't show significantly differences compared with the control. Histopathological examination was not revealed any morphological change in the liver, spleen and kidney. Thus, sulfur may be effectively used to treat sperm deficiency of men, but not MSM.

In Vitro Cytotoxicity of a Novel Platinum(II) Coordination Complex Containing Diaminocyclohexane

  • Jung, Jee-Chang;Kim, Soon-Ae;Kim, Young-Kyu;Chang, Sung-Goo;Rho, Young-Soo
    • Biomolecules & Therapeutics
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    • 제8권3호
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    • pp.228-234
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    • 2000
  • We have synthesized a novel platinum(II) coordination complex containing trans-ι-1,2-diaminocy-clohexane (DACH) as a carrier ligand and 1,2-dichloroethane (DCE) as a leaving group. A new series of [Pt(trans-ι-DACH)(DCE)](PC) was evaluated for its cytotoxic activity on MKN-45 human gastric adenocar-cinoma cells and normal primary cultured kidney cells. The new platinum complex has demonstrated high efficacy in the cytotoxicity against MKN-45/P, MKN-45/ADM and MKN-45/CDDP cell-lines. The cytotoxicity of PC against rabbit proximal renal tubular cells, human renal cortical cells and human renal cortical tissues, determined by MTT assay, the [$^3H$]-thymidine uptake arid glucose consumption tests, was found to be quite less than those of cisplatin. Based on these results, this novel platinum(II) coordination complex appears to be better for improving antitumor activities with low nephrotoxicity and is a valuable lead in the development of new, clinically available anticancer chemotherapeutic agents.

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DA-l131/betamipron의 랫드에 대한 4주반복 정맥투여 독성시험 (A Four-Week Intravenous Toxicity Study of DA-l131/betamipron in Rats)

  • 김동환;조현;강경구;백남기;김원배
    • Biomolecules & Therapeutics
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    • 제6권2호
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    • pp.204-212
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    • 1998
  • This study was conducted to evaluate the repeated dose toxicity of DA-1131/betamipron, newly developed carbapenem antibiotic, in rats. DA-1131/ betamipron was administered intravenously once a day for 4 weeks to 10 males and 10 females per group at the doses of 0(control),40, 160 and 640 mg/kg. Throughout the study period, all rats survived. The administration of DA-1131/betamipron induced soft stool or diarrhea in rats of both sexes receiving 160 or 640 mg/kg. The water consumption was increased with a statistical significance in 640 mg/kg during observation period. At the end of administration, hematological and serum biochemical examination showed no toxicological changes in DA-1131/betamipron treated groups compared with control group. Histopathologic examination revealed inflammatory cell infiltration, tubular dilatation and focal necrosis of kidney in two males and three females in 640 mg/kg. On the basis of these results, the noobserved-adverse-effect-level of DA-1131/betamipron was estimated to be 40 mgtg under the present test condition.

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1,2-비스 (디페닐포스피노)에탄을 배위자로 한 항암성백금 (II) 착체의 위암세포와 정상신장세포에 대한 선택적 세포독성 (Selective Cytotoxicity of Pt (II) Complex Containing 1,2-Bis (diphenylphosphino)ethane on Human Gastric Cancer Cell-Lines and Normal Kidney Cells)

  • 노영수;장성구;정지창
    • 약학회지
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    • 제44권5호
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    • pp.399-405
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    • 2000
  • We have synthesized a novel platinum (II) coordination complex containing trans-ι-1,2-diaminocyclohexane (DACH) as a carrier ligand and 1,2-bis (diphenylphosphino)ethane (DPPE) as a leaving group. In addition, nitrate was added to improve the water-solubility. A new series of [Pt (trans-ι-DACH) (DPPE)].2NO$_3$(PC) was evaluated for its cytotoxic activity on MKN-45 human gastric adenocarcinoma cells and normal primary cultured kidney cells. PC has demonstrated high levels of cytotoxicity against MKN-45/S, MKN-45/ADR and MKN-45/CDDP cells. The cytotoxicity of PC against rabbit proximal renal tubular cells, human renal cortical cells, and human renal cortical tissues, determined using the MTT assaying technique, the ($^3$H)-thymidine uptake and the glucose consumption tests, was found to be quite less than those of cisplatin. Based on these results, this novel platinum (II) coordination complex appears to be better for improving antitumor activities with low nephrotoxicity and is a valuable lead in the development of new clinically available anticancer chemotherapeutic agents.

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Effects of Albizia julibrissin Durazz through Suppression of Mitochondrial Fission and Apoptosis in Cisplatin-induced Acute Kidney Injury

  • Hui-Ju Lee;Kyung-Hyun Kim;Yae-Ji Kim;Sung-Pil Cho;Geum-Lan Hong;Ju-Young Jung
    • Natural Product Sciences
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    • 제28권4호
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    • pp.194-200
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    • 2022
  • Albizia julibrissin Durazz. (AJ; family Minosaceae) is widely distributed worldwide, and its stem bark has been used as a traditional herbal medicine. Acute kidney injury (AKI) is a clinical syndrome that results in sudden loss of renal function. This study aimed to investigate the effects of AJ against cisplatin-induced AKI using a human kidney proximal tubule epithelial cell line (HK-2) and cisplatin-treated mice. In vitro, cisplatin treatment increased apoptosis in HK-2 cells. However, AJ treatment decreased apoptosis of cisplatin-treated HK-2 cells. In vivo, cisplatin treatment accelerated renal injury by increasing the levels of renal injury markers, such as blood urea nitrogen, creatinine, kidney injury molecule 1, and neutrophil gelatinase-associated lipocalin, which were reversed by AJ treatment. Histopathologically, AJ treatment resulted in decreased renal damage with less tubular necrosis and brush border desquamation compared with the AKI group. Additionally, cisplatin treatment upregulated mitochondrial fission, a pathological characteristic of AKI, which was downregulated by AJ treatment. Along with increased mitochondrial fission, AJ treatment also reduced cisplatin-induced apoptosis. These results suggest that AJ may be a potential therapeutic agent for cisplatin-induced AKI.

Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans

  • Yan Sun;Seung-Min Lee;Bon-Jin Ku;Myung-Jin Moon
    • Applied Microscopy
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    • 제50권
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    • pp.20.1-20.9
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    • 2020
  • Arthropods have an open circulatory system with a simple tubular heart, so it has been estimated that the contractile pumping structure of the cardiac muscle will be less efficient than that of vertebrates. Nevertheless, certain arthropods are known to have far superior properties and characteristics than vertebrates, so we investigated the fine structural features of intercalated discs and cardiac junctions of cardiac muscle cells in the black widow spider Latrodectus mactans. Characteristically, the spider cardiac muscle has typical striated features and represents a functional syncytium that supports multiple connections to adjacent cells by intercalated discs. Histologically, the boundary lamina of each sarcolemma connects to the basement membrane to form an elastic sheath, and the extracellular matrix allows the cells to be anchored to other tissues. Since the intercalated disc is also part of sarcolemma, it contains gap junctions for depolarization and desmosomes that keep the fibers together during cardiac muscle contraction. Furthermore, fascia adherens and macula adherens (desmosomes) were also identified as cell junctions in both sarcolemma and intercalated discs. To enable the coordinated heartbeat of the cardiac muscle, the muscle fibers have neuronal innervations by multiple axons from the motor ganglion.

Johannes Nathanael Lieberkühn (1711-1756): luminary eighteenth century anatomist and his illuminating discovery of intestinal glands

  • Sanjib Kumar Ghosh
    • Anatomy and Cell Biology
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    • 제56권1호
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    • pp.25-31
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    • 2023
  • Johannes Nathanael Lieberkühn was a prodigious anatomist whose meticulous experiments and precise detailing helped in comprehending the microscopic anatomy of digestive system during early part of eighteenth century. Notably, his inventions in the field of microscopy aptly complemented his quest for anatomical knowledge at microscopic level. He designed a reflector (Lieberkühn reflector) which enhanced the amount of focussed light leading to bright illumination of tissue specimen. He invented the solar microscope which provided excellent resolution of minute anatomical details. Lieberkühn discovered the digestive juice secreting tubular glands (glands of Lieberkühn) present at the base of intestinal villi producing epithelial invaginations (crypts of Lieberkühn). He also described the intricate juxtaposition of blood vessels in relation to a single intestinal villi. Moreover, through empirically designed experimental set up, Lieberkühn was able to demonstrate the flow of lymph from intestinal villi to collecting lymphatic vessels. Also, his grandiose collection of laboratory specimens involving vascular anatomy are a testimony of his untiring efforts in academia. His contributions were seminal in comprehending the anatomy of digestive system and paved the way for future revelations. His work unveiled the enormous scope of microanatomy in medical science and catalysed the advent of histological staining methods a century later.