• Korean Journal of Agricultural Science
• /
• v.16 no.1
• /
• pp.26-35
• /
• 1989

Studies were carried out to compare from the viewpoint of peroxidase isoenzyme patterns by ages, locations and organs. In addition, the relationships among six cultivars peroxidase isoenzyme patterns were compared and the relationships between cultivars and their hybrids were examined. The results are summarized as follows. 1. The zymogram was not affected by the ages of 'Fuji' cultivar. 2. There was no locational difference in zymogram among Suwon, Taejon and Yesan in 'Fuji' and 'Tsugaru' cultivars. 3. Significant difference in isozyme patterns was found between stems and leaves in 'Fuji' and 'Tsugaru' cultivars. 4. There were some differences in zymogram among 'Ralls Genet', 'Fuji', 'Jonathan', 'Starking', 'Golden Delicous' and 'Tsugaru' cultivars. 5. Classification was made by the peroxidase isozyme development between cultivars and their hybrids. 1) The type showed both parent's isozyme pattern. 2) The type showed a loss of a part of parent's isozyme bands. 3) The type developed new isozymes which were not detected in parent plants.

• Journal of Life Science
• /
• v.16 no.5
• /
• pp.834-839
• /
• 2006

This study was carried out to investigate changes in cell wall components and solubilization and depolymerization of pectin and neutral sugar polymers during softening of 'Tsugaru' apples. Pectic polysaccharides were solubilized in different solvents, distilled-water, 0.05 M CDTA, 0.05 M $Na_2CO_3$, and 8 M KOH, from cell wall materials during fruit softening. The uronic acid contents in distilled-water fraction rapidly increased along with fruit softening at 4 weeks after ambient storage. In the change of non-cellulosic neutral sugars in the cell wall of ‘Tsugaru’ fruits, the major sugar was galactose and arabinose in distilled-water, 0.05 M CDTA and 0.05 M $Na_2CO_3$ soluble fractions, and it was glucose, galactose and xylose in 8 M KOH fraction. Especially the change of galactose contents in distilled-water fraction was increased greatly along with fruit softening. When uronic acid polymers (UAP) and carbohydrate polymers (CP) in distilled-water fraction were filtered and separated using Sepharose CL-2B column, the high molecular UAP and CP were degraded to the low molecular ones from at harvest to softening fruit. Thus, the amount of high molecular polymers were greatly decreased along with fruit softening.

• Horticultural Science & Technology
• /
• v.30 no.6
• /
• pp.776-779
• /
• 2012

A new cultivar 'Yeohong' was originated from an artificial cross between 'Jonathan' and 'Fuji' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-31', it was finally selected in 2007. 'Yeohong' is characterized by the following. Optimum harvest time is late September. Shape of mature fruit at 278 g on mean weight is oblate a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $14.3^{\circ}Brix$ and titratable acidity at 0.4%, which results in a gustatory harmony between sugars and acids. Safe storage period for the fruit is 3 weeks at room temperature. It is not resistant to Bitter rot. 'Yeohong' also reveals a physiological cross compatibility with leading cultivars such as 'Hongro' and 'Tsugaru', but a physiological cross incompatibility with 'Fuji'. It has weak growth habit.

• Horticultural Science & Technology
• /
• v.29 no.6
• /
• pp.655-658
• /
• 2011

A new cultivar 'Hongan' was originated from an artificial cross between 'Fuji' and 'Jonathan' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-32', it was finally selected in 2006. 'Hongan' is characterized by the following. Optimum harvest time is late September. Shape of the mature fruit at 309 g on mean weight is globosity with a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $13.7^{\circ}Brix$ and an acidity at 0.28%. Safe storage period for the fruit is 3 weeks at room temperature. It is resistant to Bitter rot. 'Hongan' also reveals a physiological cross compatibility with leading cultivars such as 'Gamhong' and 'Tsugaru' in addition to its maternal parent 'Fuji'. Tree topology is semi-spreading with vigorous growth habit.

• Horticultural Science & Technology
• /
• v.33 no.5
• /
• pp.784-788
• /
• 2015

A new cultivar 'Picnic' originated from an artificial cross between 'Fuji' and 'Sansa' at National Institute of Horticultural & Herbal Science in 1994. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2003. After regional adaptability tests in five districts for four subsequent years as 'Wonkyo Ga-34', it was ultimately selected in 2008. Optimum harvest time is late September. Mature fruit has mean weight of 233 g and is conic with light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains a favorable total soluble solids content at $13.8^{\circ}Brix$ and titratable acidity at 0.43%, which results in gustatory harmony between sugars and acids. It is not resistant to bitter rot or Marssonina blotch. 'Picnic' exhibits a physiological cross compatibility with leading cultivars such as 'Fuji', 'Hongro' and 'Tsugaru'. Tree topology is semi-spreading with a weak growth habit.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.556-559
• /
• 2009

"Hongso" was originated from a cross between "Yoko" and "Hongro" in NIHHS, RDA, in 1989. "Hongso" was preliminarily selected in 2002 for its high fruit quality. After regional adaptability test at five sites from 2003 to 2006 as "Wonkyo Ga-30" it was finally selected in 2006. Optimum harvest time is early September which is similar to that of "Hongro". Fruit shape is globose conical and skin color is light red. Mean fruit weight is 295g and soluble solids content is 14.1oBrix. Fruits acidity is 0.34%, which is higher than that of "Hongro"(0.23%). It has a good taste for harmony of sugar and acidity. Storability is 3 weeks in room temperature. It is apt to russet on fruit skin. It is susceptible to Bitter rot. "Hongso" has a good cross compatibility with major cultivars such as "Fuji", "Hongro" and "Tsugaru". Tree habit is semi-spreading and tree vigor is weak. "Hongso" is high-density cultivation type cultivar, because it has a precocity, spur-type.

• Korean Journal of Agricultural Science
• /
• v.25 no.1
• /
• pp.12-18
• /
• 1998

This study was conducted to examine the effect of several postharvest treatments on the storable quality of three apple cultivars, 'Tsugaru', 'jonagold', and 'Hokudo' which are difference in harvest season. Ethylene evolution level showed cultivar-dependent pattern during storage and there was no correlation between the amount of ethylene evolution and storage period among cultivars. Polyethylene film(PE) wrapping was effective in the inhibition of water loss and functional ceramic film was also effective in the prevention of ethylene accumulation inside the wrap during 1 to 3 months of cold storage compared to those of simple PE wrapping. Ethylene scrubber and wax treatment positively contributed to keep the fruit firmness 10% higher than that of control fruit during storage. Waxing enhanced the appearance of 'jonagold' even after simulated marketing period through inhibiting wax accumulation on the fruit surface.

• Journal of Applied Biological Chemistry
• /
• v.43 no.3
• /
• pp.170-175
• /
• 2000

A pot-lysimeter experiment was conducted with 3-year-old 'Tsugaru' apple (Malus domestica Borkh) trees to examine the changes in oxygen diffusion rate (ODR) with lateral flow velocity of water through soil. The influence of lateral water flow velocity on water relations and elemental content in leaf, and sap temperature distribution patterns of the xylem of trees were also determined. Trees were grown under four soil water regimes: (1) fast laterally flowing (FWT, $2.50{\times}10^{-4}cm\;s^{-1}$), (2) slow laterally flowing (SWT, $0.25{\times}10^{-4}cm\;s^{-1}$), and (3) stagnant water table (WLT) at 60-cm, and (4) drip-irrigation at -40 kPa of soil matric potential as a control. The rate of $O_2$ diffusion converged near $2{\times}10^{-3}g\;m^{-2}\;min^{-1}$ for FWT and control soils, but decreased below $1{\times}10^{-3}g\;m^{-2}\;min^{-1}$ 40 days after treatment (DAT) for WLT soils. For SWT soils, however, the ODR at 15 cm below the soil surface was similar to that of control, but at 45 cm below the soil surface, ODR was similar to that of the WLT treatment. Leaf water potential of FWT and SWT plants was similar to that of control plants, but the values for SWT plants declined by 98 DAT. Leaf water potential of WLT plants decreased from -1.86 MPa (9 DAT) to -2.41 MPa (59 DAT) and finally down to -2.70 MPa. The sap temperature measured at 1100-hr was lowest at top and highest at bottom for FWT and control plants, but this pattern of SWT and WLT plants was disturbed from 29 DAT. However, for SWT plants, such thermal disturbance of sap temperature disappeared from 63 DAT.

• Horticultural Science & Technology
• /
• v.17 no.3
• /
• pp.337-340
• /
• 1999

In order to evaluate stored apple pollen viability, in vitro germination test was performed on a microscope slide coated with the culture medium containing fluorescein diacetate (FDA). However, the inclusion of FDA to the culture medium declined pollen germination. Alternatively, the fluorochromatic reaction procedure was tested. The procedure involved dusting pollen grains onto drops of 10% sucrose solution containing 0.002% FDA and allowing them to accumulate fluorescein. Within 30 min after the fluorochromatic reaction, viable pollen grains clearly fluoresced under ultraviolet light. Both the in vitro germination test and the fluorochromatic reaction procedure revealed that stored apple pollen viability was not considerably decreased over storage up to at least 39 months. Of the cultivars examined by both methods, 'Fuji' and 'Senshu' pollen viability was highest, 'Tsugaru' was intermediate, and 'Jonagold' was lowest. The fluorescing percentages appeared approximately comparable to the germination percentages except for the 'Senshu' pollens stored for 3 months, although the fluorescing percentages was slightly higher than the germination percentages. Strong and highly significant correlations were found between the two methods. It can thus be concluded that the fluorochromatic reaction procedure provides a convenient and reliable evaluation of stored apple pollen viability.

• Korean Journal of Plant Resources
• /
• v.28 no.5
• /
• pp.641-647
• /
• 2015

This study was conducted to select native ground cover plants for sod culture in an organic apple orchard by estimating the effect of three native ground cover plants, Glechoma hederacea, Thymus magnus, and Ixeris stolonifera, on the soil coverage, time-periodic weed occurrence, fruit characteristics, and soil chemical properties. The plant height of G. hederace, T. magnus and I. stolonifera were 15.0 ㎝, 13.4 ㎝ and 7.2 ㎝, respectively. The dry weight of G. hederace, T. magnus and I. stolonifera were 463 ㎏/10a, 247 ㎏/10a, and 255 ㎏/10a, respectively. The plant height and dry weight of G. hederacea were higher than in the other species. T. magnus and I. stolonifera having relatively lower soil cover rate during their life cycle produced a lot of weeds in the orchard as compared with the control. In contrast, G. hederacea showed 100 percentage of ground cover in the first year, and maintained high percentage of ground cover in the growing season of ‘Tsugaru’ apple for another 2 years. When the soil was covered with G. hederacea in the orchard for 3 years, the amount of weed was only 114 ㎏/10a and number of weeding was also reduced about 33% compared with control as well as the other species. There were no differences in the tree growth and fruit characteristics between the native ground cover plants and the control; however, positive effects of native ground cover plants on soil chemical properties were found. In G. hederacea, available P₂O₄content in soils remarkably increased and was a significant difference among native species. In addition, cation (Ca, Mg and K) content in soils increased by 39% in Ca, 6% in Mg, and 11% in K at G. hederacea compared with control. These results suggest that G. hederacea could be advantageous in terms of reducing the amount of herbicide applied and the labor required for weed control, and controlling soil chemical properties; therefore, it is a good candidate for sod culture in an organic apple orchard.