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http://dx.doi.org/10.5352/JLS.2006.16.5.834

Changes in Cell Wall Components, and Solubilization and Depolymerization of Pectin and Neutral Sugar Polymers during Softening of 'Tsugaru' Apples  

Choi, Cheol (Division of Plant Biosciences, Department of Agriculture and Life Sciences, Kyungpook National Univ.)
Kang, In-Kyu (Dept. Environmental Horticulture, Sangju Netional Univ.)
Publication Information
Journal of Life Science / v.16, no.5, 2006 , pp. 834-839 More about this Journal
Abstract
This study was carried out to investigate changes in cell wall components and solubilization and depolymerization of pectin and neutral sugar polymers during softening of 'Tsugaru' apples. Pectic polysaccharides were solubilized in different solvents, distilled-water, 0.05 M CDTA, 0.05 M $Na_2CO_3$, and 8 M KOH, from cell wall materials during fruit softening. The uronic acid contents in distilled-water fraction rapidly increased along with fruit softening at 4 weeks after ambient storage. In the change of non-cellulosic neutral sugars in the cell wall of ‘Tsugaru’ fruits, the major sugar was galactose and arabinose in distilled-water, 0.05 M CDTA and 0.05 M $Na_2CO_3$ soluble fractions, and it was glucose, galactose and xylose in 8 M KOH fraction. Especially the change of galactose contents in distilled-water fraction was increased greatly along with fruit softening. When uronic acid polymers (UAP) and carbohydrate polymers (CP) in distilled-water fraction were filtered and separated using Sepharose CL-2B column, the high molecular UAP and CP were degraded to the low molecular ones from at harvest to softening fruit. Thus, the amount of high molecular polymers were greatly decreased along with fruit softening.
Keywords
Apple; cell wall; uronic acid; non-cellulosic neutral sugar; fruit softening;
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