• 미생물학회지
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• 제35권2호
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• pp.158-163
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• 1999

제주도 밀감 과수원의 토양으로부터 polygalacturonase 고생산 효모를 분리하였다. 분리된 strain CS-2 의 생리화학적인 실험을 수행한 결과 분리균주는 Diazonium bule B color test 와 urease test에서 양성반응, acetic acid, citrate acid 생성 시험, gelatin 분해시험, fat 분해시험에서는 음성반응을 나타났고, 탄소원의 조사에서 galactose를 탄소원으로 이용할 수 없었고, 질소원은 모두 이용할 수 없었다. 또한 $30^{\circ}C$ 에서는 성장하는 반면, $35^{\circ}C$ 이상에서는 성장을 하지 못하였고 50% 이상의 glucose 농도, 0.01% cyclohexamide 그리고 1% acetic acid에서 성장을 하지 못하였다. 현미경을 이용한 형태학적 관찰 결과, 크기는 $1.3{\times}2.9$$\mu$m인 타원형으로 관찰되었다, ,multiple budding을 하며 ascospore는 존재하는 반면 pseudomycelium과 true mycelium 은 존재하지 않았다. 이와 같은 결과를 종합하여 Cryptococcus 속의 특징과 유사함을 알 수 있었다. 분리된 Cryptococcus sp. CS-2 의 polygalacturonase 는 유도적으로 합성되어 catabolic repression 에 의해 조절됨을 알 수 있었으며, 3일 배양시 가장 높은 활성도를 나타내었으며, 고유 활성도는 2.50~2.55 units/mg 으로 나타났다. 이러한 polygalacturonase를 SDS-PAGE, activity staining 그리고 단일 탄소원에 따른 단백질 양상의 변화를 비교하여 분자량 측정한 결과 약 46KDa으로 나타났다.

• 대한기계학회논문집A
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• 제32권1호
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• pp.21-28
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• 2008

본 연구에서는 인장과 압축실험 데이터를 사용하여 재료의 응력-변형률 곡선을 얻었다. 시편에 네킹 현상이 발생하기 전에는 인장실험의 결과를 토대로 각 재료의 영률값과 항복강도를 찾았다. 이후 비선형 거동은 압축실험의 데이터를 이용해 나타냈다. 이렇게 얻어진 재료의 실제응력-변형률 곡선을 구간 멱함수법을 사용해 변형률 구간별로 회귀하였다. 회귀하여 구한 곡선과 실제 재료의 응력-변형률 곡선을 비교해 최적 회귀방법과 상응하는 변형률 회귀구간을 찾았다. 하나와 두 변수에 의한 회귀를 혼용하면 가장 적절한 회귀 방법이 얻어진다. 우선 두 변수들로 회귀하여 항복강도를 찾는다. 뤼더변형률이 없는 재료의 항복강도를 예측에는 초기구간 데이터만을 이용해야 오차를 최소화 할 수 있다. 한편 뤼더변형률이 재료들은 곡선의 후반부 데이터를 사용해야 정확한 물성치를 찾아낼 수 있다. 이어 항복 강도가 구해진 상황에서 응력-변형률 곡선의 전체 데이터를 사용해, n을 단일변수로 하여 회귀한다. 여기서 얻은 항복강도와 n을 이용하면 실제 실험 응력-변형률 곡선을 가장 유사하게 따라가는 회귀곡선을 얻을 수 있다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.170-177
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• 2003

This paper proposes a robust method for the Ramberg-Osgood (R-O) fit to accurately estimate elastic-plastic J from engineering fracture mechanics analysis based on deformation plasticity. The proposal is based on engineering stress-strain data to determine the R-O parameters, instead of true stress-strain data. Moreover, for practical applications, the method is given not only for the case when full stress-strain data are available but also for the case when only yield and tensile strengths are available. Reliability of the proposed method for the R-O fit is validated against detailed 3-D Finite Element (FE) analyses for circumferential through-wall cracked pipes under global bending using five different materials, three stainless steels and two ferritic steels. Taking the FE J results based on incremental plasticity using actual stress-strain data as reference, the FE J results based on deformation plasticity using various R-O fits are compared with reference J values. Comparisons show that the proposed R-O fit provides more accurate J values for all cases, compared to existing methods for the R-O fit. Advantages of the proposed R-O fit in practical applications are discussed, together with its accuracy.

• 열처리공학회지
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• 제4권3호
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• pp.1-6
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• 1991

In this study, intermediate thermo-mechanical treated Al-2.0 wt%Li, and Al-2.0 wt%Li-1.2 wt%Cu-1.0 wt%Mg-0.12 wt%Zr alloys were tested in tension at $10^{\circ}C$ and elevated temperature(100, 200 and $300^{\circ}C$). The results are follows : The tensile strength of Al-Li-Cu-Mg-Zr alloy is the highest but the elongation of Al-Li alloy is the highest(106%) among the all alloys in tension at $300^{\circ}C$. The Portervin-LeChartlier effect is showed in AI-Li-Cu-Mg-Zr alloy at 10 and $100^{\circ}C$, because of tangled dislocation by Mg and Cu. In the true stress-strain curves of all alloy, the peaks of stress at $300^{\circ}C$ are showed at the strain less than 0.1. In the binary alloy, the dynamic restoration process at 200 and $300^{\circ}C$ is nearly similar to dynamic recovery type. The hot deformation stress is decreased with increase of dynamic recovery degree, but the elongation is increased. When the strain the strain rate are constant, the temperature dependence of hot deformation stress is increased with increase of deformation temperature. The elongation and degree of dynamic recovery are decreased with increase of hot deformation activation energy, but the deformation stresses slightly increased.

• 대한기계학회논문집A
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• 제27권9호
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• pp.1571-1578
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• 2003

This paper proposes a robust method for the Ramberg-Osgood(R-O)fit to accurately estimate elastic-plastic J from engineering fracture mechanics analysis based on deformation plasticity. The proposal is based on engineering stress-strain data to determine the R-O parameters, instead of true stress-strain data. Moreover, for practical applications, the method is given not only for the case when full stress-strain data are available but also for the case when only yield and tensile strengths are available. Reliability of the proposed method for the R-O fit is validated against detailed 3-D Finite Element (FE) analyses for circumferential through-wall cracked pipes under global bending using five different materials, three stainless steels and two ferritic steels. Taking the FE J results based on incremental plasticity using actual stress-strain data as reference, the FE J results based on deformation plasticity using various R-O fits are compared with reference J values. Comparisons show that the proposed R-O fit provides more accurate J values for all cases, compared to existing methods for the R-O fit. Advantages of the proposed R-O fit in practical applications are discussed, together with its accuracy.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 춘계학술대회 논문집
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• pp.439-443
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• 2008

We investigate the mechanical behavior of a pre-heat treated steel of ESW95, which is being used for automotive parts including tie-rods to save manufacturing cost and enhance product quality. SCM435 is also investigated to reveal the characteristics of the pre-heat treated steel tested. AFDEX/MAT is used to extract the true stress-strain curve over the large strain with higher accuracy. It has been found that ESW95 has very week strain-hardening behavior which can be negligible compared with SCM435 and that the initial yield strength is quite high and the toughness of ESW95 reaches nearly 75% of SCM435. ESW95 is characterized by the weak strain-hardening behavior and high yield strength that can be lead to minimization of post-processing including heat treatment and straightening. ESW95 and SCM435 are also compared by applying them to ball-stud forging by computer simulation. It is expected that a great deal of change may take place in production as well as in service if the pre-heated steels are adopted.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.388-393
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• 2004

Creep behavior of Ti had been studied in a stress from 9.8 to 29.4 MPa and temperature rang from 873K to 973K with a special reference to tertiary creep. It was found that stress exponent of Ti was larger than that of the general pure metal and the compound metal. The relationship between true strain and strain rate in tertiary creep was appeared as the equation, $ln{\dot{e}}$ = $ln{\dot{e}}_{0}$ + ${\Omega}$ e Also, Apparent activation energy of was appeared as 274.92kJ/mol by using the equation ${\dot{\varepsilon}}_{0}$ = A ${\sigma }_{0}^{\ast_0}$ exp$(-Q_{0}/RT)$

• 대한기계학회논문집
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• 제19권2호
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• pp.597-604
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• 1995

When a pipe bend is modelled with straight beam elements, its stiffness, particularly in bending behavior, is overestimated than its true value. In this paper, we propose a simple and practical beam-modelling technique to estimate its stiffness properly. When this technique, based on the strain energy concept, is employed to modify the beam sectional properties of the bend, quite satisfactory results can be obtained. To verify the validity of this method, we apply the present technique to the free vibration analysis of a center pipe with 2 bends, one of the three components of the automobile exhaust system.

• Journal of Advanced Marine Engineering and Technology
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• 제33권4호
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• pp.539-544
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• 2009

The constraint effect $A_2$ has to be evaluated within plastic region near crack tip front using opening displacement. Plastic boundary and stress or strain conditions in the vicinity of the crack tip using recrystallization heat treatment was represented. It was found that the plastic deformation boundary by recrystallization heat treatment method was the true strain of ${\epsilon}t$ = 0.05mm/mm. With the estimation of constraint effects $A_2$, the region of proper displacement measurement point near crack tip was between 0mm and 1mm distance toward direction of crack propagation, and was between 1mm to 3mm distance toward direction of load line.

• Structural Engineering and Mechanics
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• 제63권2호
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• pp.251-257
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• 2017

A local damage identification method using measured structural static displacement is proposed in this study. Based on the residual force vector deduced from the static equilibrium equation, residual strain energy (RSE) is introduced, which can localize the damage in the element level. In the case of all the nodal displacements are used, the RSE can localize the true location of damage, while incomplete displacement measurements are used, some suspicious damaged elements can be found. A model updating method based on static displacement response sensitivity analysis is further utilized for accurate identification of damage location and extent. The proposed method is verified by two numerical examples. The results indicate that the proposed method is efficient for damage identification. The advantage of the proposed method is that only limited static displacement measurements are needed in the identification, thus it is easy for engineering application.