• Journal of Microbiology
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• 제44권5호
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• pp.492-501
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• 2006

In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of $40^{\circ}C$. The KNU5377 strain evidenced a very similar growth rate at $40^{\circ}C$ as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at $43^{\circ}C$. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and $H^+$-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures ($43^{\circ}C$), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.

• 한국가축번식학회지
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• 제10권2호
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• pp.168-174
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• 1986

These experiments were carried out to clarify the optimalconditions and interspecific cross reaction of H-Y antibody activity. H-Y antiserum was prepared in inbred SD female rats and Balb/c female mice by repeated immunization of rat newborn testis homogemate, rat and mouse spleen cells obtained from males of same strain. The activity of H-Y antibody in antiserum was tested by ELISA and biological tests. The cross reactivity of H-Y antibody was confirmed by culturing mouse and rabbit embryos in medium containing H-Y antibody and complement obtained from rat and guinea pig, respectively. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos in medium with different pH and complement concentration. The results obtained in these experiments were summarized as follows: 1. The formation rates of H-Y antibody in rats immunized with newborn testis and spleen cell were 40.0 and 50.0% respectively, and that in mouse immunized with spleen cell was 48.4%. 2. The activity of H-Y antibody was not affected by pH in range of 6.5 to 8.0, and the same was true for the relative concentration of complement to the H-Y antibody. 3. Minimum time needed for the activity of H-Y antibody was confirmed to be 0.5 to 1 hour and 24 to 48 hours respectively for the zona free embryos and intact embryos. 4. When mouse and rabbit embryos were treated with H-Y antibody obtained from rat, 46.4 and 54.8% of embryos were retarded or destroyed. From these results it could be said that H-Y antibody had strong interspecific cross reactivity.

• 대한기계학회논문집B
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• 제37권12호
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• pp.1153-1157
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• 2013

증기터빈의 터빈 블레이드는 발전소 핵심설비 중 하나로, 로터의 디스크에 결합되어 회전함으로 써 증기 에너지를 기계적 에너지로 변환시켜주는 역할을 하고 있다. 최근 터빈의 잦은 기동정지로 인해 블레이드 회전에 따른 원심하중이 반복적 작용하고 이에 따른 저압 증기터빈 최종단 블레이드의 손상이 자주 보고되고 있다. 본 논문에서는 터빈 블레이드에 발생되는 손상을 분석하여 블레이드에 발생되는 저주기 피로수명을 평가하였다. 증기터빈 최종단 블레이드의 균열발생 수명을 결정하기 위해 유한요소법으로 계산한 탄성응력에 Neuber's rule을 적용하여 진변형율 진폭을 계산하였으며, 예측된 수명과 블레이드 실제 기동정지횟수가 잘 일치됨을 보였다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.866-872
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• 2005

H. pylori is known to cause severe gastric diseases, including peptic ulcers and gastric cancers, and a link has also been suggested with iron-deficiency anemia (IDA). However, little is known about the pathogenesis of H. pylori-associated IDA. In the present study, to determine whether H. pylori strains are correlated with the prevalence of IDA, we analyzed and compared the sequences of the napA genes encoding a bacterioferritin-like protein in H. pylori strains. A total of 20 H. pylori strains were isolated from antral biopsies of patients with and without IDA, and the napA genes amplified from the genomic DNA were sequenced. A comparison of the deduced amino acid sequences for NapA revealed two sites with major variations. At residue 70, five out of the 12 non-IDA strains ($41.7\%$) contained serine, while only one of the 8 IDA strains ($12.5\%$) contained serine, indicating a significantly higher frequency of serine in the non-IDA strains. In addition, the NapA proteins from all 17 Western strains available on Web sites were found to contain serine residues at this position. Meanwhile, the other major variation was located at residue 73, where all eight IDA strains ($100\%$) contained leucine, while this was only true for eight of the 12 non-IDA strains ($66.7\%$). Therefore, these results indicated that the strains within each group were more genetically related to each other than to strains in the other group. When the expression level of the napA genes in the H. pylori strains was measured using RT-PCR, no significant difference was observed between the two groups, suggesting a similar intensity for the inflammatory responses induced by the NapA protein among the strains. Consequently, when taken together, the present data suggest that the occurrence of H. pylori-associated IDA may be partly determined by the infecting H. pylori strain, and the non-IDA strains are more closely related to Western strains than the IDA strains.

• 한국어병학회지
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• 제7권2호
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• pp.95-103
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• 1994

미꾸라지의 성장호르몬 유전자를 분리하기 위하여 미꾸라지의 cDNA library를 준비하였다. total RNA는 미꾸라지의 뇌하수체로부터 얻었으며 oligo (dT)-coupled magnetic bead를 이용하여 total RNA로부터 mRNA를 순수분리하였다. 정제된 mRNA는 cDNA를 합성하기 위한 기질로 사용하였으며, 합성된 cDNA는 EcoRV/Smal으로 절단된 pBlueKS+ plasmid vector에 삽입하였다. 모든 ligation 반응용액을 E. coli, JM109 균주에 형질전환을 유도하였으며 형질전환 효율을 최대화시키기 위하여 전기천공법을 이용하였다. 얻어진 모든 형질전환주들을 DIG로 표지된 Tilapia의 성장호르몬 유전자를 이용하여 고밀도 colony hybridization 에 의하여 검색하였다. 양성반응을 나타내는 10개의 형질전환주를 분리하여 2차 colony hybridization 및 southern hybridization에 의하여 성장호르몬 유전자가 cloning 되었음을 확인하였다. 10 개의 형질전환주 중 하나인 pCGH1을 probe로 사용한 Tilapia 성장 호르몬 유전자의 염기서열과 비교분석하였으며 53.2%의 유사성을 나타냄을 확인하였다.

• 한국지반신소재학회논문집
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• 제15권4호
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• pp.25-32
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• 2016

지반공학에서 평면변형율을 가정하는 2차원 사면안정해석은 일반적으로 널리 사용되고 있다. 이 가정은 사면활동이 넓은 영역에 걸쳐서 발생되는 가정이므로 3차원 효과가 무시된다. 대다수 연구에서 2차원해석의 최소안전율값은 3차원해석에 비하여 작게 평가되는 보수적인 결과를 나타낸다. 최근에는 컴퓨터의 소프트웨어와 하드웨어를 포함한 해석방법의 발달로 3차원해석에 대한 요구가 커지고 있다. 본 논문에서는 원호모드, 병진모드사면을 이용하여 유한요소에 의한 2, 3차원해석 및 2차원 한계평형해석을 통하여 수치해석을 실시하였다. 해석결과 매개변수(요소망크기, 체적팽창각(dilatency angle), 경계조건, 응력이력, 모델차원)에 따른 사면안정해석에 미치는 영향을 분석하였다. 해석결과 2차원 해석보다 3차원 해석에 의한 사면의 안전율 값은 항상 크게 평가되며, W 뱡향 경계조건이 롤러지지인 경우, 사면폭에 의한 결과 차이는 없는 것으로 조사되었다.

• Computers and Concrete
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• 제25권1호
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• pp.67-73
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• 2020

Traditionally used analytical approach to predict the fatigue failure of reinforced concrete (RC) structure is generally conservative and has certain limitations. The nonlinear finite element method (FEM) offers less expensive solution for fatigue analysis with sufficient accuracy. However, the conventional implicit dynamic analysis is very expensive for high level computation. Whereas, an explicit dynamic analysis approach offers a computationally operative modelling to predict true responses of a structural element under periodic loading and might be perfectly matched to accomplish long life fatigue computations. Hence, this study simulates the fatigue behaviour of RC beams with finite element (FE) assemblage presenting a simplified explicit dynamic numerical solution to show computer aided fatigue behaviour of RC beam. A commercial FEM package, ABAQUS has been chosen for this complex modelling. The concrete has been modelled as a 8-node solid element providing competent compression hardening and tension stiffening. The steel reinforcements are simulated as two-node truss elements comprising elasto-plastic stress-strain behaviour. All the possible nonlinearities are duly incorporated. Time domain analysis has been adopted through an automatic Newmark-β time incremental technique. The program consists of twelve RC beams to visualize the real behaviour during fatigue process and to obtain the reliability of the study. Both the numerical and experimental results indicate a redistribution of stresses along the time and damage accumulation of beam which severely affect the serviceability and ultimate capacity of RC beam. The output of the FEM analysis demonstrates good match with the experimental consequences which affirm the efficacy of the computer aided model. The controlled fatigue damage evolution at service fatigue load limits makes the FE model an efficient tool in predicting high cycle fatigue behaviour of RC structures.

• Smart Structures and Systems
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• 제6권3호
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• pp.183-196
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• 2010

Monitoring and economical design of alternative energy generators such as wind turbines is becoming increasingly critical; however acquisition of the dynamic output data can be a time-consuming and costly process. In recent years, low-cost wireless sensors have emerged as an enabling technology for structural monitoring applications. In this study, wireless sensor networks are installed in three operational turbines in order to demonstrate their efficacy in this unique operational environment. The objectives of the first installation are to verify that vibrational (acceleration) data can be collected and transmitted within a turbine tower and that it is comparable to data collected using a traditional tethered system. In the second instrumentation, the wireless network includes strain gauges at the base of the structure. Also, data is collected regarding the performance of the wireless communication channels within the tower. In both turbines, collected wireless sensor data is used for off-line, output-only modal analysis of the ambiently (wind) excited turbine towers. The final installation is on a turbine with embedded braking capabilities within the nacelle to generate an "impulse-like" load at the top of the tower. This ability to apply such a load improves the modal analysis results obtained in cases where ambient excitation fails to be sufficiently broad-band or white. The improved loading allows for computation of true mode shapes, a necessary precursor to many conditional monitoring techniques.

• Mycobiology
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• 제46권4호
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• pp.407-415
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• 2018

Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types ($A5B4{\times}A1B4$). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated.

• 대순사상논총
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• 제24_1권
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• pp.97-144
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• 2014

Jeungsan(甑山) carried out the Universal Redesign(天地公事) through which the former world full of a mutual opposition(相克) can be peacefully transferred into the latter world composed of a mutual beneficence(相生). Although the Universal Redesign consists of such three factors heaven, earth, and human, it converges towards Human Redesign in that humans are the main agents for heaven and earth. Human as Redesign object is characterized as a member of community, not an anarchistic individual. The Universal Resign of Jeungsan is eventually connected with the construction of a country comprising a mutual beneficence in that it is the most typical strain among all the human communities. His Universal Resign for foundering the foremost country does not target all the countries, but only one country upon and through which the world with a mutual beneficence can be formed. He executed the Universal Redesign based on such three principles as Haewon sangsaeng(解冤相生), Boeunsangsaeng(報恩相生), and Wonsibanbon(原始返本), Judging from such principles, Korea can be naturally selected as the target of the Universal Redesign for constructing the foremost country. It is approaching when the Universal Redesign in order to transform Korea into the foremost country comes true. The first step for Korea to be made as the foremost country is the construction of DMZ World Peace Park (DWPP) which was proposed by Bak Geunhye, the Korean President, to Obama, the President of USA, in 2013 she visited there. DWPP can be expected to be a center for corporation and interchange between South and North Korea under the interest and support of the world including UN, furthermore, the Capital area of reunified Korea, and finally the world hub of inter national political and economic relation. DWPP is planned to be built at the core of the best place in Korea which is a propitious spot all over the world, upon which a new world capitalism order armed with mutual beneficence can spread.