• Journal of Microbiology
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• v.44 no.5
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• pp.492-501
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• 2006

In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of $40^{\circ}C$. The KNU5377 strain evidenced a very similar growth rate at $40^{\circ}C$ as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at $43^{\circ}C$. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and $H^+$-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures ($43^{\circ}C$), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.

• Korean Journal of Animal Reproduction
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• v.10 no.2
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• pp.168-174
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• 1986

These experiments were carried out to clarify the optimalconditions and interspecific cross reaction of H-Y antibody activity. H-Y antiserum was prepared in inbred SD female rats and Balb/c female mice by repeated immunization of rat newborn testis homogemate, rat and mouse spleen cells obtained from males of same strain. The activity of H-Y antibody in antiserum was tested by ELISA and biological tests. The cross reactivity of H-Y antibody was confirmed by culturing mouse and rabbit embryos in medium containing H-Y antibody and complement obtained from rat and guinea pig, respectively. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos in medium with different pH and complement concentration. The results obtained in these experiments were summarized as follows: 1. The formation rates of H-Y antibody in rats immunized with newborn testis and spleen cell were 40.0 and 50.0% respectively, and that in mouse immunized with spleen cell was 48.4%. 2. The activity of H-Y antibody was not affected by pH in range of 6.5 to 8.0, and the same was true for the relative concentration of complement to the H-Y antibody. 3. Minimum time needed for the activity of H-Y antibody was confirmed to be 0.5 to 1 hour and 24 to 48 hours respectively for the zona free embryos and intact embryos. 4. When mouse and rabbit embryos were treated with H-Y antibody obtained from rat, 46.4 and 54.8% of embryos were retarded or destroyed. From these results it could be said that H-Y antibody had strong interspecific cross reactivity.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.12
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• pp.1153-1157
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• 2013

A steam turbine blade is one of the core parts in a power plant. It transforms steam energy into mechanical energy. It is installed on the rim of a rotor disk. Many failure cases have been reported at the final stage blades of a low-pressure (LP) turbine that is cyclically loaded by centrifugal force because of the repeated startups of the turbine. Therefore, to ensure the safety of an LP steam turbine blade, it is necessary to investigate the fatigue strength and life. In this study, the low cycle fatigue life of an LP steam turbine blade is evaluated based on actual damage analysis. To determine the crack initiation life of the final stage of a steam turbine, Neuber's rule is applied to elastic stresses by the finite element method to calculate the true strain amplitude. It is observed that the expected life and actual number of starts/stops of the blade were well matched.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.866-872
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• 2005

H. pylori is known to cause severe gastric diseases, including peptic ulcers and gastric cancers, and a link has also been suggested with iron-deficiency anemia (IDA). However, little is known about the pathogenesis of H. pylori-associated IDA. In the present study, to determine whether H. pylori strains are correlated with the prevalence of IDA, we analyzed and compared the sequences of the napA genes encoding a bacterioferritin-like protein in H. pylori strains. A total of 20 H. pylori strains were isolated from antral biopsies of patients with and without IDA, and the napA genes amplified from the genomic DNA were sequenced. A comparison of the deduced amino acid sequences for NapA revealed two sites with major variations. At residue 70, five out of the 12 non-IDA strains ($41.7\%$) contained serine, while only one of the 8 IDA strains ($12.5\%$) contained serine, indicating a significantly higher frequency of serine in the non-IDA strains. In addition, the NapA proteins from all 17 Western strains available on Web sites were found to contain serine residues at this position. Meanwhile, the other major variation was located at residue 73, where all eight IDA strains ($100\%$) contained leucine, while this was only true for eight of the 12 non-IDA strains ($66.7\%$). Therefore, these results indicated that the strains within each group were more genetically related to each other than to strains in the other group. When the expression level of the napA genes in the H. pylori strains was measured using RT-PCR, no significant difference was observed between the two groups, suggesting a similar intensity for the inflammatory responses induced by the NapA protein among the strains. Consequently, when taken together, the present data suggest that the occurrence of H. pylori-associated IDA may be partly determined by the infecting H. pylori strain, and the non-IDA strains are more closely related to Western strains than the IDA strains.

• Journal of fish pathology
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• v.7 no.2
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• pp.95-103
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• 1994

We have prepared cDNA libray of loach. M. mizolepis in order to isolate cDNA clone of growth hormone gene. Total RNA was isolated from pituitary of loach, and then mRNA was further purified from total RNA by oligo (dT)-coupled magnetic beads. The purified mRNA was used as substrates to prepare cDNA. The resulting cDNA was ligated into the EcoRV/Smal site of pBlueKS+. The ligation mixture have transformed E. coli JM109 strain with electroporator to obtain high yield of transformation efficiency. All the transformants was screened with DIG-labeled Tilapia growth hormone gene by high density colony hybridization. After isolating 10 putative colonies showing the positive signals, secondary colony hybridization and southern hybridization could confirm it as true clones. The nucleotide sequence of one candidate, pCGHI, was compared with 312 bp DNA fragment used as DNA probe and show 52% relative homology to Tilapia growth hormone gene.

• Journal of the Korean Geosynthetics Society
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• v.15 no.4
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• pp.25-32
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• 2016

The two-dimensional (2D) analysis is widely used in geotechnical engineering for slope stability analysis assuming a plane-strain condition. It is implicitly assumed that the slip surface is infinitely wide, and thus three-dimensional (3D) end effects are negligible because of the infinite width of the slide mass. The majority of work on this subject suggests that the 2D factor of safety is conservative (i.e. lower than the 'true' 3D factor of safety). Recently, the 3D finite element method (FEM) became more attractive due to the progress of computational tools including the computer hardware and software. This paper presents the numerical analyses on rotational mode and translational mode slopes using the 2D and 3D FEM as well as 2D limit equilibrium methods (LEM). The results of the parametric study on the slope stability due to mesh size, dilatency angle, boundary conditions, stress history and model dimensions change are analysed. The analysis showed that the factor of safety in 3D analysis is always higher than that in the 2D analysis and the discrepancy of the slope width in W direction on the factor of safety is ignored if the roller type of W direction conditions is applied.

• Computers and Concrete
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• v.25 no.1
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• pp.67-73
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• 2020

Traditionally used analytical approach to predict the fatigue failure of reinforced concrete (RC) structure is generally conservative and has certain limitations. The nonlinear finite element method (FEM) offers less expensive solution for fatigue analysis with sufficient accuracy. However, the conventional implicit dynamic analysis is very expensive for high level computation. Whereas, an explicit dynamic analysis approach offers a computationally operative modelling to predict true responses of a structural element under periodic loading and might be perfectly matched to accomplish long life fatigue computations. Hence, this study simulates the fatigue behaviour of RC beams with finite element (FE) assemblage presenting a simplified explicit dynamic numerical solution to show computer aided fatigue behaviour of RC beam. A commercial FEM package, ABAQUS has been chosen for this complex modelling. The concrete has been modelled as a 8-node solid element providing competent compression hardening and tension stiffening. The steel reinforcements are simulated as two-node truss elements comprising elasto-plastic stress-strain behaviour. All the possible nonlinearities are duly incorporated. Time domain analysis has been adopted through an automatic Newmark-β time incremental technique. The program consists of twelve RC beams to visualize the real behaviour during fatigue process and to obtain the reliability of the study. Both the numerical and experimental results indicate a redistribution of stresses along the time and damage accumulation of beam which severely affect the serviceability and ultimate capacity of RC beam. The output of the FEM analysis demonstrates good match with the experimental consequences which affirm the efficacy of the computer aided model. The controlled fatigue damage evolution at service fatigue load limits makes the FE model an efficient tool in predicting high cycle fatigue behaviour of RC structures.

• Smart Structures and Systems
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• v.6 no.3
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• pp.183-196
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• 2010

Monitoring and economical design of alternative energy generators such as wind turbines is becoming increasingly critical; however acquisition of the dynamic output data can be a time-consuming and costly process. In recent years, low-cost wireless sensors have emerged as an enabling technology for structural monitoring applications. In this study, wireless sensor networks are installed in three operational turbines in order to demonstrate their efficacy in this unique operational environment. The objectives of the first installation are to verify that vibrational (acceleration) data can be collected and transmitted within a turbine tower and that it is comparable to data collected using a traditional tethered system. In the second instrumentation, the wireless network includes strain gauges at the base of the structure. Also, data is collected regarding the performance of the wireless communication channels within the tower. In both turbines, collected wireless sensor data is used for off-line, output-only modal analysis of the ambiently (wind) excited turbine towers. The final installation is on a turbine with embedded braking capabilities within the nacelle to generate an "impulse-like" load at the top of the tower. This ability to apply such a load improves the modal analysis results obtained in cases where ambient excitation fails to be sufficiently broad-band or white. The improved loading allows for computation of true mode shapes, a necessary precursor to many conditional monitoring techniques.

• Mycobiology
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• v.46 no.4
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• pp.407-415
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• 2018

Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types ($A5B4{\times}A1B4$). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated.

• Journal of the Daesoon Academy of Sciences
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• v.24_1
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• pp.97-144
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• 2014

Jeungsan(甑山) carried out the Universal Redesign(天地公事) through which the former world full of a mutual opposition(相克) can be peacefully transferred into the latter world composed of a mutual beneficence(相生). Although the Universal Redesign consists of such three factors heaven, earth, and human, it converges towards Human Redesign in that humans are the main agents for heaven and earth. Human as Redesign object is characterized as a member of community, not an anarchistic individual. The Universal Resign of Jeungsan is eventually connected with the construction of a country comprising a mutual beneficence in that it is the most typical strain among all the human communities. His Universal Resign for foundering the foremost country does not target all the countries, but only one country upon and through which the world with a mutual beneficence can be formed. He executed the Universal Redesign based on such three principles as Haewon sangsaeng(解冤相生), Boeunsangsaeng(報恩相生), and Wonsibanbon(原始返本), Judging from such principles, Korea can be naturally selected as the target of the Universal Redesign for constructing the foremost country. It is approaching when the Universal Redesign in order to transform Korea into the foremost country comes true. The first step for Korea to be made as the foremost country is the construction of DMZ World Peace Park (DWPP) which was proposed by Bak Geunhye, the Korean President, to Obama, the President of USA, in 2013 she visited there. DWPP can be expected to be a center for corporation and interchange between South and North Korea under the interest and support of the world including UN, furthermore, the Capital area of reunified Korea, and finally the world hub of inter national political and economic relation. DWPP is planned to be built at the core of the best place in Korea which is a propitious spot all over the world, upon which a new world capitalism order armed with mutual beneficence can spread.