• Biomedical Science Letters
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• v.11 no.2
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• pp.165-173
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• 2005

A potent demethylating agent, 5-Azacytidine (5-AzaC) has been widely used as in many studies on DNA methylation, regulation of gene expression, and cancer biology. The mechanisms of the demethylating activity were known to be formation of complex between DNA and DNA methyltransferase (MTase), which depletes cellular MTase activity. However, 5-AzaC can also induce hypermethylation of a transgene in a transgenic cell line, G12 cells and it was explained as a result of defense mechanisms to inactivate foreign gene(s) somehow. This finding evoked the question that whether the phenomenon of hypermethylation induced by 5-AzaC is limited to the transgene or it can be occurred in endogenous gene(s). In order to answer the question, mutagenicity test of 5-AzaC and molecular characterization of mutants obtained from the test were performed using an endogenous gene, thymidine kinase (tk) in Chinese hamster V79 cells. When V79 and V79-J3 subclone cells were treated with 1, 2.5 ,5, $10{\mu}M$ of 5-AzaC for 48 hours, their maximum mutant frequencies were revealed as $6\times10^{-3}\;at\;5{\mu}M$(350-fold induction over background) and $8\times10^{-3}\;at\;2.5{\mu}M$ (l,800-fold induction over background) respectively. Since the induction rates were too high to be induced by true mutations, many trifluorothymidine (TFT)-resistant $(TFT^R)$ cells were subjected to Northern blot analysis to check the presence of tk transcripts. Surprisingly, all clones tested possessed the transcripts in a similar level, that implicates the $TFT^R$ phenotype induced by 5-AzaC has not given rise to hypermethylation of the gene in spite of unusually high mutation frequency. In addition, it has shown that the TK activity in the pool of 5-AzaC-induced $TFT^R$ cells has about a half of that in spontaneously-induced $TFT^R$ cells or in non-selected parental V79-J3 cells. This result suggests that the mechanism(s) underlying the TFT-resistance between spontaneously occurred and 5-AzaC-induced cells may be different. These findings have shown that the $TFT^R$ phenotype induced by 5-AzaC has not given rise to hypermethylation of the tk gene, and 5-AzaC may be induced by one or combined pathways among many drug resistance mechanisms. The exact mechanisms for the 5-AzaC-induced $TFT^R$ phenotype remain to elucidate.

• International Journal of Precision Engineering and Manufacturing
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• v.2 no.1
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• pp.18-25
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• 2001

Electrolytic polishing is the anodic dissolution process in the transpassive state. It removes non-metallic inclusion and improves mechanical and corrosion resistance of stainless steel. If there is a Bailby layer, it will be removed and the true structure of the surface will be restored. Electrolytic polishing is normally used to remove a very thin layer of material from the surface of metal object. A new electrolyte composed of phosphoric, sulfuric and distilled water has been developed in this study. Two current density, high & low current density regions, have been applied in this study. In this study, In the region of high current density, there is no plateau region but excellent electrolytic polishing effect can be accomplished in short machining time because material removel process and leveling process occur simultaneously. In the low current density region, there can be found plateau region. The material removal process and leveling process occur successively. The aim of this work is to determine electrolytic polishing for stainless steel in terms of high & low current density and workpiece surface roughness.

• Journal of the Society of Naval Architects of Korea
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• v.42 no.1 s.139
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• pp.34-42
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• 2005

An overview of 30 feet sailing yacht design is presented, with an emphasis on the factors contributing to start-up popularization. After prescribing the configurations of the purposed yacht, the design of the hull form with a rudder and a keel, are schematically described. Also the determinations of the dimensions of the sail and rig are performed. Finally this paper is associated with the state-of-the-art of calculating sailing yacht performance as this is performed in velocity prediction program (VPP). The VPP results shows a typical shape of a sailing yacht and the designed yacht has the best performance at 120 degree angle of true wind with 20 knots.

• Journal of Ship and Ocean Technology
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• v.10 no.2
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• pp.1-10
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• 2006

Numerical analysis of viscous flow around twin-skeg hull forms was conducted according to the variations of distance between skegs and vertical skeg inclinations by using a hydrodynamic analysis system, WAVIS. Six twin-skeg hull forms were derived by combining three distances between skegs (16m, 20m, 24m) and four vertical skeg angles ($0^{\circ},\;10^{\circ},\;15^{\circ},\;20^{\circ}$). It is found that the better resistance performance can be obtained with larger vertical skeg angle and smaller skeg distance for the present test cases. It also can be seen that the same trend is true for the nominal wake distributions in the propeller plane. Those tendencies were confirmed by the experimental results of MOERI. It is shown that numerical analysis can be a useful and practical tool for the evaluation and improvement of hydrodynamic performances for the complex stern hull forms with twin skegs.

• Journal of the Korean Applied Science and Technology
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• v.30 no.1
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• pp.96-115
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• 2013

This article describes the concept and the trend of antimicrobial coating agents, which will help to establish the direction of the research and development on antimicrobial coating agent. Antimicrobial agents are compounds that inhibit or kill microorganisms. They are classified into inorganic, metallic, low molecular weight organic, natural organic, and polymeric compounds. Antimicrobial coatings are applied to the surface of daily necessities, medical devices, industrial products, electrical appliances, fabrics, and interior building materials, etc. Conventional antibiotics penetrate microbes without damaging bacterial cell walls, leading to drug resistance which polymeric antimicrobials can prevent by disrupting cell walls. Most polymeric antimicrobials are focused on cationic polymers. Improvement in the selectivity and durability of antimicrobials and reduction of their toxicity will come true by more reasonable design of molecular structures and their combination in coating system.

• Journal of Advanced Navigation Technology
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• v.12 no.6
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• pp.653-658
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• 2008

The automotive environment is particularly demanding on connector performance, and is characterized by large temperature changes, high humidity and corrosive atmospheres. Fretting is a contact damage process that occurs between two contact surfaces. Fretting corrosion refers to corrosion damage at the asperities of contact surfaces. This damage is induced under load and in the presence of repeated relative surface motion, as induced for example by vibration. This paper critically reviews the works published previously on fretting corrosion of electrical connectors. Various experimental approaches such as testing machines, material selection, testing environments, acceleration testing techniques and preventing methods are addressed. Future research prospects arc suggested.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.388-389
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• 2015

After being enforced web board game enforcement ordinance, the majority manufacturer of sales that had been operating a web board game was greatly reduced. This restriction is true and that it had a significant impact in preventing exchange and gambling resistance, abnormal use has occurred to still bypass. Therefore, in this study, you can either leave a note of the 'Web Board' sunset enforcement ordinance, and how the debate any changes are necessary and alternative dimension approach, especially we try to study an unusual user patterns that utilize the game system of responsibility as an alternative.

• Steel and Composite Structures
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• v.39 no.3
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• pp.337-352
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• 2021

The study presented experimental and numerical investigation on the seismic performance of steel reinforced concrete (SRC) L-shaped column- reinforced concrete (RC) beam joints. Various parameters described as steel configuration form, axial compressive ratio, loading angle, and the existence of slab were examined through 4 planar joints and 7 spatial joints. The characteristics of the load-displacement response included the bearing capacity, ductility, story drift ratio, energy-dissipating capacity, and stiffness degradation were analyzed. The results showed that shear failure and flexural failure in the beam tip were observed for planar joints and spatial joint, respectively. And RC joint with slab failed with the plastic hinge in the slab and bottom of the beam. The results indicated that hysteretic curves of spatial joints with solid-web steel were plumper than those with hollow-web specimens. The capacity of planar joints was higher than that of space joints, while the opposite was true for energy-dissipation capacity and ductility. The high compression ratio contributed to the increase in capacity and initial stiffness of the joint. The elastic and elastic-plastic story deformation capacity of L-shaped column frame joints satisfied the code requirement. A design formula of joint shear resistance based on the superposition theory and equilibrium plasticity truss model was proposed for engineering application.

• International Journal of Highway Engineering
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• v.12 no.3
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• pp.113-119
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• 2010

This study presents experimental findings on the performance of cement concrete pavement incorporating mineral admixtures such as ground granulated blastfurnace slag and silica fume. Flexural strength, compressive strength, charge passed, diffusion coefficient of chloride ions and initial surface absorption of cement concrete pavement incorporating mineral admixtures were periodically measured and the corresponding results were compared to those of plain concrete pavement. As a result, strength behaviors of concrete pavement were dependent on the types of mineral admixtures. However, it was true that incorporation of silica fume had a beneficial effect on compressive strength development. Furthermore, the application of mineral admixtures led to a lower diffusion coefficient of chloride ions compared to plain concrete pavement. Based on the experimental results, the present study would be helpful to design high-performance cement concrete pavement.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.1
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• pp.125-130
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• 2007

Single-phase is used widely power of field of appliance because can use commercialized power directly without power transform. Specially, because condenser nu-drive style single-phase induction motor efficiency is excellent, and most suitable in embodiment as economical. It is established true that method by crossing self-discipline and method by revolving magnetic field are interpretation of net single-phase induction motor, but method to calculate electric motor inside proper move in existent theory or method is some complex. That arbor in the law of circuit constant that combine equivalent circuit law with numerical analysis law according to development of the latest computer or microprocessor is suggested and does the calculation processing fast and correct. In this paper, measurement wishes to present method that calculate after calculate digital measurement that measures correctly and measures impossible the first and the second leakage reactance and no-load test locked-rotor test, DC test and resistance measurement of stator winding in circuit parameter calculation program that is had for MatLab program individually in single-phase power.