• 제목/요약/키워드: Trolox equivalent antioxidant capacity (TEAC)

검색결과 38건 처리시간 0.024초

찰흑미를 첨가한 식빵의 항산화성에 관한 연구 (A Study on Antioxidant Activity of Bread with Waxy Black Rice Flour Added)

  • 김원모;이윤신
    • 한국조리학회지
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    • 제13권4호
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    • pp.178-185
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    • 2007
  • The antioxidant activities of the bread made from waxy black rice flour(WBRF) and wheat flour were investigated. The waxy black rice bread was manufactured with 10, 20, 30, or 40% waxy black rice flour(w/w). As the additive amount of WBRF were increased, DPPH(1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity and TEAC(trolox equivalent antioxidant capacity) were also increased. WBRF group showed the higher activity than the control group in DPPH radical scavenging activity and TEAC. The group with 10% WBRF added was not significantly different from the control group in metal chelating activity, but over 20% of addition, it had the higher metal chelating activity than that of the control group.

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Comparison of lymphocyte DNA damage levels and total antioxidant capacity in Korean and American diet

  • Lee, Min Young;Kim, Hyun A;Kang, Myung Hee
    • Nutrition Research and Practice
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    • 제11권1호
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    • pp.33-42
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    • 2017
  • BACKGROUND/OBJECTIVE: This study aims to measure the in vitro antioxidant capacity of Korean diet (KD) with American diet (AD) as a control group and to examine the ex vivo DNA damage reduction effect on human lymphocytes. MATERIALS/METHODS: The KD applied in this study is the standard one-week meals for Koreans (2,000 kcal/day) suggested by 2010 Dietary Reference Intakes for Koreans. The AD, which is the control group, is a one-week menu (2,000 kcal/day) that consists of foods that Americans would commonly take in according to the National Health and Nutrition Examination Survey. The antioxidant capacity of each menu was measured by means of the total phenolic assay and 3 in vitro antioxidant activity assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity ($ORAC_{ROO{\cdot}}$)), while the extent of ex vivo lymphocyte DNA damage was measured by means of the comet assay. RESULTS: When measured by means of TEAC assay, the in vitro antioxidant capacity of the KD of the day was higher than that of the AD (P < 0.05) while there was no significant difference in total phenolic contents and DPPH and ORAC assays. The ex vivo lymphocyte DNA damage protective effect of the KD was significantly higher than that of the AD (P < 0.01). As for the one-week menu combining the menus for 7 days, the total phenolic assay (P < 0.05) and in vitro antioxidant capacity (P < 0.001, DPPH; P < 0.01, TEAC) of the KD menu were significantly higher than those of the AD menu. Likewise, the ex vivo DNA damage reduction rate of the Korean seven-day menu was significantly higher than that of the American menu (P < 0.01). CONCLUSION: This study demonstrates that the high antioxidant capacity and DNA damage protective effect of KD, which consists generally of various plant foods, are higher than those of typical AD.

귀리 도정 부위별 메탄올 추출물의 항산화 성분 및 항산화 활성 (Antioxidant Compounds and Antioxidant Activities of Methanolic Extracts from Milling Fractions of Oat)

  • 함현미;우관식;박지영;이병원;최혜선;최용환;이준수;이유영
    • 한국식품영양과학회지
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    • 제45권11호
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    • pp.1681-1684
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    • 2016
  • 본 연구에서는 국내에서 개발된 귀리의 도정 부위별 메탄올 추출물의 항산화 성분 및 항산화 활성을 비교 분석하고자 하였다. 항산화 성분으로는 총 폴리페놀 및 플라보노이드 함량을 측정하였으며 항산화 활성은 ABTS 및 DPPH 라디칼 제거능, 환원력을 측정하였다. 실험 결과 귀리의 도정 부위별 메탄올 추출물 중 bran 추출물의 총 폴리페놀 및 플라보노이드 함량이 각각 41.53 mg gallic acid equivalents/100 g sample 및 12.07 mg catechin equivalents/100 g sample로 whole grain 및 endosperm 추출물보다 높은 함량이 함유되어 있는 것을 확인하였다. 또한, 이러한 항산화 성분이 다량 분포하는 bran 추출물의 ABTS 및 DPPH 라디칼 제거능, 환원력이 각각 42.34 mg Trolox equivalent antioxidant capacity(TEAC)/100 g sample, 24.18 mg TEAC/100 g sample, $A_{700}=0.76$으로 나타나 whole grain 및 endosperm 추출물보다 우수한 항산화 활성을 가지는 것으로 나타났다. 본 연구 결과는 귀리의 도정 부위별 항산화 성분 및 항산화 활성 연구에 있어 기초 자료로써 활용될 수 있을 것으로 생각하며, 우수한 항산화 성분 및 활성을 가지는 귀리의 bran층은 기능성 식품의 제조에 있어 좋은 소재로 활용될 수 있을 것으로 생각한다.

해면으로부터 분리된 Bromotopsentin의 항산화활성 (Antioxidant Activities of Bromotopsentin from the Marine Sponge Spongosorites sp.)

  • 이만기;김동규
    • 생약학회지
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    • 제44권3호
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    • pp.224-229
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    • 2013
  • Bromotopsentin (BSM) is a bisindole alkaloid compound, which is recognized as a metabolite of the marine sponge Spongosorites sp. In this study, the antioxidant activity of BSM was investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, the trolox equivalent antioxidant capacity (TEAC) assay, the superoxide radical scavenging (NBT) assay, the lipid peroxidation and hydroxyl radical-induced DNA damage assays were carried out to evaluate the antioxidant activity of BSM. It was found that BSM had stronger scavenging activity on the stable free radical DPPH and superoxide radical than L-ascorbic acid with an $IC_{50}$ value of 62 and 64 ${\mu}M$, respectively. The TEAC value which indicated the total antioxidant capacity of BSM was about 0.8, which was also stronger than L-ascorbic acid. About 1.3 ${\mu}M$ of BSM induced 50% inhibition of lipid peroxidation. 60 nM of BSM exhibited a significant protective activity against DNA strand scission by hydroxyl radical on pBR322 DNA. Taken together, we suggest that BSM possesses strong antioxidant activity, and could be a valuable new addition to the list of anti-aging chemotherapeutic agents.

Total antioxidant capacity of the Korean diet

  • Han, Jeong-Hwa;Lee, Hye-Jin;Cho, Mi Ran;Chang, Namsoo;Kim, Yuri;Oh, Se-Young;Kang, Myung-Hee
    • Nutrition Research and Practice
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    • 제8권2호
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    • pp.183-191
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    • 2014
  • BACKGROUND/OBJECTIVES: The objective of this study was to measure and/or estimate the total antioxidant capacity of the Korean diet. MATERIALS/METHODS: Eighty-one plant foods that were expected to exhibit rather high antioxidant activities were selected from the Korean diet using the Fifth Korean National Health and Nutrition Survey (KNHANES V). These foods were categorized into 11 food groups: cereals, potatoes, legumes, nuts, vegetables, kimchies, mushrooms, fruits, fruit juices, sea weeds, and oils. The foods were mixed in the proportions specified in traditional Korean recipes and analyzed. The measured indicators for antioxidant capacities were total phenolics, 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and Trolox equivalent antioxidant capacity (TEAC). RESULTS: Total phenolics were high in the fruit juices, nuts, vegetables, and fruits; and the average DPPH, ORAC, and TEAC values were high in the vegetables, fruits, fruit juices, and nuts. The correlation coefficient between the content of total phenolics of each food and the in vitro antioxidant capacity was relatively high at 0.851. The intake of total phenolics per capita per day in the Republic of Korea was estimated to be 127 mg. The total dietary antioxidant capacity (TDAC) values, which were obtained from the total antioxidant capacity of each food, taking into account the intake of each food, were 20,763, 54,335, and $876.4{\mu}mol$ of Trolox equivalents using the DPPH, ORAC, and TEAC methods, respectively. The food group that contributed the most to the Korean TDAC was cereals at 39.7%, followed by fruits and vegetables at 27.8% and 13.9%, respectively. The contribution of legumes, nuts, fruit juices, and mushrooms was quite minimal at less than 2% each. CONCLUSIONS: The content of total phenolics and the antioxidant capacity of the Korean diet are significantly correlated and the high contributing food groups are cereals, fruits, and vegetables.

가감온비탕합오산(加減溫脾湯合五散) 완제(完製)(HWW$^{(R)}$)의 항산화 효과에 대한 연구 (Antioxidative Activities of Wen-pi-tang-Hab-Wu-ling-san (WHW$^{(R)}$) in vitro)

  • 정진기;박용기
    • 대한한의학회지
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    • 제30권5호
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    • pp.146-156
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    • 2009
  • Objectives: The objective of this study was to investigate the antioxidant effects of manufactured Wen-pi-tang-Hab-Wu-ling-san (WHW$^{(R)}$) in vitro. Methods: WHW$^{(R)}$ was prepared by the pilot manufacture of WHW water extract from a GMP system appointed company. Antioxidative activities were determined by in vitro tests as follows: the scavenging activities of oxygen free radicals including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion, hydrogen peroxide and nitric oxide radicals, as well as ferrous ion chelating capacity and Trolox equivalent antioxidant capacity (TEAC). Results: WHW$^{(R)}$ significantly scavenged oxygen free radicals such as DPPH (IC$_{50}$=115.28 $\pm$ 0.25 $\mu$g/$m\ell$), superoxide anion (IC$_{50}$=8.56 $\pm$ 0.08 $\mu$g/$m\ell$), hydrogen peroxide (IC$_{50}$=240.36 $\pm$ 3.41 $\mu$g/$m\ell$) and nitric oxide (IC$_{50}$=162.28 $\pm$ 0.21 $\mu$g/$m\ell$) radicals. WHW$^{(R)}$ also showed ferrous ion chelating activity (IC$_{50}$=543.19 $\pm$ 4.85 $\mu$g/$m\ell$) and Trolox equivalent effects (IC$_{50}$=45.311 $\mu$g/$m\ell$) in TEAC and ORAC assay, respectively. Conclusion: This study demonstrates that WHW$^{(R)}$ has strong antioxidative properties through free radical scavenging activity. These data suggest that WHW$^{(R)}$ be used as an antioxidant agent.

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들깨유의 산화에 대한 들깨 발아 싹 추출물의 억제효과 (Inhibitory Effect of Perilla Sprouts Extracts on Oxidation of Perilla Oil)

  • 김석중
    • 한국응용과학기술학회지
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    • 제29권2호
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    • pp.330-338
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    • 2012
  • 들깨 종자를 10일 간 발아시키면서 얻어지는 싹에 대하여 일반성분 및 항산화력의 변화를 분석하였고, 들깨유 산화에 대한 싹 추출물의 억제효과를 조사하였다. 종자의 수분 함량(2.9%)은 발아 10일째 싹에서 9.2%로 증가하였으나 조회분 함량은 유의적인 변화가 없었다. 종자에서 46.8%와 20.7%이었던 조지방과 조단백질 함량은 발아 10일째 싹에서 각각 18.2%와 18.3%로 감소하였지만, 환원당과 조섬유 함량은 2.2% 및 14.8%에서 각각 12.8%와 22.4%로 증가하였다. 깻잎과 비교하면 10일째 싹은 조지방, 탄수화물, 환원당, 조섬유 함량이 더 높았고 수분, 조회분 및 조단백질 함량은 더 낮았다. 발아동안 항산화력은 증가하여 8일째에 최대치에 도달하였는데, DPPH와 ABTS 라디칼 소거능에 기반한 Trolox 당량 항산화력(TEAC)은 각각 133.1과 136.8 Trolox eq. mmol/kg이었으며, 3가 철이온 환원력(FRAP)는 399.3 Fe(II) eq. mmol/kg이었다. 폴리페놀 함량(19.2 g/kg)도 이 단계에서 가장 높았다. 한편, 깻잎은 발아 8일째의 싹과 유사한 TEAC를 보였으나 FRAP은 더 높았다. 발아 8일째 싹의 메탄올 추출물을 들깨유에 첨가 시 농도 의존적으로 산화가 억제되었는데, 대조 들깨유에서 1.67 hr이었던 산화유도기간이 2.5%(w/w) 추출물 첨가 들깨유에서는 4.62 hr로 나타나 들깨유의 산화 안정성이 약 2.8배 증가하였다. 이러한 산화유도기간은는 동일농도의 BHT 첨가구와 비교 시 약 38% 수준에 해당하였다.

천연물인 플라보노이드와 이소플라보노이드의 항산화 효과 비교연구 (Comparative Study of the Antioxidative Potential of Common Natural Flavonoids and Isoflavonoids)

  • 판데이 라메스 프리사드;코이라라 니런전;이주호;이희찬;송재경
    • 한국미생물·생명공학회지
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    • 제41권3호
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    • pp.367-371
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    • 2013
  • 2,2'-diphenylpicrylhydrazyl ($DPPH^{\cdot}$) assay와 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid ($ABTS^+$) decolorization assay는 자연상태의 플라보노이드와 이소플라보노이드의 항산화 활성을 확인하는데 사용된다. 억제중간값(half maximal inhibitory concentration ($IC_{50}$) values)과 트롤록스당량 항산화능값(trolox equivalent antioxidant capacity (TEAC) values)은 $DPPH^{\cdot}$ assay와 $ABTS^+$ assay로 계산되었다. DPPH assay 결과, 쿼세틴(quercetin)은 가장 강한 항산화 능력을 가졌고 뒤이어 피세틴(fisetin), 7,8-디하이드록시플라본(7,8-dihydroxyflavone), 모린(morin), 캠퍼롤(kaempferol) 순이었다. 쿼세틴, 피세틴, 7,8-디하이드록시플라본은 부틸하이드록시 아니솔(butyl hydroxyl anisole)보다 더 높은 항산화 능력을 가졌다. 쿼세틴은 플라보노이드와 이소플로보노이드 중에서 TEAC 값이 가장 높았고 뒤이어 3-하이드록시플로본(3-hydroxyflavone), 피세틴, 7,8-디하이드록시플라본과 모린 순이었다. 다른 나머지 플라보노이드와 이소플라보노이드는 트롤록스 보다 더 약한 $ABTS^+$ 분해능력(scavenging potential)을 가졌다. 테스트된 13개 플라보노이드/이소플라보노이드에서 이소플라보노이드는 플라보노이드보다 매우 약한 항산화 능력을 보였다.

아로니아 첨가 비율에 따른 프리저브 잼의 품질 특성 및 항산화 활성 (Quality characteristics and antioxidant activities of jams according to varying ratios of aronia)

  • 임상휘;김경철;김주성
    • 한국식품과학회지
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    • 제54권2호
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    • pp.141-146
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    • 2022
  • 본 연구는 생리활성물질이 풍부한 아로니아를 활용하여 가공식품 개발에 필요한 기초 연구 데이터를 제공하기 위해 아로니아와 설탕의 배합비율별 프리저브 잼을 제조한 뒤 그 품질 특성과 항산화 활성을 조사하였다. 배합비율에 따라 아로니아의 함량이 낮아질수록 당도는 높아졌으며 수분함량은 낮아지는 경향을 나타내었다. 강도와 최대응력은 아로니아 함량이 줄어들고 설탕의 함량이 증가할수록 높은 값을 나타내었다. 잼의 비율별 항산화 활성은 아로니아 함량 70%에서 DPPH radical 저해율이 가장 높았다. 총 페놀 함량, TEAC, FRAP, ORAC의 값은 모두 아로니아 함량에 따라 비례적으로 증가하는 경향을 나타내었다. 관능평가에서 단맛과 식감이 모두 강하다고 평가된 아로니아 함량 30%에서는 오히려 종합적 선호도에서 가장 낮게 나타났으며 아로니아 함량 40%에서 가장 높은 선호도를 나타내었다. 본 연구에서는 아로니아와 설탕 배합비율에 따른 품질 특성과 항산화 연구를 진행하였으며 국산 아로니아를 활용한 제품의 개발에 기초 자료로서 활용될 것으로 기대된다.

Quantitative Structure-Activity Relationship (QSAR) of Antioxidative Anthocyanidins and Their Glycosides

  • Chang, Hyun-Joo;Choi, Eun-Hye;Chun, Hyang-Sook
    • Food Science and Biotechnology
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    • 제17권3호
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    • pp.501-507
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    • 2008
  • The quantitative structure-activity relationships (QSAR) study of antioxidative anthocyanidins and their glycosides were evaluated using 4 different assays of Trolox equivalent antioxidant capacity (TEAC), superoxide radical ($O_2^{{\cdot}-}$), hydrogen peroxide ($H_2O_2$), and peroxynitrite radical ($ONOO^-$) scavenging with TSAR software. Four models were developed with significant predictive values ($r^2$ and p value), which indicated that the antioxidant activities were mainly governed by the 3-dimensional structural energy (torsional energy), constitutional properties (the number of hydroxyl and methyl groups), and electrostatic properties (heat of formation, and dipole, quadrupole, and octupole components). This QSAR approach could contribute to a better understanding of structural properties of anthocyanidins and their glycosides that are responsible for their antioxidant activities. It might also be useful in predicting the antioxidant activities of other anthocyanins.