• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2005년도 총회 및 춘계학술발표회
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• pp.178-181
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• 2005

토양세척 공정 시 오염물질을 제거하기 위해 사용한 계면활성제를 재사용하기 위해 활성탄을 사용하였으며 이때 계면활성제와 HOC (Hydrophobic Organic Chemical)의 분배를 예측 할 수 있는 모델을 개발하였다. 계면활성제로는 Triton X-100, HOC는 phenanthrene을 활성탄으로는 Darco 20-40 mesh를 사용하였다. 개발된 모델로 계면활성제 주입량, 활성탄 주입량에 따른 계면활성제와 HOC의 분배결과를 각각 전산 모사를 통하여 얻었다. 그 결과 활성탄이 포함된 계 내에서의 계면활성제와 HOC의 분배가 미셀이 형성되는 농도를 전후로 서로 다른 분배 양상을 보였다. 또한 전산모사 결과는 실제 실험결과를 비교적 정확하게 반영함을 알 수 있다.

• Bulletin of the Korean Chemical Society
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• 제23권7호
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• pp.943-947
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• 2002

The two reactions between iron ion (Fe3+ ) and o-chlorophenylfluorone (o-CPF) and copper ion (Cu2+ ) and o-CPF are sensitive at pH 6 in the presence of Triton X-100. We have determined the formation constants of the complexes by the spectral correction technique. Because of the poor selectivity of o-CPF to metals, the competition coordination of only the iron ion from the Cu-o-CPF complex was found and applied to the selective detection of iron traces by the Selective Competition Coordination Determination (SCCD) approach.The analysis of several samples shows that the relative standard deviations are less than 5.0% and the recovery of iron ions between 94.5% and 106%.

• BMB Reports
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• 제28권6호
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• pp.552-555
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• 1995

Treatment of microsomal vesicles isolated from etiolated Pisum sativum L cv. Alaska epicotyl tissue with agents inhibiting protein dephosphorylation, namely NaF and/or ATP, resulted in increased binding of the phytotropin NPA to the putative auxin efflux carriers localized on the plasma membrane. The phytotropin effect was especially conspicuous if the vesicles were simultaneously treated with Triton X-100. Kinetic analysis of the binding indicated the existance of two distinct sites for NPA, each having different affinities. Increased binding of the phytotropin to the membrane where protein dephosphorylation was inhibited was attributable to the increased ligand affinity of both sites. Treatment of tissue segments with flubride was found to enhance in vivo auxin transport. Implications of covalent modification of the auxin efflux carrier complex for the regulation of membrane transport of auxin molecules are discussed.

• Bulletin of the Korean Chemical Society
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• 제27권11호
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• pp.1780-1784
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• 2006

Cloud point extraction was used to extract mefenamic acid (MF) from human urine, and spectrofluorimetry and spectrophotometry were used to analyze extracted MF. The variables affecting extraction and phase separation, i.e. HCl and Triton X-114 concentration, temperature and time of equilibration, were optimized. Under the experimental conditions used the limit of detection for extraction of 25 mL of sample was 0.006 and 0.045 mg $L^{-1}$, with relative standard deviations of 2.52 and 1.45% (n = 5) for spectrofluorimetric or spectrophotometric methods, respectively. Good recoveries in the range of 95-107% were obtained for spiked samples. The proposed methods were applied to the determination of MF in human urine.

• 미생물학회지
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• 제26권1호
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• pp.52-59
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• 1988

A partially purified preparation of L-galactonolactone oxidase which catalyzes the last step of L-ascorbic acid biosynthesis was obtained from Saccharomyces cerevisiae ATCc 26787. The purification procedures included Triton X-100 treatment, protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose CL-6B ion exchange chromatography, Sephadex G-150 gel filtration chromatography, and Phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The optimum temperature for the enzyme activity was about $34^{\circ}C$ and the optimum pH was 6.8-7.0. The substrate specificity was confined to L-aldonolactones, L-galactono-1,4-lactone and L-gulono-1,4-lactone. An apparent Km value of 0.294mM with L-galactono-1,4-lactone as a substrate was found. By comparing the substrate specificities of this enzyme with those of isofunctional enzymes of higher plants and animals, it becomes evident that the enzyme of S. cerevisiae ATCC 26787 is rather similar to the L-gulonolactone oxidase of animals than the galactonolactone dehydrogenase of higher plants.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 C
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• pp.1906-1908
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• 2003

본 연구는 biosensor용 microchannel 제작에 사용되는 PDMS의 표면 개질화와 표면 환원 현상을 해결 하기위한 co-polymer를 이용한 표면 처리특성에 관한 연구이다. PDMS 와 PDMS co-polymer의 표면 개질화를 위해 $O_2$ RF Plasma를 사용하였으며 10W, 1.5sccm의 분위기 하에서 표면 처리 공정을 수행하였다. 실험에 사용된 co-polymer는 소수성과 친수성의 성질을 동시에 갖는 triton x-100과 PDMS를 혼합하여 제작하였다. 각각 흔합비(0.5:100, 1:100, 1.5:100, 2:100)가 다른 시료를 제작하여 플라즈마 표면처리공정을 통해 표면처리를 하였으며, 표면 처리된 PDMS의 표면 특성변화에 비교 검증하였다.

• 한국환경농학회지
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• 제34권3호
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• pp.238-243
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• 2015

BACKGROUND: Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS: We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

• Journal of Microbiology
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• 제43권5호
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• pp.437-442
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• 2005

Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on $13\%$ SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.

• Bulletin of the Korean Chemical Society
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• 제21권11호
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• pp.1090-1094
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• 2000

A reaction between germanium (Ge) and the ligand, o-chlorophenylfluorone (o-CPF) has been carried out. The reaction sensitive at pH 4.5 in the presence of triton x-100 was selective in the presence of EDTA. The spectral correction technique was ap plied to the analysis of the reaction instead of single wavelength spectrophotometry because the absorption of excess of o-CPF was not negligible. An updated determination of the properties of the Ge(IV)-o-CPF complex is given, which involved the complex ratio, stepwise absorptivity and stability constant of the complex. In present work, the results show that the complex $Ge(o-CPF)_3was$ formed and its cumulative stability constant was 1.09 ${\times}$1016 . For sample analysis, the detection limit of germanium was 0.01 mg/L, and the recoveries were between 96.4% and 102%, with relative standard deviations of less than 6.5%.

• Bulletin of the Korean Chemical Society
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• 제22권6호
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• pp.565-569
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• 2001

The spectral correction matrix has been established to study the simultaneous complextions between metals and a ligand, and it eliminated the mutual influence among the complexes. The two sensitive reations between iron(Ⅱ) and 1-(6-bromo-2-benzothiazolylazo)-2-naphthol(BBTAN) and zinc (Ⅱ) and BBTAN have been investigated and applied to the simultaneous determination of Zn and Fe at pH 8.5 in the presence of triton x-100. This method gave the simples determination of the characteristic constants of the complexes. For the analysis of natural water, foods and other samoles, the recoveries of Fe and Zn were between 92 and 109% and between 91 and 104%, and their relative standard deviations were less than 5.5 and 11%, respectively.