• 동의생리병리학회지
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• 제19권2호
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• pp.441-445
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• 2005

Tripterygium regelii has been used as an oriental medicine, especially antiparasitic, anti-inflammatory and detoxifying agents in East asia. During our research to develop new antitumor agents from natural products, MeOH ext. and CH2Cl2 ext. of Tripterygium regelii showed the potent antitumor activity. In order to purify active compounds from Tripterygium regelii, activity-guided fractionation was carried out. Silica gel and RP-18 column chromatography for the active fraction led to the isolation of two compounds and their antitumor activities were studied. Those two compounds didn't show potent antitumor activity against human tumor cell lines. The structure of two compounds were determined by $^1H-NMR$, $^{13}C-NMR$, DEPT, $^1H-^{13}C$ COSY and IR spectrum. Compound I and Compound II were turned out to be Celastrol, and ${\beta}-sitosteryl-3-o-{\beta}-D-glucopyranoside$ respectively.

• 동의생리병리학회지
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• 제20권5호
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• pp.1196-1199
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• 2006

Tripterygium regelii SPRAGUE is distributed in Korea and Northern China. This extract has been used as a herb medicine, especially antiparasitic, anti-inflammatory and detoxifying agent in East asia. During our research to develop new antitumor agents from natural products, Dichlorornethane (CH$_2$Cl$_2$) extract of Tripterygium regelii SPRAGUE (DTR) showed the potent apoptotic effects in A-549 lung cancer, HeLa-3 cervical cancer, SKMEL-2 melanoma cells in a dose-dependent manner. in order to purify major compounds from DTR, column chromatography was carried out gradually. Silica gel and RP-18 column chromatography for active fractions led to the isolation of a compound. The compound determined by 1 H-NMR was turned out to De Celastrol known to have antitumor activity.

• 식물분류학회지
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• 제51권3호
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• pp.319-325
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• 2021

Tripterygium wilfordii Hook.f. (Celastraceae), collected from Phia Oac-Phia Den National Park of Vietnam, is reported here as a new generic and species record for the flora of Vietnam. The genus Tripterygium differs from other genera of Celastraceae by its samara fruits, which become three-winged at maturity, and a scandent shrub. Taxonomic notes, descriptions, line drawing and photographs are provided, together with short notes on the distribution, ecology and phenology of Tripterygium wilfordii Hook.f. (Celastraceae).

• Archives of Pharmacal Research
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• 제12권4호
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• pp.310-312
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• 1989

Three new sesquiterpene alkaloids, tripterregeline, A, B and C were isolated from the stem bark of Tripterygium regelii and their partial structures were determined by various spectroscopic methods.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.77.1-77.1
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• 2008

• Journal of the Korean Wood Science and Technology
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• 제26권2호
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• pp.24-28
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• 1998

Ray cells with perforations are recorded for the first time in the Korean Celastraceae species of Euonymus sieboldiana and Tripterygium regelii and the Oleaceae species of Abeliophyllum distichum, Forsythia ovata, Ligustrum japonicum, and Osmanthus heterophylla, All these anomalous ray cells have simple perforations, and the vessel elements of all these species have simple perforation plates, Thus, in the Korean Celastraceae and Oleaceae, the perforations of ray cells appear to be identical with the types of perforation plates in the vessel elements of the same wood, The diagnostic value of the perforated ray cells is also discussed.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.823-834
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• 2014

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of $50{\mu}M$ methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas $50{\mu}M$ salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of $500{\mu}M$ had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.

• 한국산림과학회지
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• 제99권4호
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• pp.590-596
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• 2010

지리산국립공원 천왕봉지역을 중심으로 분포하고 있는 가문비나무의 생육현황과 가문비나무림의 구조를 정확하게 파악하고자, 가문비나무가 주로 생육하고 있는 지역에 33개의 방형구($20{\times}20$ m)를 설치하여 식생을 조사하였다. 고사한 가문비나무는 주로 상층수관의 나무에서 발견되었으며, 개체수의 비율로는 15.6%이었고, 유묘의 개체수도 매우 적게 나타났다. Cluster 분석한 결과 두개의 집단으로 분류되었다. 개회나무와 당단풍, 미역줄나무, 졸참나무; 좀고채목과 괴불나무; 나래회나무와 시닥나무; 당단풍과 미역줄나무 등의 수종들 간에는 높은 정의상관이 인정되었고, 가문비나무와 구상나무; 좀고채목과 시닥나무; 미역줄나무와 시닥나무 등의 수종들 간에는 약한 부의상관이 인정되었다. 조사지의 군락별 종다양성지수는 1.0000~1.3010범위로 나타났다.

• Journal of Plant Biology
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• 제2권2호
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• pp.1-21
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• 1959

1. The plant communities on Mt. Sulak, a typical alpine mountain in central Korea was researched from the standpoint of ecological survey. 2. The plant on this mountain can largely be classified into 2 categories, the warm zone (below 1100m) and the frigid zone. The former is again classified into a broad-leaved tree formation and a needle-leved tree formation, and the latter is calssified into a broad-leaved tree formation and needle-leaved tree formation, and shrub formation. 3. The broad-leaved tree formation in the warm zone can largely be classified into a Carpinus laxiflora-syringa Palibiniana var. kanibayashi association(1), a Carpinus erosa-Acer Pseudo-sieboldianun association(2), and a Quercus mongolica- Tripterygium Regelii association(3). 4. The needle-leaved tree formation in the warm zone can also largely ben classified into a Pinus densiflora- Miscanthus sinensis association(4), Abies holophylla- Tripterygium regelii association(5), Pinus kordiensis- Rhododendron schlippenbachii association(6) In the association(4), the canopy of the Pinus densiflora is gradually being occupied by the Quercus mongolica which is one kind of broad-leaved trees. 5. We can fully see a DryoPteris type of the flour layer plant type in the association (3) and also see a Sasamorpha type in the association (5). 6. The broad-leaved tree formation in the frigid zone can largely be classified into Quercus mongolica- Rhododendron schlippenbachii association (8). The constituents of tree and subtree layers in (7) and (8) associations are similar to those association (3), but the constituents of the shrub layer are different from those of associtation (3) due to the difference in height. 7. The needle-leaved formation in the frigid zone can largely be classified into Thuja koraiensisRodgersia podophylla var. viritis association (9) and Abies nephrolepis-Acer tschonoskii var. rubripes asscciation (10). The species of alpine plants or subalpine plants are gradually increased in this formation. 8. The shrub formation can also largely be classified into a Rhododendron mucronulatum- Patrinia saniculaefolia association (11) and Pinus pumila associatio (12). Association (12) has largely developed on the windy place. Association (12) was burned due to the bombing during the Korean war, but now we recoginized there occurred an invasion of Tripterygium Regelii in such a place. 9. The herb layer species which constitute the shrub formation are mostly alpine or subalpine plants of small size, and their kinds are also very few. 10. The growth of the Moss layer is especially good because of the varied conditions of the habitat. The kinds which can easily be seen are the genus of Thunidium, Haplocladium, Brachythecium, Macromitrium, Holonitrium, Atrichum, Schwstchkeopsis, Grimmia, Hedwigia, Rhynchostegium and Mnium. 11. The genus of the Sphagnum densly grows like a mat at the acid and moisturous place above 1100m. The authors should express their thanks to father. Yang Kisup, Dean of Catholic Medical College, Prof. Yoon Doksuen, from their valuable advice. Thanks arealso due to Prof. Numata, Chiba University, Prof. Takagi, Nagoya University and Prof. Ando, Hiroshima University in Japan for his kind help and identification of Bryophytes.

• 한국산림과학회지
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• 제112권4호
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• pp.405-416
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• 2023

본 연구는 미역줄나무 출현에 따른 분비나무의 지속성에 대해 분석하고자 하였다. 총 140개소를 대상으로 현장조사를 수행하였고, 미역줄나무의 출현유무와 출현하는 층위 기준에 따라 미역줄나무가 교목·아교목층에 출현하는 유형(TP), 관목층에 출현하는 유형(SP), 모든 층위에서 출현하지 않는 유형(A)으로 유형을 구분하였다. 지표종 분석에서 TP유형과 SP유형에서 미역줄나무가 지표종으로 나타났으며, MRPP-test를 통해 군집별 이질성을 확인하였다. 치수와 흉고직경급 분석 결과, TP유형에서 분비나무의 어린나무 발생량이 가장 낮게 나타나 지위경쟁에 어려움이 있는 것으로 판단된다. 본 연구를 통해 분비나무의 식생구조를 이해하고 보전 및 관리에 기여하고자 한다.