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Study on the Antitumor Activity of Dichloromethane Extract of Tripterygium regelii SPRAGUE  

Park, Wan-Su (Department of Korean medicine, The Armed Forces Byukje Hospital)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.5, 2006 , pp. 1196-1199 More about this Journal
Abstract
Tripterygium regelii SPRAGUE is distributed in Korea and Northern China. This extract has been used as a herb medicine, especially antiparasitic, anti-inflammatory and detoxifying agent in East asia. During our research to develop new antitumor agents from natural products, Dichlorornethane (CH$_2$Cl$_2$) extract of Tripterygium regelii SPRAGUE (DTR) showed the potent apoptotic effects in A-549 lung cancer, HeLa-3 cervical cancer, SKMEL-2 melanoma cells in a dose-dependent manner. in order to purify major compounds from DTR, column chromatography was carried out gradually. Silica gel and RP-18 column chromatography for active fractions led to the isolation of a compound. The compound determined by 1 H-NMR was turned out to De Celastrol known to have antitumor activity.
Keywords
Tripterygium regelii SPRAGUE; apoptotic effect; A-549; HeLa-3; SKMEL-2; Celastrol;
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