• Title/Summary/Keyword: Triplex

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Effect of Surface Treatment on Adhesive Bonding Strengh of Composite Material for Cryogenic Application (극저온용 복합재료의 접착부 강도에 미치는 표면처리 효과에 대한 연구)

  • Ahn, Myoung-Ho;So, Yong-Shin;Park, Dong-Hwan
    • Proceedings of the KWS Conference
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    • 2010.05a
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    • pp.28-28
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    • 2010
  • The secondary barrier of cargo containment for membrane LNG tank is composed of composite materials such as rigid triplex (rigid secondary barrier, RSB) and flexible triplex (flexible secondary barrier, FSB). RSB and FSB are adhered to each other using an epoxy adherent and the quality of the secondary barrier depends on the bonding strength between them. The bonding strength between RSB and FSB is greatly influenced by the surface condition of RSB prior to joining. In this study, the effect of surface condition prior to joining on the joint strength and the fracture mode occurred between RSB and FSB have been examined in order to establish a proper surface treatment method for improving the bonding strength at the temperature of $-170^{\circ}C$.

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Stabilization of Poly(dA)·[poly(dT)]₂Triple Helical DNA by Acridine Derivatives: Role of Side Chain in the Triplex Stabilization

  • 현경미;이길준;조태섭;Kim, Seog K.;이세윤
    • Bulletin of the Korean Chemical Society
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    • v.18 no.5
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    • pp.528-534
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    • 1997
  • The conformation and stabilization effects of acridine derivatives 9-aminoacridine, an acridine with an aminoalkyl side chain, bis acridine (two acridines linked by an aminoalkyl side chain), and proflavin in the triplex helical poly(dA)·[poly(dT)]2 were investigated by optical spectroscopies. Based on the negative LD and weak positive CD in the acridine absorption wavelength region, we concluded that the acridine moiety of all derivatives are intercalated. We also examined the melting temperatures. Of all the compounds examined, the acridine with an amino alkyl side chain had the strongest effect on the stabilization of the third strand of a poly (dA)·[poly(dT)]2 triplex. The role of the side chain, based on this observation, is discussed.

Design and Test of Oxidizer-Rich Triplex Injector Preburner (산화제 과잉 삼중분사기 예연소기 개발 시험)

  • Ha, Seong-Up;Moon, Il-Yoon;Kang, Sang-Hun;Moon, In-Sang;Lee, Soo-Yong
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2012.05a
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    • pp.76-80
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    • 2012
  • Uni-element preburners using a oxidizer-rich triplex injector have been designed and tested. During combustion tests 1L mode high-frequency instability of 1100 Hz and low-frequency instability of 100 Hz were observed. High-frequency instability has been suppressed by reducing chamber diameter and applying turbulent rings in combustion chamber. Recently, research to reduce low-frequency instability is in progress.

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A Thermodynamic Investigation into the Stabilization of Poly(dA).[poly(dT)]2 Triple Helical DNA by Various Divalent Metal Ions

  • Choi, Byung-Hoon;Yeo, Ga-Young;Jung, Jin-Ah;Lee, Bae-Wook;Han, Sung-Wook;Cho, Tae-Sub
    • Bulletin of the Korean Chemical Society
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    • v.30 no.11
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    • pp.2691-2696
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    • 2009
  • Effects of representative group II and transition metal ions on the stability of the $poly(dA){\cdot}[poly(dT)]_2$ triplex were investigated by the van’t Hoff plot constructed from a thermal melting curve. The transition, $poly(dA){\cdot}[poly(dT)]_2\;{\rightarrow}\;poly(dA){\cdot}poly(dT)\;+\;poly(dT)$, was non-spontaneous with a positive Gibb’s free energy, endothermic (${\Delta}H^{\circ}$ > 0), and had a favorable entropy change (${\Delta}S^{\circ}$ > 0), as seen from the negative slope and positive y-intercept in the van’t Hoff plot. Therefore, the transition is driven by entropy change. The $Mg^{2+}$ ion was the most effective at stabilization of the triplex, with the effect decreasing in the order of $Mg^{2+}\;>\;Ca^{2+}\;>\;Sr^{2+}\;>\;Ba^{2+}$. A similar stabilization effect was found for the duplex to single strand transition: $poly(dA){\cdot}poly(dT)\;+\;poly(dT)\;→\;poly(dA)\;+\;2poly(dT)$, with a larger positive free energy. The transition metal ions, namely $Ni_{2+},\;Cu_{2+},\;and\;Zn_{2+}$, did not exhibit any effect on triplex stabilization, while showing little effect on duplex stabilization. The different effects on triplex stabilization between group II metal ions and the transition metal ions may be attributed to their difference in binding to DNA; transition metals are known to coordinate with DNA components, including phosphate groups, while group II metal ions conceivably bind DNA via electrostatic interactions. The $Cd_{2+}$ ion was an exception, effectively stabilizing the triplex and melting temperature of the third strand dissociation was higher than that observed in the presence of $Mg_{2+}$, even though it is in the same group with $Zn_{2+}$. The detailed behavior of the $Cd_{2+}$ ion is currently under investigation.

Helical Periodicity of $(dT)_n{\cdot}(dA)_n{\cdot}(dT)_n$ Triple - Stranded DNA

  • Kim, Ki-Hyun;Koo, Hyeon-Sook
    • BMB Reports
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    • v.30 no.6
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    • pp.426-430
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    • 1997
  • The helical periodicity of the triple-stranded $(dT)_n{\cdot}(dA)_n{\cdot}(dT)_n$ sequence was determined by measuring gel-mobilities of bent DNA fragments containing the sequence. In the bent DNA fragments, a $GA_{22}G$ $CT_{22}C$ sequence was located between two bent DNA loci composed of six $A_{6}{\cdot}T_{6}$ repeats. and the DNA length between the bent DNA loci was varied by 1 base pair over a full helical turn. The gel mobility of each bent DNA fragment reflected the overall extent of DNA bending and varied with the DNA length between the two bent loci. Mobilities of the bent DNA fragments in 5% polyacrylamide gel were measured after preincubating the DNA fragments both in the presence and absence of $CT_{22}C$ oligonucleotide. By comparing the bent DNA fragments containing an intermolecular triplex structure with those of a genuine duplex structure in the gel mobilities, the helical periodicity of the $T_n{\cdot}A_n{\cdot}T_n$ triplex DNA was determined to be $11.5({\pm}0.3)bp/turn$.

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Characterization of Electrical Crosstalk in 1.25 Gbps Optoelectrical Triplex Transceiver Module for Ethernet Passive Optical Networks (이더넷 광 네트워크 구현을 위한 1.25 Gbps 광전 트라이플렉스 트랜시버 모듈의 전기적 혼신의 분석)

  • Kim Sung-Il;Lee Hai-Young
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.3 s.333
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    • pp.25-34
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    • 2005
  • In this paper, we analyzed and measured the electrical crosstalk characteristics of a triplex transceiver module for ethernet Passive optical networks(EPONS). And we improved the electrical crosstalk levels using Dummy ground lines with signal lines. The triplex transceiver module consists of a laser diode as a transmitter, a digital photodetector as a digital data receiver, and a analog photodetector as a community antenna television signal receiver. And there are integrated on silicon substrate. The digital receiver and analog receiver sensitivity have to meet -24 dBm at $BER=10^{-l2}$ and -7.7 dBm at 44 dB SNR. And the electrical crosstalk levels have to maintain less than -86 dB from DC to 3 GHz. From analysis and measurement results, the proposed silicon substrate structure that contains the Dummy ground line with $100\;{\mu}m$ space from signal lines and separates 4 mm among devices respectively, is satisfied the electrical crosstalk level compared to simple structure. This proposed structure can be easily implemented with design convenience and greatly reduced the silicon substrate size about $50\%$.

Effect of Ethidium on the Formation of Poly(dA)·[poly(dt)]₂Triplex: A Kinetic Study by Optical Spectroscopic Methods

  • 이길준;현경미;조태섭;Kim, Seog K.;정맹준;한상욱
    • Bulletin of the Korean Chemical Society
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    • v.19 no.4
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    • pp.444-449
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    • 1998
  • The kinetics of the formation of triplex $poly(dA){\cdot}[poly(dT)]_2$ from poly(dA)·poly(dT) and poly(dT) is examined by various optical spectroscopic methods, including absorption, circular and linear dichroism (LD) spectroscopy. In the pseudo first order condition, where the poly(dT) concentration is kept lower than that of duplex, the association of the poly(dT) is enhanced by the presence of ethidium; the rate constant is proportional to the amount of ethidium in the mixture. When the concentration of the duplex and the single strand is the same, a spectral change is explained by double exponential curves, indicating that at least two steps are involved, the fast association and slow rearrangement steps. In contrast to the pseudo first order kinetics, the association step in an equimolar condition is not affected by the presence of ethidium. In the rearrangement step, the magnitude of LD decreases with an increase in ethidium concentration, suggesting that the bending of polynucleotide around the intercalation site occurs in the rearrangement step.