• Bulletin of the Korean Chemical Society
• /
• v.30 no.5
• /
• pp.1039-1042
• /
• 2009

RNA polymerase II C-terminal domain phosphatase 1 containing ubiquitin like domain (UBLCP1) has been identified as a regulatory molecule of RNA polymerase II. UBLCP1 consists of ubiquitin like domain (UBL) and phosphatase domain homologous with UDP and CTD phosphatase. UBLCP1 was cloned into the E.coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using both affinity and gel-filtration chromatography. Domains of UBLCP1 protein were successfully purified as 7 mg/500 mL (UBLCP1, 36.78 KDa), 32 mg/500 mL (UBL, 9 KDa) and 8 mg/500 mL (phosphatase domain, 25 KDa) yielded in LB medium, respectively. Isotope-labeled samples including triple-labeled ($^2H/^{15}N/^{13}C$) UBLCP1 were also prepared for hetero-nuclear NMR experiments. $^{15}N-^{1}H$ 2D-HSQC spectra of UBLCP1 suggest that both UBL and phosphatase domain are properly folded and structurally independent each other. These data will promise us further structural investigation of UBLCP1 by NMR spectroscopy and/or X-ray crystallography.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.26 no.3
• /
• pp.9-15
• /
• 2012

In this paper, we deal with a surface discharge that caused an aggravation of the dielectric strength in the $N_2/O_2$ mixture gas, When composit dielectrics were formed from the use of a solid dielectric. It was found from this study that the surface discharge voltage was deeply involved in the mixture ratio of $O_2$, the electrical property of the solid dielectric, kind of the solid dielectric, an electric field at the triple junction and a medium effect. These results expect basic data that will be used to transmission and distribution power system equipment using the $N_2/O_2$ mixture gas.

• Journal of Communications and Networks
• /
• v.15 no.4
• /
• pp.422-429
• /
• 2013

Advances in wireless sensor network (WSN) technology have enabled small and low-cost sensors with the capability of sensing various types of physical and environmental conditions, data processing, and wireless communication. In the WSN, the sensor nodes have a limited transmission range and their processing and storage capabilities as well as their energy resources are limited. A triple umpiring system has already been proved for its better performance in WSNs. The clustering technique is effective in prolonging the lifetime of the WSN. In this study, we have modified the ad-hoc on demand distance vector routing by incorporating signal-to-noise ratio (SNR) based dynamic clustering. The proposed scheme, which is an efficient and secure routing protocol for wireless sensor networks through SNR-based dynamic clustering (ESRPSDC) mechanisms, can partition the nodes into clusters and select the cluster head (CH) among the nodes based on the energy, and non CH nodes join with a specific CH based on the SNR values. Error recovery has been implemented during the inter-cluster routing in order to avoid end-to-end error recovery. Security has been achieved by isolating the malicious nodes using sink-based routing pattern analysis. Extensive investigation studies using a global mobile simulator have shown that this hybrid ESRP significantly improves the energy efficiency and packet reception rate as compared with the SNR unaware routing algorithms such as the low energy aware adaptive clustering hierarchy and power efficient gathering in sensor information systems.

• Journal of Electrical Engineering and information Science
• /
• v.2 no.5
• /
• pp.17-26
• /
• 1997

Interconnect characterization on a wafer level was performed. Test patterns for single, two-coupled, and triple-coupled lines ere designed by using 0.5$\mu\textrm{m}$ CMOS process. Then interconnect capacitances and resistances were experimentally extracted by using tow port network measurements, Particularly to eliminate parasitic effects, the Y-parameter de-embedding was performed with specially designed de-embedding patterns. Also, for the purpose of comparisons, capacitance matrices were calculated by using the existing CAD model and field-solver-based commercial simulator, METAL and MEDICI. This work experimentally verifies that existing CAD models or parameter extraction may have large deviation from real values. The signal transient simulation with the experimental data and other methodologies such as field-solver-based simulation and existing model was performed. as expected, the significantly affect on the signal delay and crosstalk. The signal delay due to interconnects dominates the sub-micron-based a gate delay (e.g., inverter). Particularly, coupling capacitance deviation is so large (about more than 45% in the worst case) that signal integrity cannot e guaranteed with the existing methodologies. The characterization methodologies of this paper can be very usefully employed for the signal integrity verification or he electrical design rule establishments of IC interconnects in the industry.

• Journal of Contemporary Eastern Asia
• /
• v.20 no.2
• /
• pp.85-107
• /
• 2021

Wildfires have become increasingly common and intense in South Korea because of climate change, but few have recognized the catastrophic level of the problem. Given the significant impact of wildfires, emergency management stakeholders must have effective risk communication structures for rapidly responding to such phenomena and overcoming geographical difficulties. Despite the country spending billions of dollars to build a big databased early warning system, risk communication flow during the 2017 Gangneung wildfire was ineffective, thereby causing substantial economic, social, and environmental losses. To examine the patterns of information exchange in South Korea's risk communication networks and their structural characteristics during the wildfire, we conducted semantic and network analyses of real-time data collected from social media. The results showed that the inefficient flow of risk information prevented emergency responders from adequately assessing the emergency and protecting the population. This study provides new insights into effective risk communication responses to catastrophic events and methods of research on webometric approaches to emergency management.

• Journal of Contemporary Eastern Asia
• /
• v.20 no.2
• /
• pp.259-277
• /
• 2021

This article investigates the geopolitics of the energy transition era, concentrating on China's solar photovoltaic (PV) industry. Authors have noted that the rise of renewables is changing the geopolitical landscape of world energy systems, but these new energy sources carry their own technical characteristics and geopolitical implications. Bearing this in mind, this research answers the questions: What are the structural factors that facilitate China's use of renewable energy to achieve political goals, and what are their implications? In order to analyze the data, I devise an analytical framework based on the energy statecraft literature and contrast rival explanations, particularly the "prosumer theory" and the premise of less geopolitical interdependence in a renewable-centered world. I show that asymmetric interdependence in the solar PV sector is already a reality. China's solar PV industry is a case that suffices all conditions (centrality in industrial capacity, market share, and companies' compliance, but to a lesser extent in critical materials and technological endowments) in the solar PV sector to devise effective strategies aimed at reaping benefits out of its asymmetric interdependence with the rest of the world.

• Journal of Contemporary Eastern Asia
• /
• v.20 no.2
• /
• pp.108-128
• /
• 2021

Research on the news coverage of North Korea has been paying less attention to social media platforms than to legacy media. An increasing number of social media users post, retweet, share, interpret, and set agendas on North Korea. The accessibility of international users and North Korea's publicity purposes make social media a venue for expression, news diversity, and framing about the nation. This study examined the sentiment of Twitter posts on North Korea from a framing perspective and the relationship between network strengths and sentiment from a social network perspective. Data were collected using two tools: Jupyter Notebook with Python 3.6 for preliminary analysis and NodeXL for main analysis. A total of 11,957 tweets, 10,000 of which were collected using Python and 1,957 tweets using NodeXL, about North Korea between June 20-21, 2020 were collected. Results demonstrated that there was more negative sentiment than positive sentiment about North Korea in the sampled Twitter posts. Some users belonging to small network sizes reached out to others on Twitter to build networks and spread positive information about North Korea. Influential users tended to be impartial to sentiment about North Korea, while some Twitter users with a small network exhibited high percentages of positive words about North Korea. Overall, marginalized populations with network bonding were more likely to express positive sentiment about North Korea than were influencers at the center of networks.

• Biomedical Science Letters
• /
• v.27 no.3
• /
• pp.134-141
• /
• 2021

c-Jun N-terminal kinases (JNKs) have a Janus face, regulating both cell apoptosis and survival. The present study focused on understanding the function of JNK in tumor development and the chemoresistance underlying JNK-mediated cancer cell survival. We identified an inhibitor of JNK1, an important regulator of cancer cell survival. Kinase assay data showed that JNK1-dependent c-Jun phosphorylation was inhibited by indirubin derivatives. In particular, indirubin-3-monoxime (I3M) directly inhibited the phosphorylation of c-Jun in vitro, with a half inhibition dose (IC₅₀) of 10 nM. I3M had a significant inhibitory effect on JNK1 activity. Furthermore, we carried out assays to determine the viability, migration, and proliferation of breast cancer cells. Our results demonstrated that cell growth, scratched wound healing, and colony forming abilities were inhibited by the JNK inhibitor SP600125 and I3M. The combination of SP600125 and I3M significantly decreased cancer cell proliferation, compared with either SP600125 or I3M alone. Our studies may provide further support for JNK1-targeting cancer therapy using the indirubin derivative I3M in breast cancer.

• Journal of Contemporary Eastern Asia
• /
• v.21 no.2
• /
• pp.27-46
• /
• 2022

The purpose of this study is two-fold: first, to discuss the concept of health diplomacy and the Korean government's response to contain the COVID-19 pandemic; second, to assess and compare assumptions of variances about foreigners' perceptions of how Korea is leveraging digital technology in battling the coronavirus spread, and its vaccine campaign; through the lenses of Chinese, Filipino, and Pakistani foreign nationals who are currently living in Korea. A total of 219 foreigners responded to the survey. The collected data were analyzed as percentages, mean averages, t-test, and ANOVA for statistical analysis. Results show that Korea is utilizing its digital technology practices and vaccine campaign in battling the pandemic through efforts of health diplomacy. ANOVA indicated significant results and assumptions of variance across three ethnic groups showing the Pakistani population had higher mean scores than the Chinese and Filipino about Korea's health diplomacy during the pandemic. This study contributes to the literature on Korea's digital technology practices and vaccine campaigns amidst the COVID-19 pandemic by promoting its image through health diplomacy efforts. It projects the country's soft image on a global scale, to save the lives of locals and foreign nationals, by providing insights into health diplomacy in Korea.

• Journal of Contemporary Eastern Asia
• /
• v.19 no.2
• /
• pp.84-103
• /
• 2020

Spurred by the ongoing globalization, an increase in mobility has diversified migrant categories and strengthened intercultural rapport. Alongside the "traditional" migrants, "White" (Caucasian) individuals are coming into greater focus of migration studies as "lifestyle migrants". Although White migrations are not a new phenomenon, the deep-seated idea of White supremacy continues to play an important role in contemporary intercultural communication, awarding Whites across communities a "cosmopolitan" status of highly educated cultural elites. As such, the focus of this research is on highly skilled White European migrants, on their subjective experiences of integration in Japan, and whether they perceive Whiteness as an obstacle or an advantage in this process, if integration is desired at all. To discern the connection between race and integration, this research investigates the non-White majority society of Japan as it has established racial hierarchies according to the Western models, consequently influencing the status of its contemporary White immigrants. Privileged, yet singled out as racial and cultural role models, White Europeans' integration seemingly becomes nearly impossible. The data obtained in fifteen semi-structured interviews confirms that Whiteness grants advantages when entering the Japanese job market, but remains an obstacle in everyday community integration. European professionals do not feel accepted and abandon efforts to integrate, if such were made, retreating into "cosmopolitan islets" wherein they renegotiate their White European identities.