• 공업화학
• /
• 제4권3호
• /
• pp.505-514
• /
• 1993

올리고글리세롤 모노라우레이트 에스테르류를 단계적인 합성 방법으로 monoglycerol monolaurate, diglycerol monolaurate, symmetrical triglycerol monolaurate 및 symmetrical tetraglycerol monolaurate 등을 85~95%의 높은 수율로 각각 제조하였다. 그리고 이 화합물들에 대한 TLC 및 관크로마토그래피를 행하여 순수한 물질로 잘 분리 정제하였고, IR 및 $^1H\;NMR$로 구조를 확인하였다.

• Journal of Microbiology and Biotechnology
• /
• 제30권4호
• /
• pp.561-570
• /
• 2020

This study was designed to synthesize triglycerol monolaurate (TGML) with Lipozyme 435 as the catalyst, and explore its effects on the growth of Aspergillus parasiticus (A. parasiticus) and Aspergillus flavus (A. flavus) and the secretion of aflatoxin b1. The highest content of TGML (49.76%) was obtained at a molar ratio of triglycerol to lauric acid of 1.08, a reaction temperature of 84.93℃, a reaction time of 6 h and an enzyme dosage of 1.32%. After purification by molecular distillation combined with the washes with ethyl acetate and water, the purity of TGML reached 98.3%. Through characterization by electrospray-ionization mass spectrometry, infrared spectrum and nuclear magnetic resonance, the structure of TGML was identified as a linear triglycerol combined with lauroyl at the end. Finally, the inhibitory effects of TGML on the growths of A. parasiticus and A. flavus and the secretion of aflatoxin b1 were evaluated by measuring the colony diameter, the inhibition rate of mycelial growth and the content of mycotoxin in the media. The results indicated that TGML had a stronger inhibitory effects on colony growth and mycelial development of both toxic molds compared to sodium benzoate and potassium sorbate, and the secretions of toxins from A. parasiticus and A. flavus were completely suppressed when adding TGML at 10 and 5 mM, respectively. Based on the above results, TGML may be used as a substitute for traditional antifungal agents in the food industry.