• The Journal of Internal Korean Medicine
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• v.26 no.3
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• pp.551-562
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• 2005

Objectives : The aim of this study was to investigate the effect of Trichosanthis Semen Herbal-acupuncture(TS-HA) at Joksamni(ST36) on ovalbumin-induced asthma in mice. Methods : C57BL/6 mice were sensitized and challenged with ovalbumin(OVA) once a week for twelve weeks. The experimental group was treated with 1% concentrations of TS-HA at Joksamni(ST36) three times a week for the last eight weeks. Results : 1. The weight and total lung cells of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 2. Total leukocytes and eosinophils in Bronchoalveoler-lavage fluid(BALF) of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 3. Eosinophils in BALF of the mice in the group treated with TS-HA in photomicrographs decreased significantly compared with those of control group. 4. According to histological analysis of lung sections, adhension of collagen in TS-HA decreased significantly compared with that of control group. 5. The concentration of IgE, IL-4, IL-5 in BALF and IL-4, IL-5, IL-13 in serum of the mice in the group treated with TS-HA decreased significantly compared with that of control group. 6. The number of $Gr-^+/CD11b^+,\;CD11b^+,\;CD3e^-/CCR3^+,\;CD4^+,\;CD8^+\;CD3e^+/CD69^+,\;CD23^+/B220^+\;cells$ in the lungs of the mice in the group treated with TS-HA decreased significantly compared with those of control group. Conclusion : These results suggest that Trichosanthis Semen-herbal acupuncture at Joksamni(ST36) done on C57BL/6 mice is effective in part in relieving OVA-induced asthma in C57BL/6 mice.

• Korean Journal of Pharmacognosy
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• v.54 no.2
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• pp.80-87
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• 2023

Rancidity changes were examined for 6 herbal medicines, namely Persicae Semen, Armeniacae Semen, Lini Semen, Trichosanthis Semen, Arecae Semen, Myristicae Semen known to have relatively high fat content. In order to reduce rancidity of herbal medicines, samples were stored at 3 different conditions of room, refrigerating and freezing temperatures, and the rancidity was measured for 10 months with every 2 month interval. Fat content was extracted by using ethyl ether, and acid values and peroxide values, which are generally accepted indicators of fat rancidity, were measured. When storing Persicae Semen, Lini Semen and Arecae Semen at room temperature, the acid values increased as the storage period increased, and it was higher than when stored in refrigeration or freezing. The measurement of peroxide value showed more significantly higher initial degree of rancidity when Persicae Semen, Trichosanthis Semen, Arecae Semen and Myristicae Semen were stored at room temperature. It was observed that storing herbal medicines in refrigeration or freezing inhibited their rancidity compared to storing them at room temperature. To investigate the quality changes according to rancidity, the analysis of aflatoxins and indicator components showed that aflatoxins B₁ and B₂ were detected in Armeniacae Semen, Arecae Semen and Myristicae Semen, and the amount of amygdalin was well maintained within the specification standard.

• The Journal of Dong Guk Oriental Medicine
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• v.4
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• pp.237-265
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• 1995

The inhibitory effects of Gleditschiae Spina, Cleditschia saponin, two complex prescriptions such as Takrisodokum and Kwaluubangtang, which contain Gleditschiae Spina, and their altered prescriptions on the activity of cyclooxygenase in vitro were investigated. The water extract of Gleditschiae Spina and Gleditschia saponin markedly inhibited the cyclo-oxygenase activity by 59% and 67%, respectively, at a concentration of $1mg/m{\ell}$, on the other hand, Takrisodokum and Kwaluubangtang showed 15% and 7% inhibition, respectively. The extracts with the substraction of Gleditschiae Spina and/or Platycodi Radix inhibited to a much smaller extent than Takrisodokum. However, in case of the extracts with the substraction of Gleditschiae Spina and/or Trichosanthis Semen, only one prescription, which does not contain Trichosanthis Semen, exhibited the same inhibition as Takrisodokum. From above results, Gleditschiae Spina was found to be most important for the effect of Takrisodokum and Kwaluubangtang on their antiinflammatory action. Instead of Gleditschiae Spina, another parts of this plant were added to both complex prescriptions and tested according to above methods, indicating that Gleditschiae Spina is more important than another parts for both prescriptions. When the administered dose of Gleditschiae Spina is increased(twofold and threefold) in both complex prescriptions, the inhibitory effect in Takrisodokum was diminished. however, the effect in Kwaluubangtang was increased.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.336.2-336.2
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• 2007

See Full Text

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.3
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• pp.175-190
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• 2006

Purpose : This study was conducted to investigate the in vitro inhibitory effects of heat-clearing medicinal on common bacterias in gynecology. Methods : The heat-clearing medicinals ( Trichosanthis Radix, Sophorae Fructus, Phragmitis Rhizoma, Buddleiae Flos, Bambusae Folium, Anemarrhenae Rhizoma, Celosiae Semen, Gardeniae Fructus, Prunellae Spica, Sophorae Radix, Dictamni Radicis Cortex, Coptidis Rhizoma, Gentianae Scabrae Radix, Scutellariae Radix, Phellodendri Cortex) were used in this study. Staphylococcus aureus, MRSA, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Streptococcus spp., Escherichia coli HB101, Lactobacillus gasseri were used for normal vaginal florae. We evaluated antibiotic effect by the optical density and the colony test. Results : The optical density and colony test showed that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex of herbs among heat-clearing medicinal had antimircobial effect. Celosiae Semen and Prunellae Spica had antimicrobial susceptibility and selective toxicity in MRSA. Scutellariae Radix and Phellodendri Cortex had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex among heat-clearing medicinal be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.710-715
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• 2005

This research was to investigate inhibitory activity of Sasang medicines on Obesity. 56 kind of herb medicines to powder, abstract add 100 times methanol. Examine inhibitory effect against lipase activity in vitro. It was compared Xenical(Orlistat) with 56 kind of herb medicines. Inhibitory effect against lipase activity of Xenical as a standard was 93.3%. It is Inhibitory effect against lipase activity of Taeyangin herb medicine. (Fructus Pruni Tomentosae 77.7% Cortex Acanthopanacis73.5%, Fructus Chaenomelis73.1%, rice bran on a mallet head68.1%, Semen Fagopyri 48.1%, Radix Vitis 31.5%) It is Inhibitory effect against lipase activity of Soyangin herb medicine. (Radix Saposhnikoviae 91.5%, Semen Plantaginis 90.4%, Semen Trichosanthis 89.2%, Herba Schizonepetae 85.7%, Radix Euphorbiae Kansui 76.1%, Rhizoma Anemarrhenae 76.0%, Cortex Phellodendri 75.1%, Herba Menthae 74.8%, Radix Angelicae Pubescentis 61.2%, Rhizoma Alismatis 62.6%, Poria 60.9%, Rhizoma Notopterygii 22.5%, Radix Peucedani 18.2%, Caulis Akebiae 17.7%) It is Inhibitory effect against lipase activity of Taeeumin herb medicine. (Herba Spirodelae 91.4%, Radix Polygalae 88.3%, Arillus Longanae 84.3%, Radix Platycodi 81.7%, Semen Zizyphi Spinosae 80.2%, Spina Gleditsiae 79.7%, Herba Ephedrae 75.3%, Semen Raphani 73.1%, Rhizoma Cimicifugae 73.0%, Rhizoma Acori Graminei 71.8%, Flos Chrysanthemi71.0%, Radix et Rhizoma Rhei 68.4%, Vermiculus Holotrichia 59.9%, Radix Puerariae 55.3%, Fructus Schizandrae 53.4% Semen Coicis 50.6%, Semen Biotae 47.5%, Semen Nelumbinis 46.7%, Radix Angelicae Dahuricae 45.1%, Semen Castaneae 44.4%, Rhizoma Ligustici 34.5%, Tuber Liriopis 16.4%, Radix Scutellariae4.2%) It is Inhibitory effect against lipase activity of Soeumin herb medicine. (Pericarpium Arecae89.9%, Rhizoma Alpiniae Officinarum 89.5%, Radix Polygoni Multiflori81.2%, Rhizoma Cyperi 79.0%, Cortex Magnoliae 72.8%, Radix Aucklandiae 72.0%, Rhizoma Zingiberis 71.9%, Pericarpium Citri Reticulatae Viride 71.3%, Radix Cynanchi Wilfordi 57.7% Rhizoma Pinelliae 36.7%, Fructus Alpiniae Oxyphyllae 35.8% Fructus Aurantii Immaturus 17.5%, Pericarpium Citri 16.1%) Therefore, Radix Saposhnikoviae(91.5) and Herba Spirodelae(91.4%) were the most effective medicice of 56 kind of medicines.

• Journal of Sasang Constitutional Medicine
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• v.14 no.2
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• pp.147-152
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• 2002

One woman, Sixty three years old, suffered from chest pain, abdominal pain, nausea and vomiting for about three months. Although many examinations, brain magnetic resonance imaging(MRI), chest computed tomography(CT), abdominal CT, gastric endoscope, abdominal ultrasonography, and so forth, was performed from every point of view, those were non-significant. At first, I wrongly diagnosed her case as disease of Soumin and gave Soumin Gwakhyangjeongkisan. But she complained of diarrhea and chest discomfort. I re-diagnosed her case as Soyangin Gyeolhyung and treated with Hyeongbangdojeoksan, herbal medicine, consisted of Rhizoma Rehmanniae(生地黃), Lignum Akebiae(木通), Radix Scrophulariae(玄蔘), Semen Trichosanthis(瓜蔞仁), etc. Three weeks have passed from first visit and she did not suffered from pain. I experienced the excellent effect of Hyeongbangdojeoksan to Soyangin Gyeonlhyung.

• The Journal of Internal Korean Medicine
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• v.27 no.2
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• pp.394-406
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• 2006

Backgrounds and Objectives: Asthma is considered to be an inflammatory disease characterized by airway hyperresponsiveness and Pulmonary eosinophilia. And it is known the structure and function of IL-5, its receptor and the mechnism IL-5 triggered eosinophil accumulation and inflammaion of the airways. At this point of view, we assume which oriental materia medics can the splenocyte inhibit from secreting the IL-S in vitro. Material and Methosds: We used the splenocyte of mouse 8 weeks after its birth, and then cnltivated those into the 2 experimental groups and control group for 48 hours. The culture medium of experimental groups were made of $1{\mu}g/ml,\;10{\mu}g/ml$, oriental materia medics, representative. And the culture media of control group was given no oriental materia medica. Then, we assayed the quantity of cytokine-expression by the Sandwich ELISA. The quantifies of cytokine-expression of the experimental groups were compared with that of the control group which was standardized These method were used for the all of oriental materia medica treated. Results: In this study, we demonstrated that 12 oriental materia medica that inhibit the splenocyte from secreting the IL-5 in both $1{\mu}g/ml,\;and\;10{\mu}g/ml$ culture media. Those were Equiseti Herbs, Sophorae Subprostratae Radix, Moutan Radicis Cortex. Trichosanthis Radix, Buddleiae Flos. Cyperi Rhizoma. Benincasae Semen, Armeniacae Semen. Zedoariae Rhizoma, Astragali Semen, Dolichoris Semen. Lilii Bulbus, Asparagi Radix, Atractylodes Rhizoma White, Polygonati Officinallis Rhizoma. Conculusions: These findinga indicate that some oriental materia medica, specially Antipyretics, Herbs for Resolving Phlegm, Relieving Cough and Calming Wheezing and Herbs for Tonifring and Invigorating effects inhibit the splenocyte from secreting the IL-5. And further study experimented in vivo is needed for treating IL-5-driven inflammatory disease including asthma.

• Herbal Formula Science
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• v.26 no.1
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• pp.81-102
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• 2018

Objectives : I analysed daehwangmokdan-tang's compatibility principle by the system of chief, deputy, assistant, and envoy and investigated pharmacological activities by categorizing with chemical components, molecular level, cellular level, animal level and human level based on Korean and Chinese studies for this formula. Methods : Daehwangmokdan-tang's compatibiltity principle was examined by the system of chief, deputy, assistant, and envoy. I looked into studies that presented in Korea from 1956 to 2016 about daehwangmokdan-tang through KOREA INSTITUTE OF ORIENTAL MEDICINE, Korean medicine information system (OASIS) and in Chinese for 20 years about daehwangmokdan-tang through China National Knowledge Infrastructure, CNKI. Then classify into chemical components, molecular level, cellular level, animal level and human level to analyse. Results : According to the system of chief, deputy, assistant, and envoy, chief herb is Rhei Radix et Rhizoma and Moutan Cortex, deputy herb is Natrii Sulfas and Persicae Semen, assistant and envoy herbs are Trichosanthis Semen. The amount of extraction of paeonol, total anthraquinone, and conjugated anthraquinone from daehwangmokdan-tang with the formulation of the system of chief, deputy, assistant, and envoy was the highest, and in the formulation of chief herb and deputy herb, the extraction amount of paeonol and conjugated anthraquinone was the lowest. With other formulations, the amount of extraction of total anthraquinone and conjugated anthraquinone was improved, although the degree was different. In particular, when it is blended with Persicae Semen as a deputy herb, the extraction amount of total anthraquinone and conjugated anthraquinone of Rhei Radix et Rhizoma as a chief herb is greatly increased, and the extraction amount of paeonol is rather different, but it is lowered. It was found that the amount of Mg Ca K Na in daehwangmokdan-tang was the highest. Pharmacological activities can be detected in inflammatory mediators and enzymes for molecular level. For cellular level, it can be determined in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell line. In mouse and rats for animal level and human level, in inflammatory diseases (acute appendicitis, acute pancreatitis, acute cholecystitis, acute abdominal disease, ect.), pharmacological activities was caught. Conclusions : From the above results, daehwangmokdan-tang is composed in line with the system of chief, deputy, assistant, and envoy, suggesting that there is certain rationality and scientific. Pharmacological activities of daehwangmokdan-tang are effective to anti-inflammation, improvement of sepsis, analgesic, muscle relaxation, and improvement of intestinal flora and its metabolites. Daehwangmokdan-tang is consistent with the action of diuresis and anti-inflammation in terms of the content of elements, suggesting that there is action of purging fire and removing blood stasis, defecation detoxification, cooling blood and clearing heat and activating blood and dispersing stasis.

• Korean Journal of Pharmacognosy
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• v.45 no.2
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• pp.168-173
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• 2014

The aim of this study was to evaluate the anti-helicobacter activity of the ethanol extract of Sohamhyoongtang (Coptidis Rhizoma, Pinelliae Tuber and Trichosanthis Semen) and Coptidis Rhizoma total alkaloids, which is one of the components of Sohamhyoongtang. Crude ethanol extract of Sohamhyoongtang (ESHHT) and Coptidis Rhizoma total alkaloids (CRTA) were used for this experiment. Five different types of H. pylori (including H. pylori 26695) were used as test strain. To determine anti-helicobacter activity, minimum inhibitory concentration (MIC) was determined by agar dilution method. The effect of ESHHT and CRTA on the gene expression of H. pylori was investigated by quantitative realtime-PCR (qRT-PCR). MICs of ESHHT against five H. pylori strains were $250{\sim}500{\mu}g/ml$ and MICs of CRTA against five H. pylori strains were $50{\sim}200{\mu}g/ml$. Four representative virulence genes of H. pylori, cagA, ureA, ureB and ureI were tested as target genes for qRT-PCR. According to the qRT-PCR results, both ESHHT and CRTA markedly repressed the expression of cagA gene of H. pylori 26695 (6.91 and 20 folds respecively). These results showed that the ESHHT and CRTA demonstrated antihelicobacter properties, suggesting their potential use in gastritis or duodenal ulcer.