Study on the Antiinflammatory Effect of Gleditschiae Spina -Focussed on Takrisodokum and Kwaluubangtang-

조각자의 소염작용(消炎作用)에 대(對)한 실험적(實驗的) 연구(硏究) 부제(附題) : 탁이소독음(托裏消毒飮)과 과루우방탕(瓜蔞牛蒡湯)의 가감(加減)에 따른 효능비교(效能比較)

The inhibitory effects of Gleditschiae Spina, Cleditschia saponin, two complex prescriptions such as Takrisodokum and Kwaluubangtang, which contain Gleditschiae Spina, and their altered prescriptions on the activity of cyclooxygenase in vitro were investigated. The water extract of Gleditschiae Spina and Gleditschia saponin markedly inhibited the cyclo-oxygenase activity by 59% and 67%, respectively, at a concentration of $1mg/m{\ell}$, on the other hand, Takrisodokum and Kwaluubangtang showed 15% and 7% inhibition, respectively. The extracts with the substraction of Gleditschiae Spina and/or Platycodi Radix inhibited to a much smaller extent than Takrisodokum. However, in case of the extracts with the substraction of Gleditschiae Spina and/or Trichosanthis Semen, only one prescription, which does not contain Trichosanthis Semen, exhibited the same inhibition as Takrisodokum. From above results, Gleditschiae Spina was found to be most important for the effect of Takrisodokum and Kwaluubangtang on their antiinflammatory action. Instead of Gleditschiae Spina, another parts of this plant were added to both complex prescriptions and tested according to above methods, indicating that Gleditschiae Spina is more important than another parts for both prescriptions. When the administered dose of Gleditschiae Spina is increased(twofold and threefold) in both complex prescriptions, the inhibitory effect in Takrisodokum was diminished. however, the effect in Kwaluubangtang was increased.