• 미생물학회지
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• 제48권4호
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• pp.305-308
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• 2012

본 연구는 편백나무 건조 중 발생하는 휘발성 유기화합물로 구성된 응축수에 대해 항진균 및 항산화 활성을 평가하였다. 응축수는 hexane과 ethyl acetate로 각각 추출하였으며 추출액의 농도 100-500 mg/L에서 피부사상균에 대한 항진균 활성을 측정하였다. 추출액 농도가 증가할수록 항진균 효과도 증가하였으며 500 mg/L에서는 모든 피부사상균에 대해 높은 항진균 활성을 나타냈다. 추출용매에 따라서는 hexane으로 추출하였을 경우 높은 활성을 나타냈으며, 특히 Trichophyton rubrum에 대한 항진균 활성이 높았다. 항산화 활성을 측정한 결과 25 mg/L 이상의 농도에서는 90% 이상의 항산화 활성을 나타냈으며 추출용매에 따른 차이는 거의 없었다. 하지만 25 mg/L 이하의 농도에서는 hexane 추출물에서 높은 항산화 활성을 나타냈다. 각각의 추출물 구성성분을 분석한 결과 hexane 추출물의 주요 구성성분은 torreyol, alpha-cadinol, tau-cadinol이며, ethyl acetate 추출물의 주요 구성성분은 alpha-amorphene, alpha-cadinol, gamma-cadinene으로 나타났다.

• 생명과학회지
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• 제9권1호
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• pp.63-68
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• 1999

5-halogen substituted uridine, amino acid, peptide 및 penicillin G의 5'-amino -5' -deoxyuridine con-jugates, 5'-monophosphate uridine 유도체, 5'-monophosphate uridine -fat쇼 acid 유도체와 같은 nu-cleoside 화합물들을 화학적으로 합성한 후 이들의 항진균, 항균 및 항암 활성을 측정하였다. 5-Bromo-2',3'-O-isopropylideneuridine(6)은 Trichophyton rubrum의 성장을 억제하였다(MIC: $0.2{\mu}$g/ml). 5'-Amino-5' -deoxyuridine -penicillin G(19), 5'-amino-5'-deoxyuridine-cyclo(Phe -Asp)(20), 5-iodo-5'-amino -5' -deoxyuridine-penicillin G(22)는 항균적이었고(S. aureus에 대해 MIC가 $6.25{\mu}$g/ml) 뒤의 두 nucleoside 화합물은 항암 작용이 가장 강한 유도체이었다(L5178Y murine lymphoma cell에 대한 $IC_{50}$이 $6.5{\mu}$g/ml).

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2001년도 춘계학술발표논문집
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• pp.18-25
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• 2001

A new wood-degrading fungus Trichophyton rubrum LKY-7 secretes a high level of laccase in a glucose-peptone liquid medium. The production of laccase by the fungus was barely induced by 2,5-xylidine. The laccase has been purified to homogeneity through three chromatography steps in an overall yield of 40%. The molecular mass of the purified laccase was about 65 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified laccase had the distinct blue color and had basic spectroscopic features of a typical blue laccase: two absorption maxima at 278 and 610 nm and a shoulder at 338 nm. The N-terminus of the laccase has been sequenced, revealing high homology to laccases from wood-degrading white-rot fungi such as Ceriporiopsis subvermispora. The enzyme had a "low" redox potential (0.5 V vs normal hydrogen electrode), yet it was one of the most active laccases in oxidizing a series of representative substrates/mediators. Compared with other fungal laccases, the laccase has a very low Km value with ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid] as a substrate and a very high Km value with violuric acid as a substrate. The laccase has the isoelectric point of 4.0. The laccase had very acidic optimal pH values (pH 3-4) while it was more stable at neutral pH than at acidic pH. The laccase oxidized hydroquinone faster than catechol and pyrogallol. The oxidation of tyrosine by the laccase was not detectable under the reaction conditions. The laccase was strongly inhibited by sodium azide and sodium fluoride. fluoride.

• Journal of Yeungnam Medical Science
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• 제8권2호
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• pp.45-51
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• 1991

저자는 우리나라에서 아직 보고되어 있지 않지만 앞으로 유입될 가능성이 있는 T. tonsurans에 대하여 경험을 넓히고자 미국에서 환자에서 분리한 T. tonsurans에 대상으로 여러가지 진균학적 검사를 실시하여 다음과 같은 결과를 얻었다. 1. 육안적으로 표면은 편평하고 무두질한 양가죽 양상으로 미세한 과립이 얇게 덮혀있고 연한 회색 또는 연한 황색을 나타내었으며 중앙에는 굴곡이 있고 뒷면은 마호가니 갈색을 나타내었다. urease test에서 균주에 따라 다양한 색깔을 나타내었다. 2. 현미경 소견에서 격벽이 있는 균사를 비롯하여 대분생자, 소분생자, spiral hyphae, 후막포자가 발견되었으며 소분생자의 배열과 모양이 특징적으로 균사를 중심으로 양옆으로 배열되어 길고 그 끝이 성냥 끝처럼 부풀어 있었다. 3. 모발천공검사에서 19주중 16주에서 양성소견을 보였다.

• 펄프종이기술
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• 제36권5호
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• pp.69-77
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• 2004

Degradations of pine, yellow poplar and sweet gum by two fungi, Pycnoporus cinnabarinus and Trichophyton rubrum LSK-27 were investigated. P. cinabarinus degraded pine block samples much faster than T rub rum LSK-27, whereas P. cinnabarinus and T rubrum LSK-27 degraded yellow poplar and sweet gum at almost the same rate. In an effort to get a better understanding of how fungi degrade lignin in wood, contents of various functional groups were analyzed. After three-months of degradation of pine flour by these fungi, the following changes were observed: an increase in condensed phenolic OH group and carboxylic acid group content, a decrease in the guaiacyl phenolic OH content, and little change of aliphatic OH group content. Further studies in the degradation of pine flour by P. cinnabarinus indicated that the increase in condensed phenolic OH group content and the decrease in guaiacyl phenolic OH group content occurred in the first month of the degradation. The changes of functional group contents in the degradation of unbleached softwood kraft pulp by P. cinnabarinus had the same trends as those in the degradation of pine flour. That is, structural alteration of lignin due to the kraft pulping process had little effect on how P. cinnabarinus degraded lignin.

• 대한임상검사과학회지
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• 제39권2호
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• pp.91-95
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• 2007

The number of children patients with tinea pedis and onychomycosis diagnosed on clinical findings and culture at the Catholic Dermatological Clinic in Daegu City were 144,446 for 2 years from January 2005 to December 2006. 120 children were suspected of having clinical onychomycosis or tinea pedis. KOH smears and cultures on the Potato corn meal dextrose agars (PDCA) were done for the suspected toe webs, nails and uninvolved neighbor toe webs. Socks were dusted and cultured to check contamination of clothes. Family infections were checked by questionnaire. The incidence was 92 (0.06%) out of 144,446 outpatients in tinea pedis, 28 (0.02%) in onychomycosis. Trichophyton(T.) rubrums were isolated from all of the 7 cases of onychomycosis. T. mentagrophytes was isolated from 5 cases of the tinea pedis patients and T. rubrum was isolated from 50 (92.5%) cases of the tinea pedis. The right side was predominant for onychomycosis of both toe nails (23 of 25) and finger nails (3 of 3). No fungal elements were detected from normal looking neighbor toe webs by KOH examinations. However, culture on PDCA agars showed T. rubrum isolates from 3 toe webs. 7 showed positive cultures from the socks. From the questionnaire, 94 had family infections.

• Journal of Microbiology
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• 제45권5호
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• pp.460-465
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• 2007

This study was carried out in order to investigate the potential of using plant oils derived from Leptospermum petersonii Bailey and Syzygium aromaticum L. Merr. Et Perry as natural antifungal agents. The antifungal effects of essential oils at concentrations of 0.05, 0.1, 0.15, and 0.2 mg/ml on the dermatophytes Microsporum canis (KCTC 6591), Trichophyton mentagrophytes (KCTC 6077), Trichophyton rubrum (KCCM 60443), Epidermophyton floccosum (KCCM 11667), and Microsporum gypseum were evaluated using the agar diffusion method. The major constituents of the active fraction against the dermatophytes were identified by gas chromatography-mass spectrometry and high-performance liquid chromatography analysis. The antifungal activities of S. aromaticum oil (clove oil) against the dermatophytes tested were highest at a concentration of 0.2mg/ml, with an effectiveness of more than 60%. Hyphal growth was completely inhibited in T. mentagrophytes, T. rubrum, and M. gypseum by treatment with clove oil at a concentration of 0.2 mg/ml. Eugenol was the most effective antifungal constituent of clove oil against the dermatophytes T. mentagrophytes and M. canis. Morphological changes in the hyphae of T. mentagrophytes, such as damage to the cell wall and cell membrane and the expansion of the endoplasmic reticulum, after treatment with 0.11 mg/ml eugenol were observed by transmission electron microscopy (TEM). At a concentration of 0.2 mg/ml, L. petersonii oil (LPO) was more than 90% effective against all of the dermatophytes tested, with the exception of T. rubrum. Geranial was determined to be the most active antifungal constituent of L. petersonii oil. Taken together, the results of this study demonstrate that clove and tea tree oils exhibited significant antifungal activities against the dermatophytes tested in this study.

• 한국수산과학회지
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• 제48권4호
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• pp.432-438
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• 2015

This study examined the antimicrobial, antioxidant, and tyrosinase inhibitory activities of bioactive compounds extracted from two starfish, Asterina pectinifera and Asterias amurensis, using solvent extraction after $Protamex^{TM}$ hydrolysis. Methanol and acetone fractions collected by stepwise extraction from specimens were subjected to silica gel column chromatography (SGCC) (200 mesh and 400 mesh), followed by high-performance liquid chromatography (HPLC). Two fractions (7:3 and 5:5 chloroform : methanol ratio, v/v) eluted using silica gel column chromatography from the two starfishes showed higher antimicrobial activity against Propionibacterium acnes and dermatophyte fungi (Epidermophyton floccosum, Microsporum audouinii, Trichophyton ferrugineum, Trichophyton mentagrophytes, and Trichophyton rubrum), antioxidant activity ($EDA_{50}$, mg/mL), and tyrosinase inhibitory activity compared to the other fractions. The final fractions obtained from Asterina pectinifera (RT 7.53, 8.93, and 10.48 min) and Asterias amurensis (RT 5.02 min) by SGCC (400 mesh) and HPLC from two SGCC fractions (200 mesh) showed 8.94 and 15.59 mg/mL antioxidant activity ($EDA_{50}$) and 46.89 and 40.19 % tyrosinase inhibitory activity, respectively. Extracts from starfishes are potential cosmetic basic material.

• 생명과학회지
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• 제19권1호
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• pp.94-100
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• 2009

GSE와 PSS를 이용한 피부 외용제를 개발하기 위해, 평판 배지확산법 및 액체배지감수성실험을 이용하여 GSE와 PSS의 비듬균(Malassezia furjur, M. restricta), 여드름균(Propionibacterium acnes) 및 무좀균(Trichophyton mentagrophytes, T. rubrum)에 대한 항균 활성을 측정하였다. 평판 배지확산법의 경우 GSE가 $10{\mu}l/disk$일 때 M. furfur, M. restricta. P. acnes, T. mentagrophytes 및 T. rubrum은 각각 1.0, 1.0, 12.0, 15.0, 15.0 mm 였으며, PSS가 $10{\mu}l/disk$일 때에는 각각 7.3, 5.7, 2.0, 0 mm 이었다. 또한 액체배지감수성실험에서 GSE에 대한 MIC 값은 각각 3.91, 3.91, 0.004, 0.024, $0.012{\mu}l/ml$ 이었으며 PSS에 대한 MIC 값은 각각 0.03, 0.03, 0.156, 0.003, $0.012{\mu}l/ml$이었다. 또한 GSE 와 PSS가 함유된 에멀전 시제품을 제조하여 농도별 평판배지확산법을 수행한 결과 그 결과 값은 0.5% 농도에서 각각 5.2, 4.3, 8.0, 9.5, 12.8 mm의 값을 나타내었다. GSE 와 PSS가 실험 대상 균주에 대하여 모두 항균력을 가지고 있었으며 비듬균인 M. furfur와 M. restricta는 GSE 보다는 PSS에서 더 강한 항균력을 보였으며 여드름균인 P. acnes와 무좀균인 T. mentagrophytese 및 T. rubrum은 PSS 보다는 GSE에서 더 강한 항균 활성을 보였다. 그러므로 GSE와 PSS의 각각에 효과적인 균주별 피부질환 외용제 뿐만 아니라 GSE와 PSS를 함께 적용한 광범위한 피부질환 외용제의 응용개발이 가능하리라 사료된다. 또한 에멀전 로션 시제품의 물리화학적 특성을 분석한 결과 pH, 점도, 굴절 및 색도 변화에 있어서 모두 안정성을 보였고 이상의 연구결과를 바탕으로 기능성 화장품과 같은 피부 외용제 제품의 개발 가능성을 확인하였다.

• 대한임상검사과학회지
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• 제40권2호
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• pp.80-85
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• 2008

Microsporum gypseum is a geophilic fungus abundant in soil throughout the world. M. gypseum has been identified for the first time in Korea at 1976. The purpose of this study was to investigate epidemiologic aspects of M. gypseum. We performed the epidemiologic study an 198 patients with M. gypseum infections in from January 1976 to December 2005. From 100,012 patients, dermatophytes were isolated as follows; Trichophyton rubrum was isolated from 86,553 (86.5%), T. interdigitale 3,991 (4.0%), Epidermophyton floccosum 1.021 (0.1%), T. tonsurans 141 (0.1%). T. mentagrophytes 3.930 (3.9%), T. verrucosum 220 (0.2%), Microsporum canis 3.879 (3.9%), and M. gypseum 198 (0.2%). Number of patients with M. gypseum by site and age were as follows; most patients infected with tinea corporis were young people under 10 years. M. gypseum were most frequently isolated in summer and autumn.