• Title/Summary/Keyword: Trichogramma dendrolimi

Search Result 3, Processing Time 0.017 seconds

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis (ITS2 rDNA 염기서열 분석을 통한 Trichogramma 속(벌목: 알벌과)의 조명나방 알기생벌에 대한 종 추정)

  • Seo, Bo Yoon;Jung, Jin Kyo;Park, Ki Jin;Cho, Jum Rae;Lee, Gwan-Seok;Jung, Chung Ryul
    • Korean journal of applied entomology
    • /
    • v.53 no.3
    • /
    • pp.247-260
    • /
    • 2014
  • To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3'terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ${\leq}0.005$ and ${\geq}0.080$, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

Effects of Photoperiod on Oviposition and Emergence of Egg Parasitoid, Trichogramma dendrolimi Mastumura (Hymenoptera, Trichogrammatidae) (광조건이 송충알벌(벌목, 알벌과)의 산란과 우화에 미치는 영향)

  • 박영규;이해풍;이기상;한만위;이정운
    • Korean journal of applied entomology
    • /
    • v.38 no.2
    • /
    • pp.93-99
    • /
    • 1999
  • We investigated the effect of constant photoperiod (16L : 8D, 12L : 12D, 8L : 16D), on the oviposition, parasitism, and emergence of Trichogramma dendrolimi. Also during 16L : 8D condition circadian, oviposition and emergence of this species on artificial eggs were checked every 2hrs. When twelve eggs of Antheraea pern~i and artificial eggs were provided, the wasps had the highest rate of oviposition in the first day, and decreased dramatically there after. In these different photoperiod, the total oviposition numbers per A. pernyi eggs by the wasp were 161.8 in 16L : 8D, 145.8 in 121 : 12D and 128.5 in 8L : 16D respectively in the first day. On the other hand for the artificial egg in 16L : 8D photoperiod, the total oviposition number per egg was 106.8 in the first day, 26.6 in the 2nd day and 21.3 in the 3rd day. The rate of emergence from A. pernyi eggs was not different depend on wasp's day age, however in the artificial eggs, after the first day's 84.7% decreased considerably. The emergence rate from artificial eggs was the highest comparatively between 04:OO-0600 hours.

  • PDF

Studies on a Korean Unrecorded Pamphilid -Sawfly (Jymenoptera, Symphyta) Feeding on Korean Pine(III) The Natural Enemies (잣남를 식해하는 한국미기록종 넓적잎벌 (막혈목, 광요아목) 에 관한 연구(III) 천적에 대하여)

  • 이덕상
    • The Korean Journal of Zoology
    • /
    • v.6 no.1
    • /
    • pp.21-24
    • /
    • 1963
  • 본 논문은 경기도 광릉시험림에서 잣나무 넓적잎벌의 천적에 의해서 1958-1961년에 조사연구한 것을 보고하는 것이다. 잣나무 넓적잎벌의 천적은 다음과 같다. 1. 난을 침해하는 것 : Trichogramma dendrolimi MATSUMURA 송충알사리벌 2. 수상의 유충을 침범하는 것 : Phora egregia BRUES 벼룩파리 (신칭)..... 미기록과속종 Sarcophaga albiceps MEIGEN 알비쉬파리(신칭) Streptopelia o. orientalis(LATHAM) 맷비둘기 3. 지중의 전용을 침해하는 것 : Paratrechina flavipes (f. SMITH) 불개미 Myrmica kurokii FOREL 비살헐개미(신칭) ... 미기록종 A Species of Acarina (진득이의 일종) A Species of NEmatoda (선충이 일종) Isaria sp. (경화균의 일종)

  • PDF