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http://dx.doi.org/10.5656/KSAE.2014.06.0.012

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis  

Seo, Bo Yoon (Crop Protection Division, National Academy of Agricultural Science, Rural Developmental Administration)
Jung, Jin Kyo (Crop Environment Research Division, National Institute of Crop Science, Rural Developmental Administration)
Park, Ki Jin (Maize Experiment Station, Gangwondo Agricultural Research and Extension Services)
Cho, Jum Rae (R&D Coordination Division, Research Policy Bureau, Rural Developmental Administration)
Lee, Gwan-Seok (Crop Protection Division, National Academy of Agricultural Science, Rural Developmental Administration)
Jung, Chung Ryul (Molecular biology Lab, Department of Biology, Kyungsung University)
Publication Information
Korean journal of applied entomology / v.53, no.3, 2014 , pp. 247-260 More about this Journal
Abstract
To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3'terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ${\leq}0.005$ and ${\geq}0.080$, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.
Keywords
Ostrinia furnacalis; Zea mays; Trichogramma; ITS2; Molecular phylogenetic tree;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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