• The Plant Pathology Journal
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• v.34 no.3
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• pp.171-181
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• 2018

Fruit rot is the principal phytopathological problem of pipiana pumpkin (Cucurbita argyrosperma Huber) in the state of Guerrero. The aims of this research were to 1) identify the causal agent of southern blight on pumpkin fruits by morphological, pathogenic, and molecular analysis (ITS1, 5.8S, ITS2); 2) evaluate in vitro Trichoderma spp. strains and chemical fungicides; and 3) evaluate under rainfed field conditions, the strains that obtained the best results in vitro, combined with fungicides during two crop cycles. Number of commercial and non-commercial fruits at harvest, and seed yield ($kg\;ha^{-1}$) were registered. Morphological, pathogenic and molecular characterization identified Sclerotium rolfsii as the causal agent of rot in pipiana pumpkin fruits. Now, in vitro conditions, the highest inhibition of S. rolfsii were obtained by Trichoderma virens strain G-41 (70.72%), T. asperellum strain CSAEGro-1 (69%), and the fungicides metalaxyl (100%), pyraclostrobin (100%), quintozene (100%), cyprodinil + fludioxonil (100%), and prochloraz (100%). Thiophanate-methyl only delayed growth (4.17%). In field conditions, during the spring-summer 2015 cycle, T. asperellum strain CSAEGro-1 + metalaxyl, and T. asperellum + cyprodinil + fludioxonil, favored the highest number of fruits and seed yield in the crop.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.288-293
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• 2010

'Cham', a new cultivar of Agrocybe aegerita, was bred by crossing between monokaryotic strains isolated from GMAG45021 and 'Beodeulsongi1ho' in Mushroom Research Station, Gyonggi Province A.R.E.S. in 2007. The optimum temperature for the mycelial growth of 'Cham' was 26 to $28^{\circ}C$ on PDA medium, whereas that of 'Beodeulsongi1ho'(control) was 24 to $26^{\circ}C$. The optimum temperature for the primordial formation and fruiting body development of 'Cham', as well as 'Beodeulsongi1ho', was 18 to $20^{\circ}C$. In the bottle cultivation, the spawn run period of 'Cham' at 22 to $23^{\circ}C$ was around 38 days and the period from scratching of inoculum to harvest was 12 days. These characteristics of 'Cham' were not different from those of 'Beodeulsongi1ho'. However, 'Cham' had more dark brown-colored, and thicker and stronger pileus, and longer and thicker stipe compared to that of 'Beodeulsongilho'. Freshness of 'Cham' was maintained for 10 days at the storage temperature of $4^{\circ}C$, while that of 'Beodeulsongi1ho' was maintained for 8 days. Moreover, the yield of fruiting bodies of 'Cham' was $141g/850m{\ell}$ bottle, which was similar to that of 'Beodeulsongi1ho'. Both 'Cham' and 'Beodeulsongilho' have lower resistivity against Trichoderma virens and Trichoderma harzianum.

• Journal of Mushroom
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• v.2 no.2
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• pp.69-75
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• 2004

This study was carried out to investigate effect of $CaCO_3$ treatment on cultivation of oyster mushroom for suppression of green mold disease and for promotion of mycelial growth to stabilize mushroom production in field and laboratory experiment. Treatment of $CaCO_3$ in PDA media promoted mycelial growth of mushroom and suppressed that of green mold. Addition of $CaCO_3$ in rice straw substrate increased mushroom mycelial growth compared with control. In that case, growth of green mold increased up to treated 0.6% $CaCO_3$ but decreased in treatment beyond 0.8% $CaCO_3$. There were some differences on effect of $CaCO_3$ treatment according to green mold species. Trichoderma longibrachiatum was effected but T. virens was not effected by treated $CaCO_3$. Differences among mushroom strains by treated $CaCO_3$ were not shown. It is confirmed that treatment of $CaCO_3$ can promote mushroom mycelial growth but it's not clear in the field. In the result of field test, treatment of $CaCO_3$ in rice straw substrates tended to increase yield and decrease incidence of disease compared with non-treatment. These results suggest that $CaCO_3$ treatment on cultivation of oyster mushroom can be applied to take preventive steps against of green mold disease.

• The Korean Journal of Mycology
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• v.29 no.2
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• pp.79-85
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• 2001

A strain of Dictyophora echinovolvata ASI 32002 showing good fruiting body formation was selected. Analyses of chemical and nutritional components as well as antimicrobial activity of different parts of the mushroom such as mycelium, egg, and fruiting body were carried out. There were differences in the chemical compositions and the quantities depending on developmental stages of veiled lady mushroom, D. echinovolvata ASI 32002. Nitrogen, phosphate, magnesium, and calcium in inorganic chemicals were abundant in mycelium, and potassium and mineral elements were abundant in the egg and fruiting body. Mannitol and trehalose were abundant in free sugar contents. Glutamic acid and arginine in mycelium and aspartic acid and glutamic acid in egg and fruiting body were abundant in free amino acid contents. Linoleic acid, an polyunsaturated fatty acid, was abundant in all parts of the Dictyophora species, but compositions and quantities of other fatty acids varied depending on the different parts of the mushroom. It was detected that malic acid, lactic acid and acetic acid in mycelium, formic acid, acetic acid and fumaric acid in egg, and malic acid, citric acid, lactic acid, fumaric acid in fruiting body were abundant. The methanol extracts of D. echinovolvata ASI 32002 mycelium showed antifungal activity with minimal inhibition concentration (MIC) of $62{\sim}125\;{\mu}g/ml$ that was similar levels of cyclohexamide against Aspergillus awamori, Hypocrea nigricance and Trichoderma virens. The MIC of extracts from mycelium and fruiting body against Candida albicans was $250\;{\mu}g/ml$, similar to that of tetracycline. In addition to the above results, further as food additives and ingredient of cosmetics.