• Korean Journal of Oriental Medicine
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• v.12 no.1
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• pp.23-38
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• 2006

The taxonomic list of specific features in external and internal shape and the pattern analysis of Aconitum carmichalei $D_{EBX}$ as the original plant of Aconiti Lateralis Radix Preparata and Aconitum cliiare Dc as the original plant of Aconiti Ciliare Tuber are as follows. 1. Aconitum carmichalei $D_{EBX}$ has tri-palmately parted leaves, petiole in lower leaves, and its ovary has short hair. Whereas Aconitum cliare Dc has $3{\sim}4$ parted leaves, long petiole, and its ovary has not hair. 2. Aconitum carmichalei $D_{EBX}$ has cylinder shape is relatively small in length and diameter, is greyish brown blacky brown in outer surface, greyish $white{\sim}dark$ gray in section. 3. According to the collection place, there is a remarkable difference in the physical shape of herbal states. Aconiti Lateralis Radix Preparate(medicated in Korea) is more transparent blacky brown color than Aconiti Lateralis Fadix Preparata(medicated in Chian). Also Black Aconi Radix(墨附片) has exodermis and White Aconi Radix(白附片) has not. 4. The internal characteristics entirely correspond to in internal shape described in the literatures, Only it is possible to discriminate between black Aconi Radix(墨附片) and White Aconi Radix(白附片) by the existence of cork layer. The classification between Aconiti Lateralis Radix Preparata and Aconiti Ciliare Tuber makes entirely Tuber makes entirely remarkable difference in the physical shape of cambium layer Namely, in shape of cambium layer the kinds of Aconiti lateralis Radix Prepala has horn-like shape and the kinds of Aconiti Ciliare Tuber has circle-like shape. 5. In the peak of examination substance in comparison to Rt of the index material diterpene alkaloid mesaconitine, aconitine, hypaconitine chromatogram Aconiti Ciliare Tuber is higher than in Aconiti Lateralis Radix Preparata This explain that the component changes after the process of medicine. 6. In the Content of mesaconitine, aconitine and hypaconitime Aconiti Ciliare Tuber is higher than Aconiti Lateralis Radix Preparata. 7. In Aconiti Lateralis Radix Preparata, aconitine, hypaconitine and mesaconitine each appears in Rf 0.46, 0.54, 0.32. But except Aconiti Ciliare Tuber the band does not appear. For the future, such results will be used as the basic source of additional research, and a far-reaching comparative study is needed to distinguish between many kinds of same genus-degree of relatedness.

• Tuberculosis and Respiratory Diseases
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• v.48 no.1
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• pp.24-32
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• 2000

Purpose: Microsatellite instability(MSI) is frequently used as an indicator of microsatellite mutator phenotype(MMP) tumors. MSI has been observed in a percentage of non-small cell lung cancer(NSCLC). However, its role in tumorigenesis of NSCLC remains unknown. The frequency and pattern of MSI in NSCLC were evaluated and clinical parameters of MSI-positive tumors with those of MSS(microsatellite stable) tumors were compared. Materials and Methods: Twenty surgically resected NSCLCs were analyzed for 15 microsatellite markers located at chromosomes 3p and 9p. The peripheral blood lymphocytes of patients were used as the source of the normal DNA. Results: 1) Of 20 cases, 8(40%) demonstrated MSI. 2) Instability was observed more frequently in tri- and tetra-nucleotide repeats than in dinucleotide repeats. In all cases, instability appeared as a shift of individual allelic bands. 3) LDH was observed in 10(50%) of 20 tumors analyzed. 4) Of 20 cases, MSI-H tumor(showing MSI in the majority of markers) was absent. There were 5 MSI-L tumors(showing MSI in a greater than 10% of markers). 5) No significant difference was observed between MSI-L tumors and MSI-negative tumors in terms of clinicopathologic features such as pack-year history of smoking, histologic subtype, and(delete) stage of disease. There was also no significant difference in the incidence of LDH in relation to the status of MSI. Conclusion: These data strongly suggest that MSI plays different roles in lung and colon cancer. MMP pathway appears to be far less important in the tumorigenesis of NSCLC, caused mainly by cigarette smoke, with little familial tendency.