• Korean Journal of Microbiology
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• v.38 no.4
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• pp.209-209
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• 2002

Mycobacterium sp. strain JCl DSM 3803 grown in methanol showed no methanol dehydrogenase or oxidase activities found in mast methylotrophic bacteria and yeasts, respectively. Even though the methanol-grown cells exhibited a little methanol-dependent oxidation by cytochrome c-dependent methanol dehydrogenase and alcohol dehydrogenase, they were not the key enzymes responsible for the methanol oxidation of the cells, in that the cells contained no c-type cytochrome and the methanol oxidizing activity from the partially purified alcohol dehydrogenase was too low, respectively. In substrate switching experiments, we found that only a catalase-peroxidase among the three types of catalase found in glucose-grown cells was highly expressed, in the methanol-grown cells and that its activity was relatively high during the exponential growth phase in Mycobacterium sp. JCl. Therefore, we propose that catalase-peroxidase is an essential enzyme responsible for the methanol metabolism directly Of indirectly in Mycobacterium sp. JCl.

• Journal of the Korea Institute of Building Construction
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• v.12 no.3
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• pp.347-357
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• 2012

While it is generally possible to install curved panels manufactured in a factory within the permitted error range on an irregular surface frame of concrete or steel, it is difficult and expensive. Freeform architecture is thus designed and constructed differently from formal buildings. In order to more easily and inexpensively actualize freeform architecture, Building Information Modeling (hereinafter referred to as BIM) has recently been applied in the construction industry. However, the related research and case analyses are not sufficient to identify the implications and contributions of freeform buildings in future similar projects. Therefore, this research will study design and construction methods for freeform surfaces, particular the concrete surface frame of freeform buildings based on BIM, focused on the Tri-Bowl project. This study attempts to analyze the pros and cons of each method for the concrete surface frame of the Tri-Bowl, and then presents the lessons learned and implications related to the design and construction process of the freeform architecture. Several implications for design and construction of concrete surface frame of the freeform building, the Tri-Bowl, are found. The first is that manufacturing and installation of a curved concrete frame is precisely performed based on the exact numerical values of materials and installation made using BIM 3D technologies, such as CATIA and Rhino. The second is that close and continuous collaboration among the different participants in the construction of the Tri-Bowl allowed them to cope with virtual conditions. The third is that design and construction processes have changed, and high quality of the surface frame of a freeform building is required.

• Journal of the Korean Society of Propulsion Engineers
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• v.25 no.1
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• pp.19-28
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• 2021

In rotating detonation engine(RDE), only the detonation wave is moving around the outer wall of the combustor. Neither a mechanical part nor flow is rotating in RDE. Thus, the RDE cross section is not necessary to be circular, but arbitrary closed section is possible. A RDE of tri-arc cross section is designed and As an example of an arbitrary cross sectioned RDE, a RDE of tri-arc cross section is designed in this study, and operational and performance characteristics were examined experimentally. The rotation of the detonation wave is confirmed by dynamic pressure sensor and high-speed camera, while the characteristics of the detonation wave were investigated at the concave and convex surfaces. In the present study, the thrust level of 17.0 N to 96.0 N was obtained depending on the mass flow rate.

• Journal of the Korean Wood Science and Technology
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• v.42 no.6
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• pp.740-746
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• 2014

The unripe fruit of cudrania tricuspidata was extracted with 50% ethanol. The crude water-soluble extracts were separated by liquid-liquid separation with n-hexane, ethyl acetate and butanol followed by precipitation with ethanol, and then the water-soluble polysaccharide (F1) was isolated by the fractionation through gel permeation chromatography using preparative PLaquagel-OH column with water. The structure was characterized by monosaccharide composition with HPAEC-PAD, methylation analysis with GC-MS, FT-IR and HPLC. According to the data, F1 was com posed of glucose (22.84 mM), galactose (13.75 mM), arabinose (45.87 mM), xylose (7.49 mM). It was revealed which uronic acid and acetyl group were not attached in F1. And it is constituted of 1-linked arabinose, 1,4-linked arabinose, 1,3-linked glucose, 1,4-linked galactose, 1,4-linked glucose, 1,3,6-linked galactose, 1,3,6-linked glucose and the ratio was showed 1.1 : 1.0 : 4.9 : 7.5 : 3.0 : 3.1 : 1.4 : 1.5.

• Journal of Sensor Science and Technology
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• v.25 no.2
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• pp.79-85
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• 2016

This paper proposes a threshold-based fall recognition algorithm to discriminate between falls and activities of daily living (ADL) using a tri-axial accelerometer and a bi-axial gyroscope sensor mounted on the upper sternum. The experiment was executed ten times according to the proposed experimental protocol. The output signals of the tri-axial accelerometer and the bi-axial gyroscope were measured during eight falls and eleven ADL action sequences. The threshold values of the signal vector magnitude (SVM_Acc), angular velocity (${\omega}_{res}$), and angular variation (${\theta}_{res}$) parameter were calculated using MATLAB. From the preliminary study, three thresholds (TH1, TH2, and TH3) were set so that the falls could be distinguished from ADL. When the parameter SVM_Acc is greater than 2.5 g (TH1), ${\omega}_{res}$ is greater than 1.75 rad/s (TH2), and ${\theta}_{res}$ is greater than 0.385 rad (TH3), these action sequences are recognized as falls. If at least one or more of these conditions is not satisfied, the sequence is classified as ADL.

• Journal of the Korean Society of Safety
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• v.13 no.2
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• pp.109-115
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• 1998

Tri-cresyl phosphate(TCP) was synthesized by reaction of phosphorus chloride with mixed cresol(mixture of m-cresol, p-cresol, and others) in the presence of $AlCl_3$. Some of unwanted products and unreactants colored TCP. In order to separate TCP from these, vaccume distillation was carried twice, but colorless TCP could not be producted. Separation of unwanted materials by 2% NaOH solution was introduced before first and second distillation and optimal separation conditions such as NaOH concentration, mixing volume ratio, mixing time, and rpm were investigated for new batch separation and production of colorless TCP Optimal conditions were 2% NaOH solution, 35% mixing volume ratio of 2% NaOH solution, 1.5 hours of mixing time, and 20 rpm.

• Journal of environmental and Sanitary engineering
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• v.14 no.3
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• pp.90-98
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• 1999

This study find out the effect of red Ginseng extract(1.0g/kg) against TBTO(10, 20 and 40mg/kg) poisoning on each organs, hematological, serum biochemistry in rat for 3 weeks. 1. Each organ weight per body weight ration in treated group, all rats liver were significantly increased. (P<0.05,P<0.01)2. Iin TBTO treated group, the hematological parameters such as WBC, RBC, Hgb, Hct, PLt, MCV, MCH and MCHC in serum were remarkably decreased in comparison to that of control group. Only added red Ginseng extract group were slightly decreased but not significanted. 3. In TBTO treated group, the biochemical parameters such as AST, ALT in serum were remarkable elevated in comparison to that control group (P>0.05).4. In TBTO treated group, the activity of Triglyceride in serum of male rat were significantly increased in comparison to that of control group (P<0.05, P<0.01).5. In case of B.U.N activity in treated group(10, 20 and 40 mg/kg) were almost increased in comparison to that of control group, but not added red Ginseng extract.

• The Journal of Korean Physical Therapy
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• v.22 no.6
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• pp.59-63
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• 2010

Purpose: Driving a car is an essential component of daily life. For safe driving, each driver must perceive sensory information and respond rapidly and accurately. Brake response time (BRT) is a particularly important factor in the total stopping distance of a vehicle, and therefore is an important factor in traffic accident prevention research. The purpose of the current study was (1) to compare accelerometer. BRTs analyzed by three different methods and (2) to investigate possible correlations between accelerometer-BRTs and foot switch-BRTs, which are measured method using a foot switch. Methods: Eighteen healthy subjects participated in this study. BRT was measured with either a tri-axial accelerometer or a footswitch. BRT with a tri-axial accelerometer was analyzed using three methods: maximum acceleration time, geometrical center, and center of maximum and minimum acceleration values. Results: Both foot switch-BRTs and accelerometer-BRTs were delayed. ANOVA for accelerometer BRTs yielded significant main effects for axis and analysis, while the interaction effect between axis and analysis was not significant. Calculating the Pearson correlation between accelerometer-BRT and foot switch-BRT, we found that maximum acceleration time and center of maximum and minimum acceleration values were significantly correlated with foot switch-BRT (p<0.05). The X axis of the geometrical center was significantly correlated with foot switch-BRTs (p<0.05), but Y and Z axes were not (p>0.05). Conclusion: These findings suggest that the maximum acceleration time and the center of maximum and minimum acceleration value are significantly correlated with foot switch-BRTs.

• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.933-938
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• 2006

Decaprenyl diphosphate synthase (DPS) is the key enzyme for the production of coenzyme $Q_{10}$ ($CoQ_{10}$). A dps gene from Sinorhizobium meliioti KCCM 11232 (IFO 14782) was isolated by PCR and then cloned in Escherichia coli. DNA sequencing analysis revealed an open reading frame of 1,017 bp encoding a 338-amino-acid protein. The protein was identical at the 98% level to the putative octaprenyl diphosphate synthase (IspB) of S. meliloti 1021. The deduced amino acid sequence included the DDxxD domains conserved in the majority of the prenyl diphosphate synthases. Heterologous expression in E. coli BL21 (DE3) was carried out, and the $CoQ_{10}$ produced was then analyzed by HPLC. E. coli BL21 (DE3) harboring the dps gene from S. melioti produced CoQ$_{10}$ in addition to endogenous coenzyme Q$_8$ (CoQ$_8$), whereas wild-type E. coli BL21 (DE3) host did not have the ability of producing CoQ$_{10}$. The results suggest that the putative dps from S. meliloti KCTC 2353 encoded the DPS.

• Journal of the Korean Chemical Society
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• v.23 no.3
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• pp.175-179
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• 1979

In acidic media such as aqueous acetic acid, phthalimide is reduced with zinc dust to give 3-hydroxyphthalimidine while the 2,2,2-trichloroethyl esters or glycosides are reductively cleaved. However, it has been discovered that, by employing a mixture of THF and pH 4.5 buffer solution as a solvent, 2,2,2-trichloroethyl group can be selectively removed with activated zinc dust in the presence of phthalimido function, provided that the reactant or the product does not have any free carboxylic acid function. By applying the above methods, reaction of $2,2,2-trichloroethyl 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-{\beta}-D-glucopyranoside$ (1) with activated zinc dust gave a good yield of $3,4, 6-tri-O-acetyl-2-deoxy-2-phthalimido-{\beta}-D-glucopyranose$ (5) in THF-buffer solution, and $3,4,6-tri-O-acetyl-2-deoxy-2-(3-hydroxyphthalimidino)-{\beta}-D-glucopyranose$ (6) in aqueous acetic acid.