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http://dx.doi.org/10.5658/WOOD.2014.42.6.740

Structure Analysis of Water-soluble Polysaccharides Extracted from The Unripe Fruit of Cudrania tricuspidata  

Kim, Seok Ju (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
Lee, Kyung-Tae (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
Youe, Won-Jae (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
Lee, Sung-Suk (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
Kim, Yong Sik (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
Publication Information
Journal of the Korean Wood Science and Technology / v.42, no.6, 2014 , pp. 740-746 More about this Journal
Abstract
The unripe fruit of cudrania tricuspidata was extracted with 50% ethanol. The crude water-soluble extracts were separated by liquid-liquid separation with n-hexane, ethyl acetate and butanol followed by precipitation with ethanol, and then the water-soluble polysaccharide (F1) was isolated by the fractionation through gel permeation chromatography using preparative PLaquagel-OH column with water. The structure was characterized by monosaccharide composition with HPAEC-PAD, methylation analysis with GC-MS, FT-IR and HPLC. According to the data, F1 was com posed of glucose (22.84 mM), galactose (13.75 mM), arabinose (45.87 mM), xylose (7.49 mM). It was revealed which uronic acid and acetyl group were not attached in F1. And it is constituted of 1-linked arabinose, 1,4-linked arabinose, 1,3-linked glucose, 1,4-linked galactose, 1,4-linked glucose, 1,3,6-linked galactose, 1,3,6-linked glucose and the ratio was showed 1.1 : 1.0 : 4.9 : 7.5 : 3.0 : 3.1 : 1.4 : 1.5.
Keywords
Cudrania tricuspidata unripe fruit; water-soluble polysaccharides; Gel Permeation Chromatography; Gas Chromatography-Mass Spectroscopy; methylation analysis;
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