• Korean Journal of Veterinary Service
• /
• v.16 no.1
• /
• pp.51-56
• /
• 1993

Feces were collected from pigs of various ages on slaughter house and swinery in the southern area of the kangwon-do. Treponema hyodysenteriae were isolated from the feces by the method of Jenkinson. and the effects of temperature on the survival of these organism were investigated. The results abtained are summerized as follows ; 1. T. hyodysenteriae were isolated from 22 fecal samples of 1, 296 samples, and showed higher incidence at 4-8week age groups than other age groups. 2. The organisms were isolated from 17 samples of 329 diarrhea samples and 5 samples of 967 non-diarrhea samples. 3. T hyodysenteriae survived long at $0-10^{\circ}C$ but survived short at $37^{\circ}C$ or above.

• Korean Journal of Veterinary Research
• /
• v.33 no.3
• /
• pp.429-442
• /
• 1993

The cytotoxic effects of S hyodysenteriae strain B 204 on the mucosal surface were studied in surgically prepared ligated colonic loops in two male convenitonal mixed-breed pigs. In each one of four loops was inoculated with either sterile trypticase soy broth(TSB) of Serpulina, filtrate of Serpulina TSB culture. washed Serpulina cells or whole culture of Serpulina. Mucosal tissues were examined by transmission and scanning electron microscopy 24 and 48 hours after inoculation(p.i.). The filtrate did not induce any significant effect on the mucosal surface. The washed cells produced early lesions similar to those caused by the whole culture. These observations suggest that cytotoxins of the culture do not play a significant role in invasion of the epithelium in this experimental infection. The possible role of toxins associated with the organism at the site of interaction with the epithelial cells was not elucidated.

• Korean Journal of Veterinary Research
• /
• v.40 no.1
• /
• pp.72-80
• /
• 2000

Lipopolysaccharides (LPS) of Pasteurella multocida (P multocida) and several Gram-negative bacterial pathogens were analyzed by methanolysis, trifluoroacetylation and gas chromatography (GC) on a fused-silica capillary column. The GC analysis indicated that LPS prepared from a strain of P multocida by phenol-water (PW) or trichloroacetic acid (TCA) extraction were quite different in chemical composition. However, LPS prepared from Salmonella enteritidis by the two extraction methods were very similar. PW-LPS extracts from different Pasteurella strains of a serotype had essentially identical GC patterns. Endotoxic LPS extracted from 16 different serotypes of P multocida by PW or by phenol-chloroform-petroleum ether procedures yielded chromatograms indicating similar composition of the fatty acid moieties but minor differences in carbohydrate content. When the chemical composition of endotoxic LPS extracted from several Gram-negative bacteria (P multocida, Pasteurella hacmolytica, Haemophilus somnus, Actinobacillus ligniersii, Brucella abortus, Treponema hyodysenteriae, Escherichia coli, Bacteriodes fragilis, Salmonella abortus equi and Salmonella enteritidis) were examined, each bacteria showed a unique GC pattern. The carbohydrate constituents in LPS of various Gram-negative bacteria were quite variable not only in the O-specific polysaccharides but also in the core polysaccharides. The LPS of closely related bacteria shared more fatty acid constituents with each other than with unrelated bacteria.