• Korean Journal of Food Science and Technology
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• v.32 no.4
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• pp.970-973
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• 2000

Heavy metal contents and parasite egg examination for the safety evaluation of dropwort(Oenanthe javanica) from the inside of Chonnam province(5 regions) and suburbs of Kwang-ju(1 region) were investigated. The contents of heavy metals(Hg, Pb, As, Cd, Mn, Zn, Cu) were determined by the Atomic Absorption Spectrophotometer and parasite eggs were examined to various internalparasites(F. hepatica, C. sinesis, G. lamblia, Cryptosporidium, A. lumbricoides and A. duodenale). First, Hg and As were detected in trace level and $0.003{\sim}0.01\;ppm$. Pb was not detected in all samples and Cd contents of dropworts were detected $0.014{\sim}0.087\;ppm$. The contents of 4 kinds of heavy metals(Hg, As, Pb and Cd) in dropworts cultivated in various regions(6 regions) were not detected or trace levels. Therefore, dropworts cultivated in Chonnam province and suburbs of Kwang-ju were free from contamination of heavy metals. Moreover, the result of parasite egg examination(Trematoda, Protozoa and Nematoda) was not detected in all samples.

• Parasites, Hosts and Diseases
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• v.41 no.3
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• pp.155-163
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• 2003

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.173-180
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• 1991

A SEM study was performed on the surface of adult P. habayashii Park, 1940, recovered from the snake, Elaphe rufodorsata. The anterior part of the worms was cup-shape and equipped with oral, ventral suckers, pseudosuckers, and tribocytic organ, and the posterior one was finger-like and round-ended, The tegument of the anterior body was covered with 3~4 pointed small spines on the midventral surface and 1~2 pointed ones on the lateral surface. Sensory papillae such as type II, dome-shape ones, and papillae with an opening were distributed over the ventral surface of the anterior portion. The round tribocytic organ was bearing small stout spines laterally, whereas the surface Which comes in contact with the host tissues consisted of numerous long fibrillar fibers. The lip of the oral sucker contained type II papillae. Lateral margin of the anterior body revealed type III papillae.

• Parasites, Hosts and Diseases
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• v.55 no.5
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• pp.575-578
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• 2017

Aspidogastrid trematodes (Subclass Aspidogastrea) are a relatively small group with a characteristic adhesive disc and parasitize in a variety of cold-blooded hosts. Until now, only 2 species in the genus Cotylaspis, i.e., C. coreensis and C. sinensis, have been reported as the aspidogastrid trematode in the Republic of Korea (=Korea). In the present study, we intended to describe a species of aspidogastrids collected from the small intestine of the common carp, Cyprinus carpio, in a faunistic point of view. Total 51 specimens were collected from a carp caught in Nakdong-gang (River) on May 2015. Some of them were prepared as the specimens for light microscopic observations, and some others were prepared for SEM. They were slightly elongated without head lobes, $2,432{\times}840{\mu}m$ in average size, and had characteristic adhesive discs with 4 rows and 46 alveoli in average. The ovary was reniform and was located in the posterior-upper part of the body. The single testis was larger than the ovary and was located below the ovary. The uterus was coiled containing numerous eggs and distributed in the posterior 2/3 of the body. The vitellaria were follicular, and distributed from the mid-level of testis to near the posterior end. The morphological characters with dimensions of our specimens were closely identical with those of Aspidogaster ijimai previously described. A new aspidogastrid is added among the Korean trematode fauna by the present study.

• Parasites, Hosts and Diseases
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• v.52 no.3
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• pp.287-290
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• 2014

Human cases of echinostomiasis have been sporadically diagnosed by extracting worms in the endoscopy in Korea and Japan. Most of these were caused by Echinostoma hortense infection. However, in the present study, we detected 2 live worms of Echinostoma cinetorchis in the ascending colon of a Korean man (68-year old) admitted to the Gyeongsang National University Hospital with complaint of intermittent right lower quadrant abdominal pain for 5 days. Under colonoscopy, 1 worm was found attached on the edematous and hyperemic mucosal surface of the proximal ascending colon and the other was detected on the mid-ascending colon. Both worms were removed from the mucosal surface with a grasping forceps, and morphologically identified as E. cinetorchis by the characteristic head crown with total 37 collar spines including 5 end-group ones on both sides, disappearance of testes, and eggs of $108{\times}60{\mu}m$ with abopercular wrinkles. The infection source of this case seems to be the raw frogs eaten 2 months ago. This is the first case of endoscopy-diagnosed E. cinetorchis infection in Korea.

• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.214-220
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• 1985

Four cases of human infection by Echinochasmus japonicus (Trematoda; Echinostomatidae) were proven by the recovery of adult worms after a treatment with praziquantel (10mg/kg in single dose) and purgation in February 1984 in Korea. The patients were 3 middle-aged men and 1 elderly woman residing in a riverside village of Kangjin-gun (Tamjin river), Jeonranam-do, an ever-known endemic area of metagonimiasis. The treatment revealed numerous M. yokogawai worms together with 1-43 (total 54) specimens of E. japonicus and/or a few number of other kinds of trematodes or cestodes. The patients had gastrointestinal troubles such as indigestion and abdominal discomfort, probably due to these fluke infections. They have been eating some raw flesh of fresh water fishes such as cyprinoid ones or sweet fish caught from the river. This is the first record of human E. japonicus infection in the literature.

• Parasites, Hosts and Diseases
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• v.53 no.2
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• pp.201-207
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• 2015

This study aimed to investigate the infection status, worm development, and phylogenetic characteristics of the intestinal trematode, Stellantchasmus falcatus. The metacercariae of S. falcatus were detected only in the half-beak (Dermogenus pusillus) out of the 4 fish species examined. Their prevalence was 90.0%, and the intensity of infection was 919 metacercariae on average. Worms were recovered from 33 (97.1%) of 34 chicks that were experimentally infected with 200 S. falcatus metacercariae each, and the average recovery rate was 43.0%. The body size and inner organs of S. falcatus quickly increased in the experimental chicks over days 1-2 post-infection (PI). In addition, ITS2 sequence data of this parasite were analyzed to examine the phylogenetic relationships with other trematodes using the UPGMA method. The results indicated that the ITS2 sequence data recorded from trematodes in the family Heterophyidae appeared to be monophyletic. This study concluded that D. pusillus serves as a compatible second intermediate host of S. falcatus in Thailand and that S. falcatus can develop rapidly in the experimental chicks. Data collected from this study can help to close the gap in knowledge regarding the epidemiology, biology, and phylogenetic characteristics of S. falcatus in Thailand.

• Parasites, Hosts and Diseases
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• v.27 no.4
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• pp.231-248
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• 1989

The growth and development of the metacercariae of F. seoulensis cultivated in vitro or on the chick chorioallantois were assessed by comparison with the optimum process of maturation in albino rats and new born chickens. The process of maturation was divided for convenience into six stages: Stage 1 ; cell multiplication, Stage 2; body shaping, Stage 3; separation of genital anlagen, Stage :1 organogeny, Stage 5; gametogony, and Stage 6: oviposition. In Hank's and Tyrode's .solutions, the metacercariae were alive up to 200 days or more at $4^{\circ}C$ without any development. The in vivo maturation process in rats or chicks was as follows: stage 1 from 6 hours; stage 2 from 24 hours; stage 3 from 48 to 72 hours; stage 4 from 3 to 4 days; stage 5 from 4 to 5 days; and stage 6 from 5 to 8 days. Despite unsuccessful infection of the metacercariae to 12 day old chicks, fully mature worms of stage 5 or 6 were recovered from new born chicks (1 to 2 days old), The metacercariae of F. seoulensis grown in vitro were up to stage 3 and no further maturation was observed. Of various media employed, the medium NCTC 109 (Gibco) or NCTC 135(Gibco) supplemented with 20% egg yolk or 20% whole egg macerate or 0.5% yeast was basically required for the earlier development of the fluke. It took 16.1 days(in average) to reach the stage 3 after cultivation. The metacercariae cultivated on the chorioallantoic membranes of 6∼13 day old chick embryo at 37∼38℃ showed their full development up to stage 5 or 6. However, the worms were in general remarkably retarded, compared with those grown in rats or chickens. In the experiments of worm transplant, although the transfer was failed from in vitro culture to in vivo of rats(Per os), the transplants from in vitro culture to the chorioallantois and from the choriollantois to in vivo of rat host were successful with or without development of the transferred worms. In the present study, it was observed that the metacercariae of F, seoulensis can be maintained in vitro media with poor development as well as fully matured in 1 to 2 day-old chicks or on the chorioallantois at a very low rate.

• Parasites, Hosts and Diseases
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• v.27 no.4
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• pp.253-260
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• 1989

Metacercariae of the genus Stictodora encysted in the head tissue of Acanthogobius navimanus (the gobies) caught at Sachun-gun, Kyongnam Province, were identified to be Stictodora Zari Yamaguti, 1939 (Trematoda: Heterophyidae), a new parasite fauna in Korea. The metacercariae were 0.39∼,0.43 mm by 0.32∼0.35 mm in size, long elliptical, and with a thin and transparent cyst wall. Total 200 metacercariae were collected from 50 gobies. In order to obtain adult worms two kittens and a Puppy were infected each with 34∼100 metacercariae, and total 33 adults were recovered between the day 4 and day 8 post-infection. The S. sari adults measured 0.95∼1.18 mm long and 0.26∼0.32 mm wide and the eggs in uteri 0.028∼0.033 mm by 0.017∼0.020 mm. The most characteristic morphological feature of these flukes was the presence of a gonotyl and gonotyl spines arranged in two groups; densely crowded group of 30~40 spines and linearly-arranged one of 30∼40 spines, together of which made a comma(or reversed comma) shape along the lateral margin of the gonotyl. It has been proved by this study that 5. sari is distributed in southern coasts of Korea.

• Journal of Veterinary Clinics
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• v.33 no.3
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• pp.151-154
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• 2016

The trematodes of Genus Nasitrema are the most common infection in the heads of small odontocetes. Five species of Nasitrema have been described in Asia. But those parasites has been not surveyed in the East Sea of Korean peninsula. There are no evidence of pathology associated with Nasitrema attenuate. To determine the infection ratio of N. attenuata in the dolphin, it was surveyed 9 long-beaked common dolphin, Delphinus capensis, caught incidentally from Samcheok to Pohang, East Sea, Korea. Five males and four females, estimated to be between 1 to 10 years old, were examined. The quantity of N. attenuata harvested by aspiration from pterygoid sinuses ranged from 10 to 100 with a 100% (9/9) infection with older dolphins exhibiting higher parasite counts. The body of trematoda was elongated with tegumental patches resembling the leaves of lilies and oral sucker located ventrally at the anterior terminal. Esophagus was absent and intestinal bifurcation arose immediately from the posterior of pharynx. The undulated intestinal caeca transversed the entire body and terminated at the posterior extremity of the worm. The genital pore opened anterior to the ventral sucker. This report confirms the prevalence and morphology of N. attenuata in the dolphins from the East Sea, Korea.