• Journal of the Korean Institute of Gas
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• v.18 no.4
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• pp.86-94
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• 2014

To develop a Root Cause Analysis Map which determines the cause of the accident in chemical laboratory, The Root Cause Analysis(RCA) Map for the laboratory areas was sketched from Phase 1 of the accident element to Phase 3 of the accident element, based on the RCA Map which is applied in the petrochemical industry. On the basis of laboratory RCA Map which was classified by using such method. The root causes of the 211 accident cases in laboratories were classified from Phase 4 to Phase 5 by the Cause Factor Charting technique and The cause of the accident data were inputted to EXCEL program. After that, The causes of the accident data were sorted and classified by type and each step. So 'Approximate Primary RCA Map Draft' was written. In addition, it was reaffirmed whether the root causes of 211 accidents of laboratory were appropriate to 'Primary RCA Map Draft'. By complementing the cause which was expected to cause future accidents, the RCA Map for chemical laboratories was developed. Based on 'RCA Map' proposed in this study, the causes of accidents were analysed management systems 35%, monitoring 12.2%, Human Factor Eng. 15.1% and education training 12.1% by the size of the frequency from Phase 1 to Phase 5.

• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.15-29
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• 1995

To investigate plant community structure of Uiryung valley, Harujae valley, Kugi valley, Songchu valley, Tobongsan valley in Bukhansan National Park, each thirty eight, forty, twenty six. twenty eight, thirty six plots (plot size 100$m^2$) were set up and surveyed. The surveyed data were integrated and classification by TWINSPAN and DCA ordination techniques were applied to the study area. The plant community were divided into nine groups in seventy nine plots by TWINBPAN and the dividing groups were Carpinus laxiflora community(I), Quercus mongolica community(II), Pinus densiflora community(III), Q. mongolica-P. densiflora community(IV) Q. mongolica-Q. serrata- Prunus sargentii community(V), Q. mongolica Q. variabilis community(Ⅵ), Robinia pseudoacacia-Q. serrata community(Ⅶ). P. rigida-Q. serrata community(Ⅷ), Q. mongolica- Q. variabilis- Styrax obassia community(Ⅸ). So the successional trends of tree species at the natural forest seem to be from P. densiflora to Q. mongolica and at the artificial forest seem to be from R. pseudoacacia, P. rigida to Q. acutissima, Fraxinus rhynchophylla in canopy layer And in the subtree and shrub layer, it wag supposed that from Deotzia prunifolia, Rhus trichocarpa. Rhododendron yedoense var. poukhasleilse, Euonymus oxyphyllous to Magnolia sieboldii, Acer psuedo-sieboldianum, Lindera obtusiloba. In comparing successional trends with past study, it was postulated that successtion is not progressed by human disturbance and soil acidification by the air pollution.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.5
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• pp.394-404
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• 2007

Oral squamous cell carcinoma is the most prevalent oral cancer, which is characterized by its high metastasis and recurrent rates and poor prognosis. Taxol is an anticancer agent which is microbial products extracted from jew tree. It combines with the tubulin and induces apoptosis by inhibiting mitosis of cell with microtubule stabilization. Recently, it was reported to be effective in various solid tumors, but only very slight effect has been seen in oral squamous cell carcinomas due to its cell-specific potencies. Cyclosporin A is used as immune suppressant and is being applied in anticancer therapy as its mechanism of induction of change of apoptotic process in various cells have been known. In this study, oral squamous cell carcinoma HN22 cell line was used for in vitro experiment and as for the experimental group taxol and cyclosporin A were applied alone and to observe the synergistic effect of apoptosis, Taxol and cyclosporin A were coadministered with different concentration of taxol for comparison. The results were obtained as follow: 1. There was no difference in Bcl-2, Bax, caspase 3, 8, 9 mRNA expression when cyclosprin A or taxol was applied alone to HN 22 cell line. 2. Caspase 3, 9 mRNA expression was prominently increased when cyclosprin A and taxol were applied together to cancer cell. 3. No significant difference was observed when cyclosporin A and taxol($1{\mu}g/ml$ and $3{\mu}g/ml$) were applied together to cancer cell line. 4. No significant difference was seen in Bcl-2, Bax, and caspase 8 mRNA expression in all the groups of in vitro experiments. 5. When cyclosporin A was applied alone in vivo study on the nude mice, histopathologi cal findings was similar to those of the control group. Oral squamous cell carcinoma induced by inoculation of HN 22 cell line was not reduced after treatment of cyclosporin A. 6. When taxol was applied alone, the islands of squamous cell carcinoma still remained, which meant insignificant healing effect. There was a lesser volume increase compared with the cyclosporin A alone. 7. When taxol and cyclosporin A were applied together, the connective tissue and calcification were seen in the histopathologic findings. Oral squamous cell carcinoma was decreased and cancer cell was disappeared. In observing the tumor mass change with time, there was a gradual decreased size and healing features. As the results of the in vitro experiment, it could conclud that only when the two agents are applied together, mitochondria-mediated apoptosis occurred by considerable increase of caspase 3, 9 mRNA expression, irrespectable of the concentration of taxol. In vivo experiment, there was a discrete synergistic effect when the two agents were applied together. But single use of cyclosporin A was not effective in this study. Based on the results of this experiment, if further clinical studies are done, taxol and cyclosporin A could be effectively used in treatment of oral squamous cell carcinomas.

• Journal of Life Science
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• v.24 no.6
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• pp.612-617
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• 2014

The phenetic relationships among six natural populations of Equisetum pratense in Korea were investigated at the population level by constructing a tree based on Random Amplified Polymorphic DNA (RAPD) markers. RAPD analysis was also conducted to estimate genetic diversity and the population structure of E. pratense. A mean of 26.7% at the six population levels indicated polymorphism. E. pratense was found to have fewer alleles per locus (1.267) and fewer effective alleles per locus (1.176). Genetic diversity (0.102) in E. pratense is lower than the average for species with similar life history traits. Total genetic diversity values (HT) varied between 0.112 (OPD-07) and 0.445 (OPD-16), for an average overall polymorphic locus of 0.141. Inter-locus variation in the within-population genetic diversity ($H_S$) was low (0.102). Asexual reproduction, small population size, and the colonization process are proposed as possible factors contributing to the observed low genetic diversity in E. pratense. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.129 for OPD-07 to 0.455 for OPD-09, with a mean of 0.277. This indicated that about 27.7% of the total variation was among populations. Thus, genetic variation (72.3%) resided within populations. This study contributes new information for research on the taxonomy and population genetics of E. pratense.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.553-558
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• 2010

This study was conducted to clarify the differences in rodents population densities between natural restored and red pine silvicultured forests after forest fire in Samcheok, Gangwon Province, Korea from March to December 2008. One ha size of 3 study plots were set up in each natural restored and silvicultured stand. We trapped the small rodents during 4 consecutive nights every 2 months in each stand. Understory coverage and number of shrub stems were higher in silvicultured stand than in natural restored stand. Coverage of overstory, suboverstory and midstory, number of tree stem, woody seedling stems and dead wood, and amount of coarse woody debris were higher in natural restored stand than in silvicultured stand. Six hundred eighty eight individuals of four species, such as Apodemus agrarius, A. peninsulae, Eothenomys regulus and Tamias sibiricus were captured in our study. Number of captured small rodents were higher in natural restored stand than in silvicultured stand. Also, species compositions were differed in both stands. The captured number of A. agrarius and A. peninsulae were most highest in April and December. E. regulus were shown higher number of captured in April and June, and T. sibiricus were in June and October. Removal of coarse woody debris and silvicultural practice would not be good for the inhabitation of small rodents. For the conservation of small rodents diversity, management of understory and canopy would be needed in forest fired area.

• The KIPS Transactions:PartD
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• v.18D no.4
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• pp.225-236
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• 2011

Various applications of LBS (Location Based Services) are being developed to provide the customized service depending on user's location with progress of wireless communication technology and miniaturization of personalized device. To effectively process an amount of vehicles' location data, LBS requires the techniques such as vehicle observation, data communication, data insertion and search, and user query processing. In this paper, we propose the historical location index, GIP-FB (Group Insertion tree with Flexible Buffer Node) and the flexible buffer node technique to adjust the cost of data insertion and search. the designed GIP+ based index employs the buffer node and the projection storage to cut the cost of insertion and search. Besides, it adjusts the cost of insertion and search by changing the number of line segments of the buffer node with user defined time interval. In the experiment, the buffer node size influences the performance of GIP-FB by changing the number of non-leaf node of the index. the proposed flexible buffer node is used to adjust the performance of the historical location index depending on the applications of LBS.

• Journal of KIISE:Databases
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• v.33 no.6
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• pp.620-630
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• 2006

Event-detection is an important application of sensor networks. Join operations can facilitate event-detection with a condition table predefined by a user. When join operations are used for event-detection, it is desirable, if possible, to do in-network join processing to reduce communication costs. In this paper, we propose an energy-efficient in-network join algorithm, called PBA. In PBA, each partition of a condition table is stored along the path from each node to the base station, and then in-network joins are performed on the path. Since each node can identify the parts to store in its storage by its level, PBA reduces the cost of disseminating a condition table considerably Moreover, while the existing method does not work well when the ratio of the size of the condition table to the density of the network is a little bit large, our proposed method PBA does not have such a restriction and works efficiently in most cases. The results of experiments show that PBA is efficient usually and especially provides significant cost reduction over existing one when a condition table is relatively large in comparison with the density of the network, or the routing tree of the network is high.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.262-268
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• 2014

A fungal isolate DUCC5000 from a garden plant Pachysandra terminalis was identified as Paraconiothyrium brasiliense based on the results of morphological and molecular studies. The fungus formed brown to black conidiomata of (0.2-0.7)-2(-3.5) mm singly or as a group on PDA. Conidia measured $2-5{\times}1.8-3{\mu}m$ in size, hyaline, ellipsoid to short-cylindrical, and rounded at both ends. The internal transcribed spacer (ITS) DNA of the isolate shared 100% nucleotide sequence homology with those of known P. brasiliense isolates. Phylogenetic tree inferred from the ITS sequence analysis showed that the DUCC5000 isolate formed a clade with known isolates of P. brasiliense. The fungal mycelia grew better on oatmeal agar than on MEA and PDA. On PDA media under various pH conditions, fungal mycelial growth was observed at pH 9. Colony morphology of the fungus tended to alter depending on the kinds of nutrient media and pH condition. On chromagenic media, the fungus demonstrated its ability to produce extracellular enzymes including amyalse, avicelase, ${\beta}$-glucosidase, protease, and xylanase. However, in pathogenicity testing, no disease symptoms were observed on the leaves of P. terminalis. This strain is the first report on P. terminalis in Korea.

• Korean Journal of Medicinal Crop Science
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• v.18 no.3
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• pp.143-150
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• 2010

This study showed the increase of antitumor activities of water soluble E. sinica extract by nano-encapsulation process with lecithin. Five groups of lecithin only group (LO), lecithin nano-encapsulated E. sinica group (LE), E. sinica only group (EO), one negative control group (NCO) and positive control group (PCO) were set for several anticancer experiment and fed into Sarcoma-180 injected mice. The cytotoxicity of LE on the human normal kidney cell (HEK293) showed 14.8% lower than 19.2% of EO and 18.4% of LO. Growth of human liver carcinoma cell and human stomach carcinoma cell as representative of digestive system in vitro was inhibited up to about 85.1% and 87.3%, in adding 1.0 mg/$m{\ell}$ of LE, which values 15% higher than that from conventional EO. The survival rates of each mice group were 40%, 63%, 48%, 33% and 100%, respectively after 40 days of injecting Sarcoma-180. The increment of their body weights of the extract feeding groups was suppressed down to 10~15%, compared to the negative control. The nano-particles also reduced the hypertrophy of the internal organs such as spleen and liver down to 15~20%, compared to those as the other groups. Among them, LE effectively reduced the size of tumor form to 20%. From these results, in vitro and in vivo antitumor activities of E. sinica could be enhanced by using nano-encapsulation process with lecithin because of better permeation into the cancer cells by confocal observations.

• Korean Journal of Ichthyology
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• v.33 no.3
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• pp.157-166
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• 2021

Three specimens, presumed to be natural hybrids between Rhodeus notatus and R. ocellatus, were collected from Onyangcheon Stream area, Jwabu-dong, Asan-si, Chungcheongnamdo, the Republic of Korea, and morphological and phylogenetic analyses were performed to clearly identify their parent species. The body color of the three natural hybrids was light greenish-brown on dorsal side, and the size of red area on the upper front of the dorsal fin and the outer margin of the anal fin generally showed intermediate characters between the parent species, R. notatus and R. ocellatus. Among the measurement and meristic characters, the ratio of prepectoral length and preanal length in the standard length, and the ratio of snout length, interorbital width, length of caudal peduncle and depth of caudal peduncle in the head length, and the number of longitudinal row scales were analyzed as the unique characters of natural hybrids. In the rag1 gene of nuclear DNA, the three natural hybrids were analyzed to be reflected all the single nucleotide polymorphism sites between R. notatus and R. ocellatus, and in the phylogenetic tree using the cytb gene of mitochondrial DNA, they formed the same genetic clade as R. notatus. Therefore, three specimens, presumed to be natural hybrids analyzed in this study were identified as interspecific hybrids between female R. notatus and male R. ocellatus.