• Title/Summary/Keyword: Tree Propagation

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In vitro introduction adventitious shoots and plant regeneration of sengon (Paraserianthes falcataria (L.) Nielsen) (셍온(Paraserianthes falcataria (L.) Nielsen)의 기내 부정줄기 유도 및 식물체 재분화)

  • Kim, Ji Ah;Moon, Heung Kyu;Kim, Yong Wook;Bae, Eun Kyung
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.235-238
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    • 2015
  • Adventitious buds were obtained from isolated cotyledons cultured on MS medium with various concentrations of 6-benzylamino purine (BA) and thidiazuron (TDZ). The highest numbers of adventitious buds were obtained on MS medium supplemented with 0.2 mg/L BA. Experimental culturing with half the petiole portion and half with the terminal segments were grown on MS medium contained with 0.2 mg/L BA. Frequency of the adventitious bud induction was variable accordingly to the type of cultured explants. Explants with the half petiole showed the highest adventitious bud induction rate (80%) compared to explants of half with terminal segment (20%). An elongated shoot from the buds and growth of advent roots were both possible on the 1/2 MS medium without a plant growth regulator. These results offer an effective way in which clonal propagation can be accomplished.

Micropropagation of Mature Betula davurica by Bud Cultures (물박달나무 (Betula davurica) 성숙목의 아배양에 의한 기내번식)

  • 문지연;문흥규
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.271-274
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    • 1999
  • This study was undertaken to develop an efficient propagation technique for mature Betula davurica. Using aseptic materials taken from in vitro culture, the effects of media and plant growth regulators on shoot proliferation and rooting were investigated. DKW medium turned out to be the best in shoot proliferation among the media tested. Whereas axillary buds were better culture material than apical buds in proliferation of shoots, apical buds were slightly better than axillary buds on shoot elongation. Neither 1 /2 MS nor WPM medium seemed to be suitable for shoot multiplication or elongation. When the explants were cultured on 1/2 MS medium, shoot elongation was retarded by forming big callus at the base. In the case of WPM, shoots could be formed normally, but they exhibited slow growing. NAA was so effective on in vitro rooting that more than 80% rooting could be achieved on half-strength DKW medium supplemented with 1.0 mg/L NAA after 4 weeks in cultures. Ex vitro rooting using elongated shoot was also applicable to rooting and acclimatization. Rooted plantlets were successfully acclimatized in an artificial soil mixture and grew normally. The results demonstrate that efficient mass propagation of mature B. davurica can be done through tissue culture.

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Production of Plug Plantlets for Mass Propagation Using Stem Cuttings of Virus Free Microtubers in Potato (감자 바이러스 무균종묘의 대량생산과 플러그화에 관한 기초 연구)

  • 박양문;소인섭;유장걸;강봉균
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.678-686
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    • 1997
  • This experiment was carried out to develop the mass propagation system for producing plug plantlets using stem cuttings of virus-tree microtubers in potato. Cocopeat, vermiculite, perlite and peatmoss were combined and used as plug nursery media to find out the best combination suitable for the growth of seedlings derived from microtubers. Seedling growth was favored in high temperature (above 2$0^{\circ}C$) and a long-day photoperiod(above 16 hours) condition, while stolons and microtubers formed in outdoor condition. Shoot and root multiplication was not affected by NAA 10mg /1 or IAA 10mg /1 treatment. At the early growth stage of plug plantlets, the number of leaves and roots and the length of root increased significantly when nodes from the upper (near to apex) part of shoots rather than from basal part were taken. But after transplanting, these differences among these characters were not observed. At ninety days after transplanting the plug plantlets in spring time, plant was around 70 to 80cm in height, and the number of stolons and tubers were ten and seven, respectively.

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Application of Probabilistic Tsunami Hazard Analysis for the Nuclear Power Plant Site (원자력 발전소 부지에 대한 확률론적 지진해일 재해도 분석의 적용)

  • Rhee, Hyun-Me;Kim, Min Kyu;Sheen, Dong-Hoon;Choi, In-Kil
    • Journal of the Earthquake Engineering Society of Korea
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    • v.19 no.6
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    • pp.265-271
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    • 2015
  • The tsunami hazard analysis is performed for testing the application of probabilistic tsunami hazard analysis to nuclear power plant sites in the Korean Peninsula. Tsunami hazard analysis is based on the seismic hazard analysis. Probabilistic method is adopted for considering the uncertainties caused by insufficient information of tsunamigenic fault sources. Logic tree approach is used. Uljin nuclear power plant (NPP) site is selected for this study. The tsunamigenic fault sources in the western part of Japan (East Sea) are used for this study because those are well known fault sources in the East Sea and had several records of tsunami hazards. We have performed numerical simulations of tsunami propagation for those fault sources in the previous study. Therefore we use the wave parameters obtained from the previous study. We follow the method of probabilistic tsunami hazard analysis (PTHA) suggested by the atomic energy society of Japan (AESJ). Annual exceedance probabilities for wave height level are calculated for the site by using the information about the recurrence interval, the magnitude range, the wave parameters, the truncation of lognormal distribution of wave height, and the deviation based on the difference between simulation and record. Effects of each parameters on tsunami hazard are tested by the sensitivity analysis, which shows that the recurrence interval and the deviation dominantly affects the annual exceedance probability and the wave heigh level, respectively.

Germination Characteristics and Early Growth of Acer pictum subsp. mono (Maxim.) H. Ohashi by Harvesting Dates (고로쇠나무 종자 수확시기에 따른 발아특성 및 초기생육)

  • Song, Ki Seon;Yoon, Jun Hyuck;Jeon, Kwon Seok;Kim, Chang Hwan;Park, Yong Bae;Kim, Jong Jin
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.1
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    • pp.21-26
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    • 2016
  • Background : Acer pictum is a deciduous tree belonging to the family Aceraceae. This study was conducted to collect basic data on mass propagation techniques by surveying the germination and growth characteristics of A. pictum seeds. Methods and Results : A. pictum seeds were harvested in 2014 on August 19, September 5, September 29 and October 31. The seeds were then sown on the same dates they were harvested. A portion of seeds harvested in August 2014 were stored at low temperatures and subsequently sown on March 11, 2015. The germination rate of A. pictum seeds was highest in seeds that were stored harvested on October 31. Mean germination time (MGT) was shortest and germination velocity (Rs) was fastest in seeds harvested on October 31. Root collar diameter, total root length, and dry weight were also highest from seeds collected October 31. Conclusions : A. pictum seeds harvested on October 31 had highest germination and growth rates compared with seeds harvested on earlier dates. It is expected that these results demonstrating optimum harvesting and sowing dates will be applicable to future seedling production for this tree species.

Improvement of asymbiotic seed germination and seedling development of Cypripedium macranthos Sw. with organic additives

  • Huh, Yoon Sun;Lee, Joung Kwan;Nam, Sang Young;Paek, Kee Yoeup;Suh, Gang Uk
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.138-145
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    • 2016
  • To find the optimal propagation condition for endangered Cypripedium macranthos Sw., also known as lady's slipper orchid, the effect of various organic additives on in vitro germination, protocorm formation and seedling growth was investigated during asymbiotic seed culture. When $100ml{\cdot}L^{-1}$ coconut water was added to the basal medium, the highest germination rate and protocorm formation rate were achieved, with 70.8% and 74.2% respectively. Supplementation of phloem sap from birch tree or maple tree also showed a facilitating effect to improve the germination and protocorm development. With $100ml{\cdot}L^{-1}$ birch sap or maple sap, both the germination and protocorm formation rates were roughly more than 65% and 68%. The roots and buds of the seedlings grew vigorously in the medium containing $100ml{\cdot}L^{-1}$ coconut water or phloem sap, in particular, their bud formation rates increased by more than 70%. Addition of banana powder and peptone could not create a more significantly favorable culture condition, and non-addition had the worst results. Our results demonstrated that proper organic amendments such as coconut water and phloem sap might be preferred to in vitro germination and the growth of seedlings developed from the protocorm of C. macranthos Sw. during asymbiotic seed culture.

Effects of BA Treatment and Cut Planting in Root Cuttings of Lacquer Tree (Rhus verniciflua Stokes) (옻나무 근삽시 BA 처리방법과 삽식법의 효과)

  • 두홍수;권태호
    • Korean Journal of Plant Resources
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    • v.15 no.1
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    • pp.43-49
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    • 2002
  • Effects of BA treatment and cut planting methods on shooting and seedling characteristics were investigated to increase seedling products by cuttings of lacquer tree. Dipping on 1,000mg/L BA solution was better than dripping with same solution at 5 weeks after cut planting. As shooting rate was increased suddenly, it was 85% at 10 weeks after cut planting. Among 3 dripping treatments, interval was not effective on shooting rate. Dripping per 2 days with 100 ㎕ of 1,000 mg/L BA solution was most effective on seedling height and leaf number and than dipping was more effective than dripping per 5 and 7 days. Branch developed on near part from dripped scion. Shooting of horizontal planting was earlier about a week and rate was increased suddenly from 3 to 6 weeks, it was highest with 96% than others at 10 weeks after planting. Seedling of horizontal cut planting was tallest about 40cm and branch developed lower than vertical and oblique cut planting. In case of horizontal cut planting, 2-3 shoots were formed on different part of scion and than those were could divided to seedling, so horizontal cut planting was most effective to propagation of seedling than any others.

A Hierarchical Underwater Acoustic Sensor Network Architecture Utilizing AUVs' Optimal Trajectory Movements (수중 무인기의 최적 궤도 이동을 활용하는 계층적 수중 음향 센서 네트워크 구조)

  • Nguyen, Thi Tham;Yoon, Seokhoon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37C no.12
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    • pp.1328-1336
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    • 2012
  • Compared to terrestrial RF communications, underwater acoustic communications have several limitations such as limited bandwidth, high level of fading effects, and a large underwater propagation delay. In this paper, in order to tackle those limitations of underwater communications and to make it possible to form a large underwater monitoring systems, we propose a hierarchical underwater network architecture, which consists of underwater sensors, clusterheads, underwater/surface sink nodes, autonomous underwater vehicles (AUVs). In the proposed architecture, for the maximization of packet delivery ratio and the minimization of underwater sensor's energy consumption, a hybrid routing protocol is used. More specifically, cluster members use Tree based routing to transmit sensing data to clusterheads. AUVs on optimal trajectory movements collect the aggregated data from clusterhead and finally forward the data to the sink node. Also, in order to minimize the maximum travel distance of AUVs, an Integer Linear Programming based algorithm is employed. Performance analysis through simulations shows that the proposed architecture can achieve a higher data delivery ratio and lower energy consumption than existing routing schemes such as gradient based routing and geographical forwarding. Start after striking space key 2 times.

An Integrative Method of FTA and FMEA for Software Security Analysis of a Smart Phone (스마트 폰의 소프트웨어 보안성 분석을 위한 FTA와 FMEA의 통합적 방법)

  • Kim, Myong-Hee;Toyib, Wildan;Park, Man-Gon
    • KIPS Transactions on Computer and Communication Systems
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    • v.2 no.12
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    • pp.541-552
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    • 2013
  • Recently software security of the smart phone is an important issue in the field of information science and technology due to fast propagation of smart technology in our life. The smart phone as the security critical systems which are utilizing in terminal systems of the banking, ubiquitous home management, airline passengers screening, and so on are related to the risk of costs, risk of loss, risk of availability, and risk by usage. For the security issues, software hazard analysis of smart phone is the key approaching method by use of observed failures. In this paper, we propose an efficient integrative framework for software security analysis of the smart phone using Fault Tree Analysis (FTA) and Failure Mode Effect Analysis (FMEA) to gain a convergence security and reliability analysis technique on hand handle devices. And we discuss about that if a failure mode effect analysis performs simpler, not only for improving security but also reducing failure effects on this smart device, the proposed integrative framework is a key solution.

Optimization of factors influencing in vitro immature seed germination in Chionanthus retusus

  • Tar, Khin Yae Kyi;Naing, Aung Htay;Ai, Trinh Ngoc;Chung, Mi Young;Kim, Chang Kil
    • Journal of Plant Biotechnology
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    • v.45 no.4
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    • pp.347-356
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    • 2018
  • Chionanthus retusus is a small deciduous tree that is widely used in landscaping due to its beautiful white spring flowers and ornamental value. Conventional propagation through seeds requires one to two years of breaking dormancy. The objective of this study was to determine the conditions of in vitro germination in C. retusus. In vitro embryo culture was carried out to investigate the effects of six factors: basal media (McCown Woody Plant Medium (WPM) and Murashige and Skoog (MS)); plant growth regulators (different combinations and concentrations of naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BA), and gibberellic acid ($GA_3$)); embryo age (collected weekly beginning 36 days after fruit setting); low temperature pretreatment (storing $4^{\circ}C$ for 1, 2, 3, and 4 weeks); coconut additives (100, 200, and $300ml{\cdot}L^{-1}$); and genotype (grouping plants depending on their flowering nature). The basal medium used in this study was WPM with $2mg{\cdot}L^{-1-1}\;GA_3$, $20g{\cdot}L^{-1}$ sucrose, and $6g{\cdot}L^{-1}$ Agar. WPM medium mixed with $GA_3$, resulted in higher germination rate as compared to when using a combination of auxin and cytokinin. $GA_3$ at $2mg{\cdot}L^{-1}$ was the most effective of all combinations and concentrations of PGRs. WPM medium with $2mg{\cdot}L^{-1}GA_3$ resulted in better and faster germination (75.93%). Embryos collected at 57 days after fruit setting had the highest percent of germinated seeds (87.04%) while low-temperature pretreatment of fruits at $4^{\circ}C$ for two weeks produced the highest germination (95.37%). These results of this study could be an open ground for development of an efficient protocol for commercial production of the ornamental tree.