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http://dx.doi.org/10.5010/JPB.2015.42.3.235

In vitro introduction adventitious shoots and plant regeneration of sengon (Paraserianthes falcataria (L.) Nielsen)  

Kim, Ji Ah (Division of Forest Biotechnology, Korea Forest Research Institute)
Moon, Heung Kyu (Division of Forest Biotechnology, Korea Forest Research Institute)
Kim, Yong Wook (Division of Forest Biotechnology, Korea Forest Research Institute)
Bae, Eun Kyung (Division of Tree Improvement, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.42, no.3, 2015 , pp. 235-238 More about this Journal
Abstract
Adventitious buds were obtained from isolated cotyledons cultured on MS medium with various concentrations of 6-benzylamino purine (BA) and thidiazuron (TDZ). The highest numbers of adventitious buds were obtained on MS medium supplemented with 0.2 mg/L BA. Experimental culturing with half the petiole portion and half with the terminal segments were grown on MS medium contained with 0.2 mg/L BA. Frequency of the adventitious bud induction was variable accordingly to the type of cultured explants. Explants with the half petiole showed the highest adventitious bud induction rate (80%) compared to explants of half with terminal segment (20%). An elongated shoot from the buds and growth of advent roots were both possible on the 1/2 MS medium without a plant growth regulator. These results offer an effective way in which clonal propagation can be accomplished.
Keywords
Adventitious bud; Regeneration; Clonal propagation;
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