• Title/Summary/Keyword: Transport Mechanism

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Upregulation of Glutathion S-Transferase mu 1 in Bovine Cystic Follicles

  • Kang, Da-Won;Kim, Chang-Woon;Han, Jae-Hee
    • Journal of Embryo Transfer
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    • v.25 no.4
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    • pp.273-279
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    • 2010
  • Follicular cystic follicles (FCFs) show delayed regression with persistent follicle growth. However, the mechanism by which follicles are persistently grown remains unclear. Glutathione S-transferases (GSTs) are drug-metabolizing and detoxification enzymes that are involved in the intracellular transport and metabolism of steroid hormones. In this study, a proteomic analysis was performed to identify whether GST expression is changed in bovine FCFs and to predict the interactions between GST and other proteins. Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and 25 mm). In bovine follicles, GST mu 1 (GSTM1) was detected as a differentially expressed protein (DEP) and significantly up-regulated in FCFs compared to normal follicles (p<0.05). Consistent with the proteomic results, semi-quantitative PCR data and western blot analysis revealed an up-regulation of GSTM1 in FCFs. Expression levels of aromatase and dehydrogenase proteins were changed in FCFs. These results show that the up-regulation of GSTM1 that is observed in bovine FCFs is likely to be responsible for the persistent follicle growth in FCFs as the activity of aromatase and the dehydrogenases.

Signaling Molecules at the Conceptus-Uterine Interface during Early Pregnancy in Pigs

  • Seo, Heewon;Choi, Yohan;Shim, Jangsoo;Kim, Mingoo;Ka, Hakhyun
    • Journal of Embryo Transfer
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    • v.27 no.4
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    • pp.211-221
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    • 2012
  • The process of embryo implantation requires physical contact and physiological communication between the conceptus trophectoderm and the maternal uterine endometrium. During the peri-implantation period in pigs, the conceptus undergoes significant morphological changes and secretes estrogens, the signal for maternal recognition of pregnancy. Estrogens secreted from the conceptus act on uterine epithelia to redirect $PGF_2{\alpha}$, luteolysin, secretion from the uterine vasculature to the uterine lumen to prevent luteolysis as well as to induce expression of endometrial genes that support implantation and conceptus development. In addition, conceptuses secrete cytokines, interferons, growth factors, and proteases, and in response to these signals, the uterine endometrium produces hormones, protease inhibitors, growth factors, transport proteins, adhesion molecules, lipid molecules, and calcium regulatory molecules. Coordinated interactions of these factors derived from the conceptus and the uterus play important roles in the process of implantation in pigs. To better understand mechanism of implantation process in pigs, this review provides information on signaling molecules at the conceptus-uterine interface during early pregnancy, including recently reported data reported.

Charge Transport Characteristics of a-Se based X-ray Detector (비정질 셀레늄 기반의 X선 검출 센서의 전하 수송 특성)

  • Kang, Sang-Sik;Cha, Byung-Youl;Jang, Gi-Won;Kim, Jae-Hyung;Nam, Sang-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.11a
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    • pp.375-378
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    • 2002
  • There has recently been a great deal of interest in amorphous selenium for application of digital x-ray image sensor. The initial number of the electron-hole induced by interaction a-Se with x-ray photons and the collection efficiency to surface of generated charges are important parameters for x-ray sensitivity of the a-Se. Therefore, in this paper, we analyzed that thickness of a-Se film and electric field is affected on the initial number of electron-hole and the collection efficiency. The experimental value of x-ray induced charge about the various thickness and the electric field is compared with estimated absorbed energy through MCNP 4C code to analyze the mechanism x-ray induced signal of a-Se. The experimental results showed that the electric field depends on initial escape coefficient and the thickness depends on collection coefficient than escape efficient.

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Analysis of Submicron Gate GaAs MESFET's Characteristics Using Particle Model (입자모델을 이용한 서브마이크론 게이트 GaAs MESFET 특성의 해석)

  • 문승환;정학기;김봉렬
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.27 no.4
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    • pp.534-540
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    • 1990
  • In this paper the characteristics of submicron gate GaAs MESFET's have been studied using a particle model which takes into account the hot-electron transport phenomena, i.e., the velocity overshoot. \ulcornervalley(<000> direction), L valley (<111>direction), X valley (<100>direction) as the GaAs conduction energy band and optical phonon, acoustic phonon, equivalent intervalley, nonequivalent intervalley scattering as the scattering models, have been considered in this simulation. And the GaAs material and the device simulation have been done by determination of the free flight time, scattering mechanism and scattering angle according to Monte-Carlo algorithm which makes use of a particle model. As a result of the particle simulation, firstly the electron distribution, the potential energy distribution and the situation of electron displacement in 0.6 \ulcorner gate length device have been obtained. Secondly, the cutoff frequency, obtained by this method, is k47GHz which is in good agreement with the calculated result of theory. And the current-voltage characteristics curve which takes account of the buffer layer effect has been obtained. Lastly it has been verified that parasitic current at the buffer layer can be analyzed using channel depth modulation.

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Photoreactivation of the Oxygen Evolving Center in TIB-treated Chloroplasts of Spinach (TIB로 처리된 시금치의 엽록체에서 산소발생계의 광재활성화)

  • 정화숙
    • Journal of Plant Biology
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    • v.36 no.3
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    • pp.259-266
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    • 1993
  • In Tris-iso-butanol (TIB; Tris buffer pH 8.8 and 1% iso-butanol)-treated chloroplasts, oxygen evolving activity was more inhibited than Tris-treated chloroplasts, but restored highly by 2,6-dichlorophenol-indophenol (DCPIP) and photoreactivation. To understand the mechanism of this results of TIB in photosynthetic electron transport, system, oxygen consumption and evolution of PS I and PS II were measured and protein of the chloroplasts was analysed. In Tris- and TIB-treated chloroplasts, oxygen evolving activity was increased according to the light intensity. Under 48 W·m-2 light intensity, the oxygen evolving activity in both chloroplasts were similar but as the light intensity was increased, TIB-treated chloroplasts showed higher activity. Under 240 W·m-2 light intensity, TIB-treated chloroplasts showed about 25% higher oxygen evolving activity than Tris-treated chloroplasts. Oxygen evolving activity was increased after photoreactivation in both Tris-treated and TIB-treated chloroplasts. Addition of NH4Cl increased the activity in both chloroplasts but in TIB-treated chloroplasts the increase was 30% higher than that in Tris-treated chloroplasts. In PS I, oxygen evolving activity was not inhibited by both treatments whereas in PS II, significant difference was observed between two treatments. Addition of Mn2+ and Ca2+ enhanced oxygen evolution in both Tris- and TIB-treated chloroplasts. Though enhancement was higher in TIB-treated chloroplasts. No difference was observed n protein analysis of the two thylakoid membrane.

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Cake Reduction Mechanism in Coagulation-Crossflow Microfiltration Process (Crossflow 방식 응집-정밀여과 공정의 케이크층 저감 메커니즘)

  • Kim, Suhan;Park, Heekyung
    • Journal of Korean Society of Water and Wastewater
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    • v.17 no.4
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    • pp.519-527
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    • 2003
  • Cake layer in crossflow microfiltration(CFMF) can be reduced by coagulation, enhancing membrane flux. This is because enlarging particle size by coagulation increases shear-induced diffusivity and the back-transport of rejected particles. However it is known that the enlarged particles are disaggregated by the shear force of the pump while passing through it. This study is to look at the disaggregation in relation with cake layer reducation. Kaolin and polysulfon hollow fiber microfilter are used for experiment. The reduction of cake resistance by coagulation is observed in a range of 17% to 53% at the various coagulation conditions. The particle size analysis results of the experiments show that aggregated particles in feed are completely disaggregated by pump but re-aggregation of particles occurs in membrane. This suggestes that the re-aggregation of particles is critical to cake reduction and flux enhancement, since the aggregated particles are completely broken. The mechanisms for re-aggregation in membrane are the same with those for coagulation in feed tank. Charge neutralization is better for CCFMF than sweep flocculation although it has two drawbacks in operation.

The Study on the Recovery of Volatile Organic Components by Pervaporation (Pervaporation을 이용한 휘발성 유기성분 회수에 관한 연구)

  • 김희진;송영석;민병렬
    • Membrane Journal
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    • v.9 no.1
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    • pp.51-62
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    • 1999
  • The recovery of trace volatile organic components from water by pervaporation was investigated. Permeation experiments through homogeneous polydimethylsiloxane(PDMS) membrane was carried out and the effect of feed concentrations and membrane thicknesses on the permeation characteristics were investigated. A solution-diffusion model is used to describe the pervaporation transport mechanism. In homogeneous PDMS membrane it appeared that the selectivities of MEK and toluene are constant, and that organic flux has a linear relationship with feed concentration. These results indicate that the coupling effects between organics were negligible. The selectivity of PDMS membranes is invariant with respect to the membrane thickness. The intrinsic membrane permeability of organic components determined by using a solution-diffusion model. Comparing with various composite type membrane, the membrane using PEG treated nonwoven fabric as sublayer showed the best performance in VOC recovery by pervaporation.

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GIS Application Model for Spatial Simulation of Surface Runoff from a Small Watershed(I) (소유역 지표유출의 공간적 해석을 위한 지리정보시스템의 응용모형(I) -격자 물수지 모형의 개발 및 적용-)

  • 김대식;정하우
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.37 no.3_4
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    • pp.23-33
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    • 1995
  • Geographic data which are difficult to handle by the characteristics of spatial variation and variety turned into a possibility to analyze with tlie computer-aided digital map and the use of Geographic Information System(GIS). The purpose of this study is to develop and apply a GIS application model (GISCELWAB) for the spatial simulation of surface runoff from a small watershed. This paper discribes the modeling procedure and the applicability of the cell water balance model (CELWAB) which calculates the water balance of a cell and simulates surface runoff of watershed simultaneously by the interaction of cells. The cell water balance model was developed to simulate the temporal and spatial storage depth and surface runoff of a watershed. The CELWAB model was constituted by Inflow-Outflow Calculator (JOC) which was developed to connect cell-to-cell transport mechanism automatically in this study. The CELWAB model requests detail data for each component of a cell hydrologic process. In this study, therefore, BANWOL watershed which have available field data was selected, and sensitivity for several model parameters was analyzed. The simulated results of surface runoff agreed well with the observed data for the rising phase of hydrograph except the recession phase. Each mean of relative errors for peak discharge and peak time was 0.21% and2.1 1% respectively. In sensitivity analysis of CELWAB , antecedent soil moisture condition(AMC) affected most largely the model.

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Effects of Oxygen Supply and Mixed Sugar Concentration on ${\small{D}}$-Ribose Production by a Transketolase-Deficient Bacillus subtilis SPK1

  • Park, Yong-Cheol;Lee, Hae-Jin;Kim, Chang Sup;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.560-564
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    • 2013
  • ${\small{D}}$-Ribose is a value-added five-carbon sugar used for riboflavin production. To investigate the effects of oxygen supply and mixed sugar concentration on microbial production of ${\small{D}}$-ribose, a transketolase-deficient Bacillus subtilis SPK1 was cultured batch-wise using xylose and glucose. A change of agitation speed from 300 rpm to 600 rpm at 1 vvm of air supply increased both the xylose consumption rate and ${\small{D}}$-ribose production rate. Because the sum of the specific consumption rates for xylose and glucose was similar at all agitation speeds, metabolic preferences between xylose and glucose might depend on oxygen supply. Although B. subtilis SPK1 can take up xylose and glucose by the active transport mechanism, a high initial concentration of xylose and glucose was not beneficial for high ${\small{D}}$-ribose production.

Effective Streaming technology of a layered encoding Video Application supporting QoS mechanism in the Internet

  • Seok, Joo-Myoung;Lee, Kyou ho;Suh, Doug-Young
    • Proceedings of the IEEK Conference
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    • 2002.07c
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    • pp.2075-2078
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    • 2002
  • Internet became the most popular network in spite of its weakness in realtime multimedia service. Many experts believe that the Internet has the potential to become the main multimedia distribution network of the near future. Currently, it does not provide any (BoS guarantees and, even when it does, guaranteed quality delivery of video may turn out to be too expensive. Unavoidable packet losses and delay jitter caused by congestion in a best effort delivery environment require use of intelligent transport techniques for effective video delivery. According to market needs of better quality of service (QoS) fur realtime multimedia services over Internet, they have been standardizing RSVP, IntServ, and DiffServ This paper combines the benefits of QoS mechanisms such as RSVP/IntServ with scalable video encoding. We propose that more important bit stream is given more priority such that limited network resources are guaranteed far the stream. Various prioritizing approaches are proposed and compared to normal approach by using Network Simulator. The calculated QoS parameters such as packet loss rate are used to calculate degree of degradation in video quality. In this Paper, proposed methods can be implemented adaptively to Von protocol, such as H.323, SIP.

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