• Journal of Korean Medical Science
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• v.33 no.53
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• pp.298.1-298.10
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• 2018

Background: The renal function of individuals is one of the reasons for the variations in therapeutic response to various drugs. Patients with renal impairment are often exposed to drug toxicity, even with drugs that are usually eliminated by hepatic metabolism. Previous study has reported an increased plasma concentration of indoxyl sulfate and decreased plasma concentration of $4{\beta}$-hydroxy (OH)-cholesterol in stable kidney transplant recipients, implicating indoxyl sulfate as a cytochrome P450 (CYP) inhibiting factor. In this study, we aimed to evaluate the impact of renal impairment severity-dependent accumulation of indoxyl sulfate on hepatic CYP3A activity using metabolic markers. Methods: Sixty-six subjects were enrolled in this study; based on estimated glomerular filtration rate (eGFR), they were classified as having mild, moderate, or severe renal impairment. The plasma concentration of indoxyl sulfate was quantified using liquid chromatography-mass spectrometry (LC-MS). Urinary and plasma markers ($6{\beta}$-OH-cortisol/cortisol, $6{\beta}$-OH-cortisone/cortisone, $4{\beta}$-OH-cholesterol) for hepatic CYP3A activity were quantified using gas chromatography-mass spectrometry (GC-MS). The total plasma concentration of cholesterol was measured using the enzymatic colorimetric assay to calculate the $4{\beta}$-OH-cholesterol/cholesterol ratio. The correlation between variables was assessed using Pearson's correlation test. Results: There was a significant negative correlation between MDRD eGFR and indoxyl sulfate levels. The levels of urinary $6{\beta}$-OH-cortisol/cortisol and $6{\beta}$-OH-cortisone/cortisone as well as plasma $4{\beta}$-OH-cholesterol and $4{\beta}$-OH-cholesterol/cholesterol were not correlated with MDRD eGFR and the plasma concentration of indoxyl sulfate. Conclusion: Hepatic CYP3A activity may not be affected by renal impairment-induced accumulation of plasma indoxyl sulfate.

• Journal of agriculture & life science
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• v.45 no.6
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• pp.81-88
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• 2011

This study was carried out to provide basic information for standard cultivation guideline of Korean mountain cultivated ginseng (Panax ginseng). Environmental conditions, soil properties, growth process, cultivation methods and sowing timing were investigated in cultivations of Korean mountain cultivated ginseng in Korea (18 region in 9 province). Most of Korean mountain cultivated ginseng was cultivated to north, north-east, east or north-west direction in broad-leaved forest, IV age group, and 500~1,000 trees/ha. Elevation was below 500 m and slope was below $25^{\circ}$ in cultivation. There were significant differences in soil properties by region and cultivation based on organic matter (3.43~24.07%) and pH level (3.8~5.7). Sprouting, leaf unfolding, flowering, fruiting, red ripening, fruit drop and leaf drop of Korean mountain cultivated ginseng was investigated in cultivation. The results show that it took 98 days on average for the whole growth process. There were two methods, sowing and transplant for cultivation. Sowing methods were classified in sowing after opening-testa treatment and direct sowing. and it was 70% and 30%, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.3
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• pp.245-249
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• 1986

A pot and laboratory experiments were conducted to find out the effects of pretreatments of rice straw and silica fertilizer in submerged and upland soil condition on the evolution of nitrous oxide with different application time. The results obtained were summarized as follows; 1. Evolution of nitrous oxide was remarkably increased with pretreatment of rice straw and silica in upland condition than that of submerged condition. 2. Effects of application time of rice straw and silica fertilizer on the evolution of nitrous oxide were high in order of two weeks before transplant > early spring (February) > late autumn (November) application. 3. The consumption ratio of carbon for the evolution of one mole nitrogen was pronounced more in submerged condition than that of pretreated in upland condition. Application of rice straw with silica fertilizer was remarkably reduced the consumpotion of carbon on the denitrification path way. 4. Amount of mineral nitrogen as $NH_4^+-N$, $NO_2^--N$, and $NO_3^--N$, and nitrification rate were remarkably high in pretreatment of rice straw and silica in upland condition than that of contineusly submerged soil condition.

• Journal of Environmental Impact Assessment
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• v.30 no.6
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• pp.361-379
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• 2021

About 10% of trees damaged by the development projects are to be transplanted when conducting the Environmental Impact Assessment. However, various problems have been raised during transplantation. In this study, we confirm the stakeholder's perceptions of the problems that occur during transplantation. The survey was conducted from October 9 to 25, 2020. Among the stakeholder groups, 36 respondents participated in the consulting institute group, 44 from the review institute group, and 83 from the developer·agency group (total of 163). All three groups responded that it was necessary to transplant some of the damaged trees even if the development charge increased because the damage caused by the development project was serious. The most serious problem was 'high mortality'. The response rate was high that all three groups should plant the same species with the same quantity as an alternative method in case of withering. In order to reduce the mortality rate, small-sized trees were transplanted and transplanted trees were expanded to include planted species and landscape trees. In addition, the number of transplanted trees was high in response to calculating the transplantratio to the number of native tree damaged. The percentage of respondents who said that it was necessary to allocate a separate manager was also high. The results will be used as basic data to improve problems that occur during transplantation of damaged trees.

• Korean journal of dermatology
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• v.56 no.10
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• pp.603-608
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• 2018

Background: It is well known that skin cancer and precancerous disease develop more frequently in patients undergoing solid organ transplantation than normal populations in the normal population in Western countries. However, to date, the clinical and demographic features of skin cancer and precancerous disease after solid organ transplantation are not established in Asian countries. We evaluated the clinical and demographic features of primary skin cancer and precancerous lesions after solid organ transplantation and compared these with the trends observed in Western countries. Methods: We retrospectively reviewed the medical records of patients who underwent kidney, liver, heart, and lung transplantation between January 1995 and April 2017 and who developed skin cancer or precancerous lesions after transplantation. The various lesions observed were squamous and basal cell carcinoma, malignant melanoma, Kaposi sarcoma, Bowen's disease, and actinic keratosis. Results: We identified 4604 patients who received organ transplant. The mean age of patients was 44.8 years (male, 64.6%; female, 35.4%), and the sum of the person-year of observation time was 31,024 person-years. The incidence rate per 100,000 person-years was 29.01 for squamous cell carcinoma, 19.34 for basal cell carcinoma, 6.45 for malignant melanoma 3.22 for Kaposi sarcoma, and 74.17 for Bowen's disease and actinic keratosis. The incidence rate per 100,000 person-years was the highest in patients undergoing heart transplantation (610.50), followed by those who underwent kidney transplantation (136.54) and liver transplantation (90.15). Koreans showed lower incidence rates than those observed in Westerners. Conclusion: The incidence of primary skin cancer and precancerous lesions after solid organ transplantation in Koreans was lower than that in Westerners. Squamous cell carcinoma was the most common skin cancer in patients undergoing solid organ transplantation and the incidence rate of skin cancer and precancerous lesions was the highest in patients undergoing heart transplantation.

• Molecules and Cells
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• v.41 no.12
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• pp.1016-1023
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• 2018

Regenerative orthopedics needs significant devices to transplant human stem cells into damaged tissue and encourage automatic growth into replacements suitable for the human skeleton. Soft biomaterials have similarities in mechanical, structural and architectural properties to natural extracellular matrix (ECM), but often lack essential ECM molecules and signals. Here we engineer mineralized polysaccharide beads to transform MSCs into osteogenic cells and osteoid tissue for transplantation. Bone morphogenic proteins (BMP-2) and indispensable ECM proteins both directed differentiation inside alginate beads. Laminin and collagen IV basement membrane matrix proteins fixed and organized MSCs onto the alginate matrix, and BMP-2 drove differentiation, osteoid tissue self-assembly, and small-scale mineralization. Augmentation of alginate is necessary, and we showed that a few rationally selected small proteins from the basement membrane (BM) compartment of the ECM were sufficient to up-regulate cell expression of Runx-2 and osteocalcin for osteoid formation, resulting in Alizarin red-positive mineral nodules. More significantly, nested BMP-2 and BM beads added to a non-union skull defect, self-generated osteoid expressing osteopontin (OPN) and osteocalcin (OCN) in a chain along the defect, at only four weeks, establishing a framework for complete regeneration expected in 6 and 12 weeks. Alginate beads are beneficial surgical devices for transplanting therapeutic cells in programmed (by the ECM components and alginate-chitosan properties) reaction environments ideal for promoting bone tissue.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.285-291
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• 2018

The System of Rice Intensification (SRI) has been spread very quickly in southern Cambodia. To understand the motivation of farmers in adapting SRI, and its benefits, we conducted an on-farm study at Popel commune, Tramkak district, Takeo province in southern Cambodia, during the 2012 and 2013 wet season. We noticed a significant difference between SRI and conventional farmers' practices (FP) in rainfed lowland rice ecosystem. Despite of low nitrogen input, without chemical fertilizers, high grain yield was achieved in SRI 1 (6.0 t ha) and SRI2-Bottom ($7.2t\;ha^{-1}$) in 2013. SRI 1 and SRI 2 of panicle and number of panicle were high than SR 3, FP 2, and FP 3 due to early transplant. Relationships between total number of spikelet and plant nitrogen were ($r^2=0.95$) highly positive at harvest. SRI fields were, most of them achieving highly superior yield and number of panicle compared to their FP fields. The results indicated that SRI practices of planting younger seedling, with organic material and topography of paddy, lead to increased grain yield.

• Journal of Gastric Cancer
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• v.21 no.2
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• pp.191-202
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• 2021

Purpose: A near-infrared (NIR) fluorescence imaging is a promising tool for cancer-specific image guided surgery. Human epidermal receptor 2 (HER2) is one of the candidate markers for gastric cancer. In this study, we aimed to synthesize HER2-specific NIR fluorescence probes and evaluate their applicability in cancer-specific image-guided surgeries using an animal model. Materials and Methods: An NIR dye emitting light at 800 nm (IRDye800CW; Li-COR) was conjugated to trastuzumab and an HER2-specific affibody using a click mechanism. HER2 affinity was assessed using surface plasmon resonance. Gastric cancer cell lines (NCI-N87 and SNU-601) were subcutaneously implanted into female BALB/c nu (6-8 weeks old) mice. After intravenous injection of the probes, biodistribution and fluorescence signal intensity were measured using Lumina II (Perkin Elmer) and a laparoscopic NIR camera (InTheSmart). Results: Trastuzumab-IRDye800CW exhibited high affinity for HER2 (K_D=2.093(3) pM). Fluorescence signals in the liver and spleen were the highest at 24 hours post injection, while the signal in HER2-positive tumor cells increased until 72 hours, as assessed using the Lumina II system. The signal corresponding to the tumor was visually identified and clearly differentiated from the liver after 72 hours using a laparoscopic NIR camera. Affibody-IRDye800CW also exhibited high affinity for HER2 (K_D=4.71 nM); however, the signal was not identified in the tumor, probably owing to rapid renal clearance. Conclusions: Trastuzumab-IRDye800CW may be used as a potential NIR probe that can be injected 2-3 days before surgery to obtain high HER2-specific signal and contrast. Affibody-based NIR probes may require modifications to enhance mobilization to the tumor site.

• 한국사회정책
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• v.24 no.2
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• pp.181-215
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• 2017

The study found that the origins of modern Korean welfare regime are closely related to the political and economic order of the U.S. military rule between 1945 and 1948. The creation of developmental state in 1960s and 1970s can not be imagined from the standpoint of the U.S. military rule. The U.S. military government dismantled the labor movement and the farmers' movement, and dealt a devastating blow to leftist political forces. Through this process, the U.S. military government turned the political landscape of the Republic of Korea, which was dominated by left-wing political forces in August 1945, completely transformed into the political landscape dominated by right-wing political forces. Moreover, it would not have been possible without the physical force of the US military government to transplant American capitalism instead of the social (democratic) state that the majority of the Korean people wanted. Through farmland reform, the traditional landowning classes were broken down, the revolutionary farmers turned into conservative peasants, and the distribution of factories owned by the Japanese led to the birth of a new capitalist class that was subordinated to the state. From the viewpoint of the welfare regime, the most significant meaning of the US military government is that it laid the foundations for the developmental state in the 1960s and 1970s in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.112-119
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• 2021

In order to develop a safe seeding technology for low-density transplantation, we analyzed the changes in seeding growth according to different seeding rates and seeding days. We detected changes in leaf number and shoot diameter on the 10th seeding day, and in plant height on the 20th seeding day according to seeding rate. The increase in shoot and leaf number, and shoot diameter per seeding day decreased with anincrease in seeding rate. On seeding day 20, the reductions in shoot and root dry weights according to seeding rate was lower compared with those recorded at seeding day 10. Shoot dry weight was significantly related to root dry weight, plant height, and shoot diameter. With an increase in seeding rate, seeding health decreased and there was an increase in the rate of seeding health per seeding day decreased. For low-density transplantation, the optimal number of seeds required for transplanting 3-5 plants per hill is 11,000, which is estimated to be approximately 280-300 g of seeds for 22 g brown rice. Furthermore, the appropriate number of seeding days is estimated to be 13 to 16 days, which corresponds to a small reduction in seeding health.