Kim, Y.B.;Yoo, J.Y.;Lee, J.Y.;Kim, G.W.;Park, H.Y.
Journal of Animal Science and Technology
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v.46
no.3
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pp.307-314
/
2004
Xenotransplantation of porcine organs has the potential to overcome the severe. shortage of human tissues and organs available for human transplantation. The swine represents an ideal source of such organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human. However, this procedure also carries with a number of safety issues relating to the zoonotic infections. Porcine endogenous retrovinJses(PERVs), \Wich are germ line transmitted and persist without symptoms in the pigs, are most concerning zoonotic viroses. In order to analyze the prevalence of PERV in domestic pigs, four kinds of pigs'(Landrace, Berkshire, Yorkshire, and Duroc) genomic DNA were isolated from their hair follicles. PCR analysis was carried out for detection of PERVs using subgroup A/B/C and E pol sequence primers. All pigs (20 heads) tested had high copy number of PERVs within genomes. Subgroup A/B/C and E pol gene sequences from 20 isolates were determined by direct sequencing. Sequence analysis showed pol sequences are highly conserved among intra- and inter-subspecies(99.l and 98.8%, respectively). As a first report of PERV prevalence in Korea pigs, our data would be the basic concepts of PERV transmission study in xenotransplantation.
Background: Dental hygienists are at risk of acquiring infections through exposure to infectious agents. This study explores the frequency of exposure to infectious agents and infection control practices among dental hygienists. Methods: We undertook a cross-sectional survey to examine the exposure to infectious agents and infection control practices among dental hygienists through questionnaire. Data were obtained from 124 dental hygienists attending educational conferences. A multiple logistic regression analysis was conducted to determine the factors that effect infection control practices in relation to age, completion of infection control education, recognition of their own serum hepatitis Ag/Ab status, the number of one day patients, location of clinics, type of clinics, and career years. Result: Of the 124 dental hygienists, 91.7% were exposed to at least one accident such as needle stick injuries during their practices periods. The health screening coverage rate in dental hygienists was 16.5%. The number of one day patients and career years were important in relation to infection control practices among dental hygienists. The adjusted odds ratio estimates for career years were 5.049 times greater(95% Cl 1.238-20.597) for groups with over 4 career years than under 2 career years. That for the number of one day patients were 0.261(95% Cl 0.071-0.955) lower for through 20 up to 30 patients, 0.531(95% Cl 0.102-2.78) lower for through 30 up to 40 patients and 0.498(95% Cl 0.123-2.017) lower for more than 40 patients than under 20 patients. Conclusion: From these results, prevalence of infection control practices among dental hygienists is related to the number of one day patients and career years. Prevention of cross infection and reduction of future transmission should be a priority to dental hygienists for promotion of infection control and further efforts to educate newcomers on infection prevention should be made.
Kim, Hyun;Chang, Dae-Yung;Rah, Bpng-Jin;Kim, Ho-Dirk
Applied Microscopy
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v.27
no.2
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pp.121-130
/
1997
It has been demonstrated that majority of cells in the mammalian body such as myocytes and epithelial cells of skin and intestine respond to mechanical force or environmental factors and exhibit partial disruption of cell membrane, i. e., cell wounding, even in a physiological condition. Myocardial cells are rather apt to be wounded than other cells since they are definitely exposed to mechanical stress by contraction-relaxation and blood flow. However, the mechanism how myocardial cells protect themselves against cell wounding is not yet clarified. On this background, the present study was performed to elucidate whether albumin leakage is related to cell wounding and to assess whether diltiazem, a potent calcium channel blocker, is beneficial in isoproterenol-induced cell wounding in the heart. Hearts isolated from New Zealand White rabbits ($1.5\sim2.0kg$ body weight, n=20) were perfused with Tyrode solution by Langendorff technique. After stabilization of baseline hemodynamics, the hearts were subjected to bolus administration of isoproterenol and diltiazem as following order: $1.6{\mu}M$ isoproterenol at zero min (the beginning point): $16{\mu}M$ diltiazem at 20min; $1.6{\mu}M$ isoproterenol at 25min; $16{\mu}M$ isoproterenol at 45 min; $160{\mu}M$ diltiazem at 65 min; $16{\mu}M$ isoproterenol at 70 min. During all experiments, the left ventricular function was recorded, albumin leakage in the coronary effluents was analyzed by electrophoresis and Western blot, and myocardial cell membranes were examined by conventional transmission electron microscopy. Data were analyzed by t-test and linear regression test. Isoproterenol significantly increased the inotropic and chronotropic contractions, coronary flow, and frequency of arrhythmia, however, diltiazem did not influence on hemodynamics except decrease in the frequency of arrhythmia and a slight decrease in contractility. Isoproterenol also resulted partial disruption of myocardial cell membrane and inclose in albumin leakage, while diltiazem pretreatment showed number of electron-dense plaques in the cell membrane and a tendency of decrease in albumin leakage. These results indicate that albumin leakage may be an indirect index of cell wounding in the heart and diltiazem nay be beneficial to protect myocardial cells against isoproterenol-induced cell wounding. It is likely that diltiazem promotes resealing process of the cell membrane.
Kim, Seong-Jo;Bahk, Gyung-Jin;Ding, Tian;Kim, Tae-Woong;Oh, Deog-Hwan
Korean Journal of Food Science and Technology
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v.40
no.3
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pp.349-353
/
2008
This study was performed to determine the adherence rates of standard type Salmonella Typhimurium (ST) and wild type Salmonella spp.(WT) in pork after the following contact types and times at $10^{\circ}C$: pork meat (2, 6, 24 hr), conveyer belt (2, 6 hr), stainless steel (2, 6 hr), and cutting board (2, 6 hr). After 6 hr of pork meat to meat fat contact, the adherence rates for ST and WT were approximately 4.21 and 26.87%, respectively, and after 6 hrs of pork meat to red meat contact they were 16.40 and 27.48%, respectively. However, after 24 hr of both types of contact, ST and WT showed 100% adherence rates. The adherence rates for ST after 2 hr and 6 hr of pork meat to conveyer belt contact were 1.34 and 0.60%, respectively, while the adherence rate for WT was 5.14% after 6 hr of contact. After pork meat to stainless steel contact, ST showed adherence rates of zero and 1.59% after 6 and 24 hr of contact, respectively, while the adherence rates for WT ranged from 0.17% after 2 hr to 5.01% after 6 hr. On the other hand, neither ST nor WT offered adherence data following pork meat to cutting board contact. These results suggest that the adherence rates of ST and WT after pork meat to pork meat contact or pork meat to processing surface contact were significantly affected by the contact time and WT presented much higher adherence rates for both types of transmission than ST.
Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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2001.06a
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pp.1210-1210
/
2001
On farm analysis of protein, moisture and oil in cereals and oil seeds is quickly being adopted by Australian farmers. The benefits of being able to measure protein and oil in grains and oil seeds are several : $\square$ Optimize crop payments $\square$ Monitor effects of fertilization $\square$ Blend on farm to meet market requirements $\square$ Off farm marketing - sell crop with load by load analysis However farmers are not NIR spectroscopists and the process of calibrating instruments has to the duty of the supplier. With the potential number of On Farm analyser being in the thousands, then the task of calibrating each instrument would be impossible, let alone the problems encountered with updating calibrations from season to season. As such, NIR technology Australia has developed a mechanism for \ulcorner\ulcorner\ulcorner their range of Cropscan 2000G NIR analysers so that a single calibration can be transferred from the master instrument to every slave instrument. Whole grain analysis has been developed over the last 10 years using Near Infrared Transmission through a sample of grain with a pathlength varying from 5-30mm. A continuous spectrum from 800-1100nm is the optimal wavelength coverage fro these applications and a grating based spectrophotometer has proven to provide the best means of producing this spectrum. The most important aspect of standardizing NIB instruments is to duplicate the spectral information. The task is to align spectrum from the slave instruments to the master instrument in terms of wavelength positioning and then to adjust the spectral response at each wavelength in order that the slave instruments mimic the master instrument. The Cropscan 2000G and 2000B Whole Grain Analyser use flat field spectrographs to produce a spectrum from 720-1100nm and a silicon photodiode array detector to collect the spectrum at approximately 10nm intervals. The concave holographic gratings used in the flat field spectrographs are produced by a process of photo lithography. As such each grating is an exact replica of the original. To align wavelengths in these instruments, NIR wheat sample scanned on the master and the slave instruments provides three check points in the spectrum to make a more exact alignment. Once the wavelengths are matched then many samples of wheat, approximately 10, exhibiting absorbances from 2 to 4.5 Abu, are scanned on the master and then on each slave. Using a simple linear regression technique, a slope and bias adjustment is made for each pixel of the detector. This process corrects the spectral response at each wavelength so that the slave instruments produce the same spectra as the master instrument. It is important to use as broad a range of absorbances in the samples so that a good slope and bias estimate can be calculated. These Slope and Bias (S'||'&'||'B) factors are then downloaded into the slave instruments. Calibrations developed on the master instrument can then be downloaded onto the slave instruments and perform similarly to the master instrument. The data shown in this paper illustrates the process of calculating these S'||'&'||'B factors and the transfer of calibrations for wheat, barley and sorghum between several instruments.
This paper presents an algorithm for multichannel slotted-ring topology medium access protocol (MAC) using in wavelength division multiplexing (WDM) networks. In multichannel ring, there are two main previously proposed architectures: Tunable Transmitter - Fixed Receiver (TTFR) and Fixed Transmitter - Tunable Receivers (FTTR). With TTFR, nodes can only receive packets on a fixed wavelength and can send packets on any wavelengths related to destination of packets. Disadvantage of this architecture is required as many wavelengths as there are nodes in the network. This is clearly a scalability limitation. In contrast, FTTR architecture has advantage that the number of nodes can be much larger than the number of wavelength. Source nodes send packet on a fixed channel (or wavelength) and destination nodes can received packets on any wavelength. If there are fewer wavelengths than there are nodes in the network, the nodes will also have to share all the wavelengths available for transmission. However the fixed wavelength approach of TTFR and FTTR bring low network utilization. Because source node with waiting data have to wait for an incoming empty slot on corresponding wavelength. Therefore this paper presents Tunable Transmitter - Tunable Receiver (TTTR) approach, in which the transmitting node can send a packet over any wavelengths and the receiving node can receive a packet from any wavelengths. Moreover, the self-similar distributed input traffic is used for evaluation of the performance of the proposed algorithm. The self-similar traffic performs better performance over long duration than short duration of the Poison distribution. In order to increase bandwidth efficiency, the Destination Stripping approach is used to mark the slot which has already reached the desired destination as an empty slot immediately at the destination node, so the slot does not need to go back to the source node to be marked as an empty slot as in the Source Stripping approach. MATLAB simulator is used to evaluate performance of FTTR, TTFR, and TTTR over 4 and 16 nodes ring network. From the simulation result, it is clear that the proposed algorithm overcomes higher network utilization and average throughput per node, and reduces the average queuing delay. With future works, mathematical analysis of those algorithms will be the main research topic.
The objective of the present study is to provide data needed to decide covering method to be able to increase the thermal insulation and light transmittance efficiency of commercial greenhouse. The thermal insulation effect, PPF transmittance and quantity of condensation water were estimated in experimental tomato greenhouses covered with three types of coverings of single layer, air inflated and conventional double layers covering. The overall heat flow of air inflated double layers greenhouse was similar to that of conventional double layers greenhouse, but the temperature between covering material and thermal screen in air inflated double layers greenhouse was lower than that in conventional double layers greenhouse at the same outside temperature condition due to air leakage through the gap of roof vent. The overall heat transfer coefficients acquired by experiment that was performed in single layer and conventional double layers greenhouses were close to those obtained from model experiment. Even though the PPF transmittance of air inflated double layers greenhouse was lower than that of single layer greenhouse, which was greater than that of conventional double layers greenhouse. The quantity of condensation water on covering surface of single layer greenhouse was greater than that of air inflated double layers greenhouse due to lower covering surface temperature.
The Journal of Korean Institute of Electromagnetic Engineering and Science
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v.27
no.12
/
pp.1084-1096
/
2016
This paper presents method of design and analysis of a high-speed serial interface connector with a data rate of 12.5 Gbps. A high-speed serial interface connector is composed of various material and complex structures. It is very difficult to match the impedance of each discontinuous portion of connector. Therefore, this paper proposes the structure of a connector line that be simplified a connector. In the structure of proposed connector line, this research presents a method for extracting R, L, C and G parameters, analyzing the differential mode impedance, and minimizing the impedance discontinuity using time domain transmissometry and time domain reflectometry. This paper applies the proposed methods in the connector line to the high-speed serial interface connector. The proposed high-speed serial interface connector, which consists of forty-four pins, is analyzed signal transmission characteristics by changing the width and spacing of the four pins. According to the analysis result, as the width of the ground pin increases, the impedance decreases slightly. And as the distance between the ground pin and the signal pin increases, the impedance increases. In addition, as the width of the signal pin increases, the impedance decreases. And as the distance between the signal pin and the signal pin increases, the impedance decreases. The impedance characteristic of initial connector presents ranges from 96 to $139{\Omega}$. Impedance characteristic after applying the structure of proposed connector is shown as a value between 92.6 to $107.5{\Omega}$. This value satisfies the design objective $100{\Omega}{\pm}10%$.
Surface soils on Barton Peninsula, King George Island, West Antarctica were investigated to acquire the mineralogical and geochemical data of soil in Antarctica. Multiline of techniques for example, X-ray diffraction (XRD), transmission electron microscopy (TEM)-electron energy loss spectroscopy (EELS), and wet chemistry analysis were performed to measure the composition of clay minerals, Fe-oxidation states, cation exchange capacity, and total cation concentration. Various minerals in sediments such as smectite, illite, chlorite, kaolinite, quartz and plagioclase were identified by XRD. Fe-oxidation states of bulk soils showed 20-40% of Fe(II) which would be ascribed to the reduction of Fe in clays as well as Fe-bearing minerals. Moreover, redox states of Fe in smectite structure was a ~57% of Fe(III) consistent to the values for the bulk soils. The cation exchange capacity of bulk soils ranged from 100 to 300 meq/kg and differences were not significantly measured for the sampling locations. Total cations (Mg, K, Na, Al, Fe) of bulk soils varies, contrast to the heavy metals (Co, Ni, Cu, Zn, Mn). These results suggested that composition of bed rocks influenced the distribution of elements in soil environments and soils containing clay compositions may went through the bio/geochemical alteration.
Journal of the Korea Institute of Information Security & Cryptology
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v.17
no.2
/
pp.125-137
/
2007
This paper proposes a new scheme to provide the location privacy of sources in Wireless Sensor Networks (WSNs). Because the geographical location of a source sensor reveals contextual information on an 'event' in WSN, anonymizing the source location is an important issue. Despite abundant research efforts, however, about data confidentiality and authentication in WSN, privacy issues have not been researched well so far. Moreover, many schemes providing the anonymity of communication parties in Internet and Ad-hoc networks are not appropriate for WSN environments where sensors are very resource limited and messages are forwarded in a hop-by-hop manner through wireless channel. In this paper, we first categorize the type of eavesdroppers for WSN as Global Eavesdropper and Compromising Eavesdropper. Then we propose a novel scheme which provides the anonymity of a source according to the types of eavesdroppers. Furthermore, we analyze the degree of anonymity of WSN using the entropy-based modeling method. As a result, we show that the proposed scheme improves the degree of anonymity compared to a method without any provision of anonymity and also show that the transmission range plays a key role to hide the location of source sensors.
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