• Journal of Advanced Marine Engineering and Technology
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• v.40 no.2
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• pp.120-130
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• 2016

In general, a series of ship steering motions is composed of a combination of translational motions and rotational motions of the ship. In particular, a series of rotational motions frequently occurs in narrow areas such as ports and canal zones. In this paper, a method was suggested for composing an integrated control algorithm based on the jog dial as a command instrument for rotational motion control. In order to realize the rotational motions, several algorithms were suggested for generating rotational commands, for selecting motion variables, for choosing reference input values for the motion variables, for computing required accelerations and thrusts, and for allocating thrusts to actuators. A simulation program was compiled to execute simulations for three rotational motions. Finally, the effectiveness of the suggested method was verified by analyzing the simulation results.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.8
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• pp.38-45
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• 1999

The 2-trowel type concrete floor finishing robot can move in any direction by adjusting the posture or trowels without any wheels. Since the quality of the smoothed and polished concrete floor is determined by plastering speed, we need to control the velocity of the robot. However, we cannot use the typical motion control method because it is very difficult to measure the velocity of the robot, in contrast to the mobile robots with wheels. To overcome this difficulty, the following are studied in this paper: we found that the robot dynamics has the disturbance depending on its translational speed, and showed that there exists the saturated velocity of the robot which is set by the posture of the trowels, and obtained the relationship between the saturated velocity and the posture in the translation. The result enables us to control the motion of the robot only by adjusting the posture of trowels without measuring the velocity of the robot. Currently, we built the troweling robot and are experimenting its performance with the proposed motion control method.

• Molecules and Cells
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• v.44 no.11
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• pp.784-794
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• 2021

Leiomyosarcoma (LMS) is a mesenchymal malignancy with a complex karyotype. Despite accumulated evidence, the factors contributing to the development of LMS are unclear. Here, we investigated the role of tight-junction protein 1 (TJP1), a membrane-associated intercellular barrier protein during the development of LMS and the tumor microenvironment. We orthotopically transplanted SK-LMS-1 cells and their derivatives in terms of TJP1 expression by intramuscular injection, such as SK-LMS-1 Sh-Control cells and SK-LMS-1 Sh-TJP1. We observed robust tumor growth in mice transplanted with LMS cell lines expressing TJP1 while no tumor mass was found in mice transplanted with SK-LMS-1 Sh-TJP1 cells with silenced TJP1 expression. Tissues from mice were stained and further analyzed to clarify the effects of TJP1 expression on tumor development and the tumor microenvironment. To identify the TJP1-dependent factors important in the development of LMS, genes with altered expression were selected in SK-LMS-1 cells such as cyclinD1, CSF1 and so on. The top 10% of highly expressed genes in LMS tissues were obtained from public databases. Further analysis revealed two clusters related to cell proliferation and the tumor microenvironment. Furthermore, integrated analyses of the gene expression networks revealed correlations among TJP1, CSF1 and CTLA4 at the mRNA level, suggesting a possible role for TJP1 in the immune environment. Taken together, these results imply that TJP1 contributes to the development of sarcoma by proliferation through modulating cell-cell aggregation and communication through cytokines in the tumor microenvironment and might be a beneficial therapeutic target.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.664-669
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• 2003

This paper proposes a swing-arm type dual-stage actuator, which consists of a PZT actuator for fine motion and a VCM(Voice Coil Motor) for coarse motion, for SFF ODD(Small Form Factor Optical Disk Drive), in order to achieve fast access speed and precise track following control. We focus our attention on the design and control of the PZT actuator, because there have been a lot of previous researches related to the VCM and dual-stage actuators. Due to the dual cantilever structure, the PZT actuator can generate precise translational tracking motion at its tip where optical pickup is attached at, and the effect of hysteric behavior of the PZT element is reduced. The dynamic model of the PZT actuator is derived by using the Hamilton's principle, and verified by comparing with the experimental frequency response. The sliding mode control is designed in order to be robust against modeling uncertainties. Simulations and experimental results confirm the effectiveness of the suggested control scheme.

• Journal of KSNVE
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• v.3 no.4
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• pp.373-382
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• 1993

Active micro-vibration control of a structure, which simulates a stepper device, is performed using a pair of piezolectric actuators. The control aims at reducing the translational and rotational vibrations of the upper plate when the base is subject to seismic disturbance and the upper plate undergoes impulsive transient motion. Using the experimentally determined model, derivative control scheme is adopted so that the damping of the closed-loop system is effectively increased. It is found that the predicted control performance is in good agreement with the experimental results. Finally, the limit cycle phenomenon due to the controller voltage saturation is compared with the simulation.

• Analytical Science and Technology
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• v.8 no.4
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• pp.881-886
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• 1995

Several forms of gangliosides have been separated from various types of biological matrices using cyclodextrin-modified capillary zone electrophoresis (CZE). Quantitative analysis of phospholipids from biological fluids was achieved by micellar electrokinetic capillary chromatography (MECC) using 35mM sodium dodecyl sulfate. Phosphorylation, one of the most important post-translational modifications of proteins at serine, threonine and tyrosine residues in small peptides were identified and quantitative analyses of phosphopeptides were performed. Seven different neuropeptides which are relative the pain reachanism in the vertebrate central nervons system were also separated by CZE.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.38
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• pp.17.1-17.6
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• 2016

Background: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Methods: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05). Conclusions: These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.

• Coupled systems mechanics
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• v.5 no.2
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• pp.191-201
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• 2016

Coupled structures are widely seen in civil and mechanical engineering. In coupled structures, monitoring the translational motion of its key components is of great importance. For instance, some coupled arms are equipped with a hydraulic piston to provide the stiffness along the piston axial direction. The piston moves back and forth and a distance sensing system is necessary to make sure that the piston is within its stroke limit. The measured motion data also give us insight into how the coupled structure works and provides information for the design optimization. This paper develops two distance sensing systems for coupled structures. The first system measures distance with ultrasonic sensor. It consists of an ultrasonic sensing module, an Arduino interface board and a control computer. The system is then further upgraded to a three-sensor version, which can measure three different sets of distance data at the same time. The three modules are synchronized by the Arduino interface board as well as the self-developed software. Each ultrasonic sensor transmits high frequency ultrasonic waves from its transmitting unit and evaluates the echo received back by the receiving unit. From the measured time interval between sending the signal and receiving the echo, the distance to an object is determined. The second distance sensing system consists of a wire draw encoder, a data collection board and the control computer. Wire draw encoder is an electromechanical device to monitor linear motion by converting a central shaft rotation into electronic pulses of the encoder. Encoder can measure displacement, velocity and acceleration simultaneously and send the measured data to the control computer via the data acquisition board. From experimental results, it is concluded that both the ultrasonic and the wire draw encoder systems can obtain the linear motion of structures in real-time.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4689-4693
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• 2012

Lung cancer is the most common type of cancer and one of the leading causes of death in the world. Genetic factors play an important role in its development. PDCD6, the encoding gene for programmed cell death protein 6, may function as a tumor suppressor gene. Non-small cell lung cancer (NSCLC) contributes about 80% to newly histologically diagnosed lung cancer patients. To explore the relationship between PDCD6 and NSCLC, we examined two single nucleotide polymorphisms(rs3756712 G/T andrs4957014 G/T, both in the intron region) of the PDCD6gene.A hospital-based case-control study was carried out including 302 unrelated NSCLC patients and 306 healthy unrelated subjects. Significantly increased NSCLC risk was found to be associated with the T allele of rs4957014 (P=0.027, OR=0.760, 95%CI=0.596-0.970). The genotype and allele frequencies of rs3756712 did not shown any significant difference between NSCLC group and controls (P=0.327, OR=0.879, 95%CI=0.679-1.137). In conclusion, we firstly demonstrated the association between the PDCD6 gene and risk of NSCLC in a Chinese Han population.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.277-283
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• 2015

Plant genome analyses, including Arabidopsis thaliana showed a large gene family of plant receptor kinases with various extracellular ligand-binding domain. Now intensively studies to understand physiological and cellular functions for higher plant receptor kinases in diverse and complex biological processes including plant growth, development, ligands perception including steroid hormone and plant-microbe interactions. Brassinosteroids (BRs) as a one of well know steroid hormone are plant growth hormones that control biomass accumulation and also tolerance to many biotic and abiotic stress conditions and hence are of relevance to agriculture. BRI1 receptor kinase, which is localized in plasma membrane in the cell sense BRs and it bind to a receptor protein known as BRASSINOSTEROID INSENSITIVE 1 (BRI1). Recently, we reported that BRI1 and its co-receptor, BRI1-ASSOCIATED KINASE (BAK1) autophosphorylated on tyrosine residue (s) in vitro and in vivo and thus are dual-specificity kinases. Other plant receptor kinases are also phosphorylated on tyrosine residue (s). Post-translational modifications (PTMs) can be studied by altering the residue modified by directed mutagenesis to mimic the modified state or to prevent the modification. These approaches are useful to not only characterize the regulatory role of a given modification, but may also provide opportunities for plant improvement.