• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.491-492
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• 2010

• 한국식물학회:학술대회논문집
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• 한국식물학회 2002년도 춘계학술발표대회:발표눈문요지록
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• pp.112-112
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• 2002

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.234-234
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• 2022

Gene-editing is currently one of the most popular technologies in recent years. Development of the new crop using the gene editing have advantage of improved accuracy and efficiency compared with conventional breeding. Soybean (Glycine max L.) is one of the most important crops worldwide used as food and forage. We tried to establish a system for breeding improvement of soybean through gene-editing technology. For the gene-editing system of soybean, i) selection of efficiency gRNA of targeted gene, ii) efficient genetic transformation of the selected gRNA, iii) selection of trans-clean mutant is essential. First of all, we investigated the selection conditions of gRNA with high editing efficiency of targeted gene using isolated protoplast of soybean. Furthermore, we performed the Agrobacterium-mediated genetic transformation of various soybean cultivars. We identified the tissue culture ability in 23 soybean cultivars for genetic transformation of soybean. The six cultivars with high tissue culture ability were selected and confirmed the transgenic plants in four cultivars. Finally, we established a speed-breeding system as a powerful tool for the fast selection of trans-clean mutants from transgenic plants. Our laboratory will provide the valuable system for improvement of soybean by the gene-editing technology.

• Nuclear Engineering and Technology
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• 제16권1호
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• pp.29-35
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• 1984

유리섬유 여과포에 의한 0.1~0.45$\mu\textrm{m}$ 크기의 DOP 부유입자의 제거효율시험을 1~10cm/sec의 유속범위에서 수행하였다. 섬유여과포에 의한 미세입자의 여과는 여과속도에 의하여 결정되는데, 최소제거 효율을 나타내는 DOP 입자의 크기는 여과속도가 증가함에 따라 감소한다. Kuwabara Cell Model과 Von Mises Transformation을 사용하여 유리섬유 여과포내에서 화산에 의한 입자 제거효율을 수치해석적으로 전개하여 구하였으며, 이 계산치는 실험치와 잘 일치하였다. 본 실험의 결과는 여과포의 입자제거효율을 예측하고 최적 운전조건을 추구하는데 기여할 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제50권
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• pp.56-62
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• 2023

35S cauliflower mosaic virus 프로모터의 조절을 받고 인트론이 포함된 β-Glucuronidase (gus) gene과 35S cauliflower mosaic virus (enhanced) 프로모터의 조절을 받는 blpR유전자가 있는 pCAMBIA3301 벡터가 포함된 AGL-1 균주를 사용하였다. 아그로박테리움을 이용한 형질전환 체계와 PPT (D-L-phosphinothricin) 선발을 통하여 나리 인편조직으로부터 형질전환 식물체가 획득되었다. 본 연구에서 나리 레드플레임'품종의 인편조직에 선발 및 목적유전자로 바스타 제초제저항성 유전자인 blpR 유전자를 도입하였다. 상기 실험 결과, 20분의 접종시간과 5일간의 아그로박테리움과의 공동배양이 100개의 접종된 인편개체에서 각각 24, 27개의 높은 PPT 저항성 개체가 관찰되었고 신초까지 형성된 인편을 19.6 및 22.7개를 생산하는 우수한 형질전환 결과를 보여주었다. 이렇게 제초제를 이용하여 선발되었을 뿐만 아니라 도입된 reporter 유전자인 gus도 발현되었음을 확인하였고 선발유전자이자 목적유전자인 blpR 유전자도 PCR 검정을 통해 도입되었음을 확인하였다. 12주 이상의 선발과정을 거치고 gus 및 PCR 검정을 거친 형질전환 개체들은 발근 배지를 거쳐 순화 후 화분으로 이식하여 높은 활착율을 보여주었다. 결론적으로 본 연구에서 확립한 프로토콜을 이용하면 평균 20% 이상의 형질전환 효율을 나타내고 본 연구에 기술된 아그로박테리움 매개 형질전환 체계에 향후 보완이 필요하지만, 우수 품종개발을 위한 나리 육종 프로그램에 기여할 수 있을 것으로 판단된다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 추계학술대회논문집
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• pp.809-814
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• 2006

In this paper, the modified direct method employing beam transformation technique is proposed in order to efficiently calculate hydroelastic responses of floating structure. Since the proposed method expresses the displacements of three-dimensional structure with those of transformed beam which leads to small number of equations of motion, the method is numerically efficient compared to the conventional direct method. To verify the efficiency of the proposed method a 500 m-long floating structure under wave loads is considered in numerical example. Displacements, bending moments, torsion moments and shear forces are calculated and computing tine is examined. The results are also compared with those of the conventional direct method.

• 대한기계학회논문집A
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• 제28권1호
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• pp.70-78
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• 2004

This paper presents study of efficiency of wavelet transformation for on-line chatter detection during hard fuming process. From comparison with other time series and statistical methods such as fast fourier transformation (FFT), Kurtosis and standard deviation (STD), wavelet transform is better than others in on-line chatter detection. With using wavelet function with pseudo frequency corresponding to chatter frequency, chatter could be detected more sensitively. And for both force signal from dynamometer and displacement signal from capacitance type cylindrical sensor (CCS), wavelet transform with DB2 function on level 4 could be well used for chatter detection in hard turning process.

• 대한수학회보
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• 제51권2호
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• pp.595-612
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• 2014

The spectral collocation method for a second order elliptic boundary value problem on a domain ${\Omega}$ with curved boundaries is studied using the Gordon and Hall transformation which enables us to have a transformed elliptic problem and a square domain S = [0, h] ${\times}$ [0, h], h > 0. The preconditioned system of the spectral collocation approximation based on Legendre-Gauss-Lobatto points by the matrix based on piecewise bilinear finite element discretizations is shown to have the high order accuracy of convergence and the efficiency of the finite element preconditioner.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2011년 춘계학술대회논문집
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• pp.261-265
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• 2011

An adaptive wavelet transformation method with high order accuracy is proposed to allow efficient and accurate flow computations. While maintaining the original numerical accuracy of a conventional solver, the scheme offers efficient numerical procedure by using only adapted dataset. The main algorithm includes 3rd order wavelet decomposition and thresholding procedure. After the wavelet transformation, 3rd order of spatial and temporal accurate high order interpolation schemes are executed only at the points of the adapted dataset. For the other points, high order of interpolation method is utilized for residual evaluation. This high order interpolation scheme with high order adaptive wavelet transformation was applied to unsteady Euler flow computations. Through these processes, both computational efficiency and numerical accuracy are validated even in case of high order accurate unsteady flow computations.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2010년도 춘계학술발표대회
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• pp.39.2-39.2
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• 2010

Recently, hot stamping process has been paid attention greatly by automobile makers in accordance with the fuel efficiency and environmental issues as well as crash safety issue. The hot-stamped parts, however, demand extreme mechanical properties such as tensile strength of over 1470 MPa or equivalently Vickers hardness of around 450. In this work, to meet the demand efficiently, a method to predict mechanical property of hot-stamped parts based on numerical phase transformation scheme has been proposed associated with the thermo-mechanical coupled finite element analysis. This work deals with various phase transformation equations and validates them to select appropriate model for 0.2C-0.1Si-1.4Mn-0.5Cr-0.01Mo-0.002B steel sheet. The authors show that an efficient method saving time and cost to develop hot-stamped automobile parts ensuring suitable mechanical properties such as Vickers hardness and strength.