• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.11
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• pp.1410-1420
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• 2005

The purpose of this study was to analyze characteristics of effective expression on T-shirts pattern by peirce's semiotic theory. The methods of this study was to analyze 721 T-shirts patterns in designer's collection from 2000 to 2004. The results of this study were as follows: First of all, iconic expression showed high frequency after 2000. Characteristics of effective expression were as follows: In geometric expression, repetition was presented as a symbolic sign by regular proportion and rule of dot, line, plane, irregularity, mixture of irregular dot, line, plane. Mixed expression was presented as a iconic and symbolic sign by collage, mixture of dot, line, plane, icon and letter. In iconic expression, simplification of iconic sigrl was presented as a iconic sign by simplification of form, color, texture, realistic expression using digital as a iconic sign, and symbolic face, body as a symbolic sign. Pop art's expression was presented as a iconic sign by a cartoon and commercial character and illusion was presented as a iconic sign. In letter's expression, brand logo was presented as a symbolic sign by transformation of letter's design, a symbolic sign of numeral by transformation of size, thickness, form, color. Symbolic message phrase was presented by slogan, fashion trend, brand image, descriptive indication message as a index sign by using icon or singleness. In conclusion, characteristics of effective expression on T-shirts pattern will present not only the theoretical foundation to raise the value added, but also the information about beauty sense of times, political and social value.

• Journal of Plant Biotechnology
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• v.4 no.3
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• pp.129-134
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• 2002

We report the development of a new selection system for the transformation of pepper plants by mannose. In order to achieve this, we first tested several factors related to regeneration conditions. Among the 30 inbred lines examined, line P9l5 was able to generate shoots at the highest rate from both cotyledons and hyporotyls in MS media. A dosage curve for optimizing the selection conditions was established by mixing mannose (range 0-50 g/L) and sucrose (range 0-30 g/L). The least selection pressure on shoot formation was created by a mixture of sucrose and mannose at 20 g/L and 15 g/L, respectively, and the threshold for ultimate tissue death was 50 g/L of mannose irrespective of the sucrose concentration. However, we found that mannose itself was not the sole inhibitor of pepper shoot development. High concentrations of sucrose (30 g/L) contributed additively to the inhibition of shoot formation at higher mannose concentrations. Genotype preference is a major factor that enhances regeneration ability in mannose media, as was observed in MS media. P9l5 and P410 line had high regeneration rates under mannose selection conditions in the presence of Agrobacterium infection. Different virulence levels of Agrobacterium strains did change the regeneration rates, probably due to interaction with the specificities of the inbred lines. Taken together, P9l5 offers the best pepper inbred line for transformation and we recommend a selection condition of 20 g/L of sucrose and 15 g/L or more of mannose up to 50 g/L in media.

• Journal of the Korean Home Economics Association
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• v.34 no.2
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• pp.43-55
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• 1996

The purpose of this study was to investigate the slacks pattern making on the movement-fitness of the knee region. The experimental items were divided into the lower limb movement (M1~M5) and slacks pattern designs (4 : basic pattern, in-pleats, side-pleats, seam-line increase). This study was done by clothing pressure test, sensory evaluation test and the difference shape-transformation of wearing-slacks. The summarized finding resulted from experiments and investigation are suggested as follows ; First, the clothing pressure test showed that the order of clothing pressure in the movement was the rabbit leap movement (M3), the sit on knees (M4), the nobleman sitting (M5), the sit on chair (M2) from the highest to the lowest. In the comparison of clothing pressure tested by blocks of lower-limb, center front knee region shows the highest of 300g/cm2. In it by constructional methods, the clothing pressure of in-pleats, side-pleats methods shows lower than basic pattern, hem-line increase methods. Also in comparison of the two methods, in-pleats method shows lower than side-pleats method. Second, by the sensory evaluation test based on the lower-limb movements and constructional methods of slacks, it was found that the functional factor score of the rabbit leap movement was also the waist, hip, knee region was the lowest in all blocks. And in the comparison of constructional methods, in-pleats method was the highest in all methods. Third, in consideration of the difference in the shape-transformation of wearing-slacks on the movement-fitness, pleats pattern design methods showed that the ratio of shape-transformation was lower than basic on seam-line increase methods. But the difference of the side-pleats and in-pleats methods was litle. According to these investigation, the slacks pattern making caused by movement-fitness on the knee region, in-pleats design method was the functional slacks pattern.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.13 no.3
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• pp.257-262
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• 2002

A single-layered radial line slot array (SL-RLSA) antenna etched on a substrate and fad by a rectangular waveguide is presented in the 60 ㎓ band. The design curves are obtained by an efficient electromagnetic coupling analysis using Ewald Sum technique and Shanks transformation. The antenna has rectangular waveguide feed structure using a rectangular waveguide-to-radial line transition. The prototype antenna of 10 cm-diameter was tested and the gain of 31 ㏈i and the efficiency of 38% were measured at 60 ㎓.

• Proceedings of the KSRS Conference
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• v.2
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• pp.856-859
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• 2006

In river with bar, the characteristics of its physical conditions have a close relationship with bar morphology. In this paper, a monitoring approach of bar transformation in the Han River Estuary is presented using RADARSAT/SAR Images. The estuary is divided into North and South Korea and its area is blocked by CCL(Civil Control Line). Satellite remote sensing, therefore, is uniquely suited to monitoring bar transformation. Based on SAR signatures for bars, bar transformation is investigated from 2000 to 2005, and monitoring of suspended-silt transportations from terrestrial runoff is tried to understand the morphology during the events of severe rain storm. SAR data did not reveal clearly the bar locations because of most of data acquisitions during high tides from 6.8 m to 9.0 m. Even though the problem, it could be said that in the estuary vegetated area and natural levees are developed well, but bars and shifted after an event like a flood. It is also showed that suspended solids such as silt transported through the estuary could contribute highly to a sedimentation environment around Incheon.

• Journal of Marine Bioscience and Biotechnology
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• v.16 no.1
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• pp.26-35
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• 2024

Chlorella has various biotechnological applications, including in the biomedical and pharmaceutical industries, because of its advantages, including rich nutrients, fast growth rate, easy cultivation, and high biomass. We used the Agrobacterium-mediated transformation method to express human GM-CSF and EGF proteins, which are widely used in regenerative medicine, cosmetics, and pharmaceutical materials in Chlorella. The codon-optimized hGM-CSF and hEGF genes were cloned into plant binary vectors and transformed into Chlorella vulgaris using the Agrobacterium-mediated coculture transformation method. After transformation, genomic DNA PCR was performed for each C. vulgaris line that was stably subcultured on an antibiotic-resistant solid medium to confirm the insertion of hGM-CSF and hEGF into the chromosome. Furthermore, PT-PCR and protein expression of hGM-CSF and hEGF in each transformed C. vulgaris were significantly increased compared to the untransformed Chlorella. This study suggests that high-value proteins, including hGM-CSF and hEGF, which are foreign genes of C. vulgaris, can be stably expressed through the Agrobacterium-mediated Chlorella transformation system.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.293-301
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• 2010

The feasibility of two strategies for transgene pyramiding using Agrobacterium-mediated transformation was investigated to develop a transgenic potato (Solanum tuberosum L. cv. Iwa) with resistance to potato tuber moth (PTM) (Phthorimaea operculella (Zeller)). In the first approach, cry1Ac9 and cry9Aa2 genes were introduced simultaneously using a kanamycin (nptII) selectable marker gene. The second approach involved the sequential introduction (re-transformation) of a cry1Ac9 gene, using a hygromycin resistance (hpt) selectable marker gene, into an existing line transgenic for a cry9Aa2 gene and a kanamycin resistance (nptII) selectable marker gene. Multiplex polymerase chain reaction (PCR) confirmed the presence of the specific selectable marker gene and both cry genes in all regenerated lines. The relative steady-state level of the cry gene transcripts in leaves was quantified in all regenerated lines by real-time PCR analysis. Re-transformation proved to be a flexible approach to effectively pyramid genes for PTM resistance in potato, since it allowed the second gene to be added to a line that was previously identified as having a high level of resistance. Larval growth of PTM was significantly inhibited on excised greenhouse-grown leaves in all transgenic lines, although no lines expressing both cry genes exhibited any greater resistance to PTM larvae over that previously observed for the individual genes. It is anticipated that these lines will permit more durable resistance by delaying the opportunities for PTM adaptation to the individual cry genes.

• Journal of the Korean Institute of Telematics and Electronics
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• v.12 no.2
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• pp.11-17
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• 1975

The paper examined the character structure as a basic study for the recognition of Korean characters. In view of concave structure, line structure and node relationship of character graph, the algebraic structure of the basic Korean characters is are analized. Also, the degree of complexities in their character structure is discussed and classififed. Futhermore, by describing the fact that some equivalence relations are existed between the 10 vowels of rotational transformation group by Affine transformation of one element into another, it could be pointed out that the geometrical properting in addition to the topological properties are very important for the recognition of Korean characters.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.61-68
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• 2004

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains $5\textrm{mgL}^{-1}$ kinetin, $0.5\textrm{mgL}^{-1}$ NAA, 2 % sucrose (or maltose), 3% sorbitol, and $500\textrm{mgL}^{-1}$ proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

• Korean Journal of Remote Sensing
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• v.28 no.1
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• pp.55-67
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• 2012

Real-time image georeferencing is required to generate spatial information rapidly from the image sequences acquired by multi-sensor systems. To complement the performance of position/attitude sensors and process in real-time, we should employ on-line aerial triangulation based on a sequential estimation algorithm. In this study, we thus attempt to derive an efficient on-line aerial triangulation algorithm for real-time georeferencing of image sequences. We implemented on-line aerial triangulation using the existing Given transformation update algorithm, and a new inverse normal matrix update algorithm based on observation classification, respectively. To compare the performance of two algorithms in terms of the accuracy and processing time, we applied these algorithms to simulated airborne multi-sensory data. The experimental results indicate that the inverse normal matrix update algorithm shows 40 % higher accuracy in the estimated ground point coordinates and eight times faster processing speed comparing to the Given transformation update algorithm. Therefore, the inverse normal matrix update algorithm is more appropriate for the real-time image georeferencing.