• Title/Summary/Keyword: Transfer protocols

Search Result 185, Processing Time 0.032 seconds

Nonviral Vector for Efficient Gene Transfer to Human Ovarian Adenocarcinoma Cells

  • Kim, Chong-Kook;Haider, K.H.;Choi, Sung-Hee;Choi, Eun-Jeong;Ahn, Woong-Shick
    • Proceedings of the PSK Conference
    • /
    • 2002.10a
    • /
    • pp.426.2-426.2
    • /
    • 2002
  • Various strategies have been attempted to design efficient protocols for ovarian cancer gene therapy but there has been little progress in their clinical application. In this study, we formulated and evaluated a new cationic liposome composed of dioleoyltrimethylaminopropane (DOTAP), 1.2-dioleoyl-3-phosphophatidylethanolamine (DOPE). and cholesterol (Chol) (DDC) for plasmid DNA transfer into ovarian cancer cells. The DOC liposome was prepared by mixing DOTAP. DOPE. and Chol using extrusion method. (omitted)

  • PDF

Automatic Interface Synthesis based on IP Categorization and Characteristics Matching (IP 범주화와 특성 대응을 통한 인터페이스 회로 자동 합성)

  • Yun, Chang-Ryul;Jhang, Kyoung-Son
    • Journal of the Institute of Electronics Engineers of Korea SD
    • /
    • v.43 no.10 s.352
    • /
    • pp.34-44
    • /
    • 2006
  • A system-on-a-chip (SoC) design uses pre-verified IP hardware blocks in order to reduce design time. We need interface circuits to connect IPs with different protocols. In SoC design we should design interface circuits frequently and these tasks are somewhat time-consuming and error-prone. So it is necessary to generate the interface circuits automatically. Several studies have been made on generating interface circuits only from the communication protocols of IPs. With existing approaches, it is not easy to generate interface circuits connecting two IPs only from communication protocols: connection between IP with address and W without address, connection between IP with only one port to transfer address/data and IP with different ports for address and data connection between IP that transfer address and data together and IP that transfer only one address with a number of data in a burst. No consideration of various characteristics of IPs and no changed algorithm are responsible for it. In order to solve this problem, the proposed approach categorizes communication protocols of IPs, and takes characteristics matching of IPs into account during the interface synthesis. In experiments, we show that we could correctly generate and verify interface circuits for IPs with different characteristics.

RFID Tag Ownership Relocation Protocol Based on Trusted Third Party (신뢰받는 제3자 기반의 RFID 태그 소유권 이전 프로토콜)

  • Kim, Young-Sik
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.40 no.3
    • /
    • pp.568-574
    • /
    • 2015
  • Recently RFID not only is widely utilized in various fields such as inventory management, merchandize logistics, etc., but also, has evolved as an important component of the Internet of Things (IoT). According to increasing the utilization field of RIFD, studies for security and privacy for RFID system have been made diverse. Among them, the ownership transfer protocols for RFID tags have also been proposed in connection with the purchase of products embedded with RFID tag. Recently, Kapoor and Piramuthu proposed a RFID ownership transfer protocol to solve the problems of security weakness of the previous RFID ownership transfer protocols. In this paper, we show that Kapoor-Piramuthu's protocol also has security problems and provide a new protocol to resolve them. Security analysis of newly proposed protocol shows the security concerns are resolved.

Design and Implementation of a CAN Data Analysis Test Bench based on Raspberry Pi

  • Pant, Sudarshan;Lee, Sangdon
    • Journal of Multimedia Information System
    • /
    • v.6 no.4
    • /
    • pp.239-244
    • /
    • 2019
  • With the development of Cyber-Physical Systems(CPS), several technologies such as automation control, automotive and intelligent house systems have been developed. To enable communication among various components of such systems, several wired and wireless communication protocols are used. The Controller Area Network(CAN) is one of such wired communication protocols that is popularly used for communication in automobiles and other machinery in the industry. In this paper, we designed and implemented a response time analysis system for CAN communication. The reliable data transfer among various electronic components in a significant time is crucial for the smooth operation of an electric vehicle. Therefore, this system is designed to conveniently analyze the response time of various electronic components of a CAN enabled system. The priority for transmission of the messages in the CAN bus is determined by the message identifier. As the number of nodes increases the transmission of low priority messages is delayed due to the existence of higher priority messages on the bus. We used Raspberry Pi3 and PiCAN2 board to simulate the data transfer for studying the comparative delay in low priority nodes.

Developmental Rate of Rabbit Parthenogenetic Embryos Derived Using Different Activating Protocols

  • Chrenek, P.;Makarevich, A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.5
    • /
    • pp.617-620
    • /
    • 2004
  • The present study compares development of rabbit embryos generated using different oocyte activation protocols and reconstructed with embryonic or cumulus cells as nuclear donor. In vivo matured oocytes were collected from New Zealand White rabbits at 16 h after ovulation treatment and were activated at18 h of post-ovulation treatment. The following schemes of oocytes activation were tested: 1) single electric pulse (EP, 3.2 kV/cm, 3${\times}$20 $\mu$s, 0.3 M mannitol)+5 min culture in the presence of 5 mM Ionomycin, 2) single electric pulse (EP, 3.2 kV/cm, (${\times}$20 $\mu$s, 0.3 M mannitol)+1 h culture in the presence of 2 mM 6-DMAP, and 3) three electric pulses 30 min apart. Cleavage rate, percentage of expanded and hatched blastocysts as well as total cell number of blastomeres of parthenogenetic embryos were significantly higher using either EP+6-DMAP or 3${\times}$EP schemes, comparing with EP+Ionomycin. Development rate up to hatched blastocyst stage of cloned rabbit embryos using the EP+6-DMAP for activation of nuclei were 19% for embryonic cell nuclei and 36% for cumulus cell nuclei. The best activation protocol optimalized in this study was the combined treatment "P+6-DMAP" which may be potentially used for nuclear transfer protocol.

Area Aware-DSDV Routing Protocol on Ad hoc Networking (Ad Hoc 망에서 AA-DSDV 라우팅 프로토콜)

  • Cho, Se-Hyun;Park, Hea-Sook
    • Proceedings of the Korea Information Processing Society Conference
    • /
    • 2011.04a
    • /
    • pp.590-593
    • /
    • 2011
  • Time goes on, Ad Hoc network is hot issues. So far, there are a lot of protocols have been proposed for Ad Hoc routing protocol to support the mobility. This paper presents an enhanced DSDV(Destination-Sequenced Distance Vector) routing protocol which nominates one node to take care of a specific area. Simply Area-Aware(AA) DSDV routing protocol has one nominee to take care of some area. It has two jobs. One is to take care of its neighbour and another is to transfer the routing table to its other node as it works. It is called as Area Nominee(AN). The new scheme extends the routing table to include the nominee in the area. The general node is the same as the previous DSDV routing protocol. In the other hands, the node which is nominated has two routing protocols. One is for Regional Routing(RR) table which is the same routing table in DSDV. Another is Global Routing(GR) table which is about the area round its area which it cares nearby. GR table is the table for the designated node like the nominee. Each area has one nominee to transfer between ANs. It has only nominee's information about every area. This concept decreases the topology size and makes the information of topology more accurate.

Identification of a Technique Optimized for the Isolation of Spermatogonial Stem Cells from Mouse Testes

  • Han, Na Rae;Park, Hye Jin;Lee, Hyun;Yun, Jung Im;Choi, Kimyung;Lee, Eunsong;Lee, Seung Tae
    • Journal of Embryo Transfer
    • /
    • v.33 no.4
    • /
    • pp.327-336
    • /
    • 2018
  • To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.

A Study on the Performance Analysis of Cache Coherence Protocols in a Multiprocessor System Using HiPi Bus (HiPi 버스를 사용한 멀티프로세서 시스템에서 캐쉬 코히어런스 프로토콜의 성능 평가에 관한 연구)

  • 김영천;강인곤;황승욱;최진규
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.18 no.1
    • /
    • pp.57-68
    • /
    • 1993
  • In this paper, we describe a multiprocessor system using the HiPi bus with pended protocol and multiple cache memories, and evalute the performance of the multiprocessor system in terms of processor utilization for various cache coherence protocols. The HiPi bus is delveloped as the shared bus of TICOM II which is a main computer system to establish a nation-wide computing network in ETRI. The HiPi bus has high data transfer rate, but it doesn't allow cache-to-cache transfer. In order to evaluate the effect of cache-to-cache transfer upon the performance of system and to choose a best-performed protocol for HiPi bus, we simulate as follows: First, we analyze the performance of multiprocessor system with HiPi bus in terms of processor utilizatIOn through simulation. Each of cache coherence protocol is described by state transition diagram, and then the probability of each state is calculated by Markov steady state. The calculated probability of each state is used as input parameters of simulation, and modeling and simulation are implemented and performed by using SLAM II graphic symbols and language. Second, we propose the HiPi bus which supports cache-to-cache transfer, and analyze the performance of multiprocessor system with proposed HiPi bus in terms of processor utilization through simulation. Considered cache coherence protocols for the simulation are Write-through, Write-once, Berkely, Synapse, Illinois, Firefly, and Dragon.

  • PDF

Radio-disjoint Multipath Routing with Dynamic Guard-band Shifting in Wireless Sensor Networks

  • Sangdae Kim;Kyong Hoon Kim;Cheonyong Kim;Tae-Eung Sung;Ki-Il Kim
    • Journal of Internet Technology
    • /
    • v.21 no.4
    • /
    • pp.977-988
    • /
    • 2020
  • A guard-band is used to prevent collision between paths in multipath routing protocols. However, problems such as traffic congestion, reduced lifetime, and void occurrence arise because existing protocols do not exploit the guard-band during packet transmission, but only transfer packets on the same path. To solve these problems, we propose radio-disjoint multipath routing that consists of guard-band shifting and balanced node selection. The proposed scheme transfers a packet on the constructed path and alternatively, into the guard-band area. In addition, the intermediate node is selected according to link quality and remaining energy, so all of the nodes involving packet transmission can be exploited equally. Through a performance evaluation, we demonstrate that the proposed scheme achieves a higher packet delivery ratio and prolongs the average network lifetime by at least 31% and up to approximately 99% compared with existing routing protocols, under the condition of a 90% link success ratio.

A Study of Wireless Sensor Network Routing Protocols for Maintenance Access Hatch Condition Surveillance

  • Lee, Hoo-Rock;Chung, Kyung-Yul;Jhang, Kyoung-Son
    • Journal of Information Processing Systems
    • /
    • v.9 no.2
    • /
    • pp.237-246
    • /
    • 2013
  • Maintenance Access Hatches are used to ensure urban safety and aesthetics while facilitating the management of power lines, telecommunication lines, and gas pipes. Such facilities necessitate affordable and effective surveillance. In this paper, we propose a FiCHS (Fixed Cluster head centralized Hierarchical Static clustering) routing protocol that is suitable for underground maintenance hatches using WSN (Wireless Sensor Network) technology. FiCHS is compared with three other protocols, LEACH, LEACH-C, and a simplified LEACH, based on an ns-2 simulation. FiCHS was observed to exhibit the highest levels of power and data transfer efficiency.