• Transactions of Materials Processing
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• v.15 no.9 s.90
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• pp.692-696
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• 2006

Micro fabrication of polymeric materials becomes increasingly important. And it is considered as a low-cost alternative to the silicon or glass-based Micro Electro-Mechanical System(MEMS) technologies. In the present study, micro channels were fabricated via LiGA(Lithographie, Galvanoformung, Abformung) process used for Capillary Electrophoresis(CE) chip. Taguchi method was applied to investigate the effects of process conditions in injection molding(melt temperature, injection speed, mold temperature and packing pressure) on the transcription characteristics of the micro channel. It was found that the skin layer disturbs a formation of micro channel. Furthermore, mold temperature and injection speed were two important factors to affect the replication characteristics of micro channel.

• MALSORI
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• no.61
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• pp.87-100
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• 2007

A punctuation generation system which combines prosodic information with acoustic and language model information is presented. Experiments have been conducted first for the reference text transcriptions. In these experiments, prosodic information was shown to be more useful than language model information. When these information sources are combined, an F-measure of up to 0.7830 was obtained for adding punctuation to a reference transcription. This method of punctuation generation can also be applied to the 1-best output of a speech recogniser. The 1-best output is first time aligned. Based on the time alignment information, prosodic features are generated. As in the approach applied in the punctuation generation for reference transcriptions, the best sequence of punctuation marks for this 1-best output is found using the prosodic feature model and an language model trained on texts which contain punctuation marks.

• Journal of Microbiology
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• v.35 no.3
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• pp.228-233
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• 1997

The MTF1 gene of Saccharomyces cerevisiae encodes a 43 kDa MITOCHONDRIAL RNA polymerase specificity factor which recognizes mitochondrial promoters to initiate correct transcription. To better understand structure-function of the MTF1 gene as well as the transcription mechanism of mitochondrial RNA polymerase, two cold-sensitive alleles of the MTF1 mutation were isolated by plasmid shuffling method after PCR-based random mutagenesis of the MTF1 gene. The mutation sites were analyzed by nucleotide sequencing. These cs phenotype mtf1 mutants were respiration competent on the nonfermentible glycerol medium at the permissive temperature, but incompetent at 13.deg.C. The cs phenotype allele of the MTF1, yJH147, encoded an L146P replacement. The other cs allele, yJH148, contained K179E and K214M double replacements. Mutations in both alleles were in a region of Mtflp which is located between domains with amino acid sequence similarities to conserved regions 2 and 3 of bacterial s factors.

• Korean Journal of Veterinary Research
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• v.57 no.1
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• pp.37-42
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• 2017

Getah virus (GETV) infection causes sporadic outbreaks of mild febrile illness in horses and reproductive failure in pigs. In this study, we established a reverse transcription polymerase chain reaction (RT-PCR) method to detect GETV from suspected virus-infected samples. The reaction conditions were optimized and validated by using RNA extracted from GETV propagated in cell culture. A GETV-specific GED4 primer set was designed and used to amplify a 177 bp DNA fragment from a highly conserved region of the E1 glycoprotein gene in the GETV genome. RT-PCR performed with this primer set revealed high sensitivity and specificity. In the sensitivity test, the GED4 primer set detected GETV RNA at the level of $10^{2.0}\;TCID_{50}/mL$. In the specificity test, the GED4 primer set amplified only a single band of PCR product on the GETV RNA template, without non-specific amplification, and exhibited no cross-reactivity with other viral RNAs. These results suggest that this newly established RT-PCR method is useful for accurate identification of GETV infection in animals.

• Proceedings of the Botanical Society of Korea Conference
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• 1998.07a
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• pp.115.2-116
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• 1998

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.04a
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• pp.55-56
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• 2009

A light guiding plate (LGP) with nanometer-sized patterns was fabricated by injection molding method which employed electrically healed mold and the transcription of injection-molded parts was investigated. A Ni stamper was fabricated using MEMS technology. The Ni stamper was then installed in a movable heated core which is a key part of the mold. Using this mold, injection-molded plastic LGP parts were manufactured at different mold temperatures and the effect of the temperature on the transcription of the parts was investigated.

• Transactions of Materials Processing
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• v.16 no.1 s.91
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• pp.9-14
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• 2007

In this paper, a mold temperature control method for injection molding is proposed. The inner surface of mold is locally heated by jet impingement to improve pattern transcription. Heating by hot jet is completed while the mold is open. An experimental system that realizes the proposed idea has been built, which includes mold, nozzle assembly and heater. Actual injection molding process including the proposed heating procedure has been conducted to verify the validity of the method. The process has been done for several conditions with different jet temperatures and duration of heating. The results from different conditions are compared.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1398-1403
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• 2016

The simultaneous detection and accurate identification of hepatitis A virus (HAV) is critical in food safety and epidemiological studies to prevent the spread of HAV outbreaks. Towards this goal, a one-step duplex reverse-transcription (RT)-PCR method was developed targeting the VP1/P2B and VP3/VP1 regions of the HAV genome for the qualitative detection of HAV. An HAV RT-qPCR standard curve was produced for the quantification of HAV RNA. The detection limit of the duplex RT-PCR method was 2.8 × 10¹ copies of HAV. The PCR products enabled HAV genotyping analysis through DNA sequencing, which can be applied for epidemiological investigations. The ability of this duplex RT-PCR method to detect HAV was evaluated with HAV-spiked samples of fresh lettuce, frozen strawberries, and oysters. The limit of detection of the one-step duplex RT-PCR for each food model was 9.4 × 10² copies/20 g fresh lettuce, 9.7 × 10³ copies/20 g frozen strawberries, and 4.1 × 10³ copies/1.5 g oysters. Use of a one-step duplex RT-PCR method has advantages such as shorter time, decreased cost, and decreased labor owing to the single amplification reaction instead of four amplifications necessary for nested RT-PCR.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.5 no.3
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• pp.218-222
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• 2004

We developed the stamper structured mold with moving core type with nano pattern. Among the factors affecting the quality of injection molding plastic parts, We studied the effects of moving core surface heating method on the transcription of injection molding plastic parts with nano structures. Moving core surface heating has been tested by three different methods. The first was conventional injection molding process without heating moving core surface, the second was halogen lamp radiation heating process and the last was MmSH process using gas flame. As a result of making injection molded parts by using thermoplastic amorphous resins such as COC, PMMA, MmSH method which is the most high temperature of moving core surface showed the best nano pattern transcription of the three methods, but the outcome of conventional injection molding process was not better than others.

• Journal of architectural history
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• v.16 no.1
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• pp.29-48
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• 2007

This study is an investigation that explication of the reading method of transcription marking, and explains what it means and where to use transcription marking of architectural vocabulary. focusing on timber class vocabulary from on SeoGwolYeongGeonDoGamUiGwe(${\ulcorner}$西闕營建都監儀軌(1831)${\lrcorner}$). This study is reviewed sequentially classifying items according to timber class, stone class, iron class architectural vocabulary. This treatise, named 'Architectural Vocabulary Study (1)', is mainly focused on making out a transcription marking of timber class vocabulary on the documents. But, because of the properties of the UiGwe(儀軌), there are some different expressions used for the same vocabulary. Therefore, the different expressions are referenced according to vocabulary used from the 17th century to the 20c century. This study lists timber class architectural vocabulary of transcription marking on SeoGwolYeongGeonDoGamUiGwe(${\ulcorner}$西闕營建都監儀軌(1831)$\lrcorner$). This study also explicates and explains the meaning of them. In advance, this study compares them with the vocabulary of national language in the middle ages and modern times. If we could explicate the transcription marking of the documents as it is, we can't only recover plenty of vocabulary related with characteristic architecture in the age of later Chosun Dynasty, but also correct wrongly used vocabulary nowadays. With the results, we can standardize and adjust vocabulary use of Korean traditional architecture. In advance, we can correct errors of spelling and mistaken explanation in the Korean Encyclopedia.