• 한국콘텐츠학회논문지
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• 제12권2호
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• pp.339-348
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• 2012

급성 전골수구성 백혈병(acute promyelocytic leukemia, APL)은 치료제가 한정적이고 그 또한 다양한 부작용을 초래한다. 최근 암세포 형성 억제에 세균 추출물을 사용하는 경우가 증가하는데 이를 이용하여 기존의 약제보다 효과적이면서 부작용이 적은 치료제 개발이 필요하다. 본 연구에서는 L. monocytogenes에서 분비되는 물질(LmSup)과 세균 자체가 함유하고 있는 물질(LmE)을 추출하여 HL-60 세포에 처리한 다음 세포증식 억제 효과를 보고자 하였다. 세포 생존율 및 세포고사를 확인하여 세포를 죽음으로 유도하는 지 파악한 다음 작용기전을 규명하고자 세포주기의 변화 및 ROS 생성을 관찰하였다. 그 결과, LmSup와 LmE가 급성 전골수구성 백혈병(APL) 세포인 HL-60의 세포고사를 유도하고, sub G0/G1기 증가로 세포주기를 비정상적으로 차단함으로써 세포고사를 유도함을 확인하였다. 이때, ROS가 관여함을 관찰하였다. 이를 통해, LmSup 또는 LmE의 구체적인 항암효과 및 기전 분석을 통해 난치병인 APL의 치료 방법 및 치료제 개발에 기여하고자 한다.

• 트랜스-
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• 제11권
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• pp.119-142
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• 2021

영상은 이미지를 전제로 하는 문화적 실천이다. 영상이미지를 통해 관객은 일상이 아닌 새로운 것을 체험하기를 갈망한다. 영상이미지의 구성 요소는 많지만 그중에 시각적 표현인 색채가 큰 역할을 담당하고 있다. 컬러영화가 등장하기 시작하면서부터 색채는 영상예술의 중요한 구성요소로서 끊임없이 발전하고 혁신적인 영상미술 디자인에서 중요한 역할을 하였다. 영화인들은 1895년 영화가 흑백으로 탄생할 때부터 색채에 대한 관심이 있었다. 이 두 가지 색채로 더 이상 시청자를 만족시키지 못했기 때문에 필름에 색채를 입히는 등 더 많은 색채를 영화 속에서 표현하려고 노력했다. 영상에서의 색채는 표현의 기법이면서도 정신과 사상으로 이해할 수 있다. 색채가 단순히 그냥 존재하는 것이 아니라 인간심리에 강하게 작용하고 있음에 일러주는 것이기도 하다. 색채는 영화 언어의 일부로, 색채언어는 영화 속에서 영화의 사상적 주제를 표현하거나 선명한 캐릭터를 묘사할 수 있으며, 사람들에게 더 직관적으로 메시지를 전달할 수 있다. 본 연구는 색 조명 방식 혹은 배색 방안인 바이섹슈얼 라이팅을 대상으로, 그의 기본적인 색채 구성요소, 즉 핑크색, 파란색 및 보라색이 갖고 있는 색채 특성을 이해해 색채를 통해 인간심리가 어떤 영향을 받고 있는 지에 대해 분석하고 바이섹슈얼 라이팅이 영상에서 나오는 장면들을 결합해 구체적인 사례분석을 했다. 이를 통해 바이섹슈얼 라이팅이 영상에서 색채 속성을 이용해 표현한 색채 언어가 무엇이고 바이섹슈얼 라이팅이 어떻게 색채를 통해 인간심리와 상호 작용하는지를 탐색하였다.

• 로봇학회논문지
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• 제3권4호
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• pp.345-350
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• 2008

This study aims to propose the concept design of oil spill protection robot which can rapidly intervene to control the oil spillage situation at the sea. Taking into account the fact that a huge amount of oil is transported trans-continentally by oil tanker, none of industrialized countries are completely safe from the marine oil spill which results in social, economical and ecological damages to their communities. The employment of double hull-oil tanker, pipe line transporting can be most safe way. Yet complete prevention of oil spill is probably not realistic. Accordingly the alternative solution to control marine oil spill and minimize the damages caused by the incident using intelligent robot technology based on swarm control method is proposed. The main features of oil spill protection(OSP) robot is explained via following three perspectives. Firstly, from functional point of view, OSP robot system safely and efficiently replaces oil boom installation manually conducted by human workers with intelligent robot technology based on swarm control theory. For second, its modular architecture brings efficient storage of main components including oil boom and facilitates maintenance. For the last, its geometric form and shape enables whole system to be installed to helicopter, boat or oil tanker itself with ease and to rapidly deploy the units to the oil spill area.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6803-6812
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• 2015

Caveolin-1 is a 22-kD trans-membrane protein enriched in particular plasma membrane invaginations known as caveolae. Cav-1 expression is often dysregulated in human breast cancers, being commonly upregulated in cancer cells and downregulated in stromal cells. As an intracellular scaffolding protein, Cav-1, is involved in several vital biological regulations including endocytosis, transcytosis, vesicular transport, and signaling pathways. Several pathways are modulated by Cav-1 including estrogen receptor, EGFR, Her2/neu, $TGF{\beta}$, and mTOR and represent as major drivers in mammary carcinogenesis. Expression and role of Cav-1 in breast carcinogenesis is highly variable depending on the stage of tumor development as well as context of the cell. However, recent data have shown that downregulation of Cav-1 expression in stromal breast tumors is associated with frequent relapse, resistance to therapy, and poor outcome. Modification of Cav-1 expression for translational cancer therapy is particularly challenging since numerous signaling pathways might be affected. This review focuses on present understanding of Cav-1 in breast carcinogenesis and its potential role as a new biomarker for predicting therapeutic response and prognosis as well as new target for therapeutic manipulation.

• 대한의생명과학회지
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• 제10권3호
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• pp.187-194
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• 2004

A short peptide corresponding to the nuclear localization signal (NLS) of human immunodeficiency virus (HIV)-l Tat protein, Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg, was employed to improve the efficiency of cellular uptake of nucleic acids. The peptide was first mixed with a reporter plasmid and then with cationic liposomes to form a tripartite complex of DNA/peptide/liposomes (DPL). Transfection efficiency of the DPL complex was compared with that of the conventional DNA/liposomes (DL) complex. When the DPL complex was formed with various cationic liposomes, DOTAP/DOPE (DP) liposome exhibited superior transfection efficiency to other liposomes tested in vitro. With the inclusion of the peptide, the DPL complex showed much enhanced transfection in various cancer cell lines. Particularly, transfection of the DPL complex in serum increased cellular uptake of a transgene up to 2 fold when compared with that in a serum free condition. Further, when the DPL complex was infused through the ureteric route of a rat, transfection efficiency was shown to be better in reporter gene expression than that obtained with the DL complex. This study shows that the DPL complex that is easy to formulate can be employed for much enhanced cellular uptake of a trans gene.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.102-102
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• 2003

The inhibitors of prostaglandin biosynthesis and nitric oxide production by corresponding inducible isozyme have been considered as potential anti-inflammatory and cancer chemopreventive agents. In our continuous search for cancer chemopreventive agents from natural products, we have evaluated the inhibitory potential of PGE$_2$and NO production in lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 cells. As a result, pinosylvin (3,5-dihydroxy-trans-stilbene), a stilbenoid, mainly found from the heartwood and leaves of the Pinus sylvestris, showed potential inhibitory activity of LPS-induced PGE$_2$and NO production in a dose-dependent manner. Pinosylvin also suppressed the LPS-induced iNOS protein expression. Further study revealed that pinosylvin exhibited antioxidant activity by the DPPH free radical scavenging potential and inhibitory effect of xanthine oxidase activity. In addition, pinosylvin inhibited COX -2 overexpressed human colon cancer cell (HT-29) growth in a time- and dose-dependent manner. These findings suggest that pinosylvin might be a promising candidate for developing cancer chemopreventive agent.

• 약학회지
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• 제38권3호
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• pp.230-237
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• 1994

The structure-activity relationships of (E)-chalcone-4-carboxylic acids, flavone-4'-carboxylic acids, two types of aromatic amides, terephthalic monoanilides, and (arylcarboxamido)benzoic acids, which were made by Shudo group, are discussed by conformation analysis(AM1) of retinoic acid and those compounds. Conformer of each compound is superimposed on the conformationally restricted compound, 4-(6,7,8,9-tetrahydro-6,6,9,9-tetramethyl-4H-4-oxonaphto[ 2,3-b]pyran-2-yl) benzoic acid(Fv80), possessing the strongest differentiation-inducing activity on human promyelocytic leukemia cells HL-60. The results indicated that the lengths between the carboxylic carbon and the two 6, 9 carbons binding to dimethyl, 1.20 nm and 1.09 nm, as well as the planarity of molecule are very important factors for the activity, especially 1.20 nm. In the case of the recently synthesized azulenic retinoic acids by Sato, et al. in 1993, the distance probably is also important, resulted from superimposing them on a Ch55 conformer and Fv80. The distance 1.0 nm is also important in Ch55. Several conformers of all-trans retinoic acid (RA) are well superimposed on the almost non-flexible Fv80, RA, 9-cis RA, and, specifically s-10,12 cis RA. And a simple hexangular model of RA is suggested to draw RA conformers easily without computer drawing model or molecular model.

• 여성건강간호학회지
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• 제16권4호
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• pp.317-325
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• 2010

Purpose: This study was to examine the differences in health belief variables and HPV (Human Papillomavirus) knowledge in Pap smears according to the stages of change using Trans-theoretical Model (TTM) among married nurses. Methods: The research design was a cross sectional survey. Participants were 387 married nurses working in general hospital located metropolitan city. Measurement variables were perceived threatening, perceived severity, benefits of Pap, HPV knowledge and stages of the change in Pap smear. Results: Classified stages were pre-contemplation 3.9%, contemplation 17.8%, relapsing 29.5%, action 31.0%, and maintenance 17.8%. Accuracy rate of HPV knowledge were low ranging from 15% to 45%. Among the variables, perceived threatening (F=3.56, p=.007), perceived severity (F=9.73, p<.001) and HPV knowledge (F=7.52, p<.001) were significantly different by stages of change. Conclusion: Application of TTM to Pap smears was efficient to know the nurse's level of health behaviors, Health belief variables and HPV knowledge were main factors to identify the stages of change. The continuation of Pap smears and the education in relation to HPV knowledge should be encouraged for married nurses.

• 한국실내디자인학회논문집
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• 제17권5호
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• pp.31-39
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• 2008

Each human era has its own unique architectural embodiment of religious philosophy at the time. Though, many modern religions have developed their own architectural expression based upon each own religious doctrine, one of the main native Korean religions, Won-Buddhism, has not developed its own proper architectural expression yet. So, the goal of this research is to find the probable architectural design methods for Won-Buddhism doctrines. 1) The history of Won-Buddhism architecture is comparatively analyzed to find the architectural trend of Won-Buddhism. 2) Modern religious architectural cases are analysed and compared between plastic expression and spatial expression types. 3) Won-Buddhism doctrines are analysed to find key characteristics to be used as architectural design concepts and method. Based upon analyses, the main characteristics of Won-Buddhism doctrine are synchronicity, circularity, and liminality, which are found to be similar to the trans-boundary characteristics of contemporary architecture. In the future, more study on these characteristics and how they are materialized, the probable design methodology for Won-Buddhism Doctrines can be proposed.

• Parasites, Hosts and Diseases
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• 제52권1호
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• pp.117-120
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• 2014

Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to citrus-cultivating farmers. A cysteine protease was purified using column chromatography of Mono Q anion exchanger and Superdex 200 HR gel filtration. It was estimated to be 46 kDa by gel filtration column chromatography and consisted of 2 polypeptides, at least. Cysteine protease inhibitors, such as trans poxy-succinyl-L-leucyl-amido (4-guanidino) butane (E-64) and iodoacetic acid (IAA) totally inhibited the enzyme activities, whereas serine or metalloprotease inhibitors did not affect the activities. In addition, the purified enzyme degraded human IgG, collagen, and fibronectin, but not egg albumin. From these results, the cysteine protease of the mites might be involved in the pathogenesis such as tissue destruction and penetration instead of nutrient digestion.