• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.5
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• pp.35-41
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• 2008

The purpose of this study is to determine those factors critical in the determining of regional trail routes. Initially, a consideration of the concept of the regional trail was conducted through literature reviews and 13 planning elements were determined from previous studies related to trail planning and trail construction studies. An additional 13 items were taken from literature research related to nature experiences or were sourced by interviewing experts. To verify these planning elements, a survey of experts was conducted. Individual elements were assessed concerning their degree of importance. As a result of this survey, it was found that the protection of ecologically sensitive areas and the use of existing trail routes are important route planning factors. These planning factors can be used for making decisions regarding regional trail route directions and situations.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1201-1207
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• 2011

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been proposed as a potent tool to trigger apoptosis in cancer therapy. However, as many types of cancer cells remain resistant towards TRAIL-induced cytotoxicity, several combined therapy approaches aimed to sensitize cells to TRAIL have been developed. Genistein, a natural isoflavonoid phytoestrogen, has been shown to have anticancer activity by inducing cell cycle arrest at G2M phase as well as apoptosis in various cancer cell lines. In the present study, we showed that treatment with TRAIL in combination with subtoxic concentrations of genistein sensitized U937 human leukemia cells to TRAIL-mediated apoptosis. Combined treatment with genistein and TRAIL effectively activated caspases through Bid truncation (tBid) and down-regulation of cellular caspase-8 (FLICE)-like inhibitory proteinL ($cFLIP_L$). However, the apoptotic effects of co-treatment with genistein and TRAIL were significantly inhibited by specific caspase inhibitors, which demonstrates the important role of caspases in apoptosis induced by genistein and TRAIL. Overall, our results indicate that genistein can potentiate TRAIL-induced apoptosis through down-regulation of $cFLIP_L$ and up-regulation of pro-apoptotic tBid proteins.

• Journal of Life Science
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• v.22 no.10
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• pp.1277-1285
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• 2012

The tumor necrosis, factor-related, apoptosis-inducing ligand (TRAIL) is regarded as a potentially useful anticancer agent with excellent selectivity for cancer cells. However, a considerable number of cancer cells are resistant to apoptosis induction by TRAIL. Developing strategies to overcome this resistance are important for the successful use of TRAIL for cancer therapy. Here, we revealed that siRNA-mediated downregulation of SIRT1 or SIRT1 inhibitor Amurensin G upregulated DR5 and c-Myc and downregulated c-$FLIP_{L/S}$ and Mcl-1, which was associated with sensitization of TRAIL-resistant MCF-7 cells to TRAIL. This result was followed by the activation of caspases, PARP cleavage, and downregulation of Bcl-2 in both TRAIL-treated MCF-7 cells transfected with SIRT1 siRNA and cells co-treated with Amurensin G and TRAIL. Our results suggest that the induction of DR5 and downregulation of c-FLIP via suppression of SIRT1 expression may be a useful strategy to increase the susceptibility of TRAIL-resistant cancer cells to TRAIL-induced cell death.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10697-10703
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• 2015

The laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors occurring in the head and neck. Tumor necrosis factor related apoptosis induce ligand (TRAIL) and TRAIL-receptors (DR4, DR5, DcR1, DcR2) are known as important members of TRAIL-mediated biochemical signaling pathway. Associations between polymorphisms in these genes and clinicopathological characteristics of human laryngeal carcinoma are not well defined. This study therefore aimed to investigate a possible relationship among the TRAIL and TRAIL-DR4 polymorphisms and sTRAIL levels in the risk or progression of LSCC. A total of 99 patients with laryngeal cancer and 120 healthy subjects were enrolled in the study. DR4 C626G and TRAIL 1595 C/T genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and sTRAIL levels were measured by ELISA. There were significant differences in the distribution of DR4 C626G genotypes and frequencies of the alleles between laryngeal cancer patients and controls (p<0.001) but not in TRAIL 1595 C/T. We found the increased frequency of the DR4 C626G homozygote CC genotype in patients than in controls (p<0.001). Haplotype analysis revealed that there was also a statistically significant relationship between TRAIL and TRAIL-DR4 polymorphisms and laryngeal cancer. Serum sTRAIL levels in the laryngeal patients with CC genotype who had advanced tumour stage were lower than those of patients with early tumor stage (p=0.014). Our findings suggest that DR4 C626G genotypes and sTRAIL levels might be associated with progression of laryngeal cancer in the Turkish population.

• Korean Journal of Environment and Ecology
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• v.9 no.2
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• pp.211-220
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• 1996

5 major trails of Odaesan National Park were selected to investigate the use impacts on environmental deterioration of trail accreding to the different amunt of use. The entire width, bare width, maximum depth and slope of trail as the trail comdition surveyed at the total of 120 points were significantly greater on the more heavily-used trail. Major deterioration types of trail were root-exposure, rock-exposure, deepening and divergence in order of frquency and trail conditions of deteriorated points were significantly different from those of non-deteriorated points. The damaged area more severe than Class 4 on trail reached about 379m$^{2}$/km in total, and especcially about 1,196m$^{2}$/km in the section between Chodmyolbogung and Pirobong of Sangwonsa trail. The dominant trees of trail edge vegetation could be Quercus mongolica in upper layer, but in lower layer Rhododecdron schilipenbachii for slope and Actinidia arguta for ridge. Coverage and number of individuals of lower vegetation in trail edge were the lowest on the Sangwonsa trail of the largest use amount, and each trail was dissimilar in species composition of lower layer of edge vegetation.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8041-8046
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• 2014

In-depth analysis of how TRAIL signals through death receptors to induce apoptosis in cancer cells using high throughput technologies has added new layers of knowledge. However, the wealth of information has also highlighted the fact that TRAIL induced apoptosis may be impaired as evidenced by experimental findings obtained from TRAIL resistant cancer cell lines. Overwhelmingly, increasing understanding of TRAIL mediated apoptosis has helped in identifying synthetic and natural compounds which can restore TRAIL induced apoptosis via functionalization of either extrinsic or intrinsic pathways. Increasingly it is being realized that biologically active phytochemicals modulate TRAIL induced apoptosis, as evidenced by cell-based studies. In this review we have attempted to provide an overview of how different phytonutrients have shown efficacy in restoring apoptosis in TRAIL resistant cancer cells. We partition this review into how the TRAIL mediated signaling landscape has broadened over the years and how TRAIL induced signaling machinery crosstalks with autophagic protein networks. Subsequently, we provide a generalized view of considerable biological activity of coumarins against a wide range of cancer cell lines and how coumarins (psoralidin and esculetin) isolated from natural sources have improved TRAIL induced apoptosis in resistant cancer cells. We summarize recent updates on piperlongumine, phenethyl isothiocyanate and luteolin induced activation of TRAIL mediated apoptosis. The data obtained from pre-clinical studies will be helpful in translation of information from benchtop to the bedside.

• Korean Journal of Environment and Ecology
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• v.18 no.4
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• pp.437-445
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• 2004

The purpose of this study was to provide basic visitor and trail use data for developing trail management strategies in Korean national park system. To accomplish the purpose of the study, a questionnaire survey was carried on at two different national parks(Bukhansan and Chiaksan National Park). As for the results, root and rock exposure on the trails and trail width expansion were perceived as major types of trail impact. In addition, majority of respondents pointed to construct or maintain facilities on steep paths and repair trail surface as for the priority of trail maintenance. In conclusion, the priority of trail management and maintenance has to be differently determined based on each trail conditions because visitor impacts on the trails vary along with site-specific and activity-specific differences. Findings of the study expect to be used as fundamentals in trail management strategy making for Korean national parks.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.469-479
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• 2012

Trail is a main infrastructure for various forest recreational activities. In this study, the recreational supply (trail) and demand (trail program according to visitor characteristics, preferred forest activities) were studied. Through the application of a multiple correspondence analysis and cross tabulation analysis, 3 trails (Bukhansan trail, Uljin trail, Jirisan trail) were identified. On this study, visitor demands for trail program participation, preferred forest recreational activities, program contents and program operations. Visitors expressed positive attitude toward trail program participating and showed characteristics with the differences. Young visitors preferred active experience program and middle aged visitors more liked health program such as forest therapy. On site program and self guided interpretation were preferred for program operation. In Bukhansan trail, continuous health program (1~2 hours) would be proper and it needs to adapt various nature activities in the Uljin trail. For the Jirisan trail, self guided program would be developed in many ways.

• Herbal Formula Science
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• v.25 no.2
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• pp.145-154
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• 2017

Objectives : Bufalin is one of the bioactive component of 'Sum Su (蟾酥)', which is obtained from the skin and parotid venom gland of toad. Bufalin has been known to possess the inhibitory effects on cell proliferation and inducing apoptosis in various cancer cells. The tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) has concerned, because it can selectively induce apoptotic cell death in many types of malignant cells, while it is relatively non-toxic to normal cells. Here, we investigated whether bufalin can trigger TRAIL-induced apoptotic cell death in EJ human bladder cancer cells. Methods : Effects on the cell viability and apoptotic activity were quantified using MTT assay and flow cytometry analysis, respectively. To investigate the morphological change of nucleus, DAPI staining was performed. Protein expressions were measured by immunoblotting. Results : A combined treatment with bufalin (10 nM) and TRAIL (50 ng/ml) significantly promoted TRAIL-mediated growth inhibition and apoptosis in EJ cells. The apoptotic effects were associated with the up-regulation of death receptor proteins, and the down-regulation of cFLIP and XIAP. Moreover, our data showed that bufalin and TRAIL combination activated caspases and subsequently increased degradation of poly(ADP-ribose) polymerase. Conclusions : Taken altogether, the nontoxic doses of bufalin sensitized TRAIL-mediated apoptosis in EJ cells. Therefore, bufalin might be an effective therapeutic strategy for the safe treatment of TRAIL-resistant bladder cancers.

• YAKHAK HOEJI
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• v.55 no.1
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• pp.49-55
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• 2011

Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is one of the promising anti-cancer agent because of its ability to selectively induce apoptosis in tumor cell lines but not in normal cells. However, TRAIL resistance has been reported in some cancer cells including hepatocarcinoma cells. Therefore, studies of agents that sensitize TRAIL-resistant cancer cells could be a effective therapeutic approach in cancer management. In our study, we examined the effect of combination of TRAIL with apigenin in human hepatocellular carcinoma cells. As a result, the combined use of TRAIL and apigenin significantly enhanced the cytotoxicity in PLC-PRF5 cells. Flow cytometry analysis after annexin V-FITC/PI dual staining showed that this increase of cell cytotoxicity was related to enhanced apoptosis in combined treatment of TRAIL with apigenin. Furthermore, synergistic induction of apoptosis was also confirmed by observation of morphological changes and annexin V-FITC/PI fluorescence. Our findings suggests that apigenin has the potential to improve the efficiency of TRAIL-based therapies in human hepatocellular carcinoma cells. Further study is needed to reveal the molecular mechanisms of this combined therapy.