• Journal of Ginseng Research
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• v.43 no.3
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• pp.377-384
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• 2019

Background: Inflammation is widespread in the clinical pathology and closely associated to the progress of many diseases. Triterpenoid saponins as a key group of active ingredients in Panax notoginseng (Burk.) F.H. Chen were demonstrated to show antiinflammatory effects. However, the chemical structures of saponins in the leaves and stems of Panax notoginseng (PNLS) are still not fully clear. Herein, the isolation, purification and further evaluation of the antiinflammatory activity of dammarane-type triterpenoid saponins from PNLS were conducted. Methods: Silica gel and reversed-phase C8 column chromatography were used. Furthermore, preparative HPLC was used as a final purification technique to obtain minor saponins with high purities. MS, NMR experiments, and chemical methods were used in the structural identifications. The antiinflammatory activities of the isolated saponins were assessed by measuring the nitric oxide production in RAW 264.7 cells stimulated by lipopolysaccharides. Real-time reverse transcription polymerase chain reaction was used to measure the gene expressions of inflammation-related gene. Results: Eight new minor dammarane-type triterpene oligoglycosides, namely notoginsenosides LK1-LK8 (1-8) were obtained from PNLS, along with seven known ones. Among the isolated saponins, gypenoside IX significantly suppressed the nitric oxide production and inflammatory cytokines including tumor necrosis $factor-{\alpha}$, interleukin 10, interferon-inducible protein 10 and $interleukin-1{\beta}$. Conclusion: The eight saponins may enrich and expand the chemical library of saponins in Panax genus. Moreover, it is reported for the first time that gypenoside IX showed moderate antiinflammatory activity.

• Analytical Science and Technology
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• v.29 no.1
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• pp.1-9
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• 2016

It is believed that traditional Korean medicines can be managed more scientifically through the development of logical criteria to verify their region of cultivation, and that this could contribute to the advancement of the traditional herbal medicine industry. This study attempted to determine such criteria for Sansuyu. The volatile compounds were obtained from 20 samples of domestic Corni fructus (Sansuyu) and 45 samples of Chinese Sansuyu by steam distillation. The metabolites were identified in the NIST Mass Spectral Library via the obtained gas chromatography/mass spectrometer (GC/MS) data of 53 training samples. Data binning at 0.2 min intervals was performed to normalize the number of variables used in the statistical analysis. Multivariate statistical analyses, such as principle component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) were performed using the SIMCA-P software package. Significant variables with a variable importance in the projection (VIP) score higher than 1.0 were obtained from OPLS-DA, and variables that resulted in a p-value of less than 0.05 through one-way ANOVA were selected to verify the marker compounds. Finally, among the 11 variables extracted, 1-ethylbutyl-hydroperoxide (9.089 min), nonadecane (20.170 min), butylated hydroxytoluene (25.319 min), 5β,7βH,10α-eudesm-11-en-1α-ol (25.921 min), 7,9-bis(2-methyl-2-propanyl)-1-oxaspiro[4.5]deca-6,9-diene-2,8-dione (34.257 min), and 2-decyldodecyl-benzene (54.717 min) were selected as markers to indicate the origin of Sansuyu. The statistical model developed was suitable for the determination of the geographical origin of Sansuyu. The cultivation areas of four Korean and eight Chinese Sansuyu samples were predicted via the established OPLS-DA model, and it was confirmed that 11 of the 12 samples were accurately classified.

• Journal of the Korean Society for Library and Information Science
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• v.51 no.4
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• pp.35-52
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• 2017

This paper aims to explore the citation patterns of conference papers in 'Information Science' discipline and to analze impacts of various cited works-related factors on future citations of conference papers. Existing bibliometric studies has investigated citation patterns and the statistical relations between a variety of bibliographic factors and the future citations of literature. However, the attentions have been focused largely on journal articles, and the bibliometric studies targeting conference papers are still in an infant stage. Therefore, this study, which is based on 1,904 conference papers in 'Information Science' field, examined several citation patterns and the contributions of the factors about cited works - the number of cited works, type of cited works, citation rates and ages of cited works at the time of being cited and the rate of self-citedness - to the future citation of the citing target articles. The data source of this study including the properties of target articles and cited works and citation rates of target articles was Scopus. As the results, 53% and 29% of the cited works were conference papers and journal articles, respectively. 14% of them are non-traditional types such as web pages, technical reports, patent, etc. More than 60% of the cited works were 5 years old or less. Among several factors considered in this paper, the number of conference papers and the number of non-traditional types of works are the most contributing factors on the citation rates of target articles. The recency of the cited works is also significant contributor on the citation rates of target articles. That is, the target articles citing more conference papers and non-traditonal types of works earned more citations. The target articles citing recent works also earned more citations.

• Journal of the Korean Society for Library and Information Science
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• v.21
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• pp.61-101
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• 1991

This study reviews the development and change of main entry principle in German cataloging codes, with special emphasis on RAK. With rerard to the functions of catalog, comparison between the traditional title main entry in the Orient and author main entry in the West has been made. The analysis confirms in this study that various criteria in choice of the entries in RAK have been adopted. In case of works where the persons who have played different roles in the works are named on the title page, as well as related works and works of mixed responsibility, the criteria of entry determination are complex and time consuming process and have no absolute value. And there are also various kinds of problems in corporate entries including confirmation of originator(Urheber), choice of either the territorial authority corncerned or corporate bodies as an entry depending on the nature of the publications, and a unique bibliographical situation of treaties. This means the code is absence of absolute value in selecting entries, and this results in adoption of main entry principle which has lost its significance for the purpose of cataloging. With emergence of the ISBD and actualization of automated cataloging, morever, all entries are equal as points of access. It would eliminate the need for personal judgements required in choice of main entry by the present code. In doing so, it would bring uniformity and standardization to cataloging practice. In direct approach to works, title entry is more developed finding device than author entry in cataloging theories. Thus introduction of unit card system beginning with title which is adopted in KCR3 would be desirable, the complicated rules for the choice of entry could be abandoned from cataloging codes. Most of the user studies show that catalog users have placed higher value on the title entry as a finding device and each entry is equal as access points through unit entry. This means that choice of a given entry as a main entry is unnecessary in cataloging codes. Title entry would be a rather simple standard and direct approach for works. This study proves that the traditional title entry of Korea is superior to author main entry in the Western world in cataloging theory. Thus recommendation to be made is that abandonment of author main entry from cataloging codes should be considered in the future.

• Journal of Ginseng Research
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• v.47 no.5
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• Journal of the Korean Society for Library and Information Science
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• v.44 no.2
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• pp.117-137
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• 2010

The purposes of this study are to review the historical development of food and culture in the KDC (Korean Decimal Classification) and to propose improvements of the KDC to classify materials of food and culture by using the KDC effectively. First of all, changes of classification numbers and headings related to food and culture from the 1st edition to the 5th edition of the KDC were examined. Recent books about food and culture were examined and were classified according to the latest edition of the KDC. Several problems were found including a lack of headings, including notes in food and culture, a lack of headings about Korean foods in particular and reflections about various foods from other countries, a lack of detailed relative headings, and the remaining western oriented headings. Other classification systems about food and culture were analyzed and it was found that there was a need to have new headings for classifying Korean traditional foods and table services, new formats for a relative index, detailed notes, and changes in western oriented headings.

• Journal of the Korean Society for Library and Information Science
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• v.44 no.1
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• pp.101-115
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• 2010

The main difference between web searching and traditional searching is that the web provides and supports multimedia searching. This study aims to investigate the multimedia searching behavior of users of NAVER, a major Korean search portal. In conducting this study, the query logs and click logs of a unified search service were analyzed. The results of this study show that among the multimedia queries submitted by users, audio searches are the dominant media type, followed similarly by video and image searches. On the other hand, among the multimedia documents clicked on, video is the most popular collection type followed by image and audio collections. Entertainment is the most popular topic in both multimedia queries and clicks. The results of this study can be implemented for the portal's development of multimedia content and searching algorithms.

• Journal of Korean Library and Information Science Society
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• v.42 no.4
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• pp.31-50
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• 2011

In education, evaluation is an important element because instructor can judge the growth and development of students, it has been to bring about quality improvement in education. Performance assessment has emerged to overcome traditional evaluation such as paper test, multiple choice test. Performance assessment is an approach to measuring a student's status based on the way the student completes a specified task. Information literacy instruction is aimed at cultivating information literacy. Performance assessment suitable for measurement to status of Information literacy. In order to performance assessment, task should have the first. However, instructor is difficult to develop a good performance task to meet the assessment needs. Therefore, this research aims at suggesting the developmental tool of performance task applicable to Information literacy instruction. The proposed tool is a template type, consists of 4 steps, educational goals and learning objectives, knowledge and capabilities, task types, measurement and scoring criteria.

• The Journal of Internal Korean Medicine
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• v.39 no.3
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• pp.389-404
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• 2018

Objective: The aim of this study was to investigate acupuncture therapy for post-stroke cognitive disorder to suggest the methods of a study about acupuncture therapy. Methods: Several academic databases were used in this study, including National Digital Science Library (NDSL), National Assembly Library of Korea, Korean Traditional Knowledge Portal, Research Information Sharing Service (RISS), Korean studies Information Service System (KISS), Korean Medical Database (KMBASE), Korea Med, Oriental Medicine Advanced Searching Integrated System (OASIS), PubMed, MEDLINE with Full Text, and China National Knowledge Infrastructure (CNKI). Keywords used were "중풍", "뇌졸중", "인지장애", "침", "acupuncture", and "cognitive disorder". Results: Ultimately, 28 papers were investigated. that were mainly published in 2012 and 2016. Of these, More than two-thirds received a score of one or less on the Jadad scale. Acupuncture points such as GV20, GV24, PC6, EX-HN1, SP6, and GV26 were most frequently used. The treatment duration was mostly 30 minutes or less, and the treatment cycle was mostly for four weeks. Dilatational wave was generally used in pulse wave form, and amplitude of electricity was increased until patients were able to endure. G6805 was generally used in electric acupuncture apparatus. According to results of acupuncture treatment, assessments such as the MMSE (Mini Mental State Examination) and the Montreal Cognitive Function Assessment Scale (MoCA) showed statistically significant improvements in 28 studies. Conclusions: Future research is needed to standardize the treatment of acupuncture, and more diversified high quality papers should be published to help clarify the therapeutic effects of acupuncture and the mechanisms of cognitive disorder post-stroke.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.507-513
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• 2017

Bacillus subtilis spores can be used for protein display to engineer protein properties. This method overcomes viability and protein-folding concerns associated with traditional protein display methods. Spores remain viable under extreme conditions and the genotype/phenotype connection remains intact. In addition, the natural sporulation process eliminates protein-folding concerns that are coupled to the target protein traveling through cell membranes. Furthermore, ATP-dependent chaperones are present to assist in protein folding. CotA was optimized as a whole-cell biocatalyst immobilized in an inert matrix of the spore. In general, proteins that are immobilized have advantages in biocatalysis. For example, the protein can be easily removed from the reaction and it is more stable. The aim is to improve the pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. A library of approximately 3,000 clones was screened. A E498G variant was identified to have a half-life of inactivation ($t_{1/2}$) at pH 4 that was 24.8 times greater compared with wt-CotA. In a previous investigation, a CotA library was screened for organic solvent resistance and a T480A mutant was found. Consequently, T480A/E498G-CotA was constructed and the $t_{1/2}$ was 62.1 times greater than wt-CotA. Finally, E498G-CotA and T480A/E498G-CotA yielded 3.7- and 5.3-fold more product than did wt-CotA after recycling the biocatalyst seven times over 42 h.