• 생명과학회지
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• 제29권8호
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• pp.922-940
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• 2019

비만은 에너지 섭취와 소비의 불균형으로 인해 유발되는 비정상적인 지방 축적으로, 근래에 다양한 만성질환을 초래하는 주요 국제 보건 문제로 부상하였다. 이러한 이유로, 비만 문제에 대한 여러 해결책들이 제시되고 있다. 에너지를 저장하며 내분비 작용을 하는 백색 지방과 달리 열을 생성하여 에너지를 발산하는 두 종류의 지방조직인 갈색 지방과 베이지색 지방이 성인에 존재하며 외부 자극에 의해 유도될 수 있다는 것이 밝혀진 이래로, 이들은 비만 치료의 유망한 표적으로서 각광받고 있다. 이러한 외부 자극 중, 인간 장관계에서 인간과 공존하는 장내 미생물총은 다양한 대사 작용에 참여하며, 이를 조절하는 것이 여러 대사 질환의 치료에 유력한 작용을 할 것으로 보인다. 따라서, 다양한 연구에서 항비만 치료가 장내 미생물 환경 전환이나 갈색 지방 조직 활성화에 미치는 영향에 초점을 맞추고 있다. 본 총설에서는 비만과 체중 증가, 대사 질환을 해소하는 것으로 알려진 자극과, 장내 미생물총의 변화나 갈색지방의 활성화를 야기하는 자극과, 이 자극들과 장내 미생물총의 조작, 지방조직의 갈색화 사이에서 알려져 있거나 있을 수 있는 상관관계를 중점적으로 다루고자 한다.

• 대한한방부인과학회지
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• 제31권4호
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• pp.147-164
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• 2018

Objectives: This study was performed to analyze the pattern identification in infertile women who participated in infertility support program of Cheongju-city. Methods: The project proceeded from October, 2016 to June 2017. Participants filled in the questionnaire which is composed of 33 symptoms before korean medical treatments. Data were collected from 17 participants. We classified symptoms into six pattern identifications based on previous research and rated each answer to score. Results: Throughout the study, the average age of 26 patients was $34.88{\pm}3.19$ years old, the average height was $162.51{\pm}4.99cm$, and average weight was $58.41{\pm}7.88kg$. The average duration of acupuncture treatment was $121.03{\pm}52.73$ days and the average times of acupuncture treatment was $27.42{\pm}10.77$ times. The average number of herbal medicine treatment was $2.65{\pm}0.69$, the majority of it was Chokyungjongok-tang. After the treatment, 3 patients of infertility became pregnant (11.54%) in twenty six patients. By analyzing pattern identification survey papers, We can classify infertile women into 6 pattern identification based on previous research. The majority of participants was identified as Blood deficiency and the other participants were identified as Kidney deficiency, Liver depression, Dampness-phlegm, Qi deficiency and Static blood in order of priority. Most of patients' pattern identification were combined with two kind of pattern identification. In terms of priority, Liver depression combined with Kidney deficiency, Blood deficiency combined with Kidney deficiency, Liver depression combined with Qi deficiency, Liver depression combined with Dampness-phlegm. Conclusions: After the study we confirmed that Korean traditional medicine is a safe treatment for infertile women. Further clinical study about herbal medicines of patients who have combined pattern identification is needed.

• 한국식품과학회지
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• 제38권5호
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• pp.720-723
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• 2006

본 연구에서는 우리 나라 세균성 이질을 유발하는 주요 원인 균인 S. sonnei를 대상으로 불충분한 열처리와 NaCl 처리에 따른 각각의 생존균수 변화와 불충분한 열처리 후 다양한 NaCl 농도 (0, 2, 4, 6%) 하에서 sublethal injured cell의 회복정도를 조사하였다. 그 결과, 불충분한 열처리 정도가 증가함에 따라 생존균수가 유의적인 감소를 나타내었으나, NaCl 처리시 NaCl 농도가 6% 까지 증가함에 따라서 생존균수의 변화가 거의 없었다. 그리고 $55^{\circ}C$에서 30분 동안 열처리후 2% TSAS 배지에서 7.58 log CFU/mL, 4% TSAS 배지에서 7.83 log CFU/mL, 그리고 6% TSAS 배지에서 7.93 log CFU/mL 만큼 각각 생성된 colony 숫자가 감소 한 것으로 조사되었고, $60^{\circ}C$에서 30분동안 열처리후 2% TSAS 배지에서 6.71 log CFU/mL, 4% TSAS 배지에서 6.73 log CFU/mL, 그리고 6% TSAS 배지에서 6.73 log CFU/mL 만큼 각각 생성된 colony 숫자가 감소한 것으로 조사되었다. 그리고 동일한 온도에서 sublethal heating후 노출된 NaCl 농도가 증가함에 따라서 D-value가 감소하는 경향을 나타내었다. 이와 같은 결과는 불충분한 열처리 과정에 의하여 생성된 sublethal injured cell들이 열처리후 회복되는 과정에서 배지의 NaCl 농도가 증가함에 따라서 회복정도가 감소하였음을 의미한다.

• 대한한의학방제학회지
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• 제15권1호
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• pp.49-105
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• 2007

Objectives : Select and analyze the case record of dyspnea and wheezing asthma recorded in Xu Ming Yi Lei An that is the most abundant and wide in contents in existing case records that are systematic, comprehending relatively modern Traditional Chinese Medicine to secure more deep and objective basis of Traditional Chinese Medicine approach for dyspnea and wheezing asthma to analyze and review possibility for clinical application in this study. Methods: The study was conducted with the case records of dyspnea and wheezing asthma in whole Xu Ming Yi Lei An. Pattern identify and classify selected case records and again classified with deficiency syndrome and excess syndrome. Also analyzed prescriptions and herbs used in the case records. Nature of herbs and properties and flavors that were used in the case records were classified and frequency of each nature of herbs were analyzed. Applicable case records were interpreted and suggested prescriptions, pulse feelings, pattern classification were analyzed and described. Results : Among the 5254 case records stated on the complete collection, it is researched that there are 63 case records for the symptom complex of dyspnea as 1.2% of the whole case records, and the case records on the symptom complex of wheezing asthma are 14 as the 0.27% of the total examples. 63 case record examples related with symptom complex of dyspnea were pattern identified and classified. As a result, deficiency syndrome of the Kidney(33 %), deficiency syndrome of the Spleen(26.0%), Wind-Cold(12.3%), phlegm turbidity(12.3%), Heat in the Lung(8.2%), asthenia of the Lung(8.2%) were investigated as above order. 14 case record examples related with wheezing asthma were pattern identified and classified. As a result, phlegm-Heat(26.3%), upper excess and lower deficiency(26.3%), external affections Wind-Cold(15.8%), Dampness-phlegm(10.5%), Lung asthenia(10.5%), Cold phlegm(5.3%), mutual deficiency and detriment of Heart and Kidneys(5.3%) were investigated as above order. Symptom complex of dyspnea has 67.1% of deficiency syndrome, 32.9% of excess syndrome resulting more deficiency syndrome than excess syndrome. Symptom complex of wheezing asthma has 42.1 % of deficiency syndrome and 57.9% of excess syndrome resulting more excess syndrome than deficiency syndrome. In case of symptom complex of dyspnea prescription used in the case record, the order of frequency is as following. Palmijihwang-tang, Bojung-ikgitang, Yungmijihwang-tang, Ijintang, Sojaganggitang, Igongsan. In case of symptom complex of wheezing asthma prescription in the case record, Yungmijihwang-tang, Ohotang, Dodamtang were mostly used. Herbs used in case records of symptom complex of dyspnea are Ginseng Radix, Poria, Glycyrrhizae Radix, Aconiti Iateralis Preparata Radix, Atractylodis Macrocephalae Rhizoma, Dioscoreae Rhizoma, Angelicae Gigantis Radix, Rehmanniae Radix Preparat, Pinelliae Rhizoma, Zingiberis Rhizoma Recens are mostly used. Nature of herb properties used for symptom complex of dyspnea and symptom complex of wheezing asthma are herbs that are warm properties. When the symptom complex of dyspnea and the symptom complex of wheezing asthma were treated. if the patient felt tenderness at Pyesu, doctors conducted pricking blood around the opposite Pyesu or Sipseon acupoint. when the patient didn't have tenderness at Pyesu by soft press, pricking blood was performed both sidees, right and left Pyesu. In case of the treatment of symptom complex of dyspnea and symptom complex of wheezing asthma, when they got treatment, when the symptom complex of disease is severe, a doctor cauterized the opposite Pyesu while the other Pyesu felt tenderness, and decided how the above treatment is performed whether the degree of the symptom compolex of disease is severe or not. In case of the treatment of symptom complex of dyspnea and symptom complex of wheezing asthma, if the person felt tenderness at Pyesu and is caught by the Wind-Cold pathogen, slight acupuncture is treated at relevant Pyesu with Fire needling. When patient with symptom complex of dyspnea and symptom complex of wheezing asthma cannot hawk sputum up from the oral and laryngopharynx, suction method is treated. Conclusion : With this study, actual traditional and clinical pattern identification form and characteristics of symptom complex of dyspnea and symptom complex of wheezing asthma were recognized. Modern case report utilizing in clinical application need to be secured and an incurable disease asthma need to be diagnosed and improvement for treatments have to be searched through other case records.

• 생명과학회지
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• 제30권5호
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• pp.460-467
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• 2020

간암은 전 세계적으로 가장 높게 진단되는 암 중 하나이며, 방사선 및 화학 요법이 일반적으로 사용되는 치료법이지만 다양한 부작용은 치료 효과를 크게 제한한다. 따라서 전통 의학에서 사용되어 온 처방법은 이를 극복할 수 있는 대안이 될 수 있다. 본 연구에서는 동의보감에 기술되어 있는 3가지 한약 처방전(비기환, 대칠기탕 및 목향빈랑환)을 선택하여 인체 간암세포에 대한 항암 효과를 평가하였다. 간암세포에서 3가지 처방전의 에탄올 추출물의 세포 독성을 조사하기 위한 MTT 분석 결과, 비기환 추출물은 대칠기탕 및 목향빈랑환에 비하여 세포 생존력을 현저하게 억제하였다. 그리고, flow cytometry 분석의 결과에서 3가지 처방전 추출물에 의한 간암세포의 증식 억제가 apoptosis 및 autophagy 유도와 관련이 있었다. 특히, 비기환 추출물은 미토콘드리아의 기능을 크게 손상시켰으며 다른 두 처방전과 비교하여 mitophagy 유발 가능성을 보여주었다. 아울러 비기환 추출물은 LC3의 발현을 현저하게 증가시켰으며, 이는 Bcl-2의 발현 감소를 동반하는 반면, Bax의 발현에 영향을 미치지 않았다. PINK1의 발현 또한 비기환 추출물이 처리된 세포에서 매우 증가하였다. 나아가, autophagy 억제제는 3가지 처방전 추출물 처리에 의한 세포 생존율 감소와 apoptosis 유도를 억제하였으며, 이러한 결과는 이들 처방전 추출물 처리에 의한 autophagy가 apoptosis 개시에 관여하고 있음을 보여주는 것이다. 결론적으로, 본 연구 결과는 비기환 추출물이 3가지 처방전 중에서 가장 높은 항암 활성을 보였으며, 비기환 추출물은 autophagy 유도제로서 간암세포의 증식을 억제함을 의미한다.

• 대한한의학회지
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• 제31권4호
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• pp.38-48
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• 2010

Objectives: Moxa treatment has outstanding effects that are frequently facilitated in a clinical setting, and it is also known to have positive effects for immunity and relief of pain through various studies. Currently, moxa treatment has become diversified in many ways. One of the moxa treatment techniques that was frequently used during the Joseon Dynasty was Woogakgubeop (牛角灸法, horn-shaped moxa treatment). This articles aims to disclose the fact that the Woogakgubeop is the technique applicable to the origin of the Large moxa treatments of today. Also, the writings of Woogakgubeop recorded in the royal medical documentations, such as the Chronicles of the Royal Families of the Joseon Dynasty, the Seungjeonwonilgi (The Daily Records of Royal Secretariat) and others, are analyzed and the characteristics of the Woogakgubeop are cleared through it. Methods: From the royal medical documents, the Chronicles of the Royal Families of the Joseon Dynasty and the Seungjeonwonilgi, articles related Woogakgubeop were extracted and analyzed. Records of each document were facilitated for the DB search. In addition to these two documents, the records of written literature were surveyed as well. The written literature are appropriate to express the experiences of intellectuals at the time. Results and Conclusions: 1. Considering that Woogakgubeop is a single therapy, there exists relatively many records in addition to the specialized medical documents, and through them, it is known that Woogakgubeop was a widely known therapy in the middle to later parts of Joseon in general. 2. Woogakgubeop is a method that addresses problems contained within the existing moxa treatment techniques. By enlarging the size of the mugwort wick, it strengthens the heat energy of the moxa treatment to reduce the frequency of applying moxa treatment. When undertaking the method to empty the wick inside the mugwort, the pain of a fever patient will be reduced and burn damage to the skin is minimized. 3. Wind-cold type weakness and other general moxa treatment techniques have been used for various symptoms detailed, including chest pain, tinnitus, carbuncle, cellulites, cold sense of leg, colic, diarrhea and other illnesses. In addition, it may generate strong fever, and was used to implement the Yeonjebeop (煉臍法). 4. Woogakgubeop is applicable to the origin of Large moxa treatment of today and it provides important bibliographic base thereof.

• 대한본초학회지
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• 제25권2호
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• pp.129-136
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• 2010

Objective : Sinapis alba L. (SA) is a korean traditional herbal medicine that is usually used to prevent or treat inflammatory diseases, such as respiratory infection and rheumatoid arthritis. However, the effects of SA supplementation in vitro on serum antibody levels, splenocyte and peritoneal macrophage immune responses have not yet been determined. In this study, we examined the effect of SA on the production of Th1/Th2 cytokines. Methods : Splenocytes were isolated from naive C57BL/6 mice. Cells were enriched for CD4+ cell populations by first staining the cells with anti-CD4 (BD PharMingen, Calif, USA). CD4+ T cells were selected on a (CS) column, and the flow-through was collected as CD4+ T cells. Isolated cells were activated by overnight incubation on 24-well plates coated with $1{\mu}g/mL$ anti-CD3, $1{\mu}g/mL$ anti-CD28 and with SA ($100{\mu}g/mL$). Primary macrophages were collected from the peritoneal cavities of mice (8-week-old female C57BL/6). The peritoneal macrophages were washed and plated with RPMI-1640 overnight for the experiments. After 48-hours cultures, samples were centrifuged at 2000 rpm for 10 minutes, and the supernatants were stored at $-80^{\circ}C$. Mouse IL-4, IFN-$\gamma$ and TNF-$\alpha$ were quantified using ELISA kits (BioSource International, Camarillo, Calif, USA) according to the manufacturer's protocols. Results : SA at 100ug/ml decreased the generation of Th1 cytokine (IFN-$\gamma$) by 0.5-fold. However, SA has no effect on Th2 (IL-4) production. Conclusions : These results suggest that SA may play an important role in the control of T-cell-mediated autoimmunity by down-regulation of Th1 cytokine (especially IFN-$\gamma$, TNF-$\alpha$). These data may contribute to the design of new immunomodulating treatments for a group of autoimmune diseases.

• Korean Journal of Acupuncture
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• 제34권3호
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• pp.164-171
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• 2017

목적 : 본 연구는 음허로 인한 야간발열 환자 2명에 대한 동씨침의 영골 및 대백혈의 치료효과를 알아보고, 이를 경락학적으로 고찰하는데 목적이 있다. 방법 : 음허로 진단된 2명의 환자를 대상으로 영골 및 대백혈에 침 치료를 시행하고, 15분 간 유침하였으며, 치료 전 후에 체온과 맥박을 측정하고, 구갈에 관한 NRS를 치료전에 측정하였다. 결과 : 영골 및 대백혈 자침 1시간 후 체온이 두명의 환자 모두 정상범위로 하강하였으며, Case 1의 경우는 영골 및 대백혈 자침치료를 시작한지 13일후, Case 2의 경우에는 14일 후에 야간발열 증상이 소실되었다. 11 pm에 측정한 맥박수는 Case 1에서 침 치료 시작 전 평균 82.6에서 치료 기간 중에는 75.2, 치료 후 74.8로 변화하였고, Case 2에서는 침 치료 시작전 평균 83.5에서 치료 기간 중에는 78.5, 치료 후 74.3으로 변화하였다. NRS는 Case 1에서 치료 전 평균 4.2에서 치료 기간 중에는 3.2, 치료 후 1.7로 감소하였으나 Case 2에서는 치료 전 평균 3.3에서 치료 기간 중에는 2.3, 치료 후 2.4로 변화하였다. 결론 : 두 가지 Case에서 영골 및 대백혈 자침은 발열증상을 완화하는데 효과적이었다. 이는 영골 및 대백혈이 수양명대장경의 경락 및 경근이 분포하는 영역에 위치하고 있기 때문에 수양명대장경의 열성병 치료효과와 대장주진의 생리학적 관점에서 고찰해 볼 수 있으며, 앞으로 이와 관련한 체계적이고 다양한 연구가 필요하리라 사료된다.

• 한국버섯학회지
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• 제13권4호
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• pp.314-318
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• 2015

본 연구는 지역 특산물인 감과피를 이용한 버섯배지 개발을 위한 기초자료로서 감과피의 이용 가능성을 검토하고자 수행되었으며 감과피는 경상남도 산청지역에서 곶감을 생산하고 남은 것을 수거하여 건조한 후 사용하였고 공시균주는 ASI 2312를 사용하였다. 대조구는 혼합배지:콘코브:톱밥(220:65:15, v/v)을 혼합한 기본배지를 사용하였고 기본배지 중 혼합배지를 5%, 10%, 15%, 20%, 30%, 40%, 50%의 건조된 감과피 처리구로 대체한 처리구를 시험구로 사용하였다. 대조구인 기본배지에 비해 시험구인 5%, 10%, 15%, 20%, 30%, 40%, 50% 감과피 처리구의 T-N 함량은 감과피 첨가량이 증가할수록 감소하는 경향을 나타내었으며 T-C 함량은 큰 차이가 없었고 C/N율은 감과피 첨가량이 증가할수록 증가하는 경향을 나타내었다. 감과피 첨가량이 균사생장에 미치는 영향을 조사한 결과 대조구보다 감과피가 첨가된 모든 시험구에서 균사생장이 느리게 나타났다. 감과피 첨가량에 따른 배양일수는 대조구인 혼합배지에 비해 감과피를 30%까지 첨가하여도 배양일수와 초발이 소요일수에는 뚜렷한 차이는 없었지만 감과피 첨가량이 증가할수록 배양일이 길어지는 경향을 보였으며 혼합배지에 비해 감과피 첨가비율이 증가할수록 갓의 직경은 감소하고 대의 길이는 길어지고 직경은 감소하는 경향을 나타내었다. 수확 후 동결 건조한 큰느타리버섯의 수분, 단백질, 조회분 함량은 대조구와 시험구에서 유의성 있는 차이를 나타내지 않았지만 조지방 함량은 감과피 첨가량이 증가함에 따라 증가하는 경향을 나타내었고 ${\beta}-glucan$ 함량은 대조구에 비해 10%와 15% 감과피 첨가구에서 높게 나타났다. 따라서 대조구와 비교했을 때 15% 감과피 첨가구는 자실체 수량도 높게 나타나고 ${\beta}-glucan$ 함량도 높게 나타났기때문에 감과피 첨가비율은 15%가 가장 적합한 것으로 사료된다.

• 한국약용작물학회지
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• 제21권3호
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• pp.179-183
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• 2013

This study was carried out to investigate the variation of growth and yield using different planting densities and vinyl mulching in S. miltiorrhiza cultivation. Top plant growth was observed in the $30{\times}30cm$ planting density of S. miltiorrhiza;, plant height, leaves and branches were larger than in the other treatments. Root length, root diameter, and supporting roots of underground part were increased as planting density was wide, and dry root weight was increased in $30{\times}30cm$. However, yield was highest at 294 kg/10a in the planting density of $30{\times}10cm$. The use of 30-31cm white and black vinyl mulching had no significant effect on the plant height of S. miltiorrhiza, as compared to non-mulching. However, the highest volume of leaves and plant weight was observed in plants using 24.7 black vinyl mulching. Dry underground root weight was highest in black vinyl mulching at 21.7 g compared to 17.0 g for non-mulching. Yield per 10a increased by 28% using black vinyl mulching compared to non-mulching. Based on the results of this study, planting density of $30{\times}10cm$ and black vinyl mulching are the most suitable in the cultivation of S. miltiorrhiza.