• Reproductive and Developmental Biology
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• 제33권2호
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• pp.113-117
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• 2009

Animals produced by somatic cell nuclear transfer (SCNT) using genetically modified cells are almost always transgenic, implying that this method is more efficient than the traditional pronuclear microinjection method. Most somatic cells for SCNT in animals are fetus-derived primary cells and successful gene integration in somatic cells will depend on transfection condition. The objective of this study is to evaluate the efficiency of electroporation (Microporator) and liposome reagents (F-6, F-HD, W-EX, W-Q, W-M) for tissue-type plasminogen activator (tPA) gene transfection and to estimate the overall efficiency of transfection of Korean native pig fetal fibroblast cells (KNPFF). Electroporation showed significantly higher transfection efficiency than liposome reagents with regard to the transfection of in vitro cultures in the early stages of development (41.7% with Microporator vs. 18.3% with F-6, 20.0% with F-HD 18.5% with W-EX, 5.0% with W-M and 6.3% W-Q,). Colonies identified as tPA-positives were treated once more with G418 for 10 to 14 days and growing colonies were selected again. When the cells of newly selected colonies were subjected to single-cell PCR, reselection of colonies following second round of G418 selection increased the rate of transgene integration per each colony. These results suggest that transfection with electroporation is the most efficient and the second rounds of G418 selection may be an effective method for transfection of porcine fetal fibroblast cells.

• 한국농촌건축학회논문집
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• 제20권2호
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• pp.49-56
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• 2018

The vernacular houses with 田-shaped floor plan at Gangreung have been played important roles in tracing the vernacular living cultures including housing life. But due to the adoption of convenient facilities in vernacular housing lives caused by modernization and urbanization, many changes and transformations have happened at residential space of vernacular houses. This thesis targets to extract basic data to prepare and prevent various problems in process of renovation, extension and function change as installing housing life facilities, and to identify the changes and transformations of them to prepare the proper balances between their original form preservation and residents' convenience living. The results are as follows. The inner wall removal of rooms, the fuel change of cooking and heating, the leveling change of kitchen floor made possible the sedentary lifestyles. And its residential rooms were transformed from two row to one row by inner wall removal, the fuel heating change, the heating method change and the heat insulating materials. The residential extension materials were changed from the traditional materials such as soil, wood, etc to the modern materials such as cement block or brick, sandwich panel, etc. And the modern materials were used and attached to widen residential spaces to the vernacular house because of the easiness of execution. So, it is necessary for 田-shaped floor plan vernacular house owners to distribute printed execution guide book to prevent and minimize the various problems to be shown by extension, renovation and function change.

• 한국미생물·생명공학회지
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• 제45권4호
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• pp.305-310
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• 2017

발효식품으로부터 분리한 젖산균 중 ${\alpha}$-rhamnosidase 효소활성을 보유한 젖산균 12점을 선별하였다. 효소활성이 우수한 Weissella confuse 1점, L. pentosus 1점과 효소활성이 우수하였던 4점의 L. plantarum 균주를 사용하여 진세노사이드 Rb1과 Re를 Rg1, Rg5로 각각 생물전환하였다. L. plantarum MBE/L2990 균주를 사용한 생물전환반응에서 Rg1과 Rg5의 함량이 각각 0.58 mg, 0.24 mg 가량 증가하였으며, 대조구로 사용한 L. plantarum KCTC21004 균주에 비해 약 56% 및 42% 우수한 생물전환 효율이었다. L. plantarum MBE/L2990 균주는 한국미생물자원센터에 KCTC18529P로 기탁하였다.

• 한국콘텐츠학회논문지
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• 제18권5호
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• pp.11-22
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• 2018

본 연구에서는 4차 산업혁명 시대, 변화하는 공공도서관의 역할과 대응방안을 보여주는 사례로 독일 쾰른 시립도서관이 지역사회 이용자들에게 제공하는 다양한 프로그램의 구성 및 내용을 살펴보고 그 시사점에 대해 논의한다. 개관이래 지속적으로 독일 도서관 교육의 선도적 역할을 수행해 온 쾰른 시립도서관은 4차 산업혁명이라는 역동적인 시대의 흐름에 맞추어 정보와 지식을 생산하고 문화를 창달하는 중심지가 되기 위해 스스로 변화하고 있다. 도서의 수집 및 보존이라는 도서관의 전통적인 기능을 넘어, 쾰른 시립도서관은 현재 지역 구성원들이 영화, 음악, 게임 등 다양한 문화들을 체험하고 문화를 창조할 수 있는 지역사회의 복합문화공간의 역할을 담당하고 있다. 독일 쾰른 시립도서관의 프로그램은 향후 한국의 공교육 차원에서 한국 공공 도서관의 기능과 역할을 재구상하고 지역 구성원을 위한 도서관 콘텐츠를 기획하는 데에 도움이 될 수 있을 것이다.

• 대한임상독성학회지
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• 제10권1호
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• pp.1-7
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• 2012

Purpose: Herbal preparations have long been used for medical purposes by traditional cultures, and their use is increasing in modern societies. However, many herbal agents produce specific cardiovascular toxicities in humans. We performed this study in order to investigate the clinical characteristics of the cardiac toxicities associated with herbal remedies. Methods: We conducted a retrospective study of 45 patients (mean age $57{\pm}10$ years) who presented with cardiotoxicity between January 2007 and May 2011 due to ingestion of herbal remedy substances. Patients were identified as suffering cardiotoxicity if they presented with chest pain, EKG abnormality, and elevation of cardiac enzyme. Results: Of the 45 total cases, 17 included hemodynamic instability (37.8%), 7 with increasing cardiac enzyme (15.6%), 2 with cardiac arrest (4.4%) and one case of mortality (2.2%). The cardiotoxic herb group that demonstrated the worst clinical course was Ranunculaceae. Conclusions: In our study results, 57.6% of the herbal intoxication patients demonstrated the effects of cardiotoxicity. Thus, we recommend careful monitoring of herbal intoxication patients.

• 마케팅과학연구
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• 제17권4호
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• pp.223-245
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• 2007

Purpose: While empirical importance of dimensionality of perceived value is widely accepted, our understanding of experiential value dimensions in other retail channels and other cultures has not been explicitly tested. This study attempted to determine if the dimensions of experiential value scale (EVS) by Mathwick, Malhotra, and Rigdon (2001) identified in US catalog and Internet contexts could be applied in other international markets (South Korea) and in other retail channels (department store versus Internet shopping mall). Methodology/Approach: Two data sets, one from 220 department store shoppers and the other from 359 Internet shopping mall shoppers, were analyzed. Findings: Confirmatory factor analysis confirmed four different EVS dimensions by retail channels. Overall, entertainment and intrinsic enjoyment values were found to be more important in department store while economic and efficiency value dimensions were interpreted critical in Internet shopping mall context. Visual appeal aspect constitutes distinct value dimension in two channels. Practical Implications: One separate dimension of time efficiency in Internet shopping mall suggests that more efficient web design and functions that can save time and promote convenience are needed to better accommodate their customers. Internet has heavily relied on traditional attributes, such as factual information, price comparability, and brand name reliance. However, this study suggests that Internet shopping mall retailer should offer visual diversion and stimulation just as brick and mortar shopping malls do. Originality /Value of Paper: Although the research findings must be viewed as tentative because the results are from one country, they provide a rich basis for further understanding the dimensions of experiential value in other international markets and other retail channels. Category: Research Paper

• 가족자원경영과 정책
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• 제14권3호
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• pp.109-122
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• 2010

The present study aims to investigate cultural similarities and differences that influence interracial couples (between Korean men and Vietnamese women) when adapting to Korean family culture in terms of interculturalism. The interviewees consisted of three generations. This study used in-depth interviews. Data was collected from January to August 2008. 15 families (45 people) were used for analysis. Results were as follows: (1) the patriarchal culture of the two nations had a positive influence on the adaptation of members during family interactions(2) the culture of filial piety is a value system that both nations believe is important. However, different rituals had a negative influence on the practice of filial piety (3) the community-oriented culture is regarded as important in the two nations. However, Vietnamese wives cannot lead a free community life because Koreans see them in negative terms (4) the Vietnamese traditional wedding custom called "NopJjeOh" had a negative influence on the adaptation to the Korean family culture (5) the preference for sons in Vietnam is not as high as in Korea. This difference had a negative influence on adaptation (6) clear differences between the daily lives of the two nations had a negative influence on adaptation to Korean family culture. In conclusion, Korea and Vietnam have many similarities in culture, which enable Vietnamese wives to adapt to Korean culture more easily than other foreign wives. If various programs are developed to promote the mutual understanding between both cultures based on these research findings, it would further contribute toward social integration in Korea.

• Advances in Traditional Medicine
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• 제1권1호
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• pp.55-63
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• 2000

Soeumin Sibjeundaibo-tang (SJDBT) and Soyangin Sibimijihwang-tang (SMJHT) have been used traditionally to improve the systemic blood circulation and biological energy production in the patients with circulatory and neuronal diseases. The object of this study is to determine the protective effects of SJDBT and SMJHT extracts on the spontaneous and glutamate-induced neuronal damages in cultured cells derived from mice cerebral cortex. At 14 days after beginning the cultures, the activity of lactate dehydrogenase released into the culture media was significantly decreased by treatment of cerebroneuronal cells with SJDBT and SMJHT (0.1 mg/ml) for 7 days. By comparison with the normal cells, cerebroneuronal morphology was dramatically changed by treatment of glutamate (1 mM) for 12 hrs, and this was conspicuously recovered by pretreatment of cerebroneural cells with SJDBT and SMJHT (0.1-1.0 mg/ml) for 2 days. Moreover, glutamated-induced DNA fragmentation was also protected by pretreatment of cerebroneuronal cells with those extracts. These results suggest that naturally occurring and glutamate-induced degeneration of cultured cerebrocortical cells may be related, in part, to the process of apoptotic cell death. The pharmacological properties of SJDBT and SMJHT extracts to improve cerebroneuronal degeneration may be considered as one of useful medicines that can prevent cerebrocortical impairments resulted from age-dependent and excitotoxicity-induced neuronal degeneration in human brain.

• 건축역사연구
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• 제13권3호
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• pp.97-117
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• 2004

The tradition of architecture in the East and the West are different in many ways. One of the basic cause of such difference, however, is the different ways of perception of space and time. This paper aims to identify the attitude of perception of space and time in the cultures of the East and the West, and its influence on architecture. Degrees of importance placed on either space or time, as well as the modes of perception of space and time are discussed in relation to architecture. Basically, the architecture of the West seems to be more spatially oriented than the East, and this have much to do with the fact that the tradition of Western architecture is visually oriented. On the other hand, East Asian architecture have been more conscious on bodily feeling and its movement in architecture. Spatial units of traditional Eastern architecture, are arranged in such a way in which man can experience the change of space that is supposed to be organized to form a sequential message. Thus, in the East, temporal dimension is more deliberately included in the course of architectural experience compared to the cases of the West. Although it is not easy to attempt any kind of value judgment on such aspects, it is necessary to understand how the different perception of space and time influenced architectural outcome, especially when one wants to understand the cultural cause that have made the architectures of the East and the West very different. Such understanding is particularly important in East Asia where their future of architecture depends much on how they harmonize the Eastern and Western background which are already built up as two cultural structure in their consciousness to be able to create more desirable architecture for themselves.

• 건축역사연구
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• 제19권4호
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• pp.35-54
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• 2010

This study aims to examine the development process of the early stone pagodas of Unified Silla. The history of stone pagodas traces back to both Baekje and Silla in the Three Kingdoms period, but the styles and structures of pagodas began differently. In other words, Baekje attempted to recreate the wooden pagoda style, while Silla attempted to recreate China' brick pagoda style. These different stone pagoda cultures, around the unification of Three Kingdoms, underwent new style changes, and after the mid-7th century, some changes in the five-storied stone pagodas in Tapri appeared, and the milestone perfection of Silla's stone pagodas was achieved through those of Gameusa Temple and Goseonsa Temple. After the mid-7th century, Silla's stone pagodas accommodated some of Baekje's wooden pagodas' elements, shifted from the wooden pagoda style and developed into its own stone pagoda style. This is shown in numerous stone pagodas. However, in Silla's stone pagodas, including the three-storied stone pagoda in Hwagboksa Temple in 692, the size of the pagoda became small and underwent sudden changes. In other words, a new direction of Silla stone pagodas was presented in terms of how massive stones could be reduced, but they differed only in the reduced stone amount; the basic developed style of the Gameunsa Temple stone pagoda and the Goseonsa Temple stone pagoda inherited the traditional style. Thus, the construction of these two pagodas is considered to be significant in the Silla's history of stone pagodas.