• Bulletin of the Korean Chemical Society
• /
• v.29 no.2
• /
• pp.422-426
• /
• 2008

Hemodialysis graft coated with paclitaxel prevents stenosis; however, large initial burst release of paclitaxel causes many negative effects such as drug toxicity and inefficient drug loss. Therefore we developed and tested a novel coating method, double dipping, to provide controlled and sustained release of paclitaxel locally. Expanded polytetrafluoroethylene (ePTFE) grafts were dipped twice into a solution of several different paclitaxel concentrations. In vitro release tests of the double dipping method showed that early burst release could be somewhat retarded and followed by sustained release for a long time. We observed the effect of paclitaxel coating by double dipping in porcine model of arterio-venous (AV) grafts between the common carotid artery and the external jugular vein. 12 weeks after constructing AV grafts, cross sections of the graft venous anastomosis were obtained and analyzed. Paclitaxel coated ePTFE grafts by double dipping were observed to prevent neointimal hyperplasia and therefore reduced stenosis of the arteriovenous hemodialysis grafts, especially at the graft venous anastomosis sites. Our results demonstrate that second dipping of ePTFE graft, which was already coated once with paclitaxel, washes off the drug on a surface of the graft and affects the ratio of paclitaxel on the surface to that of the inner space, possibly by diffusion: thus the early burst of drug can be somewhat reduced.

• Journal of Korean Society of Water and Wastewater
• /
• v.21 no.1
• /
• pp.113-122
• /
• 2007

Experimental studies were performed to investigate speciation and leaching potential of heavy metals(Cu, Cd, Pb Zn, Ni) in ten sediment samples collected from Nakdong River. Acid Volatile Sulfide (AVS) and Simultaneously Extractable Metals (SEM) measurements were used to estimate heavy metals that can be leached under anaerobic conditions. Sequential Extraction Procedure (SEP) and Toxicity Characteristic Leaching Procedure (TCLP) were used to characterize speciation and leaching potential of heavy metals under aerobic conditions. The results show that total concentrations of Cu, Zn and Ni were relatively high in the sediments from points Seongseo industrial complex stream (upper stream) (D), Seongseo industrial complex stream (midstream) (E), Dalseo stream (F), and Nakdong river estuary (J), and that Cd concentrations were higher in all sampling points except for Goriung Bridge (G) and Soosan Bridge (H). SEM and AVS analyses reveal that samples from points Ilsun Bridge (A), Namgumi Bridge (C), and Soosan Bridge (H) have potential of heavy metals leaching, although leachable concentrations are relatively low. The leaching potential of heavy metals in other points was low because of higher concentrations of AVS than SEM. SEP results show that more than 50% of Cu, Zn and Ni were present in residual fraction, which means these metals are less amenable to leaching in anaerobic conditions. On the other hand, more than 50% of Pb and Cd were extracted during the first through third step of the SEP, which means substantial fraction of these metals can be leached upon changing of redox conditions. TCLP tests predict that leaching potential of heavy metals was generally low, which is consistent with the results obtained by AVS and SEM measurements.

• Food Science and Preservation
• /
• v.8 no.1
• /
• pp.54-59
• /
• 2001

The three medicinal herbs, Astragali Radix, Glycyrrhizae Radix and Aurantii nobilis Pericarpium, irradiated with γ-rays have been tested for their possible genotoxicity. The hot water extracts of the 10 kGy γ-ray irradiated herbs were examined in the Salmonella mutagenicity test(histidine reversion assay; Ames test) using Salmonella. typhimurium TA98 and TA100 as tester strains. No mutagenicity was detected in this assay both with and without metabolic activation. The safety of the herbs irradiated with γ-rays at tactical doses needs to be evaluated in further tests of genotoxicity in vivo and chronic and reproductive toxicity.

• Journal of Korean Medicine Rehabilitation
• /
• v.30 no.2
• /
• pp.47-66
• /
• 2020

Objectives The purpose of this study is to evaluate the bone healing effect of Dohongsamul-tang (Taohongsiwu-tang; DH) on femur fractured mice. Methods Mice were randomly divided into 4 groups (naive, control, positive control and DH). All groups except naive group were subjected to bone fracture on both hind limb femurs. Naive group received no treatment at all. Control group was fed with normal saline, and positive control group was orally medicated with tramadol. DH-treated group was orally medicated with DH. We analysed the levels of BMP2, COX2, Col2a1, Sox9, Runx2, and Osterix genes on 3, 7 and 14 days after fracture. Alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, creatinine, total cholesterol, and triglyceride levels were measured for safety assessment. Results In morphological, histological analysis, callus formation process of DH-treated group was faster than the control group. BMP2, Sox9 gene expression were significantly increased at 7 days after fracture compared to the control group. COX2, Col2a1 gene expression were significantly increased at 14 days after fracture compared to the control group. Total cholesterol was significantly increased by DH at 3 days. Triglyceride was significantly decreased by DH at 3, 7 days after fracture compared to the control group. Conclusions Dohongsamul-tang promoted bone healing process after fracture by stimulating the bone regeneration factors. And DH shows no hepatotoxicity, nephrotoxicity and serum lipid abnormality. In conclusion, it seems that DH helps to promote fracture regeneration after bone fracture by regulating gene expressions related to bone repair.

• Journal of Fashion Business
• /
• v.24 no.3
• /
• pp.30-39
• /
• 2020

This study was conducted to identify problems in domestic flame-retardant performance specifications. Currently, the domestic wallcovering anti-inflammatory regulations are not prepared for damage caused by smoke, with the carbonized area as the main function. In particular, given that smoke is the main cause of human casualties and injuries in a fire, it is reasonable that the flame density and toxicity of the wallcovering should also be the main performance indicators. The scope and method of research in this study were as follows. First, a prior study related to fire on various wallcoverings was considered. Second, it raised questions about the effects of smoke in the event of a fire and domestic anti-inflammatory performance tests. Third, textile wallcovering samples were manufactured with viscose rayon for experimental verification of the problems and tested by Korean and EU standards without flame retardant processing to analyze the differences between each regulation. Fourth, the performance of flame retardant wallcovering according to Korean standards was evaluated using smoke density and harmful gas testing methods. The results of each test were as follows. Non-fire retardant wallcovering was rejected by Korea standards. However, B-s1.d0 in Europe. Smoke density testing and harmful gas by domestic combustion processing on the same sample showed that the smoke density increased about 4.3 times more than before, and the harmful gas test showed that the suspension of the post-processing sample slowed earlier than the non-processed sample.

• Journal of Pharmacopuncture
• /
• v.14 no.3
• /
• pp.19-45
• /
• 2011

Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30 descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results: 1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.

• Journal of Pharmacopuncture
• /
• v.20 no.4
• /
• pp.280-286
• /
• 2017

Objective: This case series aims to report the efficacy and the safety of using snake venom pharmacopuncture (SVP) for chemotherapy-induced peripheral neuropathy (CIPN). Methods: Three heterogeneous cancer (1 endometrium, 1 cervix, and 1 prostate cancer) patients were referred to the East-West Cancer Center (EWCC), Dunsan Korean Medicine Hospital of Daejeon University, from August 02, 2017, to September 15, 2017, for treatment with SVP, and they were treated with SVP 4 times, 6 times, and 8 times, respectively. During the treatment period, the efficacy of SVP therapy was assessed by using the Numerical Rating Scale (NRS) and the Common Terminology Criteria for Adverse Events (CTCAE), and the stability was evaluated by using blood tests. Following each session, all patients were examined closely for any allergenic responses or adverse effects. Results: All patients showed noticeable improvements of their NRS and CTCAE scores. Except for bleeding and bruising at the SVP injection site, no major side effects were noted. One of the patients reported mild chilling and a sore throat after receiving the second treatment; those symptoms went away after a few hours. No hematologic toxicity, hepatotoxicity, or nephrotoxicity was found on the blood test. Conclusion: The results of this research suggest positive potential benefits of using SVP for treating patients with CIPN. Also, the excellent safety results of SVP seen in this research should lead to larger clinical trials aimed at developing SVP into a potential intervention for managing patients with the symptoms of CIPN.

• Journal of Microbiology and Biotechnology
• /
• v.7 no.2
• /
• pp.144-150
• /
• 1997

We developed a simple and efficient method to prepare a Pseudomonas vaccine of outer membrane (OM) proteins free from lipopolysaccharide (LPS). A three step purification process including extraction, ultrafiltration and ultracentrifugation effectively removed LPS from the OM protein fraction. Approximately 2 mg of the OM proteins was obtained from 1 g of wet cell. LPS contaminant in the vaccine preparation was less than 0.003% (w/w) of protein and protease activity was not detectable. To achieve a wide range of protection, OM proteins prepared from four attenuated P. aeruginosa strains were mixed in equal amounts and used as a vaccine, which elicited in rabbits a high titer of antibody reactive to all of the seven Fisher types. The antisera from the immunized rabbit had a strong reactivity to vaccine proteins larger than 25 kDa. In a burned mouse infection model, immunization with the vaccine significantly enhanced bacterial clearance in the Pseudomonas infected skin. The vaccination also provided mice an excellent protection against Pseudomonas infection (11, 16). Data on antigenicity, mutagenicity, acute, subacute toxicity and pharmacological tests confirmed the safety of the vaccine (1, 3, 10, 12, 17). These data demonstrate that this method can be applied to manufacture a bacterial vaccine of OM proteins with safety and prophylactic efficacy at a practical low cost.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 1999.04a
• /
• pp.149-156
• /
• 1999

In the United States (U.S.), the monitored natural attenuation (MNA) approach has been used as an alternative remedial option for organic and inorganic compounds retained in soil and dissolved in groundwater. The U.S. Environmental Protection Agency (EPA) defines the MNA as“in-situ naturally-occurring processes include biodegradation, diffusion, dilution, sorption, volatilization, and/or chemical and biochemical stabilization of contaminants and reduce contaminant toxicity, mobility or volume to the levels that are protective of human health and the environment”. The Department of Soil Environment. National Institute Environmental Research (NIER) is in the process for demonstrating the MNA approach as a potential remedial option for the BTEX contaminated site in Uiwang City. The project is charactering the research site in terms of the nature and extend of contamination, biological degradation rate, and geochemical and hydrological properties. The microbial-degradation rate and effectiveness of nutrient and redox supplements will be determined through laboratory batch and column tests. The geochemical process will be monitored for determining the concentration changes of chemical species involved in the electron transfer processes that include methanogenesis, sulfate and iron reduction, denitrification, and aerobic respiration. Through field works, critical soil and hydrogeologic parameters will be acquired to simulate the effects of dispersion, advection, sorption, and biodegradation on the fate and transport of the dissolved-phase BTEX plume using Bioplume III model. The objectives of this multi-years research project are (1) to evaluate the MNA approach using the BTEX contaminated site in Uiwang City, (2) to establish a standard protocol for future application of the approach, (3) to investigate applicability of the passive approach as a secondary treatment remedy after active treatments. In this presentation, the overall picture and philosophy behind the MNA approach will be reviewed. Detailed discussions of the site characterization/monitoring plans and risk-based decision-making processes for the demonstration site will be included.

• Journal of Korean Society of Water and Wastewater
• /
• v.25 no.1
• /
• pp.1-5
• /
• 2011

Arsenic is one of the most abundant contaminant found in waste mine tailings and soil around refinery, Because of its carcinogenic property, the countries like United States of America and Europe have made stringent regulations which govern the concentration of arsenic in soil. The study focuses on solidification/stabilization for removal of arsenic from soil. Cement was used to solidify/stabilize the abandoned soil primarily contaminated with arsenic (up to 68.92 mg/kg) in and around refinery. Solidified/stabilized (s/s) forms in the range of cement contents 5-30 wt % were evaluated to determine the optimal binder content. Revised Korean standard leaching tests (KSLT), toxicity characteristic leaching procedures (TCLP), Old Korea standard leaching test and revised Korea standard leaching test were used for chemical characterization of the S/S forms. The addition of 10 % cement remarkably reduced the leachability of arsenic in contaminated soil. The concentration of As in leachate of TCLP, KSLT, and old KSLT for soil are below the standard. However that in leachate of revised KSLT is above the standard. Because of extraction fluid used in revised KSLT is very strong acid. It is arsenic in s/s with binder should be exhaustingly leached. Therefore S/S process would not be available for As treatment in soil in Korea.