• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권1호
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• pp.49-62
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• 2023

Phthalates are animal carcinogens. Potassium hydrogen phthalate (KHP), which has the least complicated structure among phthalates, is used for the analysis of total organic carbon and formaldehyde. However, its toxicity has not been confirmed. A 24-hour acute toxicity test was performed using Daphnia magna, a water flea used to evaluate aquatic toxicity owing to its high sensitivity. The lowest observed effect concentration of KHP was found to be 240 mg/L. The effects of phosphorus, nitrogen, and Cr(6+), which are able to be discharged along with KHP, were also confirmed using tests. At 240 mg/L KHP, toxicity increased as phosphorus, nitrogen, and Cr(6+) increased. In addition, tests were performed to confirm the half maximal effective concentration of KHP. Through 10 test repetitions, the average ecotoxicity value was found to be 0.3, the average half maximal effective concentration was 327.75 mg/L, and the coefficient of variation (%) was 3.16%; because the latter value is lower than 25%, which is what is generally suggested for the water pollution standard method, the reproducibility of the tests is sufficient to replace the existing standard reference toxicity test that uses potassium dichromate. In addition, the half maximum effective concentration of potassium hydrogen phthalate is approximately 218 times more than that of potassium dichromate; therefore, toxicity is relatively low. In conclusion, KHP is a feasible alternative to the highly toxic potassium dichromate for performing the standard reference toxicity test.

• Ocean Science Journal
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• 제40권1호
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• pp.17-24
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• 2005

A series of experiments were conducted to develop standard test organisms and test protocols for measuring sediment toxicity using candidate amphipods such as Mandibulophoxus mai, Monocorophium acherusicum, Haustorioides indivisus, and Haustorioides koreanus, which are indigenous to Korea. The relevant association of test species with sediment substrates was one of the important factors in sediment bioassay. The indigenous amphipods M mai and M. acherusicum were well associated with test sediments when they were exposed to various sediment substrates from sand to mud. The tolerant limits to various physico-chemical factors affecting bioassay results such as temperature, salinity and ammonia, as well as sensitivities to reference toxicant and contaminated sediments, were investigated using M. mai and M. acherusicum in the present study. These amphipods were tolerant to relatively wide ranges of salinity $(10{\sim}30\;psu)$ and ammonia (<50 ppm), and displayed relevant sensitivity to temperature as well. They are more sensitive to Cd, the reference toxicant, when compared to the standard test species used in other countries. Field-sediment toxicity tests revealed that M. mai would be more sensitive to sediment-associated pollutants than M. acherusicum, while the sensitivity of M. acherusicum was comparable to that of Leptocheirus plumulosus, which has been used as a standard test species in the United States of America. Overall results of this first attempt to develop an amphipod sediment toxicity test protocol in Korea indicated that M. mai and M. acherusicum would be applicable in the toxicity assessment of contaminated sediments, following the further evaluation encompassing various ecological and toxicological studies in addition to test method standardization.

• 농약과학회지
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• 제12권3호
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• pp.222-228
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• 2008

벼재배용 농약의 담수 조류에 대한 독성과 감수성을 조사하고자 4종의 담수 조류를 이용하여 생장저해시험을 실시하였다. 시험농약은 벼재배용으로 사용되는 butachlor 5% GR, butachlor 33% EW, isoprothiolane 12% GR, isoprothiolane 40% EC, diazinon 3% GR, diazinon 34% EC이며, 시험농약을 담수 녹조류 3종(Selenastrum capricornutum, Scenedesmus subspicatus, Chlorella vulgris)과 규조류 1종(Nitzschia palea)에 72시간 노출시켜 그 생장률을 조사하였고 생장저해율을 이용하여 50% 영향농도($EC_{50}$)를 산출하였다. 시험 결과, 모든 시험농약에서 유제와 유탁제가 입제보다 담수 조류에 대한 독성이 높았으며, 특히 유제의 경우 농약 원제보다도 높은 독성이 나타났다. 이러한 결과로 농약 제제의 특성에 따라 수조류에 대한 독성이 높아지거나 낮아지고 있음을 알 수 있었다. 그리고 시험종간 감수성에 있어서는 대부분의 시험농약에서 녹조류인 S. capricornutum와 S. subspicatus는 비슷한 수준의 감수성을 나타내고 있는 반면 C. vulgris의 감수성은 확연히 낮았다. 규조류인 N. palea의 농약에 대한 감수성은 S. capricornutum와 S. subspicatus에 비해 다소 낮거나 비슷하였으나, C. vulgris에 비해 감수성이 높게 나타났다.

• 환경위생공학
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• 제9권2호
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• pp.101-109
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• 1994

Acute toxicity of heavy metals( mercury, cadmium lead, chromium and copper ), organophosphours insecticides( EPN, 2, 4- D, and parathion ), and other chemicals( arsenic, cyanide, and phenol ) to Daphnia magna was analyzed. Acute toxicity of heavy metals, organophosphours insecticides and other chemicals on Daphnia magna, LC$_{50}$ was showed 0.07-0.73mg/1, 0.22-1.94mg/t and 1.73-39.92mg/1 respectively. Daphnia magna to most of toxic chemicals was sensitive and then it seems to be useful except phenol in toxic test of water. Toxic chemicals were classified as Group 1 which had a high LC$_{50}$ value and the low increase rate of toxicity according to the increase of concentration, Group 2 which had a high LC$_{50}$ value and the high increase rate of toxicity, Group 3 which had a low LC$_{50}$ value and the low increase rate of toxicity. To Daphnia magna, lead, chromium, EPN, and parathion were included in Group 1; Mercury and copper in Group 2; Arsenic and cadmium in Group 3; Cyanide 2,4- D, and phenol in Group 4.

• 환경생물
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• 제30권4호
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• pp.314-318
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• 2012

The aims of the present study were to estimate the possibility for toxicity test and compare acute toxicity of potassium dichromate in the larva stage of Neocaridina denticulata, Daphnia magna and the juvenile stage of Oryzias latipes. N. denticulate, a freshwater shrimp lives in Korea, is an indigenous species and considered to be useful for toxicity test. D. magna and O. latipes were recommended as a test species for the OECD test guideline. The 96 h-$LC_{50}$ potassium dichromate value was 0.62 mg $L^{-1}$ for the larva stage of N. denticulata and 168.44 mg $L^{-1}$ for the juvenile stage of O. latipes. The 48 h-$EC_{50}$ value was 1.27 mg $L^{-1}$ for the D. magna. The study was confirmed higher sensitivity of the larva stage of N. denticulata to potassium dichromate compared to the D. magna and the juvenile stage of O. latipes.

• Environmental Analysis Health and Toxicology
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• 제14권4호
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• pp.135-144
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• 1999

Environmental toxicities of priority water pollutants were evaluated by two selected bioassays, Lettuce seed germination/elongation test and Microtox acute toxicity test. Toxic chemicals (heavy metals, polycyclic aromatic hydrocarbons, and phenolic compounds) inhibited the germination rate and root elongation of Lettuce seed, as well as the bioluminescence of Microtox bacteria. When test biota were exposed to target chemicals, the sensitivity of Lettuce bioassay was relatively lower than that of Microtox bioassay. However, Lettuce bioassay may be a good candidate for prescreening the environmental toxicities of priority water pollutants, since the testing method with Lettuce seed was relatively easier and more economic than with Microtox bacteria. Toxicity tests were conducted to compare the validity and sensitivity of both bioassays for sediment from a small stream passed through urban area as well as leachate from a municipal solid waste landfill. From experimental results, we found that Lettuce test and Microtox test are compensated each other as a battery of bioassay for evaluating the environmental toxicities of field samples obtained from a small stream contaminated by pollutants.

• 한국습지학회지
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• 제2권1호
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• pp.69-85
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• 2000

Wetlands are generally thought to be among the most fertile and productive ecosystems of the world. They provide a variety of ecological functions to the landscape. In recent years there has been considerable research activity to generate more scientific documentation on the ecological functions of wetlands. Many pollutants released to the environment settle and accumulate in the silt and mud called sediment on the bottoms of wetlands. Contaminated sediment can cause adverse effects to aquatic organism and eventually to ecological system. Sediment toxicity test with water fleas has been done by standardized preparation method of pore and elutriate water methods described in the literature for the need to protect Woopo wetlands. The results of Daphnia magna, Ceridaphnia dubla and Simocephalus sp. toxicity test were compared and discussed in terms of the relative sensitivity and discrimination abilities with both pore and elutriate water obtained from the sediments of Woopo wetlands.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.187-198
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• 2006

The aromatic hydrocarbons (AHs), which include benzene, polycyclic aromatic hydrocarbons, and dioxin, are important chemical and environmental contaminants in industry that usually cause various diseases. Over the years, numerous studies have described and evaluated the adverse health effects induced by AHs. Currently, "Omics" technologies, transcriptomics and proteomics, have been applied in AH toxicity studies. Proteomics has been used to identify molecular mechanisms and biomarkers associated with global chemical toxicity. It could enhance our ability to characterize chemical-induced toxicities and to identify noninvasive biomarkers. The proteomic approach (e.g. 2-dimensional electrophoresis [2-DE]), can be used to observe changes in protein expression during chemical exposure with high sensitivity and specificity. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and electrospray ionization-quadrupole (ESI-Q)-TOF MS/MS are recognized as the most important protein identification tools. This review describes proteomic technologies and their application in the proteomic analysis of AH toxicity.

• 한국환경보건학회지
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• 제45권3호
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• pp.285-294
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• 2019

Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
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• pp.94-99
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• 2000

Bioluminescence is being used as a prevailing reporter of gene expression in microorganisms and mammalian cells. Bacterial bioluminescence draws special attention from environmental biotechnologists since it has many advantageous characteristics, such as no requirement of extra substractes, highly sensitive, and on-line measurability. Using bacterial bioluminescence as a reporter of toxicity has replaced the classical toxicity monitoring technology of using fish or daphnia with a cutting-edge technology. Fusion of bacterial stress promoters, which control the transcription of stress genes corresponding to heat-shock, DNA-, or oxidative-damaging stress, to the bacterial lux operon has resulted in the development of novel toxicity biosensors with a short measurement time, enhanced sensitivity, and ease and convenient usage. Therefore, these recombinant bioluminescent bacteria are expected to induce bacterial bioluminescence when the cells are exposed to stressful conditions, including toxic chemicals. We have used these recombinant bioluminescent bacteria in order to develop toxicity biosensors in a continuous, portable, or in-situ measurement from for air, water, and soil environments. All the data obtained from these toxicity biosensors for these environments were found to be repeatable and reproducible, and the minimum detection level of toxicity was found to be ppb (part per billion) levels for specific chemicals.