• 한국환경보건학회지
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• 제38권4호
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• pp.300-310
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• 2012

Objectives: Indoor air quality problems in public spaces have recently become a very important issue in South Korea, because many spend a long time every day indoors. In this study, an extensive literature review was performed on the subject of suspended bacteria and fungi in public facilities. The objective of this study was to evaluate the extent and types of suspended bacteria and fungi in indoor air in South Korea. This study will provide direction to the research in the field of indoor air. Methods: Article reviews was conducted using a search engine and the following keywords; 'bacteria', 'total suspended bacteria', 'fungi' and 'indoor air' from published articles since 2000. Results: Most of the places of measurement were schools, kindergartens, childcare centers and general hospitals. The highest concentrations of total suspended bacteria and fungi were found in schools and kindergartens. Some public facilities exceeded the standard of 800 CFU/$m^3$. There were few studies on other suspended bacteria: Gram Negative Bacteria, Staphylococcus and Klebsiella pneumoniae. Conclusions: It appears that more studies with intensive controls are needed. It was found that schools and kindergartens were most susceptible to microbial indoor air pollution. These results may be able to suggest an indoor standard for suspended bacteria and fungi in South Korea.

• 한국산업보건학회지
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• 제18권1호
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• pp.11-19
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• 2008

Mean levels of airborne bacteria, airborne fungi, temperature, relative humidity and carbon dioxide in total 69 general offices were $426({\pm}83)\;cfu/m^3$, $234({\pm}125)\;cfu/m^3$, $25.9({\pm}1.3)\;^{\circ}C$, $57.7({\pm}8.6)\;%$, $422({\pm}38)\;ppm$, respectively. The I/O ratio of airborne bacteria and fungi was over 1 and there was no significant difference among temperature, relative humidity and carbon dioxide in total 69 general offices. In construction period, a concentration of airborne bacteria and fungi was significantly highest in general offices constructed under one year and over three years since construction, respectively (p<0.05). The concentration of airborne fungi in general offices located at basement was significantly higher than those located at ground (p<0.05). No significant difference of airborne bacteria and fungi in general offices was found regardless of installation of HVAC system (p>0.05). The dominant bacterial genera identified in general offices was Staphylococcus, followed by Micrococcus, Bacillus, and Corynebacterium while usarium, Penicillium, Aspergillus, Alternaria, Rhizopus and Mucor were identified as dominant fungal genera in general offices.

• 한국환경과학회지
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• 제29권11호
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• pp.1109-1123
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• 2020

In this study, the amount of harmful fungi and bacteria contained in the drainage and culture medium from the paprika hydroponic facility is identified. In addition, by proposing the necessity of effective purification of discharged drainage, this study attempted to confirm the possibility of drainage reuse. Finally, this study provides basic data on the basis for calculating the need for purification facilities in the future, as well as improvements in horticulture facility for sustainable agriculture. As a result of the analysis, a total of 12 types of fungi were detected in paprika medium and 10 types of fungi were detected in the drainage, and their densities were 130 and 68, respectively. Among the fungi detected in the media and drainage of the paprika hydroponic facility, the fungi with the highest detection frequency are Fusarium, Phytophthora, and Pythium. In the case of bacteria, a total of 2 types of bacteria were detected in the paprika facilities, and the density was 28 and 23, respectively. Therefore, in order to reuse the drainage and settle the circulating hydroponic cultivation system, a water treatment process capable of appropriate treatment is required.

• Mycobiology
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• 제40권1호
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• pp.8-13
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• 2012

In the present study, an attempt to evaluate the antimicrobial and antioxidant activity of fungal endophytes inhabiting $Emblica$ $officinalis$ has been made keeping in view the medicinal importance of the selected host plant in Indian traditional practices. A total of four endophytic fungi belonging to Phylum Ascomycetes were isolated from different parts of the plant which were characterized morphologically and by using rDNA-internal transcribed spacer. The most frequently isolated endophyte was $Phomopsis$ sp. The antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay, and total phenol were evaluated using ethanolic extract of endophytic fungi. DPPH activities in all the ethanolic extract increased with the increase in concentrations. Endophytes, $Phomopsis$ sp. and $Xylaria$ sp. showed highest antioxidant activity and also had the higher levels of phenolics. Antimicrobial activity of fungal extract were tested against four bacteria namely, $Escherichia$ $coli$ MTCC730, $Enteroccocus$ $faecalis$ MTCC2729, $Salmonella$ $enterica$ ser. $paratyphi$ MTCC735 and $Streptococcus$ $pyogenes$ MTCC1925, and the fungus $Candida$ $albicans$ MTCC183. In general, the fungal extracts inhibited the growth of test organisms except $E.$ $coli$.

• 한국균학회지
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• 제21권1호
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• pp.77-84
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• 1993

Out of 210 tinea capitis cases studies, 16 were negative when examined with KOH, among the remaining 194 cases, 123 were males (63.4%) and 71 were females (36.6%) and the age of incidence ranged between 7 and 15 years. Microsporum was the main causal agent being identified in 82 cases (42.3%) and was represented by 5 species among which M. canis was common in Egypt (55 cases, 28.4%). Trichophyton constituted 32% and was represented by 8 species among which T. violaceum was the most common (24.2% of total cases). Candida were isolated from 3.6% of total cases. The 47 species and twenty-five genera from nondermatophyte-cyclohexamide resistant fungi were recovered from the diseased skin tissue. Penicillium and Aspergillus were the most abundant followed by Scopulariopsis, Alternaria, Thermoascus, Chrysosporium and Cladosporium. Studies of the air-borne fungi in-door the hospital wards revealed the occurrence of 57 species belonging to 28 genera, among which Aspergillus flavus, Penicillium chrysogenum, p. corylophilum, A. niger, Tritirachium rosum and Alternaria alternata were the most common. Results of the out-door experiments were basically similar to those of the in-door experiments.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.30-30
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• 2015

This study was conducted aiming with the assessment of fungal diversity in soil samples collected from different locations of Jeollabuk-do and Chungcheongbuk-do, Korea. Forty soil samples were collected in 2015 and fungi were isolated through serial dilution technique. Isolated fungi were purified and differentiated according to their morphological and microscopic characteristics. In total, 150 different representative isolates were recovered and the genomic DNA of each isolate was extracted by using QIAGEN$^{(R)}$ Plasmid Mini Kit (QIAGEN Sciences, USA) and the identification of fungi was carried out by sequence analysis of internal transcribed spacer (ITS) region of the 18S ribosomal DNA (18S rDNA). Recovered isolates belonged to 37 family, 67 genera and 108 species. Aspergillus spp., Penicillium spp., Trichoderma spp., Chaetomium spp. And Fusarium spp. were the most dominant taxa in this study. Out of total species, 20 species were identified as new records for Korea.

• Mycobiology
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• 제39권4호
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• pp.278-282
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• 2011

The objective of this study was to find useful fungi with ${\alpha}$-amylase activity from the Korean traditional nuruk for the quality of traditional Korean alcoholic beverage. In this study, 165 samples of traditional nuruk were collected from 170 regions throughout Korea and the fungi were isolated to a total of 384 strains. In order to investigate the effect of microflora on nuruk, ${\alpha}$-amylase activity, saccharogenic power (SP), starch hydrolysis activity and acid producing activity were evaluated. Ten strains were selected by ${\alpha}$-amylase activity, which ranged from 458.47 to 1,202.75 U/g. The size of the discolored zone for the starch hydrolysis activity of each fungus ranged from 0.3 to 2 cm. The SP of the 10 strains ranged from 228.8 to 433.4 SP. Of the 10 stains, three were identified as Aspergillus oryzae, two as Aspergillus flavus, two as Lichtheimia sp., one as Rhizopus oryzae and two as other strains. The total aflatoxins present in the nuruks were examined using enzyme-linked immunosorbent assay. The 10 nuruks had less than 1.11 ppb of aflatoxins.

• Journal of Korean Society for Atmospheric Environment
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• 제23권E1호
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• pp.1-9
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• 2007

In Korea, there is only a limited amount of information currently available on the levels of airborne bacteria and fungi of cattle sheds, although certain portions of people are potentially exposed to these bioaerosols in cattle sheds. Accordingly, the current study measured them inside cattle sheds, inside and outside farmers' houses near the sheds, and/or inside residential houses far away from the sheds during winter, 2004 and summer, 2005. The airborne bacteria and fungi were detected in most samples in the cattle farmers' houses as well as in the cattle sheds. Aspergillus, Cladosporium, and Penicillium, which have been associated with adverse health effects, were three most prevalent fungal genera, and they took most of the total fungi (more than 69%). The microbial concentrations measured inside the cattle sheds were comparable to those in other reports. Nevertheless, the present arithmetic and geometric mean (GM) microbial concentrations exceeded the Korean guideline for total airborne bacteria at medical facilities ($800\;CFU\;m^{-3}$), the current GM residential indoor concentrations at houses, and the residential indoor levels reported in other countries. The present findings suggest the need for a strategy to reduce Korean cattle farmers' exposure to these microorganisms. In contrast to the microbes, it is suggested that the cattle shed is not an important microenvironment for $PM_{10}$ exposure. Two characteristics examined in this study (seasonal variation and summer survey period, i.e., temperature and humidity) were all important for the cattle farmers' occupational exposure to airborne microbes. The lack of constancy between highest and lowest concentrations of bioaerosols over the survey period further suggests the necessity of performing a long-term survey to better examine farmer exposure levels and their variability.

• 한국균학회지
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• 제49권4호
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• pp.497-505
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• 2021

다양한 지역에서 채취한 참당귀(Angelica gigas Nakai)의 잎, 줄기, 뿌리에서 내생균을 분리하였다. 내생균 균주는 형태적특징과 ITS (internal transcribed spacer) 지역의 염기서열을 이용하여 동정하였다. 총 35종의 내생균이 동정되었다. 내생균의 다양성은 참당귀의 조직에 따라 다르게 나타났다. 분리된 내생균은 참당귀 점무늬병균 Phoma sp. Y11 균주와 대치배양하여 항균활성을 측정하였다. 총 14종의내생균이균주 Y11에대해억제효과를나타내었다. 본 연구는 참당귀에서 분리한 내생균이 참당귀의 점무늬병에 대한 생물학적 방제제로 사용될 수 있다는 것을 제시하고 있다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.41-41
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• 2014

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is a serious pathogenic worm that quickly dry pine trees to death. Recently, PWN has been devastating huge amounts of conifer trees in Korea. As a first step to explore the association and ecological roles of fungi in PWN life cycle in Korea, in this study we first isolated and indentified fungi from PWN-infested Korean pine and Japanese black pine wood sampled in Jinju, Sacheon, Pocheon, Chuncheon, Gwangju, and Hoengseong in Korea. A total of 144 fungal isolates were obtained from Japanese black pine wood and 264 fungal isolates from Korean pine wood. Their morphology and nucleotide sequences of the ITS rDNA and ♌-tubulin gene were examined for species identification. Ophiostoma ips, Botrytis anthophila, Penicillium sp., Hypocrea lixii, Trichoderma atroviride, O. galeiforme, Fusarium proliferatum were identified from Japanese black pine wood. Leptographium koreanum, L. pini-densiflorae, Ophiostoma ips, Penicillium raistrick, Trichoderma sp. were isolated from Korean pine wood. O. ips and L. koreanum were the major species on the two different PWN-infected pine tree. The cultivation of PWN on fungal mat of the identified species did some enhance PWN reproduction. The ambrosia beetle, Platypus koryoensis, is a serious pest of oak trees in Korea. In this study we investigated filamentous fungi present in the body of the beetle. Fourteen genera of filamentous fungi belonging to Ascomycota and Basidiomycota were isolated. All the obtained genera were isolated in the mitosporic state. The identified fungi were classified in 11 distinct orders including the Ascomycota (Eurotiales, Hypocreales, Microascales, Ophiostomatales, Pleosporales, and Sordiales) and Basidiomycota (Agaricales, Corticiales, Polyporales, and Russulales Xylariales). Within Ascomycota, 13 species were found. Meanwhile five species were found within Basidiomycota. The results showed the presence of diverse fungi in P. koryoensis. Among the isolated fungi, some were able to produce wood degrading enzymes. Further fungal isolation was performed with P. koryoensis infested Quercus mongolica trees sampled at Kumdan mountain in Hanam-Si, Gyeonggi province from June of 2009 to June of 2010. Penicillin spp. and Trichoderma spp. were the major species of mold fungi group. Pichia guilliermondii was the major species of mold yeast group. Raffaelea quercus-mongolicae was also isolated, but its isolation frequency was not high. Other species identified were Ambrosiella xylebori, Fusarium solani, Cryphonectria nitschke, Chaetomium globosum, and Gliocladium viride, Candida kashinagacola, C. maritima, C. vanderkliftii, Saccharomycopsis crataegensis.